LOCUS       V016396                 9726 bp    DNA     circular SYN 16-MAY-2020
DEFINITION  Exported.
ACCESSION   V016396
VERSION     V016396
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Guo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2019) Jiangnan University, State Key Laboratory of
            Food Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,
            China
REFERENCE   2  (bases 1 to 9726)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (28-OCT-2019) Jiangnan University, State Key Laboratory of Food
            Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,
            China"
FEATURES             Location/Qualifiers
     source          1..9726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             119..907
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      1161..1899
                     /label="CloDF13 ori"
                     /note="Plasmids containing the CloDF13 (CDF) origin of
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        1953..1987
                     /label="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     CDS             2137..2856
                     /gene="ubiG"
                     /label="Ubiquinone biosynthesis O-methyltransferase"
                     /note="Ubiquinone biosynthesis O-methyltransferase from
                     Escherichia coli (strain ATCC 8739 / DSM 1576 / NBRC 3972 /
                     NCIMB 8545 / WDCM 00012 / Crooks). Accession#: B1IXV6"
     CDS             2883..3893
                     /gene="rssB"
                     /label="Regulator of RpoS"
                     /note="Regulator of RpoS from Escherichia coli (strain
                     K12). Accession#: P0AEV1"
     CDS             3897..3914
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      3981..4028
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     protein_bind    complement(4064..4097)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4124..5134
                     /gene="rssB"
                     /label="Regulator of RpoS"
                     /note="Regulator of RpoS from Escherichia coli (strain
                     K12). Accession#: P0AEV1"
     CDS             5138..5155
                     /label="6xHis"
                     /note="6xHis affinity tag"
     regulatory      5222..5269
                     /label="T7 terminator"
                     /note="T7 terminator"
                     /regulatory_class="terminator"
     CDS             5436..6425
                     /gene="rpoS"
                     /label="RNA polymerase sigma factor RpoS"
                     /note="RNA polymerase sigma factor RpoS from Escherichia
                     coli (strain K12). Accession#: P13445"
     CDS             6435..6452
                     /label="6xHis"
                     /note="6xHis affinity tag"
     regulatory      6519..6566
                     /label="T7 terminator"
                     /note="T7 terminator"
                     /regulatory_class="terminator"
     protein_bind    complement(6598..6631)
                     /label="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are
                     compatible with each other, but incompatible with loxP or
                     loxN sites (Lee and Saito, 1988)."
     CDS             6658..7851
                     /gene="fabV"
                     /label="Trans-2-enoyl-CoA reductase [NADH]"
