LOCUS       40924_10561        8978 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCgeA-CL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T, 
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8978)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T, 
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology, 
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 8978)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8978)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical 
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advance v. 10
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        106..210
                     /label=AmpR promoter
     CDS             211..1068
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1242..1830
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2016..2156)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2261..2449)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(3703..4719)
                     /codon_start=1
                     /gene="amyE"
                     /product="AmyE"
                     /label=amyE
                     /note="alpha-amylase"
                     /protein_id="AHG54582.1"
                     /translation="MSDDDIRLGWAVIASRSGSTPLFFSRPEGGGNGVRFPGKSQIGDR
                     GSALFEDQAITAVNRFHNVMAGQPEELSNPNGNNQIFMNQRGSHGVVLANAGSSSVSIN
                     TATKLPDGRYDNKAGAGSFQVNDGKLTGTINARSVAVLYPDDIAKAPHVFLENYKTGVT
                     HSFNDQLTITLRADANTTKAVYQINNGPETAFKDGDQFTIGKGDPFGKTYTIMLKGTNS
                     DGVTRTEKYSFVKRDPASAKTIGYQNPNHWSQVNAYIYKHDGSRVIELTGSWPGKPMTK
                     NADGIYTLTLPADTDTTNAKVIFNNGSAQVPGQNQPGFDYVLNGLYNDSGLSGSLPH"
     gene            complement(3703..4719)
                     /gene="amyE"
                     /label=amyE
     CDS             complement(4829..5581)
                     /codon_start=1
                     /gene="trpC"
                     /product="TrpC"
                     /label=trpC
                     /protein_id="AHG54581.1"
                     /translation="MLEKIIKQKKEEVKTLVLPVEQPFEKRSFKEAPASPNRFIGLIAE
                     VKKASPSKGLIKEDFVPVQIAKDYEAAKADAISVLTDTPFFQGENSYLSDVKRAVSIPV
                     LRKDFIIDSLQVEESRRIGADAILLIGEVLDPLHLHELYLEAGEKGMDVLVEVHDASTL
                     EQILKVFTPDILGVNNRNLKTFETSVKQTEQIASLVPKESLLVSESGIGSLEHLTFVNE
                     HGARAVLIGESLMRQTSQRKAIHALFRE"
     gene            complement(4829..5581)
                     /gene="trpC"
                     /label=trpC
     regulatory      complement(5587..5596)
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(5609..5637)
                     /regulatory_class="promoter"
     CDS             6658..7107
                     /codon_start=1
                     /gene="cgeA"
                     /product="CgeA"
                     /label=cgeA
                     /note="spore coat protein"
                     /protein_id="AHG54580.1"
                     /translation="MSSENAQLKKDLIKAVLSPLFPTATEGGENMDSNLKALLDAAIDQ
                     KVDESETVTAESILDPSLPARWIFARITPGTTISIVTDSGDMIGPVVFVAFDQVHGIVF
                     VTQESSVTPAGQATTLIDVDKVESVTFFSGGGEAAAKGGGGSLRR"
     gene            6658..7107
                     /gene="cgeA"
                     /label=cgeA
     gene            complement(8311..8864)
                     /gene="amyE"
                     /label=amyE
     CDS             complement(8322..8864)
                     /codon_start=1
                     /gene="amyE"
                     /product="alpha-amylase"
                     /label=amyE
                     /protein_id="AHG54579.1"
                     /translation="MFAKRFKTSLLPLFAGFLLLFHLVLAGPAAASAETANKSNELTAP
                     SIKSGTILHAWNWSFNTLKHNMKDIHDAGYTAIQTSPINQVKEGNQGDKSMSNWYWLYQ
