LOCUS       V008546                 7228 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008546
VERSION     V008546
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 7228)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7228)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advance v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-NOV-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             247..879
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label="thrC"
                     /note="N terminal part of thrC"
                     /protein_id="AHG54639.1"
                     /translation="MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIEL
                     HVKTEGVNPTGSFKDRGMVMAVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVI
                     IPNGKIAFGKLAQAVMYGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIEGQK
                     TAAFEIVHNGFPYAKYGQTWPARLLLFLTPDQLVMVATGAQLELRRY"
     gene            247..879
                     /gene="thrC"
                     /label="thrC"
     misc_feature    complement(1124..1149)
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     gene            complement(1135..1612)
                     /gene="cgeA"
                     /label="cgeA"
     CDS             complement(1135..1548)
                     /codon_start=1
                     /gene="cgeA"
                     /product="CgeA"
                     /label="cgeA"
                     /note="spore coat protein"
                     /protein_id="AHG54636.1"
                     /translation="MSSENAQLKKDLIKAVLSPLFPTATEGGENMDSNLKALLDAAIDQ
                     KVDESETVTAESILDPSLPARWIFARITPGTTISIVTDSGDMIGPVVFVAFDQVHGIVF
                     VTQESSVTPAGQATTLIDVDKVESVTFFSGSGR"
     regulatory      complement(1557..1562)
                     /gene="cgeA"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(1584..1592)
                     /gene="cgeA"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(1609..1612)
                     /gene="cgeA"
                     /regulatory_class="minus_35_signal"
     CDS             2109..2861
                     /codon_start=1
                     /gene="trpC"
                     /product="TrpC"
                     /label="trpC"
                     /protein_id="AHG54640.1"
                     /translation="MLEKIIKQKKEEVKTLVLPVEQPFEKRSFKEAPASPNRFIGLIAE
                     VKKASPSKGLIKEDFVPVQIAKDYEAAKADAISVLTDTPFFQGENSYLSDVKRAVSIPV
                     LRKDFIIDSLQVEESRRIGADAILLIGEVLDPLHLHELYLEAGEKGMDVLVEVHDASTL
                     EQILKVFTPDILGVNNRNLKTFETSVKQTEQIASLVPKESLLVSESGIGSLEHLTFVNE
                     HGARAVLIGESLMRQTSQRKAIHALFRE"
     gene            2109..2861
                     /gene="trpC"
                     /label="trpC"
     CDS             3291..3719
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label="thrC"
                     /note="C-terminal part of thrC"
                     /protein_id="AHG54638.1"
                     /translation="MRGFEAEGAAAIVRNEVIENPETIATAIRIGNPASWDKAVKAAEE
                     SNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVKSGEIPKGSKVVAVLT
                     GNGLKDPNTAVDISEIKPVTLPTDEDSILEYVKGAARV"
     gene            3291..3719
                     /gene="thrC"
                     /label="thrC"
     CDS             3716..4126
                     /codon_start=1
                     /gene="thrB"
                     /product="ThrB"
                     /label="thrB"
                     /note="N-terminal part of thrB"
                     /protein_id="AHG54637.1"
                     /translation="MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWS
                     FEAETETVAGINSSSFNFLTSSCIGTISRSSNPSSFTSIRFNQHSKNLTGHRQFFEQCA
                     NYQLFLQFIQGTGEPACNPSCSIMRNDPHPVS"
     gene            3716..4126
                     /gene="thrB"
                     /label="thrB"
     CDS             4370..5149
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     rep_origin      complement(5399..5987)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6161..7018)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7019..7123)
                     /label="AmpR promoter"
