LOCUS       V008545                 7306 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008545
VERSION     V008545
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 7306)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7306)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-NOV-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            247..879
                     /gene="thrC"
                     /label="thrC"
     CDS             247..864
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label="thrC"
                     /note="N terminal part of thrC"
                     /protein_id="AHG54645.1"
                     /translation="MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIEL
                     HVKTEGVNPTGSFKDRGMVMAVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVI
                     IPNGKIAFGKLAQAVMYGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIEGQK
                     TAAFEIVHNGFPYAKYGQTWPARLLLFLTPDQLVMVATGAQL"
     gene            complement(1133..1643)
                     /gene="cgeA"
                     /label="cgeA"
     CDS             complement(1133..1579)
                     /codon_start=1
                     /gene="cgeA"
                     /product="CpgA"
                     /label="cgeA"
                     /note="spore coat protein"
                     /protein_id="AHG54642.1"
                     /translation="MSSENAQLKKDLIKAVLSPLFPTATEGGENMDSNLKALLDAAIDQ
                     KVDESETVTAESILDPSLPARWIFARITPGTTISIVTDSGDMIGPVVFVAFDQVHGIVF
                     VTQESSVTPAGQATTLIDVDKVESVTFFSGGGEAAAKGGGGSGR"
     regulatory      complement(1588..1593)
                     /gene="cgeA"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(1615..1623)
                     /gene="cgeA"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(1640..1643)
                     /gene="cgeA"
                     /regulatory_class="minus_35_signal"
     CDS             2187..2939
                     /codon_start=1
                     /gene="trpC"
                     /product="TrpC"
                     /label="trpC"
                     /protein_id="AHG54646.1"
                     /translation="MLEKIIKQKKEEVKTLVLPVEQPFEKRSFKEALASPNRFIGLIAE
                     VKKASPSKGLIKEDFVPVQIAKDYEAAKADAISVLTDTPFFQGENSYLSDVKRAVSIPV
                     LRKDFIIDSLQVEESRRIGADAILLIGEVLDPLHLHELYLEAGEKGMDVLVEVHDASTL
                     EQILKVFTPDILGVNNRNLKTFETSVKQTEQIASLVPKESLLVSESGIGSLEHLTFVNE
                     HGARAVLIGESLMRQTSQRKAIHALFRE"
     gene            2187..2939
                     /gene="trpC"
                     /label="trpC"
     CDS             3369..3797
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label="thrC"
                     /note="C-terminal part of thrC"
                     /protein_id="AHG54644.1"
                     /translation="MRGFEAEGAAAIVRNEVIENPETIATAIRIGNPASWDKAVKAAEE
                     SNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVKSGEIPKGSKVVAVLT
                     GNGLKDPNTAVDISEIKPVTLPTDEDSILEYVKGAARV"
     gene            3369..3797
                     /gene="thrC"
                     /label="thrC"
     CDS             3794..4204
                     /codon_start=1
                     /gene="thrB"
                     /product="ThrB"
                     /label="thrB"
                     /note="N terminal part of thrB"
                     /protein_id="AHG54643.1"
                     /translation="MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWS
                     FEAETETVAGINSSSFNFLTSSCIGTISRSSNPSSFTSIRFNQHSKNLTGHRQFFEQCA
                     NYQLFLQFIQGTGEPACNPSCSIMRNDPHPVS"
     gene            3794..4204
                     /gene="thrB"
                     /label="thrB"
     CDS             4448..5227
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     rep_origin      complement(5477..6065)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6239..7096)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7097..7201)
                     /label="AmpR promoter"
ORIGIN
        1 aattcatgta aaagatgagg ttggttcatt ctcgaaaatt acatctgtgt tctcagagcg
       61 gggcgtgagc tttgaaaaaa tccttcagct gccaattaaa ggccatgatg agttagctga
