LOCUS       40924_10596        9118 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             59..1558
                     /codon_start=1
                     /label=HIV-1 gag
                     /note="gag protein from human immunodeficiency virus 1"
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             1354..4362
                     /codon_start=1
                     /label=HIV-1 pol
                     /note="pol protein from human immunodeficiency virus 1"
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVGQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4429..4662
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     polyA_signal    complement(5161..5282)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5463..5918
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6252..6356
                     /label=AmpR promoter
     CDS             6357..7214
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7388..7976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        8188..8566
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8567..8770
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          8906..9038
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        9083..9101
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaggatcc gcggatccat
       61 gggcgcccgc gccagcgtgc tgtccggcgg cgagctggat aaatgggaaa aaattcggtt
      121 aaggccaggg ggaaagaaac aatataaact aaaacatata gtatgggcaa gcagggagct
      181 agaacgattc gcagttaatc ctggcctttt agagacatca gaaggctgta gacaaatact
      241 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
      301 aatagcagtc ctctattgtg tgcatcaaag gatagatgta aaagacacca aggaagcctt
      361 agataagata gaggaagagc aaaacaaaag taagaaaaag gcacagcaag cagcagctga
      421 cacaggaaac aacagccagg tcagccaaaa ttaccctata gtgcagaacc tccaggggca
      481 aatggtacat caggccatat cacctagaac tttaaatgca tgggtaaaag tagtagaaga
      541 gaaggctttc agcccagaag taatacccat gttttcagca ttatcagaag gagccacccc
      601 acaagattta aataccatgc taaacacagt ggggggacat caagcagcca tgcaaatgtt
      661 aaaagagacc atcaatgagg aagctgcaga atgggataga ttgcatccag tgcatgcagg
      721 gcctattgca ccaggccaga tgagagaacc aaggggaagt gacatagcag gaactactag
      781 tacccttcag gaacaaatag gatggatgac acataatcca cctatcccag taggagaaat
      841 ctataaaaga tggataatcc tgggattaaa taaaatagta agaatgtata gccctaccag
      901 cattctggac ataagacaag gaccaaagga accctttaga gactatgtag accgattcta
      961 taaaactcta agagccgagc aagcttcaca agaggtaaaa aattggatga cagaaacctt
     1021 gttggtccaa aatgcgaacc cagattgtaa gactatttta aaagcattgg gaccaggagc
     1081 gacactagaa gaaatgatga cagcatgtca gggagtgggg ggacccggcc ataaagcaag
     1141 agttttggct gaagcaatga gccaagtaac aaatccagct accataatga tacagaaagg
     1201 caattttagg aaccaaagaa agactgttaa gtgtttcaat tgtggcaaag aagggcacat
     1261 agccaaaaat tgcagggccc ctaggaaaaa gggctgttgg aaatgtggaa aggaaggaca
     1321 ccaaatgaaa gattgtactg agagacaggc taatttttta gggaagatct ggccttccca
     1381 caagggaagg ccagggaatt ttcttcagag cagaccagag ccaacagccc caccagaaga
     1441 gagcttcagg tttggggaag agacaacaac tccctctcag aagcaggagc cgatagacaa
     1501 ggaactgtat cctttagctt ccctcagatc actctttggc agcgacccct cgtcacaata
     1561 aagatagggg ggcaattaaa ggaagctcta ttagatacag gagcagatga tacagtatta
     1621 gaagaaatga atttgccagg aagatggaaa ccaaaaatga tagggggaat tggaggtttt
     1681 atcaaagtag gacagtatga tcagatactc atagaaatct gcggacataa agctataggt
     1741 acagtattag taggacctac acctgtcaac ataattggaa gaaatctgtt gactcagatt
     1801 ggctgcactt taaattttcc cattagtcct attgagactg taccagtaaa attaaagcca
     1861 ggaatggatg gcccaaaagt taaacaatgg ccattgacag aagaaaaaat aaaagcatta
     1921 gtagaaattt gtacagaaat ggaaaaggaa ggaaaaattt caaaaattgg gcctgaaaat
     1981 ccatacaata ctccagtatt tgccataaag aaaaaagaca gtactaaatg gagaaaatta
     2041 gtagatttca gagaacttaa taagagaact caagatttct gggaagttca attaggaata
     2101 ccacatcctg cagggttaaa acagaaaaaa tcagtaacag tactggatgt gggcgatgca
     2161 tatttttcag ttcccttaga taaagacttc aggaagtata ctgcatttac catacctagt
     2221 ataaacaatg agacaccagg gattagatat cagtacaatg tgcttccaca gggatggaaa
     2281 ggatcaccag caatattcca gtgtagcatg acaaaaatct tagagccttt tagaaaacaa
     2341 aatccagaca tagtcatcta tcaatacatg gatgatttgt atgtaggatc tgacttagaa
     2401 atagggcagc atagaacaaa aatagaggaa ctgagacaac atctgttgag gtggggattt
     2461 accacaccag acaaaaaaca tcagaaagaa cctccattcc tttggatggg ttatgaactc
     2521 catcctgata aatggacagt acagcctata gtgctgccag aaaaggacag ctggactgtc
     2581 aatgacatac agaaattagt gggaaaattg aattgggcaa gtcagattta tgcagggatt
     2641 aaagtaaggc aattatgtaa acttcttagg ggaaccaaag cactaacaga agtagtacca
     2701 ctaacagaag aagcagagct agaactggca gaaaacaggg agattctaaa agaaccggta
     2761 catggagtgt attatgaccc atcaaaagac ttaatagcag aaatacagaa gcaggggcaa
     2821 ggccaatgga catatcaaat ttatcaagag ccatttaaaa atctgaaaac aggaaaatat
     2881 gcaagaatga agggtgccca cactaatgat gtgaaacaat taacagaggc agtacaaaaa
     2941 atagccacag aaagcatagt aatatgggga aagactccta aatttaaatt acccatacaa
     3001 aaggaaacat gggaagcatg gtggacagag tattggcaag ccacctggat tcctgagtgg
     3061 gagtttgtca atacccctcc cttagtgaag ttatggtacc agttagagaa agaacccata
     3121 ataggagcag aaactttcta tgtagatggg gcagccaata gggaaactaa attaggaaaa
     3181 gcaggatatg taactgacag aggaagacaa aaagttgtcc ccctaacgga cacaacaaat
     3241 cagaagactg agttacaagc aattcatcta gctttgcagg attcgggatt agaagtaaac
     3301 atagtgacag actcacaata tgcattggga atcattcaag cacaaccaga taagagtgaa
     3361 tcagagttag tcagtcaaat aatagagcag ttaataaaaa aggaaaaagt ctacctggca
     3421 tgggtaccag cacacaaagg aattggagga aatgaacaag tagataaatt ggtcagtgct
     3481 ggaatcagga aagtactatt tttagatgga atagataagg cccaagaaga acatgagaaa
     3541 tatcacagta attggagagc aatggctagt gattttaacc taccacctgt agtagcaaaa
     3601 gaaatagtag ccagctgtga taaatgtcag ctaaaagggg aagccatgca tggacaagta
