LOCUS       V008724                13376 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008724
VERSION     V008724
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Wang Y, Cong QQ, Lan YF, Geng C, Li XD, Liang YC, Yang ZY, Zhu XP,
            Li XD.
  TITLE     Development of new potato virus X-based vectors for gene
            over-expression and gene silencing assay
  JOURNAL   Virus Res. 191, 62-69 (2014)
   PUBMED   25076104
REFERENCE   2  (bases 1 to 13376)
  AUTHORS   Wang Y, Cong QQ, Li XD.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2014) Department of Plant Pathology, Shandong
            Agricultural University, No. 61 Daizong street, Tai'an, Shandong
            Province 271018, PR China
REFERENCE   3  (bases 1 to 13376)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13376)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res.
            191, 62-69 (2014)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-APR-2014) Department of Plant Pathology, Shandong Agricultural
            University, No. 61 Daizong street, Tai'an, Shandong Province 271018,
            PR China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           1..84
                     /label="derived from PVX isolate 1985"
                     /note="derived from PVX isolate 1985"
     CDS             85..4452
                     /note="RNA replication protein from Potato virus X.
                     Accession#: P09395"
                     /label="RNA replication protein"
     CDS             4486..5163
                     /note="Movement and silencing protein TGBp1 from Potato
                     virus X (strain X3). Accession#: P17780"
                     /label="Movement and silencing protein TGBp1"
     CDS             5147..5494
                     /codon_start=1
                     /gene="TGB2"
                     /product="triple gene block protein 2"
                     /label="TGB2"
                     /protein_id="AII77193.1"
                     /translation="MSAQGHRLTAPVNSEKVYIVLGLSFALVSITFLLSRSNLPHVGDN
                     IHSLPHGGAYRDGTKAILYNSPNLGSRVSLHNGKNAAFAAVLLLTLLIYGSKYISQRNH
                     TCACGNNHSSH"
     gene            5147..5494
                     /gene="TGB2"
                     /label="TGB2"
                     /note="derived from PVX isolate 1985"
     CDS             5427..5639
                     /codon_start=1
                     /gene="TGB3"
                     /product="triple gene block protein 3"
                     /label="TGB3"
                     /protein_id="AII77194.1"
                     /translation="MEVNTYLNAIILVLVVTIIAVISTSLVRTEPCVIKITGESITVLA
                     CKLDAETIRAIADLKPLSVERLSFH"
     gene            5427..5639
                     /gene="TGB3"
                     /label="TGB3"
                     /note="derived from PVX isolate 1985"
     RBS             5496..5504
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    5668..5691
                     /label="cloning site"
                     /note="cloning site"
     misc_feature    5692..5902
                     /note="derived from tobacco mosaic virus coat protein
                     subgenomic promoter"
     misc_feature    5903..5932
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      5933..6014
                     /label="derived from PVX isolate 1985"
                     /note="derived from PVX isolate 1985"
                     /regulatory_class="promoter"
     misc_feature    6009..6032
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     3'UTR           6033..6133
                     /label="derived from PVX isolate 1985"
                     /note="derived from PVX isolate 1985"
     CDS             6190..6207
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      6242..6494
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6516..6540
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7840..8466
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8903..9967
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      10036..10230
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10574..10714
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10900..11488)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(11578..12369)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    12794..12818
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        13033..13376
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 gaaaactaaa ccatacacca ccaacacaac caaacccacc acgcccaatt gttacacacc
       61 cgcttgaaaa agaaagtcta acaaatggcc aaagtgcgtg aggtttacca atcctttaca
      121 gactccacca caaaaactct cattcaagat gaggcctata gaaatattcg tcccatcatg
      181 gaaaaacaca aactatctaa cccgtacgct caaacggttg aagcggctaa tgatctagag
      241 gggttcggca tagccaccaa tccctatagc attgaattgc atacacatgc agccgctaag
      301 accatagaga ataaacttct agaggtgctt ggttccatcc taccacaaga acctgttaca
      361 tttatgttcc tcaaacctag gaagctaaac tacatgagaa gaaacccacg gattaaggac
