LOCUS       40924_10741        5435 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCint2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Zhang JY, Lin GM, Xing WY, Zhang CC.
  TITLE     Diversity of Growth Patterns Probed in Live Cyanobacterial Cells 
            Using a Fluorescent Analog of a Peptidoglycan Precursor
  JOURNAL   Front Microbiol 9, 791 (2018)
  PUBMED    29740419
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Zhang J-Y., Lin G-M., Xing W-Y., Zhang C-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2018) Institute of Hydrobiology, Donghu Nanlu, 
            Wuhan, Hubei 430072, 086
REFERENCE   3  (bases 1 to 5435)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5435)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol 9, 791 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-2018) Institute of Hydrobiology, Donghu Nanlu, Wuhan, Hubei 
            430072, 086"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(476..1894)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLEVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(1895..2340)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     promoter        2488..2590
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2591..3247
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      4380..4968
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(5154..5294)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 gctggctgtt ttacgcgtat gacaggctcc ggaagacggt tgttgcgcac gtattcggtg
       61 aacgcactat ggcgacgctg gggcgtctta tgagcctgct gtcacccttt gacgtggtga
      121 tatggatgac ggatggctgg ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa
      181 tcagcaagcg atatacgcag cgaattgagc ggcataacct gaatctgagg cagcacctgg
      241 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
      301 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc aaaaggttag
      361 gaatacggtt agccatttgc ctgcttttat atagttcata tgggattcac ctttatgttg
      421 ataagaaata aaagaaaatg ccaataggat atcggcattt tcttttgcgt ttttatttgt
      481 taactgttaa ttgtccttgt tcaaggatgc tgtctttgac aacagatgtt ttcttgcctt
      541 tgatgttcag caggaagctt ggcgcaaacg ttgattgttt gtctgcgtag aatcctctgt
      601 ttgtcatata gcttgtaatc acgacattgt ttcctttcgc ttgaggtaca gcgaagtgtg
      661 agtaagtaaa ggttacatcg ttaggatcaa gatccatttt taacacaagg ccagttttgt
      721 tcagcggctt gtatgggcca gttaaagaat tagaaacata accaagcatg taaatatcgt
      781 tagacgtaat gccgtcaatc gtcatttttg atccgcggga gtcagtgaac aggtaccatt
      841 tgccgttcat tttaaagacg ttcgcgcgtt caatttcatc tgttactgtg ttagatgcaa
      901 tcagcggttt catcactttt ttcagtgtgt aatcatcgtt tagctcaatc ataccgagag
      961 cgccgtttgc taactcagcc gtgcgttttt tatcgctttg cagaagtttt tgactttctt
     1021 gacggaagaa tgatgtgctt ttgccatagt atgctttgtt aaataaagat tcttcgcctt
     1081 ggtagccatc ttcagttcca gtgtttgctt caaatactaa gtatttgtgg cctttatctt
     1141 ctacgtagtg aggatctctc agcgtatggt tgtcgcctga gctgtagttg ccttcatcga
     1201 tgaactgctg tacattttga tacgtttttc cgtcaccgtc aaagattgat ttataatcct
     1261 ctacaccgtt gatgttcaaa gagctgtctg atgctgatac gttaacttgt gcagttgtca
     1321 gtgtttgttt gccgtaatgt ttaccggaga aatcagtgta gaataaacgg atttttccgt
     1381 cagatgtaaa tgtggctgaa cctgaccatt cttgtgtttg gtcttttagg atagaatcat
     1441 ttgcatcgaa tttgtcgctg tctttaaaga cgcggccagc gtttttccag ctgtcaatag
     1501 aagtttcgcc gactttttga tagaacatgt aaatcgatgt gtcatccgca tttttaggat
     1561 ctccggctaa tgcaaagacg atgtggtagc cgtgatagtt tgcgacagtg ccgtcagcgt
     1621 tttgtaatgg ccagctgtcc caaacctcca ggccttttgc agaagagata tttttaattg
     1681 tggacgaatc gaattcagga acttgatatt tttcattttt ttgctgttca gggatttgca
     1741 gcatatcatg gcgtgtaata tgggaaatgc cgtatgtttc cttatatggc ttttggttcg
     1801 tttctttcgc aaacgcttga gttgcgcctc ctgccagcag tgcggtagta aaggttaata
     1861 ctgttgcttg ttttgcaaac tttttgatgt tcatcgttca tgtctccttt tttatgtact
     1921 gtgttagcgg tctgcttctt ccagccctcc tgtttgaaga tggcaagtta gttacgcaca
     1981 ataaaaaaag acctaaaata tgtaaggggt gacgccaaag tatacacttt gccctttaca
     2041 cattttaggt cttgcctgct ttatcagtaa caaacccgcg cgatttactt ttcgacctca
     2101 ttctattaga ctctcgtttg gattgcaact ggtctatttt cctcttttgt ttgatagaaa
     2161 atcataaaag gatttgcaga ctacgggcct aaagaactaa aaaatctatc tgtttctttt
     2221 cattctctgt attttttata gtttctgttg catgggcata aagttgcctt tttaatcaca
     2281 attcagaaaa tatcataata tctcatttca ctaaataata gtgaacggca ggtatatgtg
     2341 atgggttaaa aaggatcgat cctctagcta gagtcgactc atcgaataaa tacctgtgac
     2401 ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg
     2461 ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca
     2521 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     2581 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     2641 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     2701 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     2761 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     2821 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     2881 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     2941 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     3001 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     3061 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     3121 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     3181 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     3241 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga
     3301 ataagtgata ataagcggat gaatggcaga aattcgatat ctagatctcg agctggatac
     3361 ttcccgtccg ccagggggac atgccggcga tgctgaaggt cgcgcgcatt cccgatgaag
     3421 aggccggtta ccgcctgttt gaggatatag taatctttct aaatagcttt ggattggagg
     3481 agtatggcca ctaatactaa gttcagctaa taaaaaaatt tgctaaagaa ctccagctgg
     3541 atttcactga tgagaatatc gtcggagata aatataataa ttccacggac tatagactat
     3601 actagtatac tccgtctact gtacgataca cttccgctca ggtccttgtc ctttaacgag
     3661 gattgttacg tacgctaatg gcgtcaaaac aaagactcta gacctaggcc ttaagatcga
     3721 aagagctggt agttggcgca ctgttcgaag aactgccgat gtccagtaag attcttacta
     3781 tgctggttga accggatgct ggtaaagcta cttgggttgc tgcttctact tatggtaccg
     3841 atacaactac tggtgaggaa gttaaaggag ctcgcgaaag cttgcatgcc tgcagcaatg
     3901 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3961 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4021 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4081 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4141 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4201 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4261 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4321 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4381 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4441 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4501 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     4561 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4621 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4681 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4741 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     4801 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4861 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4921 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4981 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5041 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5101 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     5161 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     5221 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     5281 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     5341 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     5401 ggttttcacc gtcatcaccg aaacgcgcga ggcag
//
