LOCUS       V008725                14054 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008725
VERSION     V008725
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14054)
  AUTHORS   Wang Y, Cong QQ, Lan YF, Geng C, Li XD, Liang YC, Yang ZY, Zhu XP,
            Li XD.
  TITLE     Development of new potato virus X-based vectors for gene
            over-expression and gene silencing assay
  JOURNAL   Virus Res. 191, 62-69 (2014)
   PUBMED   25076104
REFERENCE   2  (bases 1 to 14054)
  AUTHORS   Wang Y, Cong QQ, Li XD.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2014) Department of Plant Pathology, Shandong
            Agricultural University, No. 61 Daizong street, Tai'an, Shandong
            Province 271018, PR China
REFERENCE   3  (bases 1 to 14054)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14054)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res.
            191, 62-69 (2014)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-APR-2014) Department of Plant Pathology, Shandong Agricultural
            University, No. 61 Daizong street, Tai'an, Shandong Province 271018,
            PR China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           1..84
                     /label="derived from PVX isolate 1985"
                     /note="derived from PVX isolate 1985"
     CDS             85..4452
                     /note="RNA replication protein from Potato virus X.
                     Accession#: P09395"
                     /label="RNA replication protein"
     CDS             4486..5163
                     /note="Movement and silencing protein TGBp1 from Potato
                     virus X (strain X3). Accession#: P17780"
                     /label="Movement and silencing protein TGBp1"
     CDS             5147..5494
                     /codon_start=1
                     /gene="TGB2"
                     /product="triple gene block protein 2"
                     /label="TGB2"
                     /protein_id="AII77187.1"
                     /translation="MSAQGHRLTAPVNSEKVYIVLGLSFALVSITFLLSRSNLPHVGDN
                     IHSLPHGGAYRDGTKAILYNSPNLGSRVSLHNGKNAAFAAVLLLTLLIYGSKYISQRNH
                     TCACGNNHSSH"
     gene            5147..5494
                     /gene="TGB2"
                     /label="TGB2"
                     /note="derived from PVX isolate 1985"
     CDS             5427..5639
                     /codon_start=1
                     /gene="TGB3"
                     /product="triple gene block protein 3"
                     /label="TGB3"
                     /protein_id="AII77189.1"
                     /translation="MEVNTYLNAIILVLVVTIIAVISTSLVRTEPCVIKITGESITVLA
                     CKLDAETIRAIADLKPLSVERLSFH"
     gene            5427..5639
                     /gene="TGB3"
                     /label="TGB3"
                     /note="derived from PVX isolate 1985"
     RBS             5496..5504
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    5668..5691
                     /label="cloning site"
                     /note="cloning site"
     regulatory      5692..5902
                     /note="derived from tobacco mosaic virus coat protein
                     subgenomic promoter"
                     /regulatory_class="promoter"
     misc_feature    5903..5932
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      5933..6014
                     /label="derived from PVX isolate 1985"
                     /note="derived from PVX isolate 1985"
                     /regulatory_class="promoter"
     CDS             5997..6710
                     /codon_start=1
                     /gene="cp"
                     /product="coat protein"
                     /label="cp"
                     /protein_id="AII77190.1"
                     /translation="MSSSASTTQATGSTTSTTTKTAGATPATASGLFTIPDGDFFSTAR
                     AIVASNAVATNEDLSKIEAIWKDMKVPTDTMAQAAWDLVRHCADVGSSAQTEMIDTGPY
                     SNGISRARLAAAIKEVCTLRQFCMKYAPVVWNWMLTNNSPPANWQAQGFKPEHKFAAFD
                     FFNGVTNPAAIMPKEGLIRPPSEAEMNAAQTAAFVKITKARAQSNDFASLDAAVTRGRI
                     TGTTTAEAVVTLPPP"
     gene            5997..6710
                     /gene="cp"
                     /label="cp"
                     /note="derived from PVX isolate 1985"
     3'UTR           6711..6811
                     /label="derived from PVX isolate 1985"
