LOCUS       Exported                6293 bp DNA     circular SYN 27-NOV-2024
DEFINITION  Exported.
ACCESSION   V013006
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6293)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(693..797)
                     /label=AmpR promoter
     misc_feature    complement(1081..1929)
                     /label=RP10
     primer_bind     2126..2142
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2144..3481
                     /label=MET3 promoter
                     /note="Candida albicans MET3 promoter"
     misc_feature    3523..4877
                     /label=CaURA3
     CDS             3941..4750
                     /gene="URA3"
                     /label=Orotidine 5'-phosphate decarboxylase
                     /note="Orotidine 5'-phosphate decarboxylase from Candida 
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#: 
                     P13649"
     primer_bind     complement(4898..4914)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4922..4938)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4946..4976)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4991..5012)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5300..5888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6284..6293,1..692))
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIAEGG"
ORIGIN
        1 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
       61 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      121 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      181 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      241 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      301 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      361 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      421 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      481 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      541 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      601 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      661 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      721 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
      781 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
      841 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
      901 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
      961 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     1021 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     1081 cagatcctta tccttaaaca gattctaaaa caacatcttt gaaaccagaa gaaactttct
     1141 tacctttttc ttcggttgaa ccttcaccgt gcaaagccaa taatgaaccc aagtcgaatt
     1201 ttggttgttt caacaatttg acttttctga tgtggacatt ttgtaatggg aaaatggttt
     1261 gggtggattt ttcaatttca cggccaatga cttctggaat caatttggaa gttaattgag
     1321 ctaaagtaca gttggaaact tctctttgca taatttcaat catcttcttt ctgacttctc
     1381 tcaatttaga ggattgagcg tgagtagttt tcttgatttg gtttggttgt cttttggtga
     1441 aagcaatggc aaaaactctc aaaacgtaat cgtcggaagt tttaacagtg acattagctt
     1501 caactaatga ttgccatttt ctgaccaatg atcttaattt gtcagaagtg aaatccaaac
     1561 catgaggcct ccatgaggcc tccatgaggc ctccatgagg cctccatgga ggcctcatgg
     1621 aagttggtca acaagttttt accttggact tcatcaactc tcaatttgat ttttctgtaa
     1681 gagtggtctt cggaaccttg taagtcggcc aaacaaactt cgaaaactct acctttcaag
     1741 ccatcagcgg cattctttaa accggtagat ctgttgatca aagttttacc aacatttctg
     1801 ttttcaaaag tggttggagc tttgatgtca aaccaatctt ttctggtgaa tgggtcaacg
     1861 accttctttt ttaatccttt ctttcctttg gacaatctct tgtttttacc gacagccatg
     1921 ttgtacttgc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     1981 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     2041 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     2101 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgtc tattccaagc
     2161 ctgtggcttt ttttggtgat tcacaataca taaattggca aatacctctt ctatagactt
     2221 taacctttga tgaaatggta tggataaacc acttttgtgg tcagggtata tatattttta
     2281 ttccaatctg gcctttataa attcaaattt ccaaggggac tctgctaaac cttctgatat
     2341 aactataact ctaataatgg ctgtaaaaag tgtgaaaaaa agatgatatt cacaaaaaag
     2401 ataaagggga tttcatattg ggtatctaga tttaaacatc gtatttcaac aatgaaacta
     2461 gaatcaatac gaaatccagg gcacaactgt gttcattccc ttgggaatta tactgagtaa
     2521 tgggaagaat gtgcaaaaaa tacccgtgtc ttatataatt tccatttgtg tagggcttaa
