LOCUS       V008726                13707 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008726
VERSION     V008726
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13707)
  AUTHORS   Wang Y, Cong QQ, Lan YF, Geng C, Li XD, Liang YC, Yang ZY, Zhu XP,
            Li XD.
  TITLE     Development of new potato virus X-based vectors for gene
            over-expression and gene silencing assay
  JOURNAL   Virus Res. 191, 62-69 (2014)
   PUBMED   25076104
REFERENCE   2  (bases 1 to 13707)
  AUTHORS   Wang Y, Cong QQ, Li XD.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2014) Department of Plant Pathology, Shandong
            Agricultural University, No. 61 Daizong street, Tai'an, Shandong
            Province 271018, PR China
REFERENCE   3  (bases 1 to 13707)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13707)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res.
            191, 62-69 (2014)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-APR-2014) Department of Plant Pathology, Shandong Agricultural
            University, No. 61 Daizong street, Tai'an, Shandong Province 271018,
            PR China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           1..84
                     /label="derived from PVX isolate 1985"
                     /note="derived from PVX isolate 1985"
     CDS             85..4452
                     /note="RNA replication protein from Potato virus X.
                     Accession#: P09395"
                     /label="RNA replication protein"
     CDS             4486..5163
                     /note="Movement and silencing protein TGBp1 from Potato
                     virus X (strain X3). Accession#: P17780"
                     /label="Movement and silencing protein TGBp1"
     CDS             5147..5494
                     /codon_start=1
                     /gene="TGB2"
                     /product="triple gene block protein 2"
                     /label="TGB2"
                     /protein_id="AII77183.1"
                     /translation="MSAQGHRLTAPVNSEKVYIVLGLSFALVSITFLLSRSNLPHVGDN
                     IHSLPHGGAYRDGTKAILYNSPNLGSRVSLHNGKNAAFAAVLLLTLLIYGSKYISQRNH
                     TCACGNNHSSH"
     gene            5147..5494
                     /gene="TGB2"
                     /label="TGB2"
                     /note="derived from PVX isolate 1985"
     CDS             5427..5639
                     /codon_start=1
                     /gene="TGB3"
                     /product="triple gene block protein 3"
                     /label="TGB3"
                     /protein_id="AII77184.1"
                     /translation="MEVNTYLNAIILVLVVTIIAVISTSLVRTEPCVIKITGESITVLA
                     CKLDAETIRAIADLKPLSVERLSFH"
     gene            5427..5639
                     /gene="TGB3"
                     /label="TGB3"
                     /note="derived from PVX isolate 1985"
     RBS             5496..5504
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5650..6363
                     /codon_start=1
                     /gene="cp"
                     /product="coat protein"
                     /label="cp"
                     /protein_id="AII77185.1"
                     /translation="MSSSASTTQATGSTTSTTTKTAGATPATASGLFTIPDGDFFSTAR
                     AIVASNAVATNEDLSKIEAIWKDMKVPTDTMAQAAWDLVRHCADVGSSAQTEMIDTGPY
                     SNGISRARLAAAIKEVCTLRQFCMKYAPVVWNWMLTNNSPPANWQAQGFKPEHKFAAFD
                     FFNGVTNPAAIMPKEGLIRPPSEAEMNAAQTAAFVKITKARAQSNDFASLDAAVTRGRI
                     TGTTTAEAVVTLPPP"
     gene            5650..6363
                     /gene="cp"
                     /label="cp"
                     /note="derived from PVX isolate 1985"
     3'UTR           6364..6464
                     /label="derived from PVX isolate 1985"
                     /note="derived from PVX isolate 1985"
     CDS             6521..6538
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      6573..6825
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6847..6871
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8171..8797
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             9234..10298
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      10367..10561
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10905..11045
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11231..11819)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(11909..12700)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    13125..13149
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        13364..13707
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 gaaaactaaa ccatacacca ccaacacaac caaacccacc acgcccaatt gttacacacc
       61 cgcttgaaaa agaaagtcta acaaatggcc aaagtgcgtg aggtttacca atcctttaca
      121 gactccacca caaaaactct cattcaagat gaggcctata gaaatattcg tcccatcatg
      181 gaaaaacaca aactatctaa cccgtacgct caaacggttg aagcggctaa tgatctagag
      241 gggttcggca tagccaccaa tccctatagc attgaattgc atacacatgc agccgctaag
      301 accatagaga ataaacttct agaggtgctt ggttccatcc taccacaaga acctgttaca
      361 tttatgttcc tcaaacctag gaagctaaac tacatgagaa gaaacccacg gattaaggac