                     /note="derived from PVX isolate 1985"
     CDS             6868..6885
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      6920..7172
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7194..7218
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8518..9144
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             9581..10645
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      10714..10908
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    11252..11392
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11578..12166)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12256..13047)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    13472..13496
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        13711..14054
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 gaaaactaaa ccatacacca ccaacacaac caaacccacc acgcccaatt gttacacacc
       61 cgcttgaaaa agaaagtcta acaaatggcc aaagtgcgtg aggtttacca atcctttaca
      121 gactccacca caaaaactct cattcaagat gaggcctata gaaatattcg tcccatcatg
      181 gaaaaacaca aactatctaa cccgtacgct caaacggttg aagcggctaa tgatctagag
      241 gggttcggca tagccaccaa tccctatagc attgaattgc atacacatgc agccgctaag
      301 accatagaga ataaacttct agaggtgctt ggttccatcc taccacaaga acctgttaca
      361 tttatgttcc tcaaacctag gaagctaaac tacatgagaa gaaacccacg gattaaggac
      421 attttccaca atgttgccat tgaaccgaga gacgtggcaa ggtacccaaa ggaaacaata
      481 atagacaagc tcacagagat cacaacggac acagcataca ttagtgacac tctgcacttc
      541 ttggatccga gctacatagt ggagacattc caaaactgcc caaaattaca aaccttgtat
      601 gcgaccttag ttctccccgt tgaggccgcc ttcaaaatgg aaagcaccca cccgaacata
      661 tacagcctca aatactttgg agatggtttc cagtatatac caggcaacca tggtggtgga
      721 gcataccatc atgaatttgc tcatttacaa tggctcaaag tgggaaaaat caagtggagg
      781 gaccccaagg atagctttct cggacatctc aattacacga ctgagcaggt tgagatgcac
      841 acagtgacag tacagttgca ggaatcgttt gcggcaaacc acttgtactg catcaggaga
      901 ggagactggc tcacaccgga ggtacgcact ttcggacaac ctgacaggta tgtgattcca
      961 ccacagatct ttctcccgaa agttcacaac tgcaagaagc cgattctgaa gaaaactatg
     1021 atgcagctct tcttgtacgt taggacagtc aaggtcgcaa aaaattgtga catttttgcc
     1081 aaagtcagac aattaattaa atcgtctgac ttggacaaat actctgctgt ggaactggtt
     1141 tacttagtga gctatatgga gttccttgct gatctacaag ccaccacttg cttctcagac
     1201 acactttccg gtggcttgct aacaaagacc cttgcaccag tgagggcttg gatacaagag
     1261 aaaaagatgc aactgtttgg tcttgaggac tacgcgaagt tagtcaaagc agttgatttc
     1321 cacccagtgg acttttcttt taaagtggaa acttgggact tcagattcca tcccttgcaa
     1381 gcatggaagg ccttccgacc aagggaagtg tcggacgtag aggaaatgga aagtttgttc
     1441 gcagatgggg acctgcttga ttgcttcaca agaatgccag cttatgcagt gaacgcagag
     1501 gaagatttag ctgcaatcag gaaaacgcct gagatggatg tcggtcaagg aattaaagaa
     1561 cctgcaggag acagaaatca atactcaaac cctgcggaaa ccttcctcaa caagctccac
     1621 aggaaacaca gtagagaggt gaaacatcag accacaaaga aggctaagcg cctagctgaa
     1681 atccaggagt ccatgagagc agaaggtgag gctgaatcaa atgagatgag cgggggcatg
     1741 ggggcaatac cgagcaacgc tgaacttccc agcacgagtg atgctagaca agaactcaca
     1801 ctcccaacca ccaaatctgt tcccgcaagg tgggaagatg cttcactcac agattccagt
     1861 gtggaagagg agcaggtgaa gctccctgga aaagaagctg ttgagacagc aatgcaacaa
     1921 gtcatcgaag gactcccttg gaaacactgg attcctcaac taaacgctgt tggattcaag
     1981 gcgctagaaa ttcagaggga taggaatgga acaatgatca tgcctatcac agaaatggtc
     2041 tccgggttgg aaaaagagga cttcccggaa ggaactccaa aagagttggc acgagaattg
     2101 ctcgctatga acagaagtcc tgccaccatc cctttggacc tacttagagc cagagactac
     2161 ggcagtgatg tgaagaacaa gagaattggt gccatcacaa agacacaagc aacaagttgg
     2221 ggcgagtact taacaggaaa gatagaaagc ctgactgaga ggaaagttgc aacctgtgtc
     2281 attcatggag ctggaggctc tggaaaaagt catgccatcc agaaggcatt gagagaaatt
     2341 ggtaagggct cagacatcac cgtagtcctg ccaaccaatg aactgcgact agattggagt
     2401 aagaaagtgc ccaacactga accatacatg ttcaagacct atgaaaaggc attaattggg
     2461 ggaacaggca gtatagtcat ctttgatgat tactcaaaac ttcctcccgg ttacatagaa
     2521 gccttaatct gtttctactc caaaatcaag ctagtcattc taacaggaga tagcagacag
     2581 agcgtctacc atgaaactgc tgaggacgcc tccatcaggc atttgggacc agcgacagag
     2641 tacttctcaa aatactgccg atactatctc aatgccacac accgcaacaa gaaagacctt
     2701 gcgaacatgc ttggcgtcta cagtgagaga acaggggtta cagaaatcag catgagcgcc