     2581 aagtggacaa cttcaggtta cgaaaccacg aaaatgtcta aacgtttgat caaattcgta
     2641 actcatggaa aatcacagca acaaattaag gatggatata tccggtactt acaaataaaa
     2701 acactaaaga agtataaatt ttgaactgaa attctataga aaatcttttt tttttgggta
     2761 aaattatgcg attgtggctc atagtaacgt aatcatacag ttcaacaaac atcattttgt
     2821 caaaggattt ctaatcccat tctttttcta cttggctttt gagtatatat atatatatac
     2881 atcctttgtt catcatcatg ttcgattgaa tttttttctt ggttgttatt agattgtcta
     2941 cttgagtttt tcttggtgca aattgtggtg tttttctaac aaatttttca acatttaaag
     3001 tatttctgga acttttctac aaggaacgac atagatccat taatgcgccc ctctaaaaca
     3061 atacccataa tgtcttctta agtatgcatc tgcttataaa tgtttctgga tttactttag
     3121 ttagaatgtc gacacgacaa tgaactattg ctgtggatca cgtgcgatca ctgaattatg
     3181 agtttctttt tatttttctt tttgggcttt tttttgcaat tttttttttc cacctttgct
     3241 aattgcgtag tcacatataa tgtatacaat ttttgcaact ctgacactat aattgatcca
     3301 ataataaagt atataataga tcatcaagta tacgtaatct ccccaaaaaa aaaattgcat
     3361 aaaataaaat ctataaacct tttttagatt tttagttgtt ccttttttct ttctattctt
     3421 caacttccaa gtgttgtcac tttcttcctg ttaactatat ttaaaagtaa ataccctccc
     3481 cggatcctct agagtcgacc tgcaggcatg caagcttccg gataatagga attgatttgg
     3541 atggtataaa cggaaacaaa aaaaagagct ggtactactt tctttaaaat tattttatta
     3601 tttgatttta tttaatagta tatattatat tttgaacgta gattattttg ttgaaagttg
     3661 ctgtagtgcc attgattcgt aacactaatt ctgtattagt cattcctctt gtttgatagt
     3721 atccaaaaaa acggctattt ttttgcaatc ttatttcctg catattatac agataacata
     3781 atgaaagaaa aaatcttttt ttttgttctt caatgatgat ttcaaccatt cttttaaaca
     3841 ttgatcaatt cctgagcaac aaccccatac acactggttt atatatcgcc ccttttacag
     3901 ttgaagaaag aaatagaaat agaaatagca aacaaaagat atgacagtca acactaagac
     3961 ctatagtgag agagcagaaa ctcatgcctc accagtagca caacgattat ttcgattaat
     4021 ggaactgaag aaaaccaatt tatgtgcatc aattgatgtt gataccacta aggaattcct
     4081 tgaattaatt gataaattgg gtccttatgt atgcttaatc aagactcata ttgatataat
     4141 caatgatttt tcctatgaat ccactattga accattatta gaactttcac gtaaacatca
     4201 atttatgatt tttgaagata gaaaatttgc tgatattggt aataccgtga agaaacaata
     4261 tattggtgga gtttataaaa ttagtagttg ggcagatatt actaatgctc atggtgtcac
     4321 tgggaatgga gtagttgaag gattaaaaca gggagctaaa gaaaccacca ccaaccaaga
     4381 gccaagaggg ttattgatgt tagctgaatt atcatcagtg ggatcattag catatggaga
     4441 atattctcaa aaaactgttg aaattgctaa atccgataag gaatttgtta ttggatttat
     4501 tgcccaacgt gatatgggtg gacaagaaga aggatttgat tggcttatta tgacacctgg
     4561 agttggatta gatgataaag gtgatggatt aggacaacaa tatagaactg ttgatgaagt
     4621 tgttagcact ggaactgata ttatcattgt tggtagagga ttgtttggta aaggaagaga
     4681 tccagatatt gaaggtaaaa ggtatagaga tgctggttgg aatgcttatt tgaaaaaaac
     4741 tggccaatta taaatgtgaa gggggagatt ttcactttat tagatttgta tatatgtata
     4801 ataaataaat aaataagtta aataaataat taaataaggg tggtaattat tactatttac
     4861 aatcaaaggt ggtccttcta agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     4921 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4981 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5041 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     5101 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5161 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5221 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5281 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5341 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5401 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5461 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5521 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5581 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5641 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5701 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5761 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5821 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5881 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5941 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6001 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6061 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     6121 ttgcctgact ccccgtcgtg tagataacta cgatacgggg agggcttacc atctggcccc
     6181 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6241 cagccagccg gaaggccgaa gcggcagaag tggtcctgca actttatccg cct
//