                     PTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAVINHTTSDYAAISNEVKSIPNWTH
                     GNTQIKNWSDRWDVTQN"
ORIGIN
        1 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      421 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
      481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
      661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
      721 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
      781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
      841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
      901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
      961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     2041 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gactaagaaa atgccgtcaa atccgctcgc
     3421 catgacttca ctaacgatgc ctttgaaaat cttcaagttc ttttctacta attcaaggcg
     3481 tgtctcacca ggtttttggt ttgctccggc gcaaatgcag acaatatcag catccttgca
     3541 gggtatgttt ctctttgatg tctttttgtt tgtgaagtat ttcacattta tattgtgcaa
     3601 cacttcacaa acttttgcaa gagaaaagtt ttgtctgatt tatgaacaaa aaagaaacca
     3661 tcattgatgg tttctttcgg taagtcccgt ctagccttgc cctcaatggg gaagagaacc
     3721 gcttaagccc gagtcattat ataaaccatt tagcacgtaa tcaaagccag gctgattctg
     3781 accgggcact tgggcgctgc cattattaaa aatcactttt gcgttggttg tatccgtgtc
     3841 cgcaggcagc gtcagcgtgt aaattccgtc tgcattttta gtcattggtt ttccaggcca
     3901 agatccggtc aattcaatta ctcggctccc atcatgttta tagatataag catttacctg
     3961 gctccaatga ttcggatttt gatagccgat ggttttggcc gacgctggat ctcttttaac
     4021 aaaactgtat ttctcggtcc tcgttacacc atcactgttc gttcctttta acatgatggt
     4081 gtatgttttg ccaaatggat ctccttttcc gattgtgaat tgatctccat ccttaaacgc
     4141 cgtctctggt ccattattga tttgataaac ggcttttgtt gtattcgcat ctgcacgcaa
     4201 ggtaatcgtc agttgatcat tgaaagaatg tgttacacct gttttgtaat tctcaaggaa
     4261 aacatgaggc gcttttgcaa tatcatcagg ataaagcaca gctacagacc tggcattgat
     4321 cgtgcctgtc agtttaccat cgttcacttg aaatgaaccc gctccagctt tattgtcata
     4381 cctgccatca ggcaattttg ttgccgtatt gatagagaca gaggatgaac ctgcatttgc
     4441 cagcacaacg ccatgtgagc cgcgctgatt cataaatatc tggttgtttc cattcgggtt
     4501 cgagagttcc tcaggctgtc cagccatcac attgtgaaat ctattgaccg cagtgatagc
     4561 ctgatcttca aataaagcac tcccgcgatc gcctatttgg cttttccccg ggaacctcac
     4621 accatttccg cctccctcag gtctggaaaa gaaaagaggc gtactgcctg aacgagaagc
     4681 tatcaccgcc cagcctaaac ggatatcatc atcgctcatc catgtcgacg ctctccctta
     4741 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
     4801 gcaaggaatg gtgcatgcaa agtacgtatt actccctaaa caaagcatgg attgctttac
     4861 gctgagaagt ttgtctcatc aatgattcac cgataagtac agctcgcgcc ccatgttcat
     4921 tgacaaatgt taaatgttct aaagaaccga ttccgctttc gctgacaagc aaggattctt
     4981 tcggaacgag agatgcgatt tgttctgtct gctttacaga tgtttcaaac gtttttaggt
     5041 ttcgattatt tacgccgaga atgtcgggtg tgaacacttt caatatttgt tctagcgttg
     5101 atgcatcatg aacctccact aacacgtcca tccccttttc acctgcttca agatataatt
     5161 catgaaggtg taagggatca agcacctcgc cgattaacaa tatggcatcc gctccgattc
     5221 ttcttgattc ctctacttga agagaatcaa taataaaatc ttttctaagt acaggaatcg
     5281 aaacagcacg ctttacgtct gataaatagc tgttttcccc ttgaaaaaac ggggtgtctg
     5341 ttaaaacgga aatcgcatct gccttcgcag cctcatagtc ttttgcaatc tgcacaggta
     5401 caaaatcctc tttaataagc ccttttgacg gcgatgcttt cttcacttcg gcaatcaacc
     5461 cgataaaccg attcgggctt gccggcgcct ccttaaatga acgtttctcg aaaggctgct
     5521 ctaccggcag aaccagtgtt ttcacttctt ctttcttttg tttgatgatt ttttcaagca
     5581 tgggtctcac ctccttggcg actacttaat agtaacattt ttagttaact aggtcaatac
     5641 ttttttgaaa agaattcttg agagtgaaac atgagatctc gtgttattta acgggttttg
     5701 gaaaataacc aaatcagtca gaacttcttt tacgatgaca ttttgaagtg tatctccgtt
     5761 gatcaaattg acttttgaaa caattgtcta aatcaatttt attaaagttc atttgatatg