     2761 gagtttttag aaggaatccc aactttagtg ccctcggatg aaaagagaaa gctgtacatg
     2821 ggcaccggaa ggaatgacac gttcacatac gctggatgcc aagggctaac caagccgaaa
     2881 gtacaaattg tgttggacca caacacccaa gtgtgtagtg cgaatgtgat gtacacggca
     2941 ctctctagag ctaccgacag gattcacttc gtgaacacaa gtgcaaactc ttcggccttc
     3001 tgggaaaagt tagacagcac cccttatctc aagactttcc tatcagtggt gagagaacaa
     3061 gcactcaagg agtacgagcc ggcagaggta gagccaattc gagagcctga gccccaaaca
     3121 cacatgtgtg tcgagaatga ggagtctgtg ctagaagagt acaaagagga actttttgaa
     3181 aagtttgaca gagagatcca ctctgaatcc catggccatt caaactgtgt ccaaactgaa
     3241 gacacaacca tccagctgtt ttcgcatcaa caagcaaagg atgagaccct cctctgggcg
     3301 accatagatg cgcggctcaa gaccagcaat caagaaacaa acttccgaga attcttgagc
     3361 aagaaggaca ttggagacgt cctgtttcta aactaccaga aagctatggg tttgcccaaa
     3421 gagcggattc ctttttcaca agaggtctgg gaagcttgtg cccatgaagt gcaaagcaaa
     3481 tacctcagta agtcaaagtg caacttgatt aatgggactg tgagacagag cccagacttt
     3541 gatgaaaaca aaatcatggt attcctcaag tcgcagtggg tcacaaaggt ggaaaagctg
     3601 ggtctaccca agattaagcc aggtcaaacc atagcagctt tttatcagca gactgtgatg
     3661 ctttttggaa ctatggctag gtacatgcga tggttcagac aggctttcca gccaaaagaa
     3721 gtcttcataa actgtgaaac cacgccagaa gacatgtctg catgggcctt gaacaactgg
     3781 aattttggca ggcctagttt ggctaatgac tatacggctt tcgaccaatc tcaggatgga
     3841 gctatgctgc aatttgaggt actcaaagcc aaacaccact gcataccaga ggaaatcatc
     3901 caagcataca tagacatcaa gaccaatgca cagattttcc taggcacatt gtcaattatg
     3961 cgcctgactg gtgaaggtcc cacttttgat gcaaacactg agtgcaacat agcttacacc
     4021 catacaaagt ttgacatccc agccggaact gcccaagttt atgcaggaga cgactcagca
     4081 ctggattgcg ttccagaagt gaagcctagt ttccacaggc ttgaagacaa attactcctc
     4141 aagtcgaagc ctgtaatcac gcagcaaaag aaaggcagtt ggcctgagtt ttgtggttgg
     4201 ctgattacac caaaaggggt aatgaaagac ccaatcaagc tccatgttag cttaaaattg
     4261 gccgaagcta agggcgaact taagaaatgt caagattcct atgaaattga tttgagttat
     4321 gcctatgacc acaaggactc tctgcatgac ttgttcgatg agaaacagtg tcaggcacat
     4381 acactcactt gcaggacact gattaagtca gggagaggca ctgtctcact tccccgcctc
     4441 agaaactttc tttaaccgtt aagttacctt agagatttga ataagatgga tattctcatc
     4501 attagtttga aaagtttagg ttattctagg acttctaaat ctttagattc aggacctttg
     4561 gtagtgcatg cagtagccgg agccggcaag tccaccgccc taaggaagtt gattctcaga
     4621 cacccaacat tcaccgtgca tacactcggt gtccctgaca aggtgagtat cagaactaga
     4681 ggcatacaga agccaggacc cattcctgag ggcaattttc caatcctcga tgagtatact
     4741 ttggacaaca ccacaaggga ctcataccag gcactttttg ctgaccctta tcaggcacct
     4801 gagtttagcc tagagcccca cttctacctg gaaacatcat ttcgagttcc gaggaaagtg
     4861 gcagatttga tagcaggttg cggcttcgat ttcgagacca actcacagga agaagggcat
     4921 ttagagatta ctggcatatt caaagggccc ctacttggga aggtaatagc cattgacgag
     4981 gagtcagaga caacactgtc caggcatggt gttgagtttg ttaagccctg ccaagtgact
     5041 ggacttgagt tcaaagtagt cactattgtg tctgccgcac caatagagga aattggccag
     5101 tccacagctt tttacaacgc catcaccagg tcaaagggat tgacatatgt ccgcgcaggg
     5161 acatagactg accgctccgg tcaattctga aaaagtgtac atagtattag gtctatcgtt
     5221 tgctttagtt tcaattactt tcctgctttc tagaagtaat ttaccccacg ttggtgacaa
     5281 cattcacagc ttgccacacg gaggagctta cagagacggc accaaagcaa tcttgtacaa
     5341 ctccccaaat ctagggtcac gagtgagtct acacaacgga aagaacgcag catttgctgc
     5401 cgttttgcta ctgactttgc tgatctatgg aagtaaatac atatctcaac gcaatcatac
     5461 ttgtgcttgt ggtaacaatc atagcagtca ttagtacctc cttagtgagg acggaacctt
     5521 gtgtcattaa gattactgga gaatcaatca cagtgttggc ttgcaaatta gacgcagaaa
     5581 ccatcagagc cattgccgat ctcaagccac tctccgttga acggttaagt ttccattgat
     5641 actcgaaaga ggtcgtcatc agctagcgcg atcgccgccg ataagcttga tgaaaaaaga
     5701 gtgatgtccg taaagggaaa attagtagta gtgatcggtc agtgccgaac aagaactata
     5761 gaaatgtaaa ggattttgga ggaatgagtt ttaaaaagaa taatttaatc gatgatgatt
     5821 cggaggctac tgtcgccgaa tcggattcgt tttaaatagg tcttacagta tcactactcc
     5881 atctcagttc gtgttcttgt cagagctcgg tccggaggtt cgaacgacgc gtagagccat
     5941 tgccgatctc aagccactct ccgttgaacg gtaaagtttc cattgatact cgaaagatgt
     6001 cgtcatcagc tagcacaaca caggccacag ggtcaactac ctcaactacc acaaaaactg
     6061 caggcgcaac tcctgccaca gcttcaggac tgttcaccat cccggatggg gatttcttta
     6121 gcacagcccg tgccatagta gccagcaatg ccgttgcaac aaatgaggac ctcagcaaga