      421 attttccaca atgttgccat tgaaccgaga gacgtggcaa ggtacccaaa ggaaacaata
      481 atagacaagc tcacagagat cacaacggac acagcataca ttagtgacac tctgcacttc
      541 ttggatccga gctacatagt ggagacattc caaaactgcc caaaattaca aaccttgtat
      601 gcgaccttag ttctccccgt tgaggccgcc ttcaaaatgg aaagcaccca cccgaacata
      661 tacagcctca aatactttgg agatggtttc cagtatatac caggcaacca tggtggtgga
      721 gcataccatc atgaatttgc tcatttacaa tggctcaaag tgggaaaaat caagtggagg
      781 gaccccaagg atagctttct cggacatctc aattacacga ctgagcaggt tgagatgcac
      841 acagtgacag tacagttgca ggaatcgttt gcggcaaacc acttgtactg catcaggaga
      901 ggagactggc tcacaccgga ggtacgcact ttcggacaac ctgacaggta tgtgattcca
      961 ccacagatct ttctcccgaa agttcacaac tgcaagaagc cgattctgaa gaaaactatg
     1021 atgcagctct tcttgtacgt taggacagtc aaggtcgcaa aaaattgtga catttttgcc
     1081 aaagtcagac aattaattaa atcgtctgac ttggacaaat actctgctgt ggaactggtt
     1141 tacttagtga gctatatgga gttccttgct gatctacaag ccaccacttg cttctcagac
     1201 acactttccg gtggcttgct aacaaagacc cttgcaccag tgagggcttg gatacaagag
     1261 aaaaagatgc aactgtttgg tcttgaggac tacgcgaagt tagtcaaagc agttgatttc
     1321 cacccagtgg acttttcttt taaagtggaa acttgggact tcagattcca tcccttgcaa
     1381 gcatggaagg ccttccgacc aagggaagtg tcggacgtag aggaaatgga aagtttgttc
     1441 gcagatgggg acctgcttga ttgcttcaca agaatgccag cttatgcagt gaacgcagag
     1501 gaagatttag ctgcaatcag gaaaacgcct gagatggatg tcggtcaagg aattaaagaa
     1561 cctgcaggag acagaaatca atactcaaac cctgcggaaa ccttcctcaa caagctccac
     1621 aggaaacaca gtagagaggt gaaacatcag accacaaaga aggctaagcg cctagctgaa
     1681 atccaggagt ccatgagagc agaaggtgag gctgaatcaa atgagatgag cgggggcatg
     1741 ggggcaatac cgagcaacgc tgaacttccc agcacgagtg atgctagaca agaactcaca
     1801 ctcccaacca ccaaatctgt tcccgcaagg tgggaagatg cttcactcac agattccagt
     1861 gtggaagagg agcaggtgaa gctccctgga aaagaagctg ttgagacagc aatgcaacaa
     1921 gtcatcgaag gactcccttg gaaacactgg attcctcaac taaacgctgt tggattcaag
     1981 gcgctagaaa ttcagaggga taggaatgga acaatgatca tgcctatcac agaaatggtc
     2041 tccgggttgg aaaaagagga cttcccggaa ggaactccaa aagagttggc acgagaattg
     2101 ctcgctatga acagaagtcc tgccaccatc cctttggacc tacttagagc cagagactac
     2161 ggcagtgatg tgaagaacaa gagaattggt gccatcacaa agacacaagc aacaagttgg
     2221 ggcgagtact taacaggaaa gatagaaagc ctgactgaga ggaaagttgc aacctgtgtc
     2281 attcatggag ctggaggctc tggaaaaagt catgccatcc agaaggcatt gagagaaatt
     2341 ggtaagggct cagacatcac cgtagtcctg ccaaccaatg aactgcgact agattggagt
     2401 aagaaagtgc ccaacactga accatacatg ttcaagacct atgaaaaggc attaattggg
     2461 ggaacaggca gtatagtcat ctttgatgat tactcaaaac ttcctcccgg ttacatagaa
     2521 gccttaatct gtttctactc caaaatcaag ctagtcattc taacaggaga tagcagacag
     2581 agcgtctacc atgaaactgc tgaggacgcc tccatcaggc atttgggacc agcgacagag
     2641 tacttctcaa aatactgccg atactatctc aatgccacac accgcaacaa gaaagacctt
     2701 gcgaacatgc ttggcgtcta cagtgagaga acaggggtta cagaaatcag catgagcgcc
     2761 gagtttttag aaggaatccc aactttagtg ccctcggatg aaaagagaaa gctgtacatg
     2821 ggcaccggaa ggaatgacac gttcacatac gctggatgcc aagggctaac caagccgaaa
     2881 gtacaaattg tgttggacca caacacccaa gtgtgtagtg cgaatgtgat gtacacggca
     2941 ctctctagag ctaccgacag gattcacttc gtgaacacaa gtgcaaactc ttcggccttc
     3001 tgggaaaagt tagacagcac cccttatctc aagactttcc tatcagtggt gagagaacaa
     3061 gcactcaagg agtacgagcc ggcagaggta gagccaattc gagagcctga gccccaaaca
     3121 cacatgtgtg tcgagaatga ggagtctgtg ctagaagagt acaaagagga actttttgaa
     3181 aagtttgaca gagagatcca ctctgaatcc catggccatt caaactgtgt ccaaactgaa
     3241 gacacaacca tccagctgtt ttcgcatcaa caagcaaagg atgagaccct cctctgggcg
     3301 accatagatg cgcggctcaa gaccagcaat caagaaacaa acttccgaga attcttgagc
     3361 aagaaggaca ttggagacgt cctgtttcta aactaccaga aagctatggg tttgcccaaa
     3421 gagcggattc ctttttcaca agaggtctgg gaagcttgtg cccatgaagt gcaaagcaaa
     3481 tacctcagta agtcaaagtg caacttgatt aatgggactg tgagacagag cccagacttt
     3541 gatgaaaaca aaatcatggt attcctcaag tcgcagtggg tcacaaaggt ggaaaagctg
     3601 ggtctaccca agattaagcc aggtcaaacc atagcagctt tttatcagca gactgtgatg
     3661 ctttttggaa ctatggctag gtacatgcga tggttcagac aggctttcca gccaaaagaa
     3721 gtcttcataa actgtgaaac cacgccagaa gacatgtctg catgggcctt gaacaactgg
     3781 aattttggca ggcctagttt ggctaatgac tatacggctt tcgaccaatc tcaggatgga
     3841 gctatgctgc aatttgaggt actcaaagcc aaacaccact gcataccaga ggaaatcatc
     3901 caagcataca tagacatcaa gaccaatgca cagattttcc taggcacatt gtcaattatg
     3961 cgcctgactg gtgaaggtcc cacttttgat gcaaacactg agtgcaacat agcttacacc
     4021 catacaaagt ttgacatccc agccggaact gcccaagttt atgcaggaga cgactcagca
     4081 ctggattgcg ttccagaagt gaagcctagt ttccacaggc ttgaagacaa attactcctc
     4141 aagtcgaagc ctgtaatcac gcagcaaaag aaaggcagtt ggcctgagtt ttgtggttgg
     4201 ctgattacac caaaaggggt aatgaaagac ccaatcaagc tccatgttag cttaaaattg
     4261 gccgaagcta agggcgaact taagaaatgt caagattcct atgaaattga tttgagttat
     4321 gcctatgacc acaaggactc tctgcatgac ttgttcgatg agaaacagtg tcaggcacat