ORIGIN
        1 aattcatgta aaagatgagg ttggttcatt ctcgaaaatt acatctgtgt tctcagagcg
       61 gggcgtgacg tttgaaaaaa tccttcagct gccaattaaa ggccatgatg agttagctga
      121 aatcgtaatt gtcacacatc atacatcaga agctgatttc agtgatatcc tgcaaaacct
      181 aaatgatttg gaagtcgttc aagaagtcaa aagcacatat cgtgtagaag ggaacggttg
      241 gagctaatgt ggaaaggact tatccatcaa tataaagaat ttttacctgt aacagatcaa
      301 acaccggcgc taactttaca tgaaggaaac acacctctta ttcacctgcc gaagctgtct
      361 gagcagctcg gaattgagct tcatgtcaaa acggaaggcg tcaatcctac gggatcattt
      421 aaagatcgcg gaatggttat ggctgtggca aaggcaaaag aagaaggcaa tgacacgatt
      481 atgtgcgcgt caacaggtaa cacttccgct gctgcggcag catatgcagc ccgtgctaac
      541 atgaaatgca ttgtcatcat cccgaacgga aaaattgcat ttggaaaact cgctcaagct
      601 gtcatgtacg gagccgagat tatcgcaatt gacggaaact ttgacgatgc gcttaaaatt
      661 gtccgttcca tctgtgagaa atcaccgatt gcccttgtca actcagtcaa cccttaccgc
      721 attgaaggcc aaaaaactgc tgccttcgaa atagtacata atggatttcc ttacgcgaaa
      781 tacgggcaga catggcctgc ccggttatta ttatttttga caccagacca actggtaatg
      841 gtagcgaccg gcgctcagct ggaactccgc cgatactgac gggctccagg agtcgtcgcc
      901 accaatcccc atatggaaac cgtcgatatt cagccatgtg ccttcttccg cgtgcagcag
      961 atggcgatgg ctggtttcca tcagttgctg ttgactgtag cggctgatgt tgaactggaa
     1021 gtcgccgcgc cactggtgtg ggccataatt caattcgcgc gtcccgcagc gcagaccgtt
     1081 ttcgctcggg aagacgtacg gggtatacat gtaccggttg tacactagtg catgctagcg
     1141 gccggatcct gaaaagaacg taacgctttc tactttgtct acatcaatta atgttgtagc
     1201 ttggcctgcc ggagtgacgg agctttcctg tgttacaaat acgatcccgt gaacctgatc
     1261 gaaagcaacg aaaacaaccg gtccgatcat gtcacctgaa tcagtcacaa tgctgattgt
     1321 agtgcctggc gtaattctgg caaaaatcca tcttgccgga agagaagggt ctaaaataga
     1381 ttctgccgta accgtttcac tttcatctac tttttgatcg atggcagcat caagcaaggc
     1441 tttaagatta ctatccatat tttctcctcc ttctgttgca gttggaaata aagggcttaa
     1501 aacggcttta attaaatcct tttttaactg tgcattttca gagctcatta cacacacctc
     1561 ctattcgata gtgaactcac gttattcata ttcttccggc tgttatttgg tatgagtcga
     1621 acgccgtacc tgatctattt ttcatctatt taaggaaaat gctgattttt taatggagtg
     1681 gacatagcct ataccataaa tcgaagtcgc acatattgtg aaacagagat aaggataagg
     1741 gagagggatg atttggattt agagaaacaa gtggatcgag aagaaaacca agatgatctg
     1801 cattttattt tgcttttgct gattctgctg atacgtcaat cgtcttctga aggaaatcca
     1861 tctgacctca gacgccagct tgtttttgca aaagagctgg gcgtaccggt ttcaaaagtc
     1921 aatttgatca acggagatac acttcaaaat gtcatcgtaa aagaagttct gactgatttg
     1981 gttattttcc aaaacccgtt aaataacacg agatctcatg tttcactctc aagaattctt
     2041 ttcaaaaaag tattgaccta gttaactaaa aatgttacta ttaagtagtc gccaaggagg
     2101 tgagacccat gcttgaaaaa atcatcaaac aaaagaaaga agaagtgaaa acactggttc
     2161 tgccggtaga gcagcctttc gagaaacgtt catttaagga ggcgccggca agcccgaatc
     2221 ggtttatcgg gttgattgcc gaagtgaaga aagcatcgcc gtcaaaaggg cttattaaag
     2281 aggattttgt acctgtgcag attgcaaaag actatgaggc tgcgaaggca gatgcgattt
     2341 ccgttttaac agacaccccg ttttttcaag gggaaaacag ctatttatca gacgtaaagc
     2401 gtgctgtttc gattcctgta cttagaaaag attttattat tgattctctt caagtagagg
     2461 aatcaagaag aatcggagcg gatgccatat tgttaatcgg cgaggtgctt gatcccttac
     2521 accttcatga attatatctt gaagcaggtg aaaaggggat ggacgtgtta gtggaggttc
     2581 atgatgcatc aacgctagaa caaatattga aagtgttcac acccgacatt ctcggcgtaa
     2641 ataatcgaaa cctaaaaacg tttgaaacat ctgtaaagca gacagaacaa atcgcatctc
     2701 tcgttccgaa agaatccttg cttgtcagcg aaagcggaat cggttcttta gaacatttaa
     2761 catttgtcaa tgaacatggg gcgcgagctg tacttatcgg tgaatcattg atgagacaaa
     2821 cttctcagcg taaagcaatc catgctttgt ttagggagta atacgtactt tgtttcaaaa
     2881 tgggtcaatc gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa
     2941 cacgccaaag taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat
     3001 agttatctat tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa
     3061 gttacacgtt actaaaggga atgtagataa attattaggt atactactga cagcttccaa
     3121 ggagctaaag aggtccctag cgcctacggg gaatttgtat cgaagtgtgc gaacagctgg
     3181 gggaagcgcc tgatgtttta gcgatcccgg tcggaaatgc tggaaacatc actgcgtact
     3241 ggaagggctt caaggaatat catgagaaaa acggcacagg ccttccgaaa atgcgcggct
