LOCUS       40924_9021       12184 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Plant binary expression vector pCaS1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Toosi S, Shokouhifar F, Malekzadeh Shafaroudi S, Bagheri A.
  TITLE     Functional Analysis of Fol-SIX1 in Resistant and Susceptible Melon 
            Lines
  JOURNAL   Zist Fanavarii Giyahani Zirai 7 (4), 35-47 (2015)
REFERENCE   2  (bases 1 to 12184)
  AUTHORS   Toosi S, Shokouhifar F, Malekzadeh Shafaroudi S, Bagheri A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2015) Reserarch Center for Plant Sciences, 
            Ferdowsi University of Mashhad, Mashhad, Khorasan Razavi 91775-1653,
            Iran
REFERENCE   3  (bases 1 to 12184)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12184)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zist 
            Fanavarii Giyahani Zirai"; date: "2015"; volume: "7"; issue: "4"; 
            pages: "35-47"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2015) Reserarch Center for Plant Sciences, Ferdowsi 
            University of Mashhad, Mashhad, Khorasan Razavi 91775-1653, Iran"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: seqman v. 7.0.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(103..277)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(287..835)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(880..1557)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    1748..1769
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1784..1814
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1822..1838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1846..1862
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1872..1928
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1932..1948)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2325..2670
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2687..3541
                     /codon_start=1
                     /gene="Fol-SIX1"
                     /product="secreted in xylem 1 protein"
                     /label=Fol-SIX1
                     /protein_id="AJW67224.1"
                     /translation="MAPYSMVLLGALSILGFGAYAQEAAVREPQIFFNLTYTEYLDKVA
                     ASHGSPPDKSDLPWNDTMGSFPGNETDDGVQTETGSSLSRRGHIVNLRKREPFGEESRN
                     DRVTQDMLQALHDLCVERFGTGYRAVSGLCYTDRRATRKIECNKPSVRERDRSVTRACP
                     EGQECTTFNAYNFRNRHHQVTFPVCGPRIEVKDRHDIGIHTEWQGTWYPESPKSPGTYD
                     YFAQMAGTLNGYFGYDGVYSDGYKTSSHGYGHSWSCINCPRGKVTITNTYRATWAFGYT
                     SPH"
     gene            2687..3541
                     /gene="Fol-SIX1"
                     /label=Fol-SIX1
     intron          3567..3756
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             3751..5574
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LQNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQ
                     ESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWV
                     NNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQ
                     SYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELR
                     DADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLRVGIRSVAV
                     KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAE
                     EMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKE
                     LIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHT
                     DTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAG
                     LHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIF
                     TRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             5581..5598
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      5633..5885
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5907..5931
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7231..7857
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8294..9358
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      9427..9621
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9965..10105
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10291..10879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10969..11760)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gagtcaaatc tcggtgacgg
      301 gcaggaccgg acggggcggt accggcaggc tgaagtccag ctgccagaaa cccacgtcat
      361 gccagttccc gtgcttgaag ccggccgccc gcagcatgcc gcggggggca tatccgagcg
      421 cctcgtgcat gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc acgctcttga
      481 agccctgtgc ctccagggac ttcagcaggt gggtgtagag cgtggagccc agtcccgtcc
      541 gctggtggcg gggggagacg tacacggtcg actcggccgt ccagtcgtag gcgttgcgtg
      601 ccttccaggg gcccgcgtag gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc
      661 agggatagcg ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct
      721 cggtacggaa gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg cagaccgccg
      781 gcatgtccgc ctcggtggca cggcggatgt cggccgggcg tcgttctggg ctcatggtag
      841 actcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg
      901 aaatgaactt ccttatatag aggaaggtct tgcgaaggat agtgggattg tgcgtcatcc
      961 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
     1021 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
     1081 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
     1141 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
     1201 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
     1261 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttatcaca tcaatccact
     1321 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
     1381 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
     1441 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
     1501 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
     1561 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
     1621 tccaccatgt tggcaagctg ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc
     1681 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     1741 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     1801 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     1861 accatgatta cgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat
     1921 gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     1981 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2041 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta gagcagcttg
     2101 agcttggatc agattgtcgt ttcccgcctt cagtttagct tcatggagtc aaagattcaa
     2161 atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta
     2221 cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac
     2281 tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa
     2341 agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg
     2401 aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc
     2461 atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     2521 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     2581 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     2641 taaggaagtt catttcattt ggagagaaca cgggggactc ttgaccatgg cgccctatag
     2701 catggtactc cttggcgccc tctcaatcct tgggtttggg gcttatgctc aagaggctgc
     2761 ggttcgggag ccccagatat ttttcaactt gacctacacg gaatatctgg ataaggtggc
     2821 agcatcccac gggagtcccc cagacaagag tgatttgccc tggaatgaca ctatgggcag
     2881 ctttcctgga aacgagacgg acgatggtgt ccagaccgag acgggcagca gcctatcgag
     2941 gcgtggtcac attgtcaacc ttagaaagcg tgagcctttc ggggaggagt ctcgcaacga
     3001 tcgtgtcaca caagacatgc tccaggcgct tcatgatctg tgcgttgaaa gatttggcac
     3061 aggctatcga gcggttagtg ggttatgtta cactgatcgt cgggcgacta ggaaaatcga
     3121 atgtaataaa ccgtctgtca gggagagaga cagatcagtg accagagctt gcccagaggg
     3181 ccaggagtgc accactttca atgcgtacaa ctttcgtaac cgtcaccatc aggttacttt
     3241 tcctgtctgt gggccccgaa ttgaggtgaa ggacagacat gatataggga tacacacgga
     3301 gtggcaggga acctggtacc ccgaatcgcc taaatcgcct gggacctacg attatttcgc
     3361 ccagatggcg ggcactctca atgggtactt cgggtatgac ggtgtttatt cggatggata
     3421 taagacgagc tctcacggat atggacattc atggtcatgc attaattgcc cgagaggcaa
     3481 ggtgactatc acaaacacat atcgcgcaac ttgggcgttt ggatatacca gcccacacta
     3541 agttgcgatc catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg
     3601 ttaggaccct tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt
     3661 ttttaattga ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt
     3721 tatttcgtgt gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta
     3781 gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc
     3841 gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt
     3901 gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc
     3961 aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg
     4021 ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg
     4081 gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg
     4141 aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg
     4201 ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt
     4261 aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac
     4321 gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag
     4381 gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca
     4441 actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt
     4501 gaaggttatc tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac
     4561 ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac
     4621 aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga
     4681 ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc
     4741 taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc
     4801 atcgtggtga ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc
     4861 gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag
     4921 caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg
     4981 gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc
     5041 gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat
     5101 gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg
     5161 aaccgttatt acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg
     5221 gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac
     5281 ggcgtggata cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat
     5341 cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt
     5401 gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt
     5461 aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa
     5521 aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc
     5581 caccaccacc accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc
     5641 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     5701 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     5761 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     5821 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     5881 agatcgggaa ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa
     5941 aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     6001 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
     6061 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg
     6121 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
     6181 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
     6241 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
     6301 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
     6361 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
     6421 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
     6481 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
     6541 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
     6601 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
     6661 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
     6721 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
     6781 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
     6841 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
     6901 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
     6961 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
     7021 gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
     7081 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
     7141 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
     7201 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
     7261 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
     7321 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
     7381 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
     7441 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
     7501 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
     7561 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
     7621 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
     7681 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
     7741 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
     7801 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
     7861 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
     7921 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
     7981 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
     8041 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
     8101 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
     8161 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
     8221 ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag
     8281 gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
     8341 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
     8401 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
     8461 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
     8521 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
     8581 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
     8641 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
     8701 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
     8761 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
     8821 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
     8881 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
     8941 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
     9001 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
     9061 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
     9121 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
     9181 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
     9241 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
     9301 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
     9361 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct
     9421 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
     9481 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
     9541 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
     9601 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc
     9661 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
     9721 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
     9781 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg
     9841 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     9901 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     9961 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
    10021 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10081 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
    10141 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    10201 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10261 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10321 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10381 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10441 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10501 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10561 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10621 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10681 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    10741 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10801 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10861 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10921 gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc
    10981 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
    11041 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
    11101 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
    11161 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
    11221 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
    11281 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
    11341 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
    11401 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
    11461 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
    11521 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
    11581 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
    11641 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
    11701 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
    11761 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    11821 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    11881 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    11941 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
    12001 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
    12061 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
    12121 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
    12181 ctgg
//
