LOCUS       Exported               12694 bp DNA     circular SYN 28-NOV-2025
DEFINITION  Integration-free (episomal) expression of human SOX2 and KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12694)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 Apr 3. ():.
  PUBMED    21460823
REFERENCE   2  (bases 1 to 12694)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 12694)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12694)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2011 Apr 3. ():."
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..662
                     /label=chicken beta-actin promoter
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids,
                     forward primer"
     CDS             1744..2694
                     /codon_start=1
                     /label=hSOX2
                     /note="Homo sapiens transcription factor SOX-2 gene.
                     Belongs to the SRY-related HMG-box (SOX) family of 
                     transcription factors, which is involved in the regulation 
                     of embryonic development and in the determination of cell 
                     fate"
                     /translation="MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPM
                     NAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKE
                     HPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHM
                     NGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPT
                     YSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGA
                     EVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM"
     CDS             2719..2784
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             2809..4218
                     /codon_start=1
                     /label=hKLF4
                     /note="Homo sapiens Kruppel-like factor 4 (Klf4) gene.
                     Belongs to the relatively large family of SP1-like 
                     transcription factors and is involved in the regulation of 
                     proliferation, differentiation, apoptosis and somatic cell 
                     reprogramming."
                     /translation="MAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSHMKRL
                     PPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSL
                     THPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLL
                     YGRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVL
                     KASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGA
                     GPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYP
                     SFLPDQMQPQVPPLHYQELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYT
                     KSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSR
                     SDHLALHMKRHF"
     misc_feature    4272..4860
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(4951..4970)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5016..5071
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5070..5089)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also
                     called rb-glob-pA-term-R"
     primer_bind     complement(5432..5448)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5432..5448)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5445..5467)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5456..5472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5480..5510)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5525..5546)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     protein_bind    5635..5668
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6129..8051
                     /codon_start=1
                     /label=EBNA1
                     /note="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    complement(8222..8255)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      8442..10231
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(10772..10789)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10943..11531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11705..12562)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12563..12667)
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgcccttc
     1741 accatgtaca acatgatgga gacggagctg aagccgccgg gcccgcagca aacttcgggg