                     /note="Trans-2-enoyl-CoA reductase [NADH] from Clostridium
                     acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG
                     5710 / VKM B-1787). Accession#: Q97LU2"
     gene            7879..8727
                     /gene="hbd"
                     /label="hbd"
     CDS             7879..8727
                     /codon_start=1
                     /transl_table=11
                     /gene="hbd"
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /label="hbd"
                     /protein_id="QJR97829.1"
                     /translation="MKKVCVIGAGTMGSGIAQAFAAKGFEVVLRDIKDEFVDRGLDFIN
                     KNLSKLVKKGKIEEATKVEILTRISGTVDLNMAADCDLVIEAAVERMDIKKQIFADLDN
                     ICKPETILASNTSSLSITEVASATKTNDKVIGMHFFNPAPVMKLVEVIRGIATSQETFD
                     AVKETSIAIGKDPVEVAEAPGFVVNRILIPMINEAVGILAEGIASVEDIDKAMKLGANH
                     PMGPLELGDFIGLDICLAIMDVLYSETGDSKYRPHTLLKKYVRAGWLGRKSGKGFYDYS
                     K"
     CDS             8755..9537
                     /gene="crt"
                     /label="Short-chain-enoyl-CoA hydratase"
                     /note="Short-chain-enoyl-CoA hydratase from Clostridium
                     acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG
                     5710 / VKM B-1787). Accession#: P52046"
     CDS             9547..9564
                     /label="6xHis"
                     /note="6xHis affinity tag"
     regulatory      9632..9678
                     /label="T7 terminator"
                     /note="T7 terminator"
                     /regulatory_class="terminator"
ORIGIN
        1 ttcatgtgca gctccataag caaaagggga tgataagttt atcaccaccg actatttgca
       61 acagtgccgt tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcat
      121 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
      181 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
      241 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
      301 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
      361 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
      421 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
      481 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
      541 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
      601 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
      661 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
      721 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
      781 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
      841 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
      901 cggcaaataa gatgccgctc gccagtcgat tggctgagct catgaagttc ctattccgaa
      961 gttccgcgaa cgcgtgctca ctcattaggc accgggatct cgaccgatgc ccttgagagc
     1021 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actaacatga gaattacaac
     1081 ttatatcgta tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc
     1141 tagagcggtt cagtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1201 atcttttgcc ctgtaaacga aaaaaccacc tggggaggtg gtttgatcga aggttaagtc
     1261 agttggggaa ctgcttaacc gtggtaactg gctttcgcag agcacagcaa ccaaatctgt
     1321 ccttccagtg tagccggact ttggcgcaca cttcaagagc aaccgcgtgt ttagctaaac
     1381 aaatcctctg cgaactccca gttaccaatg gctgctgcca gtggcgtttt accgtgcttt
     1441 tccgggttgg actcaagtga acagttaccg gataaggcgc agcagtcggg ctgaacgggg
     1501 agttcttgct tacagcccag cttggagcga acgacctaca ccgagccgag ataccagtgt
