LOCUS       Exported               11681 bp DNA     circular SYN 28-NOV-2025
DEFINITION  Integration-free (episomal) expression of human OCT3/4 and shRNA 
            against p53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11681)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 Apr 3. ():.
  PUBMED    21460823
REFERENCE   2  (bases 1 to 11681)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11681)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11681)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2011 Apr 3. ():."
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids,
                     forward primer"
     CDS             1743..2822
                     /codon_start=1
                     /label=hOct4
                     /note="Homo sapiens Oct-4 gene. Encodes a transcription
                     factor containing a POU homeodomain that plays a key role 
                     in embryonic development and stem cell pluripotency. 
                     Aberrant expression in adult tissues is associated with 
                     tumorigenesis."
                     /translation="MAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGP
                     GIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVGVE
                     SNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITLGYT
                     QADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQEICK
                     AETLVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRVWFCNR
                     RQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALYSSVPFP
                     EGEAFPPVSVTTLGSPMHSN"
     misc_feature    2866..3454
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(3545..3564)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3610..3665
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3664..3683)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also
                     called rb-glob-pA-term-R"
     primer_bind     complement(4026..4042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4026..4042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4039..4061)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4050..4066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4074..4104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4119..4140)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     protein_bind    4229..4262
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4723..6645
                     /codon_start=1
                     /label=EBNA1
                     /note="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    complement(6816..6849)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      7036..8825
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     promoter        9295..9608
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das
                     et al., 1988)"
     primer_bind     complement(9759..9776)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9930..10518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10689..11549)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11312..11331
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11550..11654)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcccttca
     1741 ccatggcggg acacctggct tcggatttcg ccttctcgcc ccctccaggt ggtggaggtg
     1801 atgggccagg ggggccggag ccgggctggg ttgatcctcg gacctggcta agcttccaag
     1861 gccctcctgg agggccagga atcgggccgg gggttgggcc aggctctgag gtgtggggga
     1921 ttcccccatg ccccccgccg tatgagttct gtggggggat ggcgtactgt gggccccagg
     1981 ttggagtggg gctagtgccc caaggcggct tggagacctc tcagcctgag ggcgaagcag
     2041 gagtcggggt ggagagcaac tccgatgggg cctccccgga gccctgcacc gtcacccctg
     2101 gtgccgtgaa gctggagaag gagaagctgg agcaaaaccc ggaggagtcc caggacatca
     2161 aagctctgca gaaagaactc gagcaatttg ccaagctcct gaagcagaag aggatcaccc
     2221 tgggatatac acaggccgat gtggggctca ccctgggggt tctatttggg aaggtattca
     2281 gccaaacgac catctgccgc tttgaggctc tgcagcttag cttcaagaac atgtgtaagc
     2341 tgcggccctt gctgcagaag tgggtggagg aagctgacaa caatgaaaat cttcaggaga
     2401 tatgcaaagc agaaaccctc gtgcaggccc gaaagagaaa gcgaaccagt atcgagaacc
     2461 gagtgagagg caacctggag aatttgttcc tgcagtgccc gaaacccaca ctgcagcaga
     2521 tcagccacat cgcccagcag cttgggctcg agaaggatgt ggtccgagtg tggttctgta
     2581 accggcgcca gaagggcaag cgatcaagca gcgactatgc acaacgagag gattttgagg
     2641 ctgctgggtc tcctttctca gggggaccag tgtcctttcc tctggcccca gggccccatt
     2701 ttggtacccc aggctatggg agccctcact tcactgcact gtactcctcg gtccctttcc
     2761 ctgaggggga agcctttccc cctgtctctg tcaccactct gggctctccc atgcattcaa
     2821 actgaggtaa gggcgaattc aagcttcggg gactagtcat atgataatca acctctggat