      421 attttccaca atgttgccat tgaaccgaga gacgtggcaa ggtacccaaa ggaaacaata
      481 atagacaagc tcacagagat cacaacggac acagcataca ttagtgacac tctgcacttc
      541 ttggatccga gctacatagt ggagacattc caaaactgcc caaaattaca aaccttgtat
      601 gcgaccttag ttctccccgt tgaggccgcc ttcaaaatgg aaagcaccca cccgaacata
      661 tacagcctca aatactttgg agatggtttc cagtatatac caggcaacca tggtggtgga
      721 gcataccatc atgaatttgc tcatttacaa tggctcaaag tgggaaaaat caagtggagg
      781 gaccccaagg atagctttct cggacatctc aattacacga ctgagcaggt tgagatgcac
      841 acagtgacag tacagttgca ggaatcgttt gcggcaaacc acttgtactg catcaggaga
      901 ggagactggc tcacaccgga ggtacgcact ttcggacaac ctgacaggta tgtgattcca
      961 ccacagatct ttctcccgaa agttcacaac tgcaagaagc cgattctgaa gaaaactatg
     1021 atgcagctct tcttgtacgt taggacagtc aaggtcgcaa aaaattgtga catttttgcc
     1081 aaagtcagac aattaattaa atcgtctgac ttggacaaat actctgctgt ggaactggtt
     1141 tacttagtga gctatatgga gttccttgct gatctacaag ccaccacttg cttctcagac
     1201 acactttccg gtggcttgct aacaaagacc cttgcaccag tgagggcttg gatacaagag
     1261 aaaaagatgc aactgtttgg tcttgaggac tacgcgaagt tagtcaaagc agttgatttc
     1321 cacccagtgg acttttcttt taaagtggaa acttgggact tcagattcca tcccttgcaa
     1381 gcatggaagg ccttccgacc aagggaagtg tcggacgtag aggaaatgga aagtttgttc
     1441 gcagatgggg acctgcttga ttgcttcaca agaatgccag cttatgcagt gaacgcagag
     1501 gaagatttag ctgcaatcag gaaaacgcct gagatggatg tcggtcaagg aattaaagaa
     1561 cctgcaggag acagaaatca atactcaaac cctgcggaaa ccttcctcaa caagctccac
     1621 aggaaacaca gtagagaggt gaaacatcag accacaaaga aggctaagcg cctagctgaa
     1681 atccaggagt ccatgagagc agaaggtgag gctgaatcaa atgagatgag cgggggcatg
     1741 ggggcaatac cgagcaacgc tgaacttccc agcacgagtg atgctagaca agaactcaca
     1801 ctcccaacca ccaaatctgt tcccgcaagg tgggaagatg cttcactcac agattccagt
     1861 gtggaagagg agcaggtgaa gctccctgga aaagaagctg ttgagacagc aatgcaacaa
     1921 gtcatcgaag gactcccttg gaaacactgg attcctcaac taaacgctgt tggattcaag
     1981 gcgctagaaa ttcagaggga taggaatgga acaatgatca tgcctatcac agaaatggtc
     2041 tccgggttgg aaaaagagga cttcccggaa ggaactccaa aagagttggc acgagaattg
     2101 ctcgctatga acagaagtcc tgccaccatc cctttggacc tacttagagc cagagactac
     2161 ggcagtgatg tgaagaacaa gagaattggt gccatcacaa agacacaagc aacaagttgg
     2221 ggcgagtact taacaggaaa gatagaaagc ctgactgaga ggaaagttgc aacctgtgtc
     2281 attcatggag ctggaggctc tggaaaaagt catgccatcc agaaggcatt gagagaaatt
     2341 ggtaagggct cagacatcac cgtagtcctg ccaaccaatg aactgcgact agattggagt
     2401 aagaaagtgc ccaacactga accatacatg ttcaagacct atgaaaaggc attaattggg
     2461 ggaacaggca gtatagtcat ctttgatgat tactcaaaac ttcctcccgg ttacatagaa
     2521 gccttaatct gtttctactc caaaatcaag ctagtcattc taacaggaga tagcagacag
     2581 agcgtctacc atgaaactgc tgaggacgcc tccatcaggc atttgggacc agcgacagag
     2641 tacttctcaa aatactgccg atactatctc aatgccacac accgcaacaa gaaagacctt
     2701 gcgaacatgc ttggcgtcta cagtgagaga acaggggtta cagaaatcag catgagcgcc
     2761 gagtttttag aaggaatccc aactttagtg ccctcggatg aaaagagaaa gctgtacatg
     2821 ggcaccggaa ggaatgacac gttcacatac gctggatgcc aagggctaac caagccgaaa
     2881 gtacaaattg tgttggacca caacacccaa gtgtgtagtg cgaatgtgat gtacacggca
     2941 ctctctagag ctaccgacag gattcacttc gtgaacacaa gtgcaaactc ttcggccttc
     3001 tgggaaaagt tagacagcac cccttatctc aagactttcc tatcagtggt gagagaacaa
     3061 gcactcaagg agtacgagcc ggcagaggta gagccaattc gagagcctga gccccaaaca
     3121 cacatgtgtg tcgagaatga ggagtctgtg ctagaagagt acaaagagga actttttgaa
     3181 aagtttgaca gagagatcca ctctgaatcc catggccatt caaactgtgt ccaaactgaa
     3241 gacacaacca tccagctgtt ttcgcatcaa caagcaaagg atgagaccct cctctgggcg
     3301 accatagatg cgcggctcaa gaccagcaat caagaaacaa acttccgaga attcttgagc
     3361 aagaaggaca ttggagacgt cctgtttcta aactaccaga aagctatggg tttgcccaaa
     3421 gagcggattc ctttttcaca agaggtctgg gaagcttgtg cccatgaagt gcaaagcaaa
     3481 tacctcagta agtcaaagtg caacttgatt aatgggactg tgagacagag cccagacttt
     3541 gatgaaaaca aaatcatggt attcctcaag tcgcagtggg tcacaaaggt ggaaaagctg
     3601 ggtctaccca agattaagcc aggtcaaacc atagcagctt tttatcagca gactgtgatg
     3661 ctttttggaa ctatggctag gtacatgcga tggttcagac aggctttcca gccaaaagaa
     3721 gtcttcataa actgtgaaac cacgccagaa gacatgtctg catgggcctt gaacaactgg
     3781 aattttggca ggcctagttt ggctaatgac tatacggctt tcgaccaatc tcaggatgga
     3841 gctatgctgc aatttgaggt actcaaagcc aaacaccact gcataccaga ggaaatcatc
     3901 caagcataca tagacatcaa gaccaatgca cagattttcc taggcacatt gtcaattatg
     3961 cgcctgactg gtgaaggtcc cacttttgat gcaaacactg agtgcaacat agcttacacc
     4021 catacaaagt ttgacatccc agccggaact gcccaagttt atgcaggaga cgactcagca
     4081 ctggattgcg ttccagaagt gaagcctagt ttccacaggc ttgaagacaa attactcctc
     4141 aagtcgaagc ctgtaatcac gcagcaaaag aaaggcagtt ggcctgagtt ttgtggttgg
     4201 ctgattacac caaaaggggt aatgaaagac ccaatcaagc tccatgttag cttaaaattg
     4261 gccgaagcta agggcgaact taagaaatgt caagattcct atgaaattga tttgagttat
     4321 gcctatgacc acaaggactc tctgcatgac ttgttcgatg agaaacagtg tcaggcacat
     4381 acactcactt gcaggacact gattaagtca gggagaggca ctgtctcact tccccgcctc