     1561 gtgagctatg agaaagcgcc acacttcccg taagggagaa aggcggaaca ggtatccggt
     1621 aaacggcagg gtcggaacag gagagcgcaa gagggagcga cccgccggaa acggtgggga
     1681 tctttaagtc ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc ctcacggctc
     1741 ccacagatgc accggaaaag cgtctgttta tgtgaactct ggcaggaggg cggagcctat
     1801 ggaaaaacgc caccggcgcg gccctgctgt tttgcctcac atgttagtcc cctgcttatc
     1861 cacggaatct gtgggtaact ttgtatgtgt ccgcagcgcc cgccgcagtc tcacgcccgg
     1921 agcgtagcga ccgagtgagc tagctacata tgttgacagc tagctcagtc ctaggtataa
     1981 tgctagcgga tccgaagttc ctattcttca aatagtatag gaacttcata acttcgtata
     2041 gcatacatta tacgaagtta tgaattcatt aaagaggaga aaggtaccat gcctgatata
     2101 ctcgtttgtc ttgccaatta cggagtagaa gtgccaatga atgccgaaaa atcgccggaa
     2161 aaccataacg tagaccacga agagatcgct aaatttgaag ccgtcgcctc ccgctggtgg
     2221 gatcttgaag gtgagttcaa accgttgcac cgcattaacc cgctgcgtct gggctatatt
     2281 gccgagcgtg ctggcggttt atttggcaaa aaggtgctcg atgtcggttg tggcggcggc
     2341 attctggccg agagtatggc gcgcgaaggc gcgacggtga ccggtctgga tatgggcttt
     2401 gagccattgc aggtggcaaa actgcacgcg ctggaaagcg gcattcaggt ggattatgtg
     2461 caggaaaccg tggaagagca cgcggcaaaa catgccgggc agtatgatgt ggtgacctgc
     2521 atggagatgc tggagcacgt ccccgatccg cagtcagtgg tcagagcctg tgcgcaactg
     2581 gtgaaaccag gcggcgatgt ctttttctcg acacttaacc gcaacggcaa gtcatggctg
     2641 atggcggtgg ttggtgcgga atatattttg cgcatggtgc ccaaaggcac gcatgatgtg
     2701 aagaagttta ttaaaccggc agaattgctg ggctgggtgg atcagaccag tttaaaagag
     2761 cggcatatca ctgggctgca ttacaacccg atcactaata cttttaaact cggccccggc
     2821 gtggatgtga actatatgct gcacacgcag aataagtgag agctctcaca caggacctgc
     2881 agatgacgca gccattggtc ggaaaacaga ttctcattgt tgaagatgag caggtatttc
     2941 gctcgcttct ggattcatgg ttttcctcat tgggagcgac aacggtactg gcggctgatg
     3001 gggtggatgc ccttgagttg ctgggaggtt tcactccaga cctgatgata tgtgatatcg
     3061 cgatgccacg aatgaacggg cttaaactgc tggagcatat acgtaacaga ggcgaccaga
     3121 ccccagttct ggtgatatct gccactgaaa atatggcaga tattgccaaa gcgttacgtc
     3181 tgggcgttga agatgttttg ctgaaaccag ttaaagatct gaatcgcttg cgcgagatgg
     3241 tttttgcctg tctctatccc agcatgttta attcgcgcgt tgaggaagag gaaaggcttt
     3301 ttcgcgactg ggatgcaatg gttgataacc ctgccgcagc ggcgaaatta ttacaggaac
     3361 tacaaccgcc ggttcagcag gtgatttccc attgccgggt taattatcgt caattggttg
     3421 ccgcggacaa acccggcctg gtgcttgata ttgccgcact ttcggaaaac gatctggcat
     3481 tttattgcct tgatgtcacc cgagctggac ataatggcgt acttgctgcc ttgttattac
     3541 gcgcattgtt taacggatta ttacaggaac agcttgcaca ccaaaatcaa cggttgccag
     3601 agttgggcgc gttattgaag caggtaaacc atttacttcg tcaggccaat ctgccggggc
     3661 agtttccgct attagttggc tattatcatc gcgaactgaa aaatctcatt ctggtttctg
     3721 cgggtctgaa tgcgacgtta aataccggcg aacaccaggt gcaaatcagt aatggtgttc
     3781 cgttaggcac tttaggtaac gcttatttga atcaattgag ccagcgatgc gatgcctggc
     3841 aatgccaaat atggggaacc ggtggtcgac tgcgcttgat gttgtctgca gaatgacacc
     3901 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     3961 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     4021 gttttttgct gaaaggagga actatatccg gctgcagaag cttataactt cgtatagcat
     4081 acattatacg aagttatgat atcagttcac acaggacgaa ttcatgacgc agccattggt
     4141 cggaaaacag attctcattg ttgaagatga gcaggtattt cgctcgcttc tggattcatg
     4201 gttttcctca ttgggagcga caacggtact ggcggctgat ggggtggatg cccttgagtt