     6181 ttgaggctat ctggaaggac atgaaggtgc ccacagacac tatggcacag gctgcttggg
     6241 acttagtcag acactgtgct gatgtgggct catctgccca aacagagatg atagatacag
     6301 gtccttattc caatggcatc agtagagcta gattggcagc agcgattaaa gaggtgtgca
     6361 cacttaggca attctgcatg aagtatgccc cagtggtatg gaactggatg ttgactaaca
     6421 acagtccacc tgctaactgg caagcgcaag gtttcaagcc tgagcacaaa ttcgctgcat
     6481 tcgacttctt caacggagtc accaacccag ctgccatcat gcccaaagag ggactcatcc
     6541 ggccaccgtc tgaagctgaa atgaatgctg cccaaactgc tgcctttgtg aagattacga
     6601 aggccagggc acaatccaac gactttgcca gcctagatgc agctgtcact cgaggtcgta
     6661 ttactggaac aacaaccgct gaggctgttg tcactctccc accaccataa ctacgtctac
     6721 ataaccgacg cctaccccag tttcatagta ttttctggtt tgattgtatg aataatataa
     6781 ataaaaaaaa aaaaaaaaaa aaaaaaaaaa acccgggaat tctaagagga gtccaccatg
     6841 gtagatctga ctagtgttaa cgctagccac caccaccacc accacgtgtg aattacaggt
     6901 gaccagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     6961 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     7021 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     7081 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     7141 atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta aactatcagt gtttgacagg
     7201 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataacgg atatttaaaa
     7261 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
     7321 ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac
     7381 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg
     7441 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga
     7501 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc
     7561 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc
     7621 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc
     7681 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc
     7741 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg
     7801 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc
     7861 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc
     7921 gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta
     7981 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg
     8041 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc
     8101 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat
     8161 tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa
     8221 accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat
     8281 gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga
     8341 aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga
     8401 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca
     8461 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg
     8521 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat
     8581 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag
     8641 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc
     8701 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc
     8761 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg
     8821 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg
     8881 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg
     8941 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg
     9001 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc
     9061 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc
     9121 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca
     9181 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca
     9241 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca
     9301 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat
     9361 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg
     9421 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc
     9481 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc
     9541 aatggcactg gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc
     9601 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
     9661 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
     9721 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
     9781 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
     9841 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
     9901 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
     9961 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
    10021 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