     5821 cctcctaaat ttttatctaa agtgaattta ggaggcttac ttgtctgctt tcttcattag
     5881 aatcaatcct tttttaaaag tcaatattac tgtaacataa atatatattt taaaaatatc
     5941 ccactttatc caattttcgt ttgttgaact aatgggtgct ttagttgaag aataaaagac
     6001 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
     6061 ttttctttct tatcttgata ataagggtaa ctattgccga tgataagctg tcaaacatga
     6121 gaattcaagc agagcctctg tcatcattta aaaagcaccc cagcttacaa cacttgagag
     6181 tgaaacatga gatctcgtgt tatttaacgg gttttggaaa ataaccaaat cagtcagaac
     6241 ttcttttacg atgacatttt gaagtgtatc tccgttgatc aaattgactt ttgaaaccgg
     6301 tacgcccagc tcttttgcaa aaacaagctg gcgtctgagg tcagatggat ttccttcaga
     6361 agacgattga cgtatcagca gaatcagcaa aagcaaaata aaatgcagat catcttggtt
     6421 ttcttctcga tccacttgtt tctctaaatc caaatcatcc ctctccctta tccttatctc
     6481 tgtttcacaa tatgtgcgac ttcgatttat ggtataggct atgtccactc cattaaaaaa
     6541 tcagcatttt ccttaaatag atgaaaaata gatcaggtac ggcgttcgac tcataccaaa
     6601 taacagccgg aagaatatga ataacgtgag ttcactatcg aataggaggt gtgtgtaatg
     6661 agctctgaaa atgcacagtt aaaaaaggat ttaattaaag ccgttttaag ccctttattt
     6721 ccaactgcaa cagaaggagg agaaaatatg gatagtaatc ttaaagcctt gcttgatgct
     6781 gccatcgatc aaaaagtaga tgaaagtgaa acggttacgg cagaatctat tttagaccct
     6841 tctcttccgg caagatggat ttttgccaga attacgccag gcactacaat cagcattgtg
     6901 actgattcag gtgacatgat cggaccggtt gttttcgttg ctttcgatca ggttcacggg
     6961 atcgtatttg taacacagga aagctccgtc actccggcag gccaagctac aacattaatt
     7021 gatgtagaca aagtagaaag cgttacgttc ttttcaggtg gaggagaagc agcagcgaaa
     7081 ggtggaggag gatccttacg taggtaaccg ttaactctag atttgacgtg ggtaccgagc
     7141 tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg
     7201 atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg
     7261 ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag
     7321 ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc
     7381 ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc
     7441 tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg
     7501 gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata
     7561 acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga
     7621 aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg
     7681 ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta
     7741 tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg
     7801 aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg
     7861 tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta
     7921 ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct
     7981 tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt
     8041 ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca
     8101 gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata
     8161 tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaatta cagctgagcg
     8221 ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg ggcaggccat
     8281 gtctgcccgt atttcgcgta aggaaatcca ttatgtacta tttaattctg cgtgacatcc
     8341 catcgatcag accagttttt aatttgtgtg tttccatgtg tccagtttgg aatactctta
     8401 acctcattgg aaatcgcggc ataatcactg gtggtatgat tgatgaccgc gtcaacaatg
     8461 acctttatgc catattcttc agcggctgca cacatttctt taaattcttg ttcagtacct
     8521 aagtaacggt tgccaatttg atacgatgtc ggctgataca gccagtacca gttcgacatg
     8581 cttttatctc cttgattccc ttcctttact tggttaatcg gagatgtctg aatggctgta
     8641 tatcctgcat catgaatatc cttcatattg tgttttaacg tattgaacga ccaattccat
     8701 gcatgaagaa tggttccgct tttgatcgac ggtgctgtaa gctcattcga tttgttcgcc
     8761 gtttcagcac tcgcagccgc cggtcctgcc agaaccaaat gaaacagcaa taaaaatcca
     8821 gcgaataacg gcagtaaaga ggttttgaat cgttttgcaa acattcttga cactccttat
     8881 ttgatttttt gaagacttac ttcggagtca aaaatccctc ttacttcatt cttccgcttc
     8941 ctcctttcaa accgatgtga agactggaga attttgtt
//
