LOCUS       62056_4910       11634 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..951)
                     /codon_start=1
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      complement(999..1221)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     promoter        1867..1969
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2425..3447
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(3683..4558)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4585..4869
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             4928..5341
                     /codon_start=1
                     /label=Gam
                     /note="inhibitor of the host RecBCD nuclease in the lambda 
                     Red system"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             5350..6132
                     /codon_start=1
                     /label=Beta
                     /note="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             6132..6809
                     /codon_start=1
                     /label=Exo
                     /note="5' to 3' double-stranded DNA exonuclease in the 
                     lambda
                     Red system"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     CDS             7394..11497
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcggttgcgg ccgcaatcgg gcaaatcgct gaatattcct tttgtctccg
     1381 accatcaggc acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct
     1441 ctggcagtga atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa
     1501 ctacccataa tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg
     1561 ctatgtggtg ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc
     1621 tgaccacttc ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc
     1681 agcggtatca tcaacaggct tacccgtctt actgtcgggg atcgacgctc tcccttatgc
     1741 gactcctgca cctttcgtct tcgaataaat acctgtgacg gaagatcact tcgcagaata
     1801 aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg
     1861 agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
     1921 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     1981 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     2041 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctgggtc atccaccgga
     2101 tcaattcccc tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc
     2161 cttggagccc ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac
     2221 ccgcagttgc aaaccctcac tgatccggct cacggtaact gatgccgtat ttgcagtacc
     2281 agcgtacggc ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt
     2341 gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc
     2401 cgttgatcgt gctatgatcg actgatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     2461 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     2521 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     2581 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     2641 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     2701 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     2761 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     2821 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     2881 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     2941 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     3001 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     3061 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     3121 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     3181 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     3241 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     3301 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     3361 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     3421 cccagcactc gtccgagggc aaaggaatag tgagctcatg agcggagaac gagatgacgt
     3481 tggaggggca aggtcgcgct gattgctggg gcaacacgtg aaaggcgaga tcaccaaggt
     3541 agtcggcaaa taatgtctaa cggtacccgg ggatcctcta gagtcgacct gcataatgtg
     3601 cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa
     3661 ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac
     3721 aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat
     3781 agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc
     3841 tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc
     3901 ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga
     3961 cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc
     4021 gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta
     4081 acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg
     4141 cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa
     4201 agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg
