LOCUS       V008292                 5833 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008292
VERSION     V008292
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Raja Abd Rahman RNZ., Wong CF, Salleh AB, Basri M.
  TITLE     Expression vector of pCon4
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Raja Abd Rahman RNZ., Wong CF, Salleh AB, Basri M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2011) Department of Microbiology, Universiti Putra
            Malaysia, Faculty of Biotechnology and Biomolecular Sciences, UPM
            Serdang, Selangor 43400, Malaysia
REFERENCE   3  (bases 1 to 5833)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5833)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JAN-2011) Department of Microbiology, Universiti Putra Malaysia,
            Faculty of Biotechnology and Biomolecular Sciences, UPM Serdang,
            Selangor 43400, Malaysia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(62..650)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(824..1681)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1682..1786)
                     /label="AmpR promoter"
     CDS             complement(2081..3574)
                     /gene="lasB"
                     /label="Elastase"
                     /note="Elastase from Pseudomonas aeruginosa (strain ATCC
                     15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C
                     / PRS 101 / PAO1). Accession#: P14756"
     misc_feature    complement(3619..3648)
                     /label="operator O3 of PT7(A1/O4/O3)"
                     /note="operator O3 of PT7(A1/O4/O3)"
     protein_bind    3630..3646
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    complement(3656..3661)
                     /label="promoter -10 of PT7(A1/O4/O3)"
                     /note="promoter -10 of PT7(A1/O4/O3)"
     protein_bind    complement(3663..3679)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    complement(3679..3684)
                     /label="promoter -33 of PT7(A1/O4/O3)"
                     /note="promoter -33 of PT7(A1/O4/O3)"
     rep_origin      complement(4413..4764)
                     /direction=LEFT
                     /label="pRO1600 oriV"
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             4778..5608
                     /label="pRO1600 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     primer_bind     5772..5788
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 ctgcaggagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
       61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      481 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      961 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1801 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1861 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     1921 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     1981 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     2041 agcagattgt actgagagtg caccatatga agctttttta caacgcgctc gggcaggtca
     2101 cgccgacggt gctgaacgcc cgggtgacgt cagccgccga gtagttgcgg ttctgcgccg
     2161 agcgaatcac cccgcaggcg ccgctgttgt agttgctggt ggcggtccag tagtagcggt
     2221 tggcgtcgac gaacacctcg aaggccttgc gggtatccca gcccggcgaa ttggccaaca
     2281 ggtagaacgc acggttgtac acgccgctgg agtggtgcac gtcgatgccg ttgtagtact
     2341 gcgacgcgtt gtcgatggat cgcccgtcgc ggctgggctg gtccatgtag cgcagcgcac
     2401 cgctgccctt cttgatgtcg tagccgatca ggaagtcgtt cttgccgcgc atgtagaatt
     2461 cggctgcctc gccggccatg tcggagaacg cttcgttcat tccgcctgat tgcccgcggt
     2521 agatcagccc ggagttctgc tcggtaaagc cgtggctgac ctcgtgggcc gccacgtcca
     2581 gcgacaccag cggatagaac atggtggcgc cgtcgccgaa gagcatcgcc gtgccgcccc
     2641 agtaggcgtt ctccacgctg cgcccgtagt gcaccttcat gtacagcttg tgggtcagcg
