LOCUS       40924_10371        6860 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCeMM-NTAP(GS), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Burckstummer T, Bennett KL, Preradovic A, Schutze G, Hantschel O, 
            Superti-Furga G, Bauch A.
  TITLE     An efficient tandem affinity purification procedure for interaction 
            proteomics in mammalian cells
  JOURNAL   Nat. Methods 3 (12), 1013-1019 (2006)
  PUBMED    17060908
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Burckstummer T, Bennett KL, Preradovic A, Schutze G, Hantschel O, 
            Superti-Furga G, Bauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2007) Director's Laboratory, Research Center for 
            Molecular Medicine, Lazarettgasse 19, Vienna 1090, Austria
REFERENCE   3  (bases 1 to 6860)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6860)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2006"; volume: "3"; issue: "12"; pages: "1013-1019"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-MAR-2007) Director's Laboratory, Research Center for Molecular 
            Medicine, Lazarettgasse 19, Vienna 1090, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             380..400
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     CDS             407..520
                     /codon_start=1
                     /label=SBP
                     /note="streptavidin-binding peptide"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     misc_feature    607..1192
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1193..1909
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     LTR             2078..2669
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     CDS             2754..3611
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3785..4373
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4661..4682
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4697..4727
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4735..4751
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4759..4775
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4857..5160
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5165..5368
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     repeat_region   5369..5554
                     /label=5' LTR
                     /note="5' LTR"
     misc_feature    5607..5964
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             6029..6445
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     promoter        6792..6810
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 catgggcacc cccgcagtca ccacctacaa gctggtcatt aacggcaaga ctctgaaggg
       61 cgagaccacc accaaggccg tggacgcaga aaccgcggaa aaggcgttca agcagtacgc
      121 gaacgacaac ggggtggacg gagtctggac ctacgatgac gctacaaaaa cgttcaccgt
      181 gaccgaggtt aacacccccg ctgtgactac gtacaagctg gtgatcaatg ggaagaccct
      241 gaagggcgag accaccacga aagctgtaga cgccgagaca gccgagaagg ccttcaagca
      301 gtacgccaat gacaacggcg tggatggcgt gtggacctat gacgacgcca cgaagacatt
      361 cactgtgact gagatcgatg agaacctcta cttccagggc ggcagtatgg acgagaagac
      421 caccggctgg agaggcggcc acgtggtcga gggactggcc ggtgagctgg agcagctacg
      481 ggcccgcctt gagcaccacc cccaggggca gcgcgagccg ggccggccgg cgcgccatat
      541 ggctagcggc catcaaacaa gtttgtactc gagggccggc gcgccgcggc cgctacgtaa
      601 attccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
      661 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
      721 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
      781 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
      841 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
      901 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
      961 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     1021 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     1081 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac
     1141 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag
     1201 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1261 gaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1321 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1381 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     1441 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1501 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1561 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1621 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1681 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1741 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1801 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1861 gttcgtgacc gccgccggga tcactcacgg catggacgag ctgtacaagt aaagcgccag
     1921 cacagtggtc gagatccgga ttagtccaat ttgttaaaga caggatatca gtggtccagg
     1981 ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa
     2041 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     2101 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa
     2161 tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga
     2221 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat
     2281 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     2341 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag
     2401 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     2461 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     2521 ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
     2581 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     2641 attgactacc cgtcagcggg ggtctttcac acatgcagca tgtatcaaaa ttaatttggt
     2701 tttttttctt aagtatttac attaaatggc catagtattg aaaaaggaag agtatgagta
     2761 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2821 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2881 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2941 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3001 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3061 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3121 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3181 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3241 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3301 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3361 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3421 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3481 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3541 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3601 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3661 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3721 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3781 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3841 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3901 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3961 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4021 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4081 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4141 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4201 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4261 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4321 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4381 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4441 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4501 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4561 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4621 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     4681 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     4741 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cttgactagt
     4801 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4861 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4921 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4981 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     5041 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     5101 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     5161 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     5221 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     5281 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     5341 acggtgggag gtctatataa gcagagctca ataaaagagc ccacaacccc tcactcggcg
     5401 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc
     5461 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     5521 acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag
     5581 ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg tctgtccgat
     5641 tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc taactagctc
     5701 tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg
     5761 agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca aaaatcccga
     5821 tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc tggtaggaga
     5881 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag
     5941 ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     6001 tctgtatttg tctgaaaata tgggcccggg ccagactgtt accactccct taagtttgac
     6061 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     6121 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     6181 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     6241 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     6301 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     6361 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     6421 agccctcact ccttctctag gcgcccccat atggccatat gagatcttat atggggcacc
     6481 cccgcccctt gtaaacttcc ctgaccctga catgacaaga gttactaaca gcccctctct
     6541 ccaagctcac ttacaggctc tctacttagt ccagcacgaa gtctggagac ctctggcggc
     6601 agcctaccaa gaacaactgg accgaccggt ggtacctcac ccttaccgag tcggcgacac
     6661 agtgtgggtc cgccgacacc agactaagaa cctagaacct cgctggaaag gaccttacac
     6721 agtcctgctg accaccccca ccgccctcaa agtagacggc atcgcagctt ggatacacgc
     6781 cgcccacgtg ttaatacgac tcactatagg gaaggctgcc gaccccgggg gtggaccatc
     6841 ctctagactg ccatggaatt
//