      121 aatcgtaatt gtcacacatc atacatcaga agctgatttc agtgatatcc tgcaaaacct
      181 aaatgatttg gaagtcgttc aagaagtcaa aagcacatat cgtgtagaag ggaacggttg
      241 gagctaatgt ggaaaggact tatccatcaa tataaagaat ttttacctgt aacagatcaa
      301 acaccggcgc taactttaca tgaaggaaac acacctctta ttcacctgcc gaagctgtct
      361 gagcagctcg gaattgagct tcatgtcaaa acggaaggcg tcaatcctac gggatcattt
      421 aaagatcgcg gaatggttat ggctgtggca aaggcaaaag aagaaggcaa tgacacgatt
      481 atgtgcgcgt caacaggtaa cacttccgct gctgcggcag catatgcagc ccgtgctaac
      541 atgaaatgca ttgtcatcat cccgaacgga aaaattgcat ttggaaaact cgctcaagct
      601 gtcatgtacg gagccgagat tatcgcaatt gacggaaact ttgacgatgc gcttaaaatt
      661 gtccgttcca tctgtgagaa atcaccgatt gcccttgtca actcagtcaa cccttaccgc
      721 attgaaggcc aaaaaactgc tgccttcgaa atagtacata atggatttcc ttacgcgaaa
      781 tacgggcaga catggcctgc ccggttatta ttatttttga caccagacca actggtaatg
      841 gtagcgaccg gcgctcagct gtaattccgc cgatactgac gggctccagg agtcgtcgcc
      901 accaatcccc atatggaaac cgtcgatatt cagccatgtg ccttcttccg cgtgcagcag
      961 atggcgatgg ctggtttcca tcagttgctg ttgactgtag cggctgatgt tgaactggaa
     1021 gtcgccgcgc cactggtgtg ggccataatt caattcgcgc gtcccgcagc gcagaccgtt
     1081 ttcgctcggg aagacgtacg gggtatacat gtaccggttg tacactagtg cattagcggc
     1141 cggatcctcc tccacctttc gctgctgctt ctcctccacc tgaaaagaac gtaacgcttt
     1201 ctactttgtc tacatcaatt aatgttgtag cttggcctgc cggagtgacg gagctttcct
     1261 gtgttacaaa tacgatcccg tgaacctgat cgaaagcaac gaaaacaacc ggtccgatca
     1321 tgtcacctga atcagtcaca atgctgattg tagtgcctgg cgtaattctg gcaaaaatcc
     1381 atcttgccgg aagagaaggg tctaaaatag attctgccgt aaccgtttca ctttcatcta
     1441 ctttttgatc gatggcagca tcaagcaagg ctttaagatt actatccata ttttctcctc
     1501 cttctgttgc agttggaaat aaagggctta aaacggcttt aattaaatcc ttttttaact
     1561 gtgcattttc agagctcatt acacacacct cctattcgat agtgaactca cgttattcat
     1621 attcttccgg ctgttatttg gtatgagtcg aacgccgtac ctgatctatt tttcatctat
     1681 ttaaggaaaa tgctgatttt ttaatggagt ggacatagcc tataccataa atcgaagtcg
     1741 cacatattgt gaaacagaga taaggataag ggagagggat gatttggatt tagagaaaca
     1801 agtggatcga gaagaaaacc aagatgatct gcattttatt ttgcttttgc tgattctgct
     1861 gatacgtcaa tcgtcttctg aaggaaatcc atctgacctc agacgccagc ttgtttttgc
     1921 aaaagagctg ggcgtaccgg tttcaaaagt caatttgatc aacggagata cacttcaaaa
     1981 tgtcatcgta aaagaagttc tgactgattt ggttattttc caaaacccgt taaataacac
     2041 gagatctcat gtttcactct caagtgttgt aagctggggt gctttttaaa tgatgacaga
     2101 ggctctgctt gaattctttt caaaaaagta ttgacctagt taactaaaaa tgttactatt
     2161 aagtagtcgc caaggaggtg agacccatgc ttgaaaaaat catcaaacaa aagaaagaag
     2221 aagtgaaaac actggttctg ccggtagagc agcctttcga gaaacgttca tttaaggagg
     2281 cgctggcaag cccgaatcgg tttatcgggt tgattgccga agtgaagaaa gcatcgccgt
     2341 caaaagggct tattaaagag gattttgtac ctgtgcagat tgcaaaagac tatgaggctg
     2401 cgaaggcaga tgcgatttcc gttttaacag acaccccgtt ttttcaaggg gaaaacagct
     2461 atttatcaga cgtaaagcgt gctgtttcga ttcctgtact tagaaaagat tttattattg
     2521 attctcttca agtagaggaa tcaagaagaa tcggagcgga tgccatattg ttaatcggcg
     2581 aggtgcttga tcccttacac cttcatgaat tatatcttga agcaggtgaa aaggggatgg
     2641 acgtgttagt ggaggttcat gatgcatcaa cgctagaaca aatattgaaa gtgttcacac
     2701 ccgacattct cggcgtaaat aatcgaaacc taaaaacgtt tgaaacatct gtaaagcaga
     2761 cagaacaaat cgcatctctc gttccgaaag aatccttgct tgtcagcgaa agcggaatcg
     2821 gttctttaga acatttaaca tttgtcaatg aacatggggc gcgagctgta cttatcggtg
     2881 aatcattgat gagacaaact tctcagcgta aagcaatcca tgctttgttt agggagtaat
     2941 acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag
     3001 tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccgttac ttatgagcaa
     3061 gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg
     3121 cttttgtaaa tttggaaagt tacacgttac taaagggaat gtagataaat tattaggtat
     3181 actactgaca gcttccaagg agctaaagag gtccctagcg cctacgggga atttgtatcg
     3241 aagtgtgcga acagctgggg gaagcgcctg atgttttagc gatcccggtc ggaaatgctg
     3301 gaaacatcac tgcgtactgg aagggcttca aggaatatca tgagaaaaac ggcacaggcc
     3361 ttccgaaaat gcgcggcttt gaagctgagg gtgcggcggc aatcgtgcgc aatgaagtga
     3421 ttgaaaatcc ggaaacaata gcgacagcca ttcgtatcgg aaacccggca agctgggaca
     3481 aagctgtaaa ggcagccgag gaatccaatg ggaaaattga cgaagtcact gatgatgaaa
