LOCUS       40924_11286        3503 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Yeast knockout vector pCloneBle1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Spirek M, Carnecka M, Gregan J.
  TITLE     New plasmids for high-throughput gene deletions in 
            Schizosaccharomyces pombe
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Spirek M, Carnecka M, Gregan J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2009) Chromosome Biology, University of Vienna, 
            Viehmarktgasse 2a, Wien 1030, Austria
REFERENCE   3  (bases 1 to 3503)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3503)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUL-2009) Chromosome Biology, University of Vienna, 
            Viehmarktgasse 2a, Wien 1030, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            115..1036
                     /label=bleMX6
                     /note="yeast selectable marker conferring phleomycin
                     resistance"
     promoter        complement(1141..1159)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1417..2005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2179..3036)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3037..3141)
                     /label=AmpR promoter
     promoter        3487..3503
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttctcgag tgtacaccat ggggatcccc gggttaatta
       61 aggcgcgcca gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
      121 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
      181 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
      241 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
      301 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
      361 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
      421 ggatacagtt ctcacatcac atccgaacat aaacaaccat ggccaagttg accagtgccg
      481 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
      541 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
      601 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
      661 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
      721 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
      781 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgatcagtac
      841 tgacaataaa aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt
      901 agttgttcta ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat
      961 ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat
     1021 gctggtcgct atactgctgt cgattcgata ctaacgccgc catccagttt aaacgagctc
     1081 gaattcatcg atgatatcag atccactagt ggcctatgcg gccgcggatc tgccggtctc
     1141 cctatagtga gtcgtattaa tttcgataag ccaggttaac ctgcattaat gaatcggcca
     1201 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1261 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1321 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1381 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1441 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1501 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1561 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     1621 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1681 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1741 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1801 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     1861 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1921 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1981 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2041 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2101 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2161 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2221 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2281 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2341 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2401 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2461 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2521 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2581 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2641 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2701 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2761 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2821 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2881 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2941 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3001 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3061 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3121 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     3181 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     3241 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     3301 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     3361 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     3421 tatggacata ttgtcgttag aacgcggcta caattaatac ataaccttat gtatcataca
     3481 catacgattt aggtgacact ata
//