    10081 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
    10141 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
    10201 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
    10261 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
    10321 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
    10381 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
    10441 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
    10501 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
    10561 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
    10621 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
    10681 agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc
    10741 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
    10801 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
    10861 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
    10921 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
    10981 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
    11041 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca
    11101 tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    11161 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    11221 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
    11281 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
    11341 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
    11401 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    11461 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    11521 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    11581 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    11641 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    11701 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    11761 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    11821 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    11881 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    11941 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    12001 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    12061 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    12121 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    12181 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    12241 gcattctagg tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc
    12301 aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc
    12361 cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc
    12421 aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc
    12481 gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc
    12541 gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc
    12601 gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca
    12661 atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt
    12721 ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat
    12781 gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt
    12841 tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata
    12901 ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt
    12961 agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa
    13021 ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct acagtattta
    13081 aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc
    13141 taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac
    13201 atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat
    13261 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
    13321 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
    13381 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
    13441 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
    13501 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg
    13561 aattaattcc taggccacca tgttgggccc ggcgcgccaa gcttggctgc aggtcgacct
    13621 cgcatgcctg caggtcaaca tggtggagca cgacacgctt gtctactcca aaaatatcaa
    13681 agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg
    13741 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa
    13801 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc
    13861 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
    13921 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
    13981 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
    14041 tcatttggag agga
//