     3541 tccttcacgc atatcagctg atcgcccgtg tagaaggcgt gtttgcagaa ccaggttctt
     3601 gcgcgtctat cgcaggagtg ctgaaacagg tgaaatccgg agaaattccg aaaggcagca
     3661 aggtcgtagc tgtgttaaca ggaaacggac tgaaagatcc gaacacagcg gtcgacattt
     3721 cagaaatcaa gcctgtcaca ttgccgactg atgaagacag catccttgaa tatgtaaaag
     3781 gagcggcccg tgtatgaacg aagccgacat gctgttctct gtcactgttc ccggaagcac
     3841 agctaaccta ggccccggct ttgattcagt cggaatggcg ctcagcagat atttgaagct
     3901 gaccgtcttt gaaagcgaca aatggtcttt tgaggctgaa acagaaacag tcgccggaat
     3961 taattcgagc tcctttaact tcctcaccag tagttgtatc ggtaccataa gtagaagcag
     4021 caacccaagt agctttacca gcatccggtt caaccagcat agtaagaatc ttactggaca
     4081 tcggcagttc ttcgaacagt gcgccaacta ccagctcttt ctgcagttca ttcagggcac
     4141 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     4201 ttgatccatg gattacgcgt taacccgggc ccgcggatgc atatgatcag atcttaaggc
     4261 ctaggtctag aggatcgatc tgtataataa agaataatta ttaatctgta gacaaattgt
     4321 gaaaggatgt acttaaacgc taacggtcag ctttattgaa cagtaattta agtatatgtc
     4381 caatctaggg taagtaaatt gagtatcaat ataaacttta tatgaacata atcaacgagg
     4441 tgaaatcatg agcaatttga ttaacggaaa aataccaaat caagcgattc aaacattaaa
     4501 aatcgtaaaa gatttatttg gaagttcaat agttggagta tatctatttg gttcagcagt
     4561 aaatggtggt ttacgcatta acagcgatgt agatgttcta gtcgtcgtga atcatagttt
     4621 acctcaatta actcgaaaaa aactaacaga aagactaatg actatatcag gaaagattgg
     4681 aaatacggat tctgttagac cacttgaagt tacggttata aataggagtg aagttgtccc
     4741 ttggcaatat cctccaaaaa gagaatttat atacggtgag tggctcaggg gtgaatttga
     4801 gaatggacaa attcaggaac caagctatga tcctgatttg gctattgttt tagcacaagc
     4861 aagaaagaat agtatttctc tatttggtcc tgattcttca agtatacttg tctccgtacc
     4921 tttgacagat attcgaagag caattaagga ttctttgcca gaactaattg aggggataaa
     4981 aggtgatgag cgtaatgtaa ttttaaccct agctcgaatg tggcaaacag tgactactgg
     5041 tgaaattacc tcgaaagatg tcgctgcaga atgggctata cctcttttac ctaaagagca
     5101 tgtaacttta ctggatatag ctagaaaagg ctatcgggga gagtgtgatg ataagtggga
     5161 aggactatat tcaaaggtga aagcactcgt taagtatatg aaaaattcta tagaaacttc
     5221 tctcaattag gctaatttta ttgcaataac aggtgcttac ttttctggag ttctttagca
     5281 aattttttta ttagctgaac ttagtattag tggccatact cctccaatcc aaagctattt
     5341 agaaagatta ctatatcctc aaacaggcgg taaccggcct cttcatcggg aatgcgcgcg
     5401 accttcagca tcgccggcat gtccccctgg cggacgggaa gtatccagct cgaggtcggg
     5461 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5521 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5581 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5641 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5701 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5761 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5821 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5881 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5941 tgctgaagcc agttaccttc ggaaaaagag ttgatagctc ttgatccggc aaacaaacca
     6001 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6061 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6121 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6181 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6241 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6301 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6361 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6421 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6481 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6541 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6601 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6661 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6721 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6781 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6841 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6901 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6961 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7021 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7081 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7141 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7201 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     7261 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaatt
//