     2701 ggctggtgcc gaaccagtcc cggtacagtt tgaacaccac gccgccgaag aaatgcgcgt
     2761 cgttcagcgg cgaataggcg ccgttgacct gcttgtaggt gttggtcggg caggcgaagc
     2821 ggaacggcgt ggtcttgctg tcgtcggtgc tgccgttcat gtcgacggtg atgacgttgc
     2881 cgtcgtccat ctcgcagcgg tcgttgacga tcagcggacc gtagtcgcta ccgtaggtgt
     2941 acttgccgat cttctggttg ccgccggggc cgcccgcctc ggcgtgggcc aggccttccc
     3001 actgatcgag cacttcgccg gtcttggcgt cgatgacgaa atgcggccgc gacagtccct
     3061 cgccgggaat caggtaggag acgttgtaga ccagttgggc gatgttgctc tcgcccaggc
     3121 ggatcaccag ttccactttg tcattctcgg tcttgcggcc ctgggccttc aggctcttgg
     3181 cctgggccag cacctgctcg gcggataccg ccgcggtggt gctgcccggc aggtcggcag
     3241 cgatgttggc gacgaaatgg ccgctgcgct gcgccgccac gctcttgccg ggacccttga
     3301 cttcggtgat ggcttcgccg accacccgta cgccgttgtg gaattgctcg tagcgggtga
     3361 cctgcttgcc gttgggcagg gtcgtgctgc ggatcgcttt cagttcatcg gcaccgccag
     3421 cgccgaccgc ggcttgcaag gtgaccgggc cgggcgcgcc ctgggcagcc ttgctgggga
     3481 gtttggacac gtcgatcagg tcggcggcaa gagcggccgg cgaaacaccc atgatcgcaa
     3541 cgaacaacag gtcaagcgta gaaaccttct tcattctgca ggtcgactct agaggatccc
     3601 cgggtaccga gctcgaattc tgtgtgaaat tgttatccgc tcacaattga atctaagtat
     3661 cattgttatc cgctcacaag tcaacactct ttttgataaa ttttcatatg cggtgtgaaa
     3721 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gcaatggcaa caacgttgcg
     3781 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     3841 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     3901 tgctgataaa tctggagccg gtgagcgtgg atctcgcggt atcattgcag cactggggcc
     3961 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4021 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4081 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4141 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4201 gttccactga gcgtcagacc ccaattacac gccactggct gtgcttgctg gggtgacggt
     4261 ggcaacggtg gcggccttgc tgggctatcg cgttggaaag aaacgaggga aaggggactg
     4321 ataaaccggt cttagcccct ccccttggtg tccaaccgct ctgtaggcct ctcaggcgcc
     4381 gctggtgccg ctggttggac gccaagggtg aatccgcctc gataccctga ttactcgctt
     4441 cctgcgccct ctcaggcggc gataggggac tggtaaaacg gggattgccc agacgcctcc
     4501 cccgcccctt caggggcaca aatgcggccc caacggggcc acgtagtggt gcgttttttg
     4561 cgtttccacc cttttcttcc ttttcccttt taaacctttt aggacgtcta caggccacgt
     4621 aatccgtggc ctgtagagtt taaaaaggga cggatttgtt gccattaagg gacggatttg
     4681 ttgttaagaa gggacggatt tgttgttgta aagggacgga tttgttgtat tgtgggacgc
     4741 agatacagtg tccccttata cacaaggaat gtcgaacgtg gcctcacccc caatggttta
     4801 caaaagcaat gccctggtcg aggccgcgta tcgcctcagt gttcaggaac agcggatcgt
     4861 tctggcctgt attagccagg tgaagaggag cgagcctgtc accgatgaag tgatgtattc
     4921 agtgacggcg gaggacatag cgacgatggc gggtgtccct atcgaatctt cctacaacca
     4981 gctcaaagaa gcggccctgc gcctgaaacg gcgggaagtc cggttaaccc aagagcccaa
     5041 tggcaagggg aaaagaccga gtgtgatgat taccggctgg gtgcaaacaa tcatctaccg
     5101 ggagggtgag ggccgtgtag aactcaggtt caccaaagac atgctgccgt acctgacgga
     5161 actcaccaaa cagttcacca aatacgcctt ggctgacgtg gccaagatgg acagcaccca
     5221 cgcgatcagg ctttacgagc tgctcatgca atgggacagc atcggccagc gcgaaataga
     5281 aattgaccag ctgcgaaagt ggtttcaact ggaaggccgg tatccctcga tcaaggactt
     5341 caagttgcga gtgcttgatc cagccgtgac gcagatcaac gagcacagcc cgctacaggt
     5401 ggagtgggcg cagcgaaaga ccgggcgcaa ggtcacacat ctgttgttca gttttggacc
     5461 gaagaagccc gccaaggcgg tgggtaaggc cccagcgaag cgcaaggccg ggaagatttc
     5521 agatgctgag atcgcgaaac aggctcgccc tggtgagaca tgggaagcgg cccgcgctcg
     5581 actaacccag atgccgctgg atctggccta gaggccgtgg ccaccacggc ccggcctgcc
     5641 tttcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     5701 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     5761 gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtacccggg gatcctctag
     5821 agtcgacaca tgt
//