     4381 acactcactt gcaggacact gattaagtca gggagaggca ctgtctcact tccccgcctc
     4441 agaaactttc tttaaccgtt aagttacctt agagatttga ataagatgga tattctcatc
     4501 attagtttga aaagtttagg ttattctagg acttctaaat ctttagattc aggacctttg
     4561 gtagtgcatg cagtagccgg agccggcaag tccaccgccc taaggaagtt gattctcaga
     4621 cacccaacat tcaccgtgca tacactcggt gtccctgaca aggtgagtat cagaactaga
     4681 ggcatacaga agccaggacc cattcctgag ggcaattttc caatcctcga tgagtatact
     4741 ttggacaaca ccacaaggga ctcataccag gcactttttg ctgaccctta tcaggcacct
     4801 gagtttagcc tagagcccca cttctacctg gaaacatcat ttcgagttcc gaggaaagtg
     4861 gcagatttga tagcaggttg cggcttcgat ttcgagacca actcacagga agaagggcat
     4921 ttagagatta ctggcatatt caaagggccc ctacttggga aggtaatagc cattgacgag
     4981 gagtcagaga caacactgtc caggcatggt gttgagtttg ttaagccctg ccaagtgact
     5041 ggacttgagt tcaaagtagt cactattgtg tctgccgcac caatagagga aattggccag
     5101 tccacagctt tttacaacgc catcaccagg tcaaagggat tgacatatgt ccgcgcaggg
     5161 acatagactg accgctccgg tcaattctga aaaagtgtac atagtattag gtctatcgtt
     5221 tgctttagtt tcaattactt tcctgctttc tagaagtaat ttaccccacg ttggtgacaa
     5281 cattcacagc ttgccacacg gaggagctta cagagacggc accaaagcaa tcttgtacaa
     5341 ctccccaaat ctagggtcac gagtgagtct acacaacgga aagaacgcag catttgctgc
     5401 cgttttgcta ctgactttgc tgatctatgg aagtaaatac atatctcaac gcaatcatac
     5461 ttgtgcttgt ggtaacaatc atagcagtca ttagtacctc cttagtgagg acggaacctt
     5521 gtgtcattaa gattactgga gaatcaatca cagtgttggc ttgcaaatta gacgcagaaa
     5581 ccatcagagc cattgccgat ctcaagccac tctccgttga acggttaagt ttccattgat
     5641 actcgaaaga ggtcgtcatc agcaagcgcg atcgccgccg ataagcttga tgaaaaaaga
     5701 gtgatgtccg taaagggaaa attagtagta gtgatcggtc agtgccgaac aagaactata
     5761 gaaatgtaaa ggattttgga ggaatgagtt ttaaaaagaa taatttaatc gatgatgatt
     5821 cggaggctac tgtcgccgaa tcggattcgt tttaaatagg tcttacagta tcactactcc
     5881 atctcagttc gtgttcttgt cagagctcgg tccggaggtt cgaacgacgc gtagagccat
     5941 tgccgatctc aagccactct ccgttgaacg gtaaagtttc cattgatact cgaaagatgt
     6001 cgtcatcagc tagcaaactc gagttttcgc gactacgtct acataaccga cgcctacccc
     6061 agtttcatag tattttctgg tttgattgta tgaataatat aaataaaaaa aaaaaaaaaa
     6121 aaaaaaaaaa aaacccggga attctaagag gagtccacca tggtagatct gactagtgtt
     6181 aacgcaagcc accaccacca ccaccacgtg tgaattacag gtgaccagct cgaatttccc
     6241 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     6301 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     6361 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     6421 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     6481 tatgttacta gatcgggaat taaactatca gtgtttgaca ggatatattg gcgggtaaac
     6541 ctaagagaaa agagcgttta ttagaataac ggatatttaa aagggcgtga aaaggtttat
     6601 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     6661 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     6721 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     6781 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     6841 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     6901 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     6961 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     7021 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     7081 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     7141 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     7201 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     7261 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     7321 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     7381 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     7441 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     7501 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     7561 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     7621 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     7681 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
     7741 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     7801 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     7861 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     7921 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     7981 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     8041 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     8101 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     8161 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     8221 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     8281 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     8341 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     8401 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     8461 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     8521 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     8581 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     8641 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     8701 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     8761 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     8821 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc
