LOCUS       40924_8941       10131 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCamE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Ma Z, Wu L, Wang X, Li X, Li Y, Wang Z.
  TITLE     Constructing a novel plant expression vector (pCamE) with CaMV 35S 
            promoter and fifteen restriction endonuclease sites in the 
            expression cassette
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10131)
  AUTHORS   Ma Z, Wu L, Wang X, Li X, Li Y, Wang Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2012) Crop Genetics and Breeding Department, 
            Agricultural University of Hebei, South of Jianshe Street 215, 
            Baoding, Hebei 071000, P.R. China
REFERENCE   3  (bases 1 to 10131)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10131)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-SEP-2012) Crop Genetics and Breeding Department, Agricultural 
            University of Hebei, South of Jianshe Street 215, Baoding, Hebei 
            071000, P.R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: DNASTAR v. 7.10
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..25)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(33..49)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(57..87)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(102..123)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        314..992
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             1058..2080
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     polyA_signal    2124..2298
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(2376..2400)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2825..3616
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3706..4294
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4480..4620)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4964..5158)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5227..6291)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6728..7354)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    complement(8656..8680)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8883..8899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9429..9774
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    9798..9830
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     regulatory      9851..10115
                     /label=NOS
                     /note="NOS"
                     /regulatory_class="terminator"
     terminator      9861..10113
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
       61 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      121 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      181 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag cagcttgcca
      241 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
      301 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
      361 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca
      421 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc
      481 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
      541 cttcaaagca agtggattga tgtgataaca tggtggagca cgacactctc gtctactcca
      601 agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
      661 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga
      721 cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg
      781 ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
      841 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
      901 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
      961 gaagttcatt tcatttggag aggacacgct gaaatcacca gtctctctct acaaatctat
     1021 ctctctcgag cttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc
     1081 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     1141 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     1201 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     1261 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
     1321 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     1381 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
     1441 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     1501 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     1561 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     1621 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     1681 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     1741 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     1801 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
     1861 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     1921 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     1981 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     2041 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg
     2101 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
     2161 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
     2221 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
     2281 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
     2341 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     2401 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     2461 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     2521 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     2581 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
     2641 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     2701 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     2761 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
     2821 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
     2881 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
     2941 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
     3001 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
     3061 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
     3121 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
     3181 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
     3241 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
     3301 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
     3361 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     3421 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     3481 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     3541 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     3601 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     3661 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3721 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3781 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3841 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3901 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3961 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4021 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4081 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4141 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4201 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4261 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4321 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4381 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4441 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     4501 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     4561 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     4621 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4681 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4741 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     4801 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     4861 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     4921 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     4981 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     5041 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     5101 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     5161 aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     5221 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     5281 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     5341 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     5401 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     5461 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     5521 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     5581 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     5641 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     5701 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     5761 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     5821 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     5881 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     5941 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     6001 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     6061 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     6121 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     6181 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     6241 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg
     6301 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
     6361 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     6421 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     6481 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     6541 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     6601 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     6661 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     6721 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     6781 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     6841 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     6901 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     6961 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     7021 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     7081 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     7141 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     7201 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     7261 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     7321 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     7381 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     7441 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     7501 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     7561 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     7621 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
     7681 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
     7741 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
     7801 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
     7861 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
     7921 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
     7981 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
     8041 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
     8101 aggtcgcggg tgctgcgggt ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
     8161 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
     8221 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
     8281 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
     8341 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
     8401 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
     8461 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
     8521 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
     8581 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     8641 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     8701 aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct
     8761 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     8821 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     8881 ttgtaaaacg acggccagtg ccaagcttaa ttaagagctc gcatgccctt tcaggtcccc
     8941 agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga gaggcttacg
     9001 cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc aaataccttc
     9061 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
     9121 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
     9181 acaaaccaag gcaagtaata gagattggaa gtctctaaaa aggtagttcc cactgaatca
     9241 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     9301 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     9361 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     9421 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     9481 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     9541 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     9601 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     9661 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     9721 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacgggga
     9781 ctctagcgct accggtcgtc gactctagag gatccccggg taccgggccc tgatctcgag
     9841 gagctagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     9901 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     9961 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
    10021 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
    10081 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aattcgaatt c
//