     1801 ggcggcggcg gcaactccac cgcggcggcg gccggcggca accagaaaaa cagcccggac
     1861 cgcgtcaagc ggcccatgaa tgccttcatg gtgtggtccc gcgggcagcg gcgcaagatg
     1921 gcccaggaga accccaagat gcacaactcg gagatcagca agcgcctggg cgccgagtgg
     1981 aaacttttgt cggagacgga gaagcggccg ttcatcgacg aggctaagcg gctgcgagcg
     2041 ctgcacatga aggagcaccc ggattataaa taccggcccc ggcggaaaac caagacgctc
     2101 atgaagaagg ataagtacac gctgcccggc gggctgctgg cccccggcgg caatagcatg
     2161 gcgagcgggg tcggggtggg cgccggcctg ggcgcgggcg tgaaccagcg catggacagt
     2221 tacgcgcaca tgaacggctg gagcaacggc agctacagca tgatgcagga ccagctgggc
     2281 tacccgcagc acccgggcct caatgcgcac ggcgcagcgc agatgcagcc catgcaccgc
     2341 tacgacgtga gcgccctgca gtacaactcc atgaccagct cgcagaccta catgaacggc
     2401 tcgcccacct acagcatgtc ctactcgcag cagggcaccc ctggcatggc tcttggctcc
     2461 atgggttcgg tggtcaagtc cgaggccagc tccagccccc ctgtggttac ctcttcctcc
     2521 cactccaggg cgccctgcca ggccggggac ctccgggaca tgatcagcat gtatctcccc
     2581 ggcgccgagg tgccggaacc cgccgccccc agcagacttc acatgtccca gcactaccag
     2641 agcggcccgg tgcccggcac ggccattaac ggcacactgc ccctctcaca catgggggat
     2701 ctcaaaattg tcgctcctgt caaacaaact cttaactttg atttactcaa actggctggg
     2761 gatgtagaaa gcaatccagg tccactcatg aacaattcgc ccttcaccat ggctgtcagt
     2821 gacgcgctgc tcccatcttt ctccacgttc gcgtctggcc cggcgggaag ggagaagaca
     2881 ctgcgtcaag caggtgcccc gaataaccgc tggcgggagg agctctccca catgaagcga
     2941 cttcccccag tgcttcccgg ccgcccctat gacctggcgg cggcgaccgt ggccacagac
     3001 ctggagagcg gcggagccgg tgcggcttgc ggcggtagca acctggcgcc cctacctcgg
     3061 agagagaccg aggagttcaa cgatctcctg gacctggact ttattctctc caattcgctg
     3121 acccatcctc cggagtcagt ggccgccacc gtgtcctcgt cagcgtcagc ctcctcttcg
     3181 tcgtcgccgt cgagcagcgg ccctgccagc gcgccctcca cctgcagctt cacctatccg
     3241 atccgggccg ggaacgaccc gggcgtggcg ccgggcggca cgggcggagg cctcctctat
     3301 ggcagggagt ccgctccccc tccgacggct cccttcaacc tggcggacat caacgacgtg
     3361 agcccctcgg gcggcttcgt ggccgagctc ctgcggccag aattggaccc ggtgtacatt
     3421 ccgccgcagc agccgcagcc gccaggtggc gggctgatgg gcaagttcgt gctgaaggcg
     3481 tcgctgagcg cccctggcag cgagtacggc agcccgtcgg tcatcagcgt cagcaaaggc
     3541 agccctgacg gcagccaccc ggtggtggtg gcgccctaca acggcgggcc gccgcgcacg
     3601 tgccccaaga tcaagcagga ggcggtctct tcgtgcaccc acttgggcgc tggaccccct
     3661 ctcagcaatg gccaccggcc ggctgcacac gacttccccc tggggcggca gctccccagc
     3721 aggactaccc cgaccctggg tcttgaggaa gtgctgagca gcagggactg tcaccctgcc
     3781 ctgccgcttc ctcccggctt ccatccccac ccggggccca attacccatc cttcctgccc
     3841 gatcagatgc agccgcaagt cccgccgctc cattaccaag agctcatgcc acccggttcc
     3901 tgcatgccag aggagcccaa gccaaagagg ggaagacgat cgtggccccg gaaaaggacc
     3961 gccacccaca cttgtgatta cgcgggctgc ggcaaaacct acacaaagag ttcccatctc
     4021 aaggcacacc tgcgaaccca cacaggtgag aaaccttacc actgtgactg ggacggctgt
     4081 ggatggaaat tcgcccgctc agatgaactg accaggcact accgtaaaca cacggggcac
     4141 cgcccgttcc agtgccaaaa atgcgaccga gcattttcca ggtcggacca cctcgcctta
     4201 cacatgaaga gacattttta aaagggcgaa ttgcctgcag gaattcaagc ttcggggact
     4261 agtcatatga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4321 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4381 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4441 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4501 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4561 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4621 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     4681 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4741 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4801 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4861 atcggtacaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     4921 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     4981 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     5041 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     5101 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     5161 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     5221 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     5281 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     5341 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     5401 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5461 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5521 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5581 gaaacctgtc gtgccagcgg atctcaattc cgatcatatt caataaccct taatataact