     8881 aagcccgagg aatcggcgtg acggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     8941 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
     9001 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
     9061 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     9121 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     9181 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     9241 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
     9301 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     9361 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     9421 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     9481 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     9541 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     9601 cgggcggccg gagtacatcg agatcgagca agctgattgg atgtaccgcg agatcacaga
     9661 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     9721 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     9781 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     9841 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     9901 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     9961 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
    10021 aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
    10081 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
    10141 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
    10201 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
    10261 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
    10321 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
    10381 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
    10441 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    10501 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    10561 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
    10621 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    10681 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
    10741 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    10801 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    10861 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    10921 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    10981 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    11041 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    11101 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    11161 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    11221 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    11281 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    11341 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    11401 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    11461 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    11521 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta ggtactaaaa
    11581 caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa
    11641 gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca
    11701 gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact
    11761 tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag
    11821 ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg
    11881 agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc
    11941 caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga
    12001 gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc
    12061 ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc
    12121 atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat
    12181 catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt
    12241 taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc
    12301 cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat
    12361 tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
    12421 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
    12481 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
    12541 attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct
    12601 accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata
    12661 gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc
    12721 ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
    12781 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
    12841 ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt cctaggccac
    12901 catgttgggc ccggcgcgcc aagcttggct gcaggtcgac ctcgcatgcc tgcaggtcaa
    12961 catggtggag cacgacacgc ttgtctactc caaaaatatc aaagatacag tctcagaaga
    13021 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
    13081 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
    13141 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
    13201 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
    13261 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
    13321 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agagga
//