     4261 gctgggaggt ttcactccag acctgatgat atgtgatatc gcgatgccac gaatgaacgg
     4321 gcttaaactg ctggagcata tacgtaacag aggcgaccag accccagttc tggtgatatc
     4381 tgccactgaa aatatggcag atattgccaa agcgttacgt ctgggcgttg aagatgtttt
     4441 gctgaaacca gttaaagatc tgaatcgctt gcgcgagatg gtttttgcct gtctctatcc
     4501 cagcatgttt aattcgcgcg ttgaggaaga ggaaaggctt tttcgcgact gggatgcaat
     4561 ggttgataac cctgccgcag cggcgaaatt attacaggaa ctacaaccgc cggttcagca
     4621 ggtgatttcc cattgccggg ttaattatcg tcaattggtt gccgcggaca aacccggcct
     4681 ggtgcttgat attgccgcac tttcggaaaa cgatctggca ttttattgcc ttgatgtcac
     4741 ccgagctgga cataatggcg tacttgctgc cttgttatta cgcgcattgt ttaacggatt
     4801 attacaggaa cagcttgcac accaaaatca acggttgcca gagttgggcg cgttattgaa
     4861 gcaggtaaac catttacttc gtcaggccaa tctgccgggg cagtttccgc tattagttgg
     4921 ctattatcat cgcgaactga aaaatctcat tctggtttct gcgggtctga atgcgacgtt
     4981 aaataccggc gaacaccagg tgcaaatcag taatggtgtt ccgttaggca ctttaggtaa
     5041 cgcttatttg aatcaattga gccagcgatg cgatgcctgg caatgccaaa tatggggaac
     5101 cggtggtcga ctgcgcttga tgttgtctgc agaatgacac caccaccacc accactgaga
     5161 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     5221 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     5281 aactatatcc ggctcgacgg atccgaagtt cctattcttc aaatagtata ggaacttcat
     5341 aacttcgtat aggatacttt atacgaagtt atgaattcat taaagaggag aaaggtacca
     5401 tgttccgtca agggatcacg ggtaggagcc accttatgag tcagaatacg ctgaaagttc
     5461 atgatttaaa tgaagatgcg gaatttgatg agaacggagt tgaggttttt gacgaaaagg
     5521 ccttagtaga agaggaaccc agtgataacg atttggccga agaggaactg ttatcgcagg
     5581 gagccacaca gcgtgtgttg gacgcgactc agctttacct tggtgagatt ggttattcac
     5641 cactgttaac ggccgaagaa gaagtttatt ttgcgcgtcg cgcactgcgt ggagatgtcg
     5701 cctctcgccg ccggatgatc gagagtaact tgcgtctggt ggtaaaaatt gcccgccgtt
     5761 atggcaatcg tggtctggcg ttgctggacc ttatcgaaga gggcaacctg gggctgatcc
     5821 gcgcggtaga gaagtttgac ccggaacgtg gtttccgctt ctcaacatac gcaacctggt
     5881 ggattcgcca gacgattgaa cgggcgatta tgaaccaaac ccgtactatt cgtttgccga
     5941 ttcacatcgt aaaggagctg aacgtttacc tgcgaaccgc acgtgagttg tcccataagc
     6001 tggaccatga accaagtgcg gaagagatcg cagagcaact ggataagcca gttgatgacg
     6061 tcagccgtat gcttcgtctt aacgagcgca ttacctcggt agacaccccg ctgggtggtg
     6121 attccgaaaa agcgttgctg gacatcctgg ccgatgaaaa agagaacggt ccggaagata
     6181 ccacgcaaga tgacgatatg aagcagagca tcgtcaaatg gctgttcgag ctgaacgcca
     6241 aacagcgtga agtgctggca cgtcgattcg gtttgctggg gtacgaagcg gcaacactgg
     6301 aagatgtagg tcgtgaaatt ggcctcaccc gtgaacgtgt tcgccagatt caggttgaag
     6361 gcctgcgccg tttgcgtgaa atcctgcaaa cgcaggggct gaatatcgaa gcgctgttcc
     6421 gcgagtaact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
     6481 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     6541 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga taagcttata
     6601 acttcgtata ggatacttta tacgaagtta tgatatcttc acacaggaaa gatcgatatg
     6661 atagtaaaag caaagtttgt aaaaggattt atcagagatg tacatcctta tggttgcaga
     6721 agggaagtac taaatcaaat agattattgt aagaaggcta ttgggtttag gggaccaaag
     6781 aaggttttaa ttgttggagc ctcatctggg tttggtcttg ctactagaat ttcagttgca
     6841 tttggaggtc cagaagctca cacaattgga gtatcctatg aaacaggagc tacagataga
     6901 agaataggaa cagcgggatg gtataataac atatttttta aagaatttgc taaaaaaaaa
     6961 ggattagttg caaaaaactt cattgaggat gccttttcta atgaaaccaa agataaagtt