     2881 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2941 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3001 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3061 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3121 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3181 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3241 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     3301 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3361 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3421 acgagtcgga tctccctttg ggccgcctcc ccgcatcggt acaattcact cctcaggtgc
     3481 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact
     3541 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
     3601 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     3661 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
     3721 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa
     3781 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     3841 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     3901 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     3961 tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggcgtaa
     4021 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     4081 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     4141 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatctca
     4201 attccgatca tattcaataa cccttaatat aacttcgtat aatgtatgct atacgaagtt
     4261 attaggtctg aagaggagtt tacgtccagc caagcttagg atcaattctc atgtttgaca
     4321 gcttatcatc gataagctga tcctcacagg ccgcacccag cttttcttcc gttgccccag
     4381 tagcatctct gtctggtgac cttgaagagg aagaggaggg gtcccgagaa tccccatccc
     4441 taccgtccag caaaaagggg gacgaggaat ttgaggcctg gcttgaggct caggacgcaa
     4501 atcttgagga tgttcagcgg gagttttccg ggctgcgagt aattggtgat gaggacgagg
     4561 atggttcgga ggatggggaa ttttcagacc tggatctgtc tgacagcgac catgaagggg
     4621 atgagggtgg gggggctgtt ggagggggca ggagtctgca ctccctgtat tcactgagcg
     4681 tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca
     4741 ggtacaggac ctggaaatgg cctaggagag aagggagaca catctggacc agaaggctcc
     4801 ggcggcagtg gacctcaaag aagagggggt gataaccatg gacgaggacg gggaagagga
     4861 cgaggacgag gaggcggaag accaggagcc ccgggcggct caggatcagg gccaagacat
     4921 agagatggtg tccggagacc ccaaaaacgt ccaagttgca ttggctgcaa agggacccac
     4981 ggtggaacag gagcaggagc aggagcggga ggggcaggag caggaggggc aggagcagga
     5041 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagg ggcaggagca
     5101 ggaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggagga
     5161 ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca
     5221 ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggagca
     5281 ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggaggg
     5341 gcaggagggg caggaggggc aggagcagga ggggcaggag caggaggagg ggcaggaggg
     5401 gcaggagggg caggagcagg aggggcagga gcaggagggg caggagcagg aggggcagga
     5461 gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca
     5521 ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg
     5581 gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
     5641 ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggggcagg agcaggaggt
     5701 ggaggccggg gtcgaggagg cagtggaggc cggggtcgag gaggtagtgg aggccggggt
     5761 cgaggaggta gtggaggccg ccggggtaga ggacgtgaaa gagccagggg gggaagtcgt
     5821 gaaagagcca gggggagagg tcgtggacgt ggagaaaaga ggcccaggag tcccagtagt
     5881 cagtcatcat catccgggtc tccaccgcgc aggccccctc caggtagaag gccatttttc
     5941 caccctgtag gggaagccga ttattttgaa taccaccaag aaggtggccc agatggtgag
     6001 cctgacgtgc ccccgggagc gatagagcag ggccccgcag atgacccagg agaaggccca
     6061 agcactggac cccggggtca gggtgatgga ggcaggcgca aaaaaggagg gtggtttgga
     6121 aagcatcgtg gtcaaggagg ttccaacccg aaatttgaga acattgcaga aggtttaaga
     6181 gctctcctgg ctaggagtca cgtagaaagg actaccgacg aaggaacttg ggtcgccggt
     6241 gtgttcgtat atggaggtag taagacctcc ctttacaacc taaggcgagg aactgccctt
     6301 gctattccac aatgtcgtct tacaccattg agtcgtctcc cctttggaat ggcccctgga
     6361 cccggcccac aacctggccc gctaagggag tccattgtct gttatttcat ggtcttttta
     6421 caaactcata tatttgctga ggttttgaag gatgcgatta aggaccttgt tatgacaaag
     6481 cccgctccta cctgcaatat cagggtgact gtgtgcagct ttgacgatgg agtagatttg
     6541 cctccctggt ttccacctat ggtggaaggg gctgccgcgg agggtgatga cggagatgac
     6601 ggagatgaag gaggtgatgg agatgagggt gaggaagggc aggagtgatg taacttgtta
     6661 ggagacgccc tcaatcgtat taaaagccgt gtattccccc gcactaaaga ataaatcccc
     6721 agtagacatc atgcgtgctg ttggtgtatt tctggccatc tgtcttgtca ccattttcgt
     6781 cctcccaaca tggggcaatt gccggaaccc ttaatataac ttcgtataat gtatgctata
     6841 cgaagttatt aggtccctcg aagaggttca ctagcggatc tcaattgggc atacccatgt
     6901 tgtcacgtca ctcagctccg cgctcaacac cttctcgcgt tggaaaacat tagcgacatt
     6961 tacctggtga gcaatcagac atgcgacggc tttagcctgg cctccttaaa ttcacctaag
     7021 aatgggagca accagcagga aaaggacaag cagcgaaaat tcacgccccc ttgggaggtg
     7081 gcggcatatg caaaggatag cactcccact ctactactgg gtatcatatg ctgactgtat
     7141 atgcatgagg atagcatatg ctacccggat acagattagg atagcatata ctacccagat
     7201 atagattagg atagcatatg ctacccagat atagattagg atagcctatg ctacccagat