     4441 agaaactttc tttaaccgtt aagttacctt agagatttga ataagatgga tattctcatc
     4501 attagtttga aaagtttagg ttattctagg acttctaaat ctttagattc aggacctttg
     4561 gtagtgcatg cagtagccgg agccggcaag tccaccgccc taaggaagtt gattctcaga
     4621 cacccaacat tcaccgtgca tacactcggt gtccctgaca aggtgagtat cagaactaga
     4681 ggcatacaga agccaggacc cattcctgag ggcaattttc caatcctcga tgagtatact
     4741 ttggacaaca ccacaaggga ctcataccag gcactttttg ctgaccctta tcaggcacct
     4801 gagtttagcc tagagcccca cttctacctg gaaacatcat ttcgagttcc gaggaaagtg
     4861 gcagatttga tagcaggttg cggcttcgat ttcgagacca actcacagga agaagggcat
     4921 ttagagatta ctggcatatt caaagggccc ctacttggga aggtaatagc cattgacgag
     4981 gagtcagaga caacactgtc caggcatggt gttgagtttg ttaagccctg ccaagtgact
     5041 ggacttgagt tcaaagtagt cactattgtg tctgccgcac caatagagga aattggccag
     5101 tccacagctt tttacaacgc catcaccagg tcaaagggat tgacatatgt ccgcgcaggg
     5161 acatagactg accgctccgg tcaattctga aaaagtgtac atagtattag gtctatcgtt
     5221 tgctttagtt tcaattactt tcctgctttc tagaagtaat ttaccccacg ttggtgacaa
     5281 cattcacagc ttgccacacg gaggagctta cagagacggc accaaagcaa tcttgtacaa
     5341 ctccccaaat ctagggtcac gagtgagtct acacaacgga aagaacgcag catttgctgc
     5401 cgttttgcta ctgactttgc tgatctatgg aagtaaatac atatctcaac gcaatcatac
     5461 ttgtgcttgt ggtaacaatc atagcagtca ttagtacctc cttagtgagg acggaacctt
     5521 gtgtcattaa gattactgga gaatcaatca cagtgttggc ttgcaaatta gacgcagaaa
     5581 ccatcagagc cattgccgat ctcaagccac tctccgttga acggttaagt ttccattgat
     5641 actcgaaaga tgtcgtcatc agctagcaca acacaggcca cagggtcaac tacctcaact
     5701 accacaaaaa ctgcaggcgc aactcctgcc acagcttcag gactgttcac catcccggat
     5761 ggggatttct ttagcacagc ccgtgccata gtagccagca atgccgttgc aacaaatgag
     5821 gacctcagca agattgaggc tatctggaag gacatgaagg tgcccacaga cactatggca
     5881 caggctgctt gggacttagt cagacactgt gctgatgtgg gctcatctgc ccaaacagag
     5941 atgatagata caggtcctta ttccaatggc atcagtagag ctagattggc agcagcgatt
     6001 aaagaggtgt gcacacttag gcaattctgc atgaagtatg ccccagtggt atggaactgg
     6061 atgttgacta acaacagtcc acctgctaac tggcaagcgc aaggtttcaa gcctgagcac
     6121 aaattcgctg cattcgactt cttcaacgga gtcaccaacc cagctgccat catgcccaaa
     6181 gagggactca tccggccacc gtctgaagct gaaatgaatg ctgcccaaac tgctgccttt
     6241 gtgaagatta cgaaggccag ggcacaatcc aacgactttg ccagcctaga tgcagctgtc
     6301 actcgaggtc gtattactgg aacaacaacc gctgaggctg ttgtcactct cccaccacca
     6361 taactacgtc tacataaccg acgcctaccc cagtttcata gtattttctg gtttgattgt
     6421 atgaataata taaataaaaa aaaaaaaaaa aaaaaaaaaa aaaacccggg aattctaaga
     6481 ggagtccacc atggtagatc tgactagtgt taacgctagc caccaccacc accaccacgt
     6541 gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     6601 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     6661 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     6721 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     6781 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttaaactatc
     6841 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     6901 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     6961 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
     7021 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
     7081 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     7141 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     7201 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     7261 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     7321 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     7381 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     7441 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     7501 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     7561 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     7621 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     7681 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     7741 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     7801 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     7861 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     7921 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg
     7981 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     8041 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     8101 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
     8161 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     8221 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     8281 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     8341 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     8401 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     8461 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     8521 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     8581 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     8641 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     8701 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     8761 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     8821 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     8881 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     8941 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     9001 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     9061 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     9121 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt
     9181 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca
     9241 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     9301 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     9361 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     9421 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     9481 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     9541 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     9601 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     9661 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     9721 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     9781 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     9841 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     9901 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     9961 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
    10021 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
    10081 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
    10141 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
    10201 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
    10261 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
    10321 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
    10381 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
    10441 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
    10501 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
    10561 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
    10621 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
    10681 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
    10741 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
    10801 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    10861 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
    10921 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
    10981 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    11041 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    11101 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    11161 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    11221 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    11281 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    11341 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    11401 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    11461 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    11521 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    11581 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    11641 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    11701 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    11761 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    11821 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    11881 ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta
    11941 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
    12001 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
    12061 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
    12121 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
    12181 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca
    12241 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
    12301 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
    12361 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
    12421 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
    12481 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
    12541 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
    12601 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
    12661 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
    12721 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
    12781 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
    12841 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc
    12901 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa
    12961 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc
    13021 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt
    13081 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt
    13141 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac
    13201 tgaattaacg ccgaattaat tcctaggcca ccatgttggg cccggcgcgc caagcttggc
    13261 tgcaggtcga cctcgcatgc ctgcaggtca acatggtgga gcacgacacg cttgtctact
    13321 ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa
    13381 gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga
    13441 agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca
    13501 tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
    13561 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
    13621 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
    13681 aaggaagttc atttcatttg gagagga
//