     7021 attaagtata taaaggatga atttggtaaa atagatttat ttgtttatag tttagctgcg
     7081 cctaggagaa aggactataa aactggaaat gtttatactt caagaataaa aacaatttta
     7141 ggagattttg agggaccgac tattgatgtt gaaagagacg agattacttt aaaaaaggtt
     7201 agtagtgcta gcattgaaga aattgaagaa actagaaagg taatgggtgg agaggattgg
     7261 caagagtggt gtgaagagct gctttatgaa gattgttttt cggataaagc aactaccata
     7321 gcatactcgt atataggatc cccaagaacc tacaagatat atagagaagg tactatagga
     7381 atagctaaaa aggatcttga agataaggct aagcttataa atgaaaaact taacagagtt
     7441 ataggtggta gagcctttgt gtctgtgaat aaagcattag ttacaaaagc aagtgcatat
     7501 attccaactt ttcctcttta tgcagctatt ttatataagg tcatgaaaga aaaaaatatt
     7561 catgaaaatt gtattatgca aattgagaga atgttttctg aaaaaatata ttcaaatgaa
     7621 aaaatacaat ttgatgacaa gggaagatta aggatggacg atttagagct tagaaaagac
     7681 gttcaagacg aagttgatag aatatggagt aatattactc ctgaaaattt taaggaatta
     7741 tctgattata agggatacaa aaaagaattc atgaacttaa acggttttga tctagatggg
     7801 gttgattata gtaaagacct ggatatagaa ttattaagaa aattagaacc ttaagaattc
     7861 tagcaggagg aagattccat gaaaaaggta tgtgttatag gtgcaggtac tatgggttca
     7921 ggaattgctc aggcatttgc agctaaagga tttgaagtag tattaagaga tattaaagat
     7981 gaatttgttg atagaggatt agattttatc aataaaaatc tttctaaatt agttaaaaaa
     8041 ggaaagatag aagaagctac taaagttgaa atcttaacta gaatttccgg aacagttgac
     8101 cttaatatgg cagctgattg cgatttagtt atagaagcag ctgttgaaag aatggatatt
     8161 aaaaagcaga tttttgctga cttagacaat atatgcaagc cagaaacaat tcttgcatca
     8221 aatacatcat cactttcaat aacagaagtg gcatcagcaa ctaaaactaa tgataaggtt
     8281 ataggtatgc atttctttaa tccagctcct gttatgaagc ttgtagaggt aataagagga
     8341 atagctacat cacaagaaac ttttgatgca gttaaagaga catctatagc aataggaaaa
     8401 gatcctgtag aagtagcaga agcaccagga tttgttgtaa atagaatatt aataccaatg
     8461 attaatgaag cagttggtat attagcagaa ggaatagctt cagtagaaga catagataaa
     8521 gctatgaaac ttggagctaa tcacccaatg ggaccattag aattaggtga ttttataggt
     8581 cttgatatat gtcttgctat aatggatgtt ttatactcag aaactggaga ttctaagtat
     8641 agaccacata cattacttaa gaagtatgta agagcaggat ggcttggaag aaaatcagga
     8701 aaaggtttct acgattattc aaaataaaag cttattaaag aggagaaagg taccatggaa
     8761 ctaaacaatg tcatccttga aaaggaaggt aaagttgctg tagttaccat taacagacct
     8821 aaagcattaa atgcgttaaa tagtgataca ctaaaagaaa tggattatgt tataggtgaa
     8881 attgaaaatg atagcgaagt acttgcagta attttaactg gagcaggaga aaaatcattt
     8941 gtagcaggag cagatatttc tgagatgaag gaaatgaata ccattgaagg tagaaaattc
     9001 gggatacttg gaaataaagt gtttagaaga ttagaacttc ttgaaaagcc tgtaatagca
     9061 gctgttaatg gttttgcttt aggaggcgga tgcgaaatag ctatgtcttg tgatataaga
     9121 atagcttcaa gcaacgcaag atttggtcaa ccagaagtag gtctcggaat aacacctggt
     9181 tttggtggta cacaaagact ttcaagatta gttggaatgg gcatggcaaa gcagcttata
     9241 tttactgcac aaaatataaa ggcagatgaa gcattaagaa tcggacttgt aaataaggta
     9301 gtagaaccta gtgaattaat gaatacagca aaagaaattg caaacaaaat tgtgagcaat
     9361 gctccagtag ctgttaagtt aagcaaacag gctattaata gaggaatgca gtgtgatatt
     9421 gatactgctt tagcatttga atcagaagca tttggagaat gcttttcaac agaggatcaa
     9481 aaggatgcaa tgacagcttt catagagaaa agaaaaattg aaggcttcaa aaatagatag
     9541 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
     9601 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     9661 gtcttgaggg gttttttgct gaaaggagga actatatccg gaagatctat taccctgtta
     9721 tcccta
//