     4261 gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat
     4321 gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct
     4381 gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca
     4441 gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca
     4501 ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac
     4561 ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc
     4621 gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa
     4681 gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata
     4741 atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag
     4801 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg
     4861 tttctccata cccgtttttt tgggctagcg aaaagatgtt tcgtgaagcc gtcgacgctt
     4921 ataaaaaatg gatattaata ctgaaactga gatcaagcaa aagcattcac taaccccctt
     4981 tcctgttttc ctaatcagcc cggcatttcg cgggcgatat tttcacagct atttcaggag
     5041 ttcagccatg aacgcttatt acattcagga tcgtcttgag gctcagagct gggcgcgtca
     5101 ctaccagcag ctcgcccgtg aagagaaaga ggcagaactg gcagacgaca tggaaaaagg
     5161 cctgccccag cacctgtttg aatcgctatg catcgatcat ttgcaacgcc acggggccag
     5221 caaaaaatcc attacccgtg cgtttgatga cgatgttgag tttcaggagc gcatggcaga
     5281 acacatccgg tacatggttg aaaccattgc tcaccaccag gttgatattg attcagaggt
     5341 ataaaacgaa tgagtactgc actcgcaacg ctggctggga agctggctga acgtgtcggc
     5401 atggattctg tcgacccaca ggaactgatc accactcttc gccagacggc atttaaaggt
     5461 gatgccagcg atgcgcagtt catcgcatta ctgatcgttg ccaaccagta cggccttaat
     5521 ccgtggacga aagaaattta cgcctttcct gataagcaga atggcatcgt tccggtggtg
     5581 ggcgttgatg gctggtcccg catcatcaat gaaaaccagc agtttgatgg catggacttt
     5641 gagcaggaca atgaatcctg tacatgccgg atttaccgca aggaccgtaa tcatccgatc
     5701 tgcgttaccg aatggatgga tgaatgccgc cgcgaaccat tcaaaactcg cgaaggcaga
     5761 gaaatcacgg ggccgtggca gtcgcatccc aaacggatgt tacgtcataa agccatgatt
     5821 cagtgtgccc gtctggcctt cggatttgct ggtatctatg acaaggatga agccgagcgc
     5881 attgtcgaaa atactgcata cactgcagaa cgtcagccgg aacgcgacat cactccggtt
     5941 aacgatgaaa ccatgcagga gattaacact ctgctgatcg ccctggataa aacatgggat
     6001 gacgacttat tgccgctctg ttcccagata tttcgccgcg acattcgtgc atcgtcagaa
     6061 ctgacacagg ccgaagcagt aaaagctctt ggattcctga aacagaaagc cgcagagcag
     6121 aaggtggcag catgacaccg gacattatcc tgcagcgtac cgggatcgat gtgagagctg
     6181 tcgaacaggg ggatgatgcg tggcacaaat tacggctcgg cgtcatcacc gcttcagaag
     6241 ttcacaacgt gatagcaaaa ccccgctccg gaaagaagtg gcctgacatg aaaatgtcct
     6301 acttccacac cctgcttgct gaggtttgca ccggtgtggc tccggaagtt aacgctaaag
     6361 cactggcctg gggaaaacag tacgagaacg acgccagaac cctgtttgaa ttcacttccg
     6421 gcgtgaatgt tactgaatcc ccgatcatct atcgcgacga aagtatgcgt accgcctgct
     6481 ctcccgatgg tttatgcagt gacggcaacg gccttgaact gaaatgcccg tttacctccc
     6541 gggatttcat gaagttccgg ctcggtggtt tcgaggccat aaagtcagct tacatggccc
     6601 aggtgcagta cagcatgtgg gtgacgcgaa aaaatgcctg gtactttgcc aactatgacc
     6661 cgcgtatgaa gcgtgaaggc ctgcattatg tcgtgattga gcgggatgaa aagtacatgg
     6721 cgagttttga cgagatcgtg ccggagttca tcgaaaaaat ggacgaggca ctggctgaaa
     6781 ttggttttgt atttggggag caatggcgat gacgcatcct cacgataata agcttcctgc
     6841 tgaacatcaa aggcaagaaa acatctgttg tcaaagacag catccttgaa caaggacaat
     6901 taacagttaa caaataaaaa cgcaaaagaa aatgccgata ttgactaccg gaagcagtgt
     6961 gaccgtgtgc ttctcaaatg cctgattcag gctgtctatg tgtgactgtt gagctgtaac
     7021 aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg tttcacctgt
     7081 tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat ggtgaacagc
     7141 tttaaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta caccgttttc
     7201 atctgtgcat atagctatac tgatttcgtc agactcacag tcaaacatgc cggtcagttg
     7261 gcctggtgat ggcgggatcg ttgtatattt cttgacacct tttcggcacc gccctaaaat
     7321 tctgcgtcct cataatatat gaggcgattt attacgtgtt tacgaagcaa aagctaaaac
     7381 caggagctat ttaatggata agaaatactc aataggctta gatatcggca caaatagcgt
     7441 cggatgggcg gtgatcactg atgaatataa ggttccgtct aaaaagttca aggttctggg
     7501 aaatacagac cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg
     7561 agagacagcg gaagcgactc gtctcaaacg gacagctcgt agaaggtata cacgtcggaa
     7621 gaatcgtatt tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag
     7681 tttctttcat cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca
     7741 tcctattttt ggaaatatag tagatgaagt tgcttatcat gagaaatatc caactatcta
     7801 tcatctgcga aaaaaattgg tagattctac tgataaagcg gatttgcgct taatctattt
     7861 ggccttagcg catatgatta agtttcgtgg tcattttttg attgagggag atttaaatcc
     7921 tgataatagt gatgtggaca aactatttat ccagttggta caaacctaca atcaattatt