     3661 gactgtagcc caggaatatg gcagctagat tgtacacatt tagaaggaaa agttatcttg
     3721 gtagcagttc atgtagccag tggatatata gaagcagaag taattccagc agagacaggg
     3781 caagaaacag catacttcct cttaaaatta gcaggaagat ggccagtaaa aacagtacat
     3841 acagacaatg gcagcaattt caccagtact acagttaagg ccgcctgttg gtgggcgggg
     3901 atcaagcagg aatttggcat tccctacaat ccccaaagtc aaggagtaat agaatctatg
     3961 aataaagaat taaagaaaat tataggacag gtaagagatc aggctgaaca tcttaagaca
     4021 gcagtacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     4081 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4141 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     4201 tggaaaggac cagcaaagct cctctggaaa ggtgaagggg cagtagtaat acaagataat
     4261 agtgacataa aagtagtgcc aagaagaaaa gcaaagatca tcagggatta tggaaaacag
     4321 atggcaggtg atgattgtgt ggcaagtaga caggatgagg attaacaccc atagaatggc
     4381 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     4441 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     4501 acaggccaga caattattgt ctgatatagt gcagcagcag aacaatttgc tgagggctat
     4561 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaaacagc tccaggcaag
     4621 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     4681 tggaaaactc atttgcacca ctgctgtgcc gcggccgcaa aaaaggggct cgtccctgtt
     4741 tccggaggaa tttgcaagcg gggtcttgca tgacggggag gcaaaccccc gttcggccgc
     4801 agtccggccg gcccgagact cgaaccgggg gtcctgcgac tcaacccttg gaaaataacc
     4861 ctccggctac agggagcgag ccacttaatg ctttcgcttt ccagcctaac cgcttacgcc
     4921 gcgcgcgcca gtggccaaaa aagctagcgc agcagccgcc gcgcctggaa ggaagccaaa
     4981 aggagcgctc ccccgttgtc tgacgtcgca cacctgggtt cgacacgcgg gcggtaaccg
     5041 catggatcac ggcggacggc cggatccggg gttcgaaccc cggtcgtccg ccatgatacc
     5101 cttgcgaatt tatccaccag accacggaag agtgcccgcg gccgcttcga gcagacatga
     5161 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     5221 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     5281 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
     5341 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg ggctggcgta
     5401 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5461 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5521 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5581 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5641 tagagcttta cggcacctcg accgcaaaaa acttgatttg ggtgatggtt cacgtagtgg
     5701 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5761 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5821 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaatatt
     5881 taacgcgaat tttaacaaaa tattaacgtt tacaatttcg cctgatgcgg tattttctcc
     5941 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     6001 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     6061 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6121 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     6181 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     6241 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6301 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6361 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6421 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6481 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6541 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6601 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6661 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6721 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6781 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6841 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6901 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6961 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7021 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7081 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7141 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7201 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7261 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7321 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7381 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7441 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7501 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     7561 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7621 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7681 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7741 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7801 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7861 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7921 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7981 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatggctc
     8041 gacagatctt caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     8101 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     8161 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     8221 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     8281 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     8341 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     8401 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
     8461 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
     8521 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     8581 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     8641 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     8701 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     8761 aagcagagct cgtttagtga accgtcagat cactagaagc tttattgcgg tagtttatca
     8821 cagttaaatt gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag
     8881 aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga
     8941 ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat
     9001 tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta
     9061 cagctcttaa ggctagagta cttaatacga ctcactatag gctagcctcg agaattca
//