     7261 ataaattagg atagcatata ctacccagat atagattagg atagcatatg ctacccagat
     7321 atagattagg atagcctatg ctacccagat atagattagg atagcatatg ctacccagat
     7381 atagattagg atagcatatg ctatccagat atttgggtag tatatgctac ccagatataa
     7441 attaggatag catatactac cctaatctct attaggatag catatgctac ccggatacag
     7501 attaggatag catatactac ccagatatag attaggatag catatgctac ccagatatag
     7561 attaggatag cctatgctac ccagatataa attaggatag catatactac ccagatatag
     7621 attaggatag catatgctac ccagatatag attaggatag cctatgctac ccagatatag
     7681 attaggatag catatgctat ccagatattt gggtagtata tgctacccat ggcaacatta
     7741 gcccaccgtg ctctcagcga cctcgtgaat atgaggacca acaaccctgt gcttggcgct
     7801 caggcgcaag tgtgtgtaat ttgtcctcca gatcgcagca atcgcgcccc tatcttggcc
     7861 cgcccaccta cttatgcagg tattccccgg ggtgccatta gtggttttgt gggcaagtgg
     7921 tttgaccgca gtggttagcg gggttacaat cagccaagtt attacaccct tattttacag
     7981 tccaaaaccg cagggcggcg tgtgggggct gacgcgtgcc cccactccac aatttcaaaa
     8041 aaaagagtgg ccacttgtct ttgtttatgg gccccattgg cgtggagccc cgtttaattt
     8101 tcgggggtgt tagagacaac cagtggagtc cgctgctgtc ggcgtccact ctctttcccc
     8161 ttgttacaaa tagagtgtaa caacatggtt cacctgtctt ggtccctgcc tgggacacat
     8221 cttaataacc ccagtatcat attgcactag gattatgtgt tgcccatagc cataaattcg
     8281 tgtgagatgg acatccagtc tttacggctt gtccccaccc catggatttc tattgttaaa
     8341 gatattcaga atgtttcatt cctacactag tatttattgc ccaaggggtt tgtgagggtt
     8401 atattggtgt catagcacaa tgccaccact gaaccccccg tccaaatttt attctggggg
     8461 cgtcacctga aaccttgttt tcgagcacct cacatacacc ttactgttca caactcagca
     8521 gttattctat tagctaaacg aaggagaatg aagaagcagg cgaagattca ggagagttca
     8581 ctgcccgctc cttgatcttc agccactgcc cttgtgacta aaatggttca ctaccctcgt
     8641 ggaatcctga ccccatgtaa ataaaaccgt gacagctcat ggggtgggag atatcgctgt
     8701 tccttaggac ccttttacta accctaattc gatagcatat gcttcccgtt gggtaacata
     8761 tgctattgaa ttagggttag tctggatagt atatactact acccgggaag catatgctac
     8821 ccgtttaggg ttaacaaggg ggccttataa acactattgc taatgccctc ttgagggtcc
     8881 gcttatcggt agctacacag gcccctctga ttgacgttgg tgtagcctcc cgtagtcttc
     8941 ctgggcccct gggaggtaca tgtcccccag cattggtgta agagcttcag ccaagagtta
     9001 cacataaagg caatgttgtg ttgcagtcca cagactgcaa agtctgctcc aggatgaaag
     9061 ccactcagtg ttggcaaatg tgcacatcca tttataagga tgtcaactac agtcagagaa
     9121 cccctttgtg tttggtcccc ccccgtgtca catgtggaac agggcccagt tggcaagttg
     9181 taccaaccaa ctgaagggat tacatgcact gccccgcgaa gaaggggcag agatgtcgta
     9241 gtcaggttta gttcgtccgg ggcggggatc gatcctctag agtcgacgct agcggatccg
     9301 acgccgccat ctctaggccc gcgccggccc cctcgcacag acttgtggga gaagctcggc
     9361 tactcccctg ccccggttaa tttgcatata atatttccta gtaactatag aggcttaatg
     9421 tgcgataaaa gacagataat ctgttctttt taatactagc tacattttac atgataggct
     9481 tggatttcta taagagatac aaatactaaa ttattatttt aaaaaacagc acaaaaggaa
     9541 actcacccta actgtaaagt aattgtgtgt tttgagacta taaatatccc ttggagaaaa
     9601 gccttgtttg ggccgactcc agtggtaatc tacttcaaga gagtagatta ccactggagt
     9661 cttttttgga attcctgcag cccgggggat ccgctgcatt aatgaatcgg ccaacgcgcg
     9721 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9781 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9841 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9901 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    10021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    10081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    10141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    10201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    10261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    10321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    10381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10501 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10561 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10621 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10681 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10741 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10801 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10861 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10921 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10981 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    11041 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    11101 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    11161 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    11221 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    11281 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    11341 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    11401 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11461 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11521 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11581 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11641 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg g
//
