LOCUS       40924_10361        6884 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCeMM-CTAP(SG), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Burckstummer T, Bennett KL, Preradovic A, Schutze G, Hantschel O, 
            Superti-Furga G, Bauch A.
  TITLE     An efficient tandem affinity purification procedure for interaction 
            proteomics in mammalian cells
  JOURNAL   Nat. Methods 3 (12), 1013-1019 (2006)
  PUBMED    17060908
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Burckstummer T, Bennett KL, Preradovic A, Schutze G, Hantschel O, 
            Superti-Furga G, Bauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2007) Director's Laboratory, Research Center for 
            Molecular Medicine, Lazarettgasse 19, Vienna 1090, Austria
REFERENCE   3  (bases 1 to 6884)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6884)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2006"; volume: "3"; issue: "12"; pages: "1013-1019"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-MAR-2007) Director's Laboratory, Research Center for Molecular 
            Medicine, Lazarettgasse 19, Vienna 1090, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             23..52
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             53..166
                     /codon_start=1
                     /label=SBP
                     /note="streptavidin-binding peptide"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             173..193
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     CDS             200..220
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     misc_feature    635..1220
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1221..1937
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     LTR             2106..2697
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     CDS             2782..3639
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3813..4401
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4689..4710
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4725..4755
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4763..4779
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4787..4803
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4885..5188
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5193..5396
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     repeat_region   5397..5582
                     /label=5' LTR
                     /note="5' LTR"
     misc_feature    5635..5992
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             6057..6473
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     promoter        6820..6838
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aattcggttt aaacggccgg ccgagcagaa gcttatctcc gaggaggatc tcatggacga
       61 gaagaccacc gggtggcggg gcggccacgt tgtggagggt ctcgctggcg agctggagca
      121 gctcagggcc cgcttggagc accatcccca ggggcaacgc gagcctatcg atgaaaatct
      181 gtacttccag gggatcgatg agaacctgta ctttcagggg ggtagtaccc cggccgtgac
      241 gacctacaag ctggtgatca acggcaagac cctgaagggc gagaccacaa ccaaggccgt
      301 ggacgctgaa accgcagaga aggccttcaa gcagtacgcc aacgacaatg gcgtggacgg
      361 agtgtggacc tacgacgatg ccaccaagac cttcaccgtg accgaggtta acacccccgc
      421 agtcaccaca tacaaactgg tgatcaacgg aaaaacgctg aagggcgaaa ctacgaccaa
      481 ggctgtggac gcggagacgg ccgaaaaggc gttcaaacag tatgccaacg ataacggtgt
      541 agacggcgtc tggacatatg acgacgcgac aaagactttc actgtcactg aatagctcga
      601 gggccggcgc gccgcggccg ctacgtaaat tccgcccctc tccctccccc ccccctaacg
      661 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
      721 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
      781 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
      841 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
      901 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
      961 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     1021 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     1081 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     1141 ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     1201 atgataatat ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1261 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg
     1321 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1381 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     1441 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1501 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1561 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1621 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     1681 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     1741 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1801 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     1861 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actcacggca
     1921 tggacgagct gtacaagtaa agcgccagca cagtggtcga gatccggatt agtccaattt
     1981 gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata tcaccagctg
     2041 aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct ccagaaaaag
     2101 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     2161 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
     2221 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     2281 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     2341 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
     2401 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     2461 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     2521 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
     2581 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     2641 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacac
     2701 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
     2761 tagtattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2821 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2881 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2941 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3001 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     3061 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3121 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3181 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3241 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3301 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3361 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3421 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3481 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     3541 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3601 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3661 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3721 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3781 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3841 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3901 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     3961 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4021 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4081 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4141 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4201 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4261 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4321 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4381 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4441 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4501 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4561 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     4621 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4681 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     4741 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     4801 gaccatgatt acgccaagct tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     4861 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     4921 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     4981 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     5041 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     5101 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     5161 ctacgtatta gtcatcgcta ttaccatgca tggtgatgcg gttttggcag tacatcaatg
     5221 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     5281 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     5341 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcaat
     5401 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
     5461 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     5521 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
     5581 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
     5641 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
     5701 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     5761 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
     5821 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
     5881 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     5941 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
     6001 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
     6061 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     6121 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     6181 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     6241 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     6301 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     6361 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     6421 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     6481 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     6541 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     6601 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     6661 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     6721 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     6781 tagacggcat cgcagcttgg atacacgccg cccacgtgtt aatacgactc actataggga
     6841 aggctgccga ccccgggggt ggaccatcct ctagactgcc atgg
//