     7981 tgaagaaaac cctattaacg caagtggagt agatgctaaa gcgattcttt ctgcacgatt
     8041 gagtaaatca agacgattag aaaatctcat tgctcagctc cccggtgaga agaaaaatgg
     8101 cttatttggg aatctcattg ctttgtcatt gggtttgacc cctaatttta aatcaaattt
     8161 tgatttggca gaagatgcta aattacagct ttcaaaagat acttacgatg atgatttaga
     8221 taatttattg gcgcaaattg gagatcaata tgctgatttg tttttggcag ctaagaattt
     8281 atcagatgct attttacttt cagatatcct aagagtaaat actgaaataa ctaaggctcc
     8341 cctatcagct tcaatgatta aacgctacga tgaacatcat caagacttga ctcttttaaa
     8401 agctttagtt cgacaacaac ttccagaaaa gtataaagaa atcttttttg atcaatcaaa
     8461 aaacggatat gcaggttata ttgatggggg agctagccaa gaagaatttt ataaatttat
     8521 caaaccaatt ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac taaatcgtga
     8581 agatttgctg cgcaagcaac ggacctttga caacggctct attccccatc aaattcactt
     8641 gggtgagctg catgctattt tgagaagaca agaagacttt tatccatttt taaaagacaa
     8701 tcgtgagaag attgaaaaaa tcttgacttt tcgaattcct tattatgttg gtccattggc
     8761 gcgtggcaat agtcgttttg catggatgac tcggaagtct gaagaaacaa ttaccccatg
     8821 gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg aacgcatgac
     8881 aaactttgat aaaaatcttc caaatgaaaa agtactacca aaacatagtt tgctttatga
     8941 gtattttacg gtttataacg aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa
     9001 accagcattt ctttcaggtg aacagaagaa agccattgtt gatttactct tcaaaacaaa
     9061 tcgaaaagta accgttaagc aattaaaaga agattatttc aaaaaaatag aatgttttga
     9121 tagtgttgaa atttcaggag ttgaagatag atttaatgct tcattaggta cctaccatga
     9181 tttgctaaaa attattaaag ataaagattt tttggataat gaagaaaatg aagatatctt
     9241 agaggatatt gttttaacat tgaccttatt tgaagatagg gagatgattg aggaaagact
     9301 taaaacatat gctcacctct ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta
     9361 tactggttgg ggacgtttgt ctcgaaaatt gattaatggt attagggata agcaatctgg
     9421 caaaacaata ttagattttt tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct
     9481 gatccatgat gatagtttga catttaaaga agacattcaa aaagcacaag tgtctggaca
     9541 aggcgatagt ttacatgaac atattgcaaa tttagctggt agccctgcta ttaaaaaagg
     9601 tattttacag actgtaaaag ttgttgatga attggtcaaa gtaatggggc ggcataagcc
     9661 agaaaatatc gttattgaaa tggcacgtga aaatcagaca actcaaaagg gccagaaaaa
     9721 ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa gtcagattct
     9781 taaagagcat cctgttgaaa atactcaatt gcaaaatgaa aagctctatc tctattatct
     9841 ccaaaatgga agagacatgt atgtggacca agaattagat attaatcgtt taagtgatta
     9901 tgatgtcgat cacattgttc cacaaagttt ccttaaagac gattcaatag acaataaggt
     9961 cttaacgcgt tctgataaaa atcgtggtaa atcggataac gttccaagtg aagaagtagt
    10021 caaaaagatg aaaaactatt ggagacaact tctaaacgcc aagttaatca ctcaacgtaa
    10081 gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata aagctggttt
    10141 tatcaaacgc caattggttg aaactcgcca aatcactaag catgtggcac aaattttgga
    10201 tagtcgcatg aatactaaat acgatgaaaa tgataaactt attcgagagg ttaaagtgat
    10261 taccttaaaa tctaaattag tttctgactt ccgaaaagat ttccaattct ataaagtacg
    10321 tgagattaac aattaccatc atgcccatga tgcgtatcta aatgccgtcg ttggaactgc
    10381 tttgattaag aaatatccaa aacttgaatc ggagtttgtc tatggtgatt ataaagttta
    10441 tgatgttcgt aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata
    10501 tttcttttac tctaatatca tgaacttctt caaaacagaa attacacttg caaatggaga
    10561 gattcgcaaa cgccctctaa tcgaaactaa tggggaaact ggagaaattg tctgggataa
    10621 agggcgagat tttgccacag tgcgcaaagt attgtccatg ccccaagtca atattgtcaa
    10681 gaaaacagaa gtacagacag gcggattctc caaggagtca attttaccaa aaagaaattc
    10741 ggacaagctt attgctcgta aaaaagactg ggatccaaaa aaatatggtg gttttgatag
    10801 tccaacggta gcttattcag tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa
    10861 gttaaaatcc gttaaagagt tactagggat cacaattatg gaaagaagtt cctttgaaaa
    10921 aaatccgatt gactttttag aagctaaagg atataaggaa gttaaaaaag acttaatcat
    10981 taaactacct aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag
    11041 tgccggagaa ttacaaaaag gaaatgagct ggctctgcca agcaaatatg tgaatttttt
    11101 atatttagct agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca
    11161 attgtttgtg gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt
    11221 ttctaagcgt gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa
    11281 acatagagac aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac
    11341 gaatcttgga gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata
    11401 tacgtctaca aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta
    11461 tgaaacacgc attgatttga gtcagctagg aggtgactga tggctggttg gcgtactgtt
    11521 gtggtaaata cccatatgga cagttttccc tttgatatgt aacggtgaac agttgttcta
    11581 cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag aacc
//