     3301 ttgaagctga gggtgcggcg gcaatcgtgc gcaatgaagt gattgaaaat ccggaaacaa
     3361 tagcgacagc cattcgtatc ggaaacccgg caagctggga caaagctgta aaggcagccg
     3421 aggaatccaa tgggaaaatt gacgaagtca ctgatgatga aatccttcac gcatatcagc
     3481 tgatcgcccg tgtagaaggc gtgtttgcag aaccaggttc ttgcgcgtct atcgcaggag
     3541 tgctgaaaca ggtgaaatcc ggagaaattc cgaaaggcag caaggtcgta gctgtgttaa
     3601 caggaaacgg actgaaagat ccgaacacag cggtcgacat ttcagaaatc aagcctgtca
     3661 cattgccgac tgatgaagac agcatccttg aatatgtaaa aggagcggcc cgtgtatgaa
     3721 cgaagccgac atgctgttct ctgtcactgt tcccggaagc acagctaacc taggccccgg
     3781 ctttgattca gtcggaatgg cgctcagcag atatttgaag ctgaccgtct ttgaaagcga
     3841 caaatggtct tttgaggctg aaacagaaac agtcgccgga attaattcga gctcctttaa
     3901 cttcctcacc agtagttgta tcggtaccat aagtagaagc agcaacccaa gtagctttac
     3961 cagcatccgg ttcaaccagc atagtaagaa tcttactgga catcggcagt tcttcgaaca
     4021 gtgcgccaac taccagctct ttctgcagtt cattcagggc accggagaac ctgcgtgcaa
     4081 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcca tggattacgc
     4141 gttaacccgg gcccgcggat gcatatgatc agatcttaag gcctaggtct agaggatcga
     4201 tctgtataat aaagaataat tattaatctg tagacaaatt gtgaaaggat gtacttaaac
     4261 gctaacggtc agctttattg aacagtaatt taagtatatg tccaatctag ggtaagtaaa
     4321 ttgagtatca atataaactt tatatgaaca taatcaacga ggtgaaatca tgagcaattt
     4381 gattaacgga aaaataccaa atcaagcgat tcaaacatta aaaatcgtaa aagatttatt
     4441 tggaagttca atagttggag tatatctatt tggttcagca gtaaatggtg gtttacgcat
     4501 taacagcgat gtagatgttc tagtcgtcgt gaatcatagt ttacctcaat taactcgaaa
     4561 aaaactaaca gaaagactaa tgactatatc aggaaagatt ggaaatacgg attctgttag
     4621 accacttgaa gttacggtta taaataggag tgaagttgtc ccttggcaat atcctccaaa
     4681 aagagaattt atatacggtg agtggctcag gggtgaattt gagaatggac aaattcagga
     4741 accaagctat gatcctgatt tggctattgt tttagcacaa gcaagaaaga atagtatttc
     4801 tctatttggt cctgattctt caagtatact tgtctccgta cctttgacag atattcgaag
     4861 agcaattaag gattctttgc cagaactaat tgaggggata aaaggtgatg agcgtaatgt
     4921 aattttaacc ctagctcgaa tgtggcaaac agtgactact ggtgaaatta cctcgaaaga
     4981 tgtcgctgca gaatgggcta tacctctttt acctaaagag catgtaactt tactggatat
     5041 agctagaaaa ggctatcggg gagagtgtga tgataagtgg gaaggactat attcaaaggt
     5101 gaaagcactc gttaagtata tgaaaaattc tatagaaact tctctcaatt aggctaattt
     5161 tattgcaata acaggtgctt acttttctgg agttctttag caaatttttt tattagctga
     5221 acttagtatt agtggccata ctcctccaat ccaaagctat ttagaaagat tactatatcc
     5281 tcaaacaggc ggtaaccggc ctcttcatcg ggaatgcgcg cgaccttcag catcgccggc
     5341 atgtccccct ggcggacggg aagtatccag ctcgaggtcg ggccgcgttg ctggcgtttt
     5401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5581 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5761 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5821 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5881 tcggaaaaag agttgatagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5941 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6001 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6061 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6121 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6181 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6241 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6301 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6361 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6421 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     6481 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6541 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6601 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6661 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6721 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     6781 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6841 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6901 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6961 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7021 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7081 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7141 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     7201 tcacgaggcc ctttcgtctt caagaatt
//
