LOCUS       40924_12575        6007 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCntrlPIGver2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Gurskaia NG, Staroverov DB, Fradkov AF, Luk'ianov KA.
  TITLE     [Coding region of far-red fluorescent protein katushka contains a 
            strong donor splice site]
  JOURNAL   Bioorg. Khim. 37 (3), 425-428 (2011)
  PUBMED    21899059
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Gurskaya NG, Staroverov DB, Zhang L, Fradkov AF, Markina NM, 
            Pereverzev AP, Lukyanov KA.
  TITLE     Analysis of alternative splicing of cassette exons at single-cell 
            level using two fluorescent proteins
  JOURNAL   Nucleic Acids Res. 40 (8), E57 (2012)
  PUBMED    22259036
REFERENCE   3  (bases 1 to 6007)
  AUTHORS   Gurskaya NG, Staroverov DB, Zhang L, Fradkov AF, Markina NM, 
            Pereverzev AP.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2015) Department of Genetics and Postgenomic 
            Technologies Laboratory of Biophotonics, M.M. Shemyakin and Yu.a. 
            Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy
            of Sciences, Ulitsa Miklukho-Maklaya, 16/10, Moscow 117997, Russian 
            Federation
REFERENCE   4  (bases 1 to 6007)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6007)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioorg. 
            Khim."; date: "2011"; volume: "37"; issue: "3"; pages: "425-428"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012"; volume: "40"; issue: "8"; pages: "E57"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (22-SEP-2015) Department of Genetics and Postgenomic Technologies 
            Laboratory of Biophotonics, M.M. Shemyakin and Yu.a. Ovchinnikov 
            Institute of Bioorganic Chemistry of the Russian Academy of 
            Sciences, Ulitsa Miklukho-Maklaya, 16/10, Moscow 117997, Russian 
            Federation"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    590..612
                     /note="multiple cloning site; enhancer; TATA box"
     CDS             613..1317
                     /label=TurboFP635
                     /note="far-red-shifted derivative of red fluorescent
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
     CDS             1957..2670
                     /label=TagGFP2
                     /note="monomeric green fluorescent protein, also known as 
                     mTagGFP"
     polyA_signal    2795..2916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2923..3378)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3405..3509
                     /label=AmpR promoter
     promoter        3511..3868
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3903..4694
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4929..4976
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5305..5893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtggg tgaggatagc gtgctgatca ccgagaacat gcacatgaaa
      661 ctgtacatgg agggcaccgt gaacgaccac cacttcaagt gcacatccga gggcgaaggc
      721 aagccctacg agggcaccca gaccatgaag atcaaggtgg tcgagggcgg ccctctcccc
      781 ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt tatcaaccac
      841 acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac atgggagagg
      901 atcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccagaac
      961 ggctgcctca tctacaacgt caagatcaac ggggtgaact tcccatccaa cggccctgtg
     1021 atgcagaaga aaacactcgg ctgggaggcc agcaccgaga tgctgtaccc cgctgacagc
     1081 ggcctgagag gccatagcca gatggccctg aagctcgtgg gcgggggcta cctgcactgc
     1141 tccctcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcttc
     1201 tacttcgtgg acaggagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag
     1261 cagcacgaga tggctgtggc tcgctactgc gacctgccta gcaaactggg gcacagctcc
     1321 ggactcaaga tctccggaaa tgttcaggct ggagactatg tgctaatcca tgcaggactg
     1381 agtggtgtgg gcacagctgc tatccaactc acccggatgg ctggagctat tcctctggtc
     1441 acagctggct cccagaagaa gcttcaaatg gcagaaaagc ttggagcagc tgctggattc
     1501 aattacaaaa aagaggattt ctctgaagca acgctgaaat tcaccaaagg tgctggagtt
     1561 aatcttattc tagactgcat aggcggatcc tactgggaga agaacgtcaa ctgcctggct
     1621 cttgatggtc gatgggttct ctatggtctg atgggaggag gtgacatcaa tgggcccctg
     1681 ttttcaaagc tactttttaa gcgaggaagt ctgatcacca gtttgctgag gtctagggac
     1741 aataagtaca agcaaatgct ggtgaatgct ttcacggagc aaattctgcc tcacttctcc
     1801 acggagggcc cccaacgtct gctgccggtt ctggacagaa tctacccagt gaccgaaatc
     1861 caggaggccc ataagtacat ggaggccaac aagaacatag gcaagatcgt cctggaactg
     1921 ccccagacgg tcgaccggga tccaccggtc gccaccatga gcgggggcga ggagctgttc
     1981 gccggcatcg tgcccgtgct gatcgagctg gacggcgacg tgcacggcca caagttcagc
     2041 gtgcgcggcg agggcgaggg cgacgccgac tacggcaagc tggagatcaa gttcatctgc
     2101 accaccggca agctgcccgt gccctggccc accctggtga ccaccctctg ctacggcatc
     2161 cagtgcttcg cccgctaccc cgagcacatg aagatgaacg acttcttcaa gagcgccatg
     2221 cccgagggct acatccagga gcgcaccatc cagttccagg acgacggcaa gtacaagacc
     2281 cgcggcgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcaag
     2341 gacttcaagg aggacggcaa catcctgggc cacaagctgg agtacagctt caacagccac
     2401 aacgtgtaca tccgccccga caaggccaac aacggcctgg aggctaactt caagacccgc
     2461 cacaacatcg agggcggcgg cgtgcagctg gccgaccact accagaccaa cgtgcccctg
     2521 ggcgacggcc ccgtgctgat ccccatcaac cactacctga gcactcagac caagatcagc
     2581 aaggaccgca acgaggcccg cgaccacatg gtgctcctgg agtccttcag cgcctgctgc
     2641 cacacccacg gcatggacga gctgtacagg taagcggccg cgactctaga tcataatcag
     2701 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2761 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2821 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2881 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata
     2941 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3001 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3061 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3121 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3181 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3241 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3301 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3361 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3421 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3481 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     3541 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3601 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3661 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3721 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3781 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3841 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     3901 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3961 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4021 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4081 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4141 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4201 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4261 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4321 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4381 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4441 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4501 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4561 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4621 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4681 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     4741 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4801 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4861 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     4921 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     4981 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     5041 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     5101 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     5161 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5221 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5281 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5341 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5401 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5461 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5521 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5581 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5641 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5701 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5761 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5821 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5881 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5941 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6001 catgcat
//