     5641 tcgtataatg tatgctatac gaagttatta ggtctgaaga ggagtttacg tccagccaag
     5701 cttaggatca attctcatgt ttgacagctt atcatcgata agctgatcct cacaggccgc
     5761 acccagcttt tcttccgttg ccccagtagc atctctgtct ggtgaccttg aagaggaaga
     5821 ggaggggtcc cgagaatccc catccctacc gtccagcaaa aagggggacg aggaatttga
     5881 ggcctggctt gaggctcagg acgcaaatct tgaggatgtt cagcgggagt tttccgggct
     5941 gcgagtaatt ggtgatgagg acgaggatgg ttcggaggat ggggaatttt cagacctgga
     6001 tctgtctgac agcgaccatg aaggggatga gggtgggggg gctgttggag ggggcaggag
     6061 tctgcactcc ctgtattcac tgagcgtcgt ctaataaaga tgtctattga tctcttttag
     6121 tgtgaatcat gtctgacgag gggccaggta caggacctgg aaatggccta ggagagaagg
     6181 gagacacatc tggaccagaa ggctccggcg gcagtggacc tcaaagaaga gggggtgata
     6241 accatggacg aggacgggga agaggacgag gacgaggagg cggaagacca ggagccccgg
     6301 gcggctcagg atcagggcca agacatagag atggtgtccg gagaccccaa aaacgtccaa
     6361 gttgcattgg ctgcaaaggg acccacggtg gaacaggagc aggagcagga gcgggagggg
     6421 caggagcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg gcaggagggg
     6481 caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggagga ggggcaggag
     6541 gggcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca ggaggggcag
     6601 gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag
     6661 gaggaggggc aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag
     6721 caggaggagg ggcaggagca ggaggggcag gaggggcagg aggggcagga gcaggagggg
     6781 caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggaggg gcaggagcag
     6841 gaggggcagg agcaggaggg gcaggagcag gaggggcagg aggggcagga gcaggagggg
     6901 caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggaggg gcaggagggg
     6961 caggagcagg aggaggggca ggaggggcag gagcaggagg ggcaggaggg gcaggagcag
     7021 gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga ggaggggcag
     7081 gagcaggagg ggcaggagca ggaggtggag gccggggtcg aggaggcagt ggaggccggg
     7141 gtcgaggagg tagtggaggc cggggtcgag gaggtagtgg aggccgccgg ggtagaggac
     7201 gtgaaagagc caggggggga agtcgtgaaa gagccagggg gagaggtcgt ggacgtggag
     7261 aaaagaggcc caggagtccc agtagtcagt catcatcatc cgggtctcca ccgcgcaggc
     7321 cccctccagg tagaaggcca tttttccacc ctgtagggga agccgattat tttgaatacc
     7381 accaagaagg tggcccagat ggtgagcctg acgtgccccc gggagcgata gagcagggcc
     7441 ccgcagatga cccaggagaa ggcccaagca ctggaccccg gggtcagggt gatggaggca
     7501 ggcgcaaaaa aggagggtgg tttggaaagc atcgtggtca aggaggttcc aacccgaaat
     7561 ttgagaacat tgcagaaggt ttaagagctc tcctggctag gagtcacgta gaaaggacta
     7621 ccgacgaagg aacttgggtc gccggtgtgt tcgtatatgg aggtagtaag acctcccttt
     7681 acaacctaag gcgaggaact gcccttgcta ttccacaatg tcgtcttaca ccattgagtc
     7741 gtctcccctt tggaatggcc cctggacccg gcccacaacc tggcccgcta agggagtcca
     7801 ttgtctgtta tttcatggtc tttttacaaa ctcatatatt tgctgaggtt ttgaaggatg
     7861 cgattaagga ccttgttatg acaaagcccg ctcctacctg caatatcagg gtgactgtgt
     7921 gcagctttga cgatggagta gatttgcctc cctggtttcc acctatggtg gaaggggctg
     7981 ccgcggaggg tgatgacgga gatgacggag atgaaggagg tgatggagat gagggtgagg
     8041 aagggcagga gtgatgtaac ttgttaggag acgccctcaa tcgtattaaa agccgtgtat
     8101 tcccccgcac taaagaataa atccccagta gacatcatgc gtgctgttgg tgtatttctg
     8161 gccatctgtc ttgtcaccat tttcgtcctc ccaacatggg gcaattgccg gaacccttaa
     8221 tataacttcg tataatgtat gctatacgaa gttattaggt ccctcgaaga ggttcactag
     8281 cggatctcaa ttgggcatac ccatgttgtc acgtcactca gctccgcgct caacaccttc
     8341 tcgcgttgga aaacattagc gacatttacc tggtgagcaa tcagacatgc gacggcttta
     8401 gcctggcctc cttaaattca cctaagaatg ggagcaacca gcaggaaaag gacaagcagc
     8461 gaaaattcac gcccccttgg gaggtggcgg catatgcaaa ggatagcact cccactctac
     8521 tactgggtat catatgctga ctgtatatgc atgaggatag catatgctac ccggatacag
     8581 attaggatag catatactac ccagatatag attaggatag catatgctac ccagatatag
     8641 attaggatag cctatgctac ccagatataa attaggatag catatactac ccagatatag
     8701 attaggatag catatgctac ccagatatag attaggatag cctatgctac ccagatatag
     8761 attaggatag catatgctac ccagatatag attaggatag catatgctat ccagatattt
     8821 gggtagtata tgctacccag atataaatta ggatagcata tactacccta atctctatta
     8881 ggatagcata tgctacccgg atacagatta ggatagcata tactacccag atatagatta
