LOCUS       40924_12725        4067 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Phagemid vector pComb3X-KD247scFv-VI, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ong YT, Kirby KA, Sarafianos SG.
  TITLE     Genetic modification of KD-247 scFv to improve affinity for the V3 
            loop of HIV-1 from multiple clades
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Ong YT, Kirby KA, Sarafianos SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2014) Molecular Microbiology and Immunology, 
            University of Missouri, 401 Bond LSC, 1201 E. Rollins St., Columbia,
            MO 65211, USA
REFERENCE   3  (bases 1 to 4067)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4067)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-NOV-2014) Molecular Microbiology and Immunology, University of 
            Missouri, 401 Bond LSC, 1201 E. Rollins St., Columbia, MO 65211, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    109..130
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        145..175
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    183..199
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     sig_peptide     222..284
                     /label=OmpA signal peptide
                     /note="signal peptide from the E. coli outer membrane
                     protein OmpA"
     CDS             1056..1073
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             1080..1106
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     rep_origin      complement(1682..2137)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2164..2268
                     /label=AmpR promoter
     CDS             2269..3126
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3300..3888
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
       61 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      121 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      181 aattgtgagc ggataacaat tgaattcagg aggaatttaa aatgaaaaag acagctatcg
      241 cgattgcagt ggcactggct ggtttcgcta ccgtggccca ggcggcccag gttcagctgg
      301 tacaaagcgg tgcagaagtc aagaaaccgg gcgcaagcgt aaaagtctct tgcaaggcaa
      361 gtgggtatac gttcacaaat tcttggatcg gttggttccg tcaggcgccg gggcaaggcc
      421 tggaatggat tggcgatatt tatccgggtg gtgggtacac aaactacaat gagatcttca
      481 aaggtaaagc cacgatgacc gcggatacct ctaccaatac cgcgtacatg gaactgtctt
      541 ctctgcgcag cgaagacact gcagtgtact actgctctcg cggcatccca ggctatgcta
      601 tggattattg gggccaaggt accctggtga cggtttctag tggtggcggt gggtctggcg
      661 gtggtggcag cggtggcggt ggcagcggcg gtggtggttc tgatattcag atgacgcagc
      721 gtccggacag cctgagcgcc tctgtgggtg atcgcgtgac gatgagttgt aagagctccc
      781 agagtctgct gaacagcggt gatcagaaga actatctgac ctggtatcag cagaaaccgg
      841 gccaaccgcc aaagcttctg atttattggg cgagtaccgg cgaaagcggg gtcccagatc
      901 gttttagcgg gagcgggagt gggaccgatt ttacatttac aattagtagt ctgcaacctg
      961 aggacatcgc tacttattac tgtcagaacg actatagtta tccttggact tttggccagg
     1021 gtactaaagt tgagatcaag ggccaggccg gccagcacca tcaccatcac catggcgcat
     1081 acccgtacga cgttccggac tacgcttctt aggagggtgg tggctctgag ggtggcggtt
     1141 ctgagggtgg cggctctgag ggaggcggtt ccggtggtgg ctctggttcc ggtgattttg
     1201 attatgaaaa gatggcaaac gctaataagg gggctatgac cgaaaatgcc gatgaaaacg
     1261 tgctacagtc tgacgctaaa ggcaaacttg attctgtcgc tactgattac ggtgctgcta
     1321 tcgatggttt cattggtgac gtttccggcc ttgctaatgg taatggtgct actggtgatt
     1381 ttgctggctc taattcccaa atggctcaag tcggtgacgg tgataattca cctttaatga
     1441 ataatttccg tcaatattta ccttccctcc ctcaatcggt tgaatgtcgc ccttttgtct
     1501 ttggcgctgg taaaccatat gaattttcta ttgattgtga caaaataaac ttattccgtg
     1561 gtgtctttgc gtttctttta tatgttgcca cctttatgta tgtattttct acgtttgcta
     1621 acatactgcg taataaggag tcttaagcta gctaattaat ttaagcggcc gcagatctgg
     1681 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     1741 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     1801 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     1861 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     1921 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     1981 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     2041 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     2101 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     2161 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2221 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2281 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2341 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2401 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2461 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2521 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2581 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2641 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2701 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2761 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2821 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2881 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2941 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     3001 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     3061 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3121 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3181 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3241 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3301 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3361 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3421 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3481 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3541 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3601 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3661 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3721 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3781 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3841 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3901 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3961 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4021 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcgga
//