     8941 ggatagcata tgctacccag atatagatta ggatagccta tgctacccag atataaatta
     9001 ggatagcata tactacccag atatagatta ggatagcata tgctacccag atatagatta
     9061 ggatagccta tgctacccag atatagatta ggatagcata tgctatccag atatttgggt
     9121 agtatatgct acccatggca acattagccc accgtgctct cagcgacctc gtgaatatga
     9181 ggaccaacaa ccctgtgctt ggcgctcagg cgcaagtgtg tgtaatttgt cctccagatc
     9241 gcagcaatcg cgcccctatc ttggcccgcc cacctactta tgcaggtatt ccccggggtg
     9301 ccattagtgg ttttgtgggc aagtggtttg accgcagtgg ttagcggggt tacaatcagc
     9361 caagttatta cacccttatt ttacagtcca aaaccgcagg gcggcgtgtg ggggctgacg
     9421 cgtgccccca ctccacaatt tcaaaaaaaa gagtggccac ttgtctttgt ttatgggccc
     9481 cattggcgtg gagccccgtt taattttcgg gggtgttaga gacaaccagt ggagtccgct
     9541 gctgtcggcg tccactctct ttccccttgt tacaaataga gtgtaacaac atggttcacc
     9601 tgtcttggtc cctgcctggg acacatctta ataaccccag tatcatattg cactaggatt
     9661 atgtgttgcc catagccata aattcgtgtg agatggacat ccagtcttta cggcttgtcc
     9721 ccaccccatg gatttctatt gttaaagata ttcagaatgt ttcattccta cactagtatt
     9781 tattgcccaa ggggtttgtg agggttatat tggtgtcata gcacaatgcc accactgaac
     9841 cccccgtcca aattttattc tgggggcgtc acctgaaacc ttgttttcga gcacctcaca
     9901 tacaccttac tgttcacaac tcagcagtta ttctattagc taaacgaagg agaatgaaga
     9961 agcaggcgaa gattcaggag agttcactgc ccgctccttg atcttcagcc actgcccttg
    10021 tgactaaaat ggttcactac cctcgtggaa tcctgacccc atgtaaataa aaccgtgaca
    10081 gctcatgggg tgggagatat cgctgttcct taggaccctt ttactaaccc taattcgata
    10141 gcatatgctt cccgttgggt aacatatgct attgaattag ggttagtctg gatagtatat
    10201 actactaccc gggaagcata tgctacccgt ttagggttaa caagggggcc ttataaacac
    10261 tattgctaat gccctcttga gggtccgctt atcggtagct acacaggccc ctctgattga
    10321 cgttggtgta gcctcccgta gtcttcctgg gcccctggga ggtacatgtc ccccagcatt
    10381 ggtgtaagag cttcagccaa gagttacaca taaaggcaat gttgtgttgc agtccacaga
    10441 ctgcaaagtc tgctccagga tgaaagccac tcagtgttgg caaatgtgca catccattta
    10501 taaggatgtc aactacagtc agagaacccc tttgtgtttg gtcccccccc gtgtcacatg
    10561 tggaacaggg cccagttggc aagttgtacc aaccaactga agggattaca tgcactgccc
    10621 cgcgaagaag gggcagagat gtcgtagtca ggtttagttc gtccggggcg gggatcgatc
    10681 ctctagagtc gacgctagcg gatccgctgc attaatgaat cggccaacgc gcggggagag
    10741 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    10801 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    10861 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    10921 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    10981 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    11041 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    11101 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    11161 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    11221 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    11281 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    11341 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    11401 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    11461 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    11521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    11581 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    11641 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    11701 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    11761 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    11821 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    11881 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    11941 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    12001 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    12061 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    12121 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    12181 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    12241 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    12301 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    12361 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    12421 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    12481 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    12541 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    12601 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    12661 ttccgcgcac atttccccga aaagtgccac ctgg
//
