LOCUS       40924_13990       11735 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCXGUS-P, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang GL.
  TITLE     A versatile zero background T-vector system for gene cloning and 
            functional genomics
  JOURNAL   Plant Physiol. 150 (3), 1111-1121 (2009)
  PUBMED    19403729
REFERENCE   2  (bases 1 to 11735)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang G-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2009) Department of Plant Pathology, The Ohio 
            State University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 
            43210, USA
REFERENCE   3  (bases 1 to 11735)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11735)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2009"; volume: "150"; issue: "3"; pages: 
            "1111-1121"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-APR-2009) Department of Plant Pathology, The Ohio State 
            University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(9..261)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(281..2089)
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             2440..2742
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2840..2856)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3059..3083
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4382..5008
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5445..6509
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6578..6772
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7116..7256
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7442..8030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8120..8911)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9336..9360
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9438..9612)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9655..10677)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10744..11421)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11612..11633
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11648..11678
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11686..11702
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11710..11726
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
       61 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
      121 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
      181 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
      241 ttgccaaatg tttgaacgat cggggaaatt cgagctctca ttgtttgcct ccctgctgcg
      301 gtttttcacc gaagttcatg ccagtccagc gtttttgcag cagaaaagcc gccgacttcg
      361 gtttgcggtc gcgagtgaag atccctttct tgttaccgcc aacgcgcaat atgccttgcg
      421 aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac gacggcgctg acgcgatcaa
      481 agacgcggtg atacatatcc agccatgcac actgatactc ttcactccac atgtcggtgt
      541 acattgagtg cagcccggct aacgtatcca cgccgtattc ggtgatgata atcggctgat
      601 gcagtttctc ctgccaggcc agaagttctt tttccagtac cttctctgcc gtttccaaat
      661 cgccgctttg gacataccat ccgtaataac ggttcaggca cagcacatca aagagatcgc
      721 tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac gcaggtgatc ggacgcgtcg
      781 ggtcgagttt acgcgttgct tccgccagtg gcgcgaaata ttcccgtgca ccttgcggac
      841 gggtatccgg ttcgttggca atactccaca tcaccacgct tgggtggttt ttgtcacgcg
      901 ctatcagctc tttaatcgcc tgtaagtgcg cttgctgagt ttccccgttg actgcctctt
      961 cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc aatgcctaaa gagaggttaa
     1021 agccgacagc agcagtttca tcaatcacca cgatgccatg ttcatctgcc cagtcgagca
     1081 tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt ggccccaatc cagtccatta
     1141 atgcgtggtc gtgcaccatc agcacgttat cgaatccttt gccacgcaag tccgcatctt
     1201 catgacgacc aaagccagta aagtagaacg gtttgtggtt aatcaggaac tgttcgccct
     1261 tcactgccac tgaccggatg ccgacgcgaa gcgggtagat atcacactct gtctggcttt
     1321 tggctgtgac gcacagttca tagagataac cttcacccgg ttgccagagg tgcggattca
     1381 ccacttgcaa agtcccgcta gtgccttgtc cagttgcaac cacctgttga tccgcatcac
     1441 gcagttcaac gctgacatca ccattggcca ccacctgcca gtcaacagac gcgtggttac
     1501 agtcttgcgc gacatgcgtc accacggtga tatcgtccac ccaggtgttc ggcgtggtgt
     1561 agagcattac gctgcgatgg attccggcat agttaaagaa atcatggaag taagactgct
     1621 ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg gatagtctgc cagttcagtt
     1681 cgttgttcac acaaacggtg atacgtacac ttttcccggc aataacatac ggcgtgacat
     1741 cggcttcaaa tggcgtatag ccgccctgat gctccatcac ttcctgatta ttgacccaca
     1801 ctttgccgta atgagtgacc gcatcgaaac gcagcacgat acgctggcct gcccaacctt
     1861 tcggtataaa gacttcgcgc tgataccaga cgttgcccgc ataattacga atatctgcat
     1921 cggcgaactg atcgttaaaa ctgcctggca cagcaattgc ccggctttct tgtaacgcgc
     1981 tttcccacca acgctgatca attccacagt tttcgcgatc cagactgaat gcccacaggc
     2041 cgtcgagttt tttgatttca cgggttgggg tttctacagg acgtaacatg gatccccaat
     2101 acttgtatgg ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
     2161 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg
     2221 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
     2281 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
     2341 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
     2401 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc
     2461 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
     2521 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
     2581 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     2641 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     2701 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt
     2761 atacacagcc agtctgcagg tcgacccata caagtattgg ggatcctcta gagtcgacct
     2821 gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     2881 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     2941 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga
     3001 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg
     3061 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt
     3121 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     3181 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
     3241 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
     3301 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
     3361 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
     3421 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
     3481 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
     3541 ccgcccggag ctggccagga tgcttgacca cctagccctg gcgacgttgt gacagtgacc
     3601 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
     3661 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
     3721 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
     3781 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
     3841 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
     3901 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
     3961 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
     4021 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     4081 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     4141 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
     4201 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
     4261 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     4321 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     4381 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     4441 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     4501 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     4561 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     4621 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     4681 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gcaaccgccg acctggtgga
     4741 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     4801 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     4861 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     4921 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     4981 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     5041 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     5101 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     5161 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     5221 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     5281 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     5341 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
     5401 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg
     5461 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     5521 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     5581 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     5641 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     5701 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     5761 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     5821 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     5881 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     5941 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     6001 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     6061 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     6121 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     6181 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     6241 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     6301 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     6361 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     6421 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     6481 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     6541 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
     6601 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     6661 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     6721 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     6781 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     6841 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
     6901 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     6961 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7021 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7081 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     7141 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     7201 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     7261 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7321 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7381 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7441 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7501 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7561 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7621 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7681 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7741 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7801 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7861 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7921 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7981 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8041 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8101 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
     8161 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
     8221 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
     8281 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
     8341 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
     8401 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
     8461 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
     8521 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
     8581 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
     8641 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
     8701 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gttgtccctt
     8761 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
     8821 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
     8881 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
     8941 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
     9001 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
     9061 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
     9121 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
     9181 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
     9241 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
     9301 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
     9361 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac
     9421 gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa
     9481 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
     9541 acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt
     9601 attatggaga aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt
     9661 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     9721 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     9781 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     9841 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg
     9901 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     9961 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
    10021 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
    10081 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
    10141 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
    10201 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
    10261 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
    10321 catagcctcc gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa
    10381 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat
    10441 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
    10501 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
    10561 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
    10621 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
    10681 tcattgcccc ccggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg
    10741 atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag gtcttgcgaa
    10801 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
    10861 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
    10921 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
    10981 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
    11041 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
    11101 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
    11161 accatgttat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
    11221 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
    11281 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
    11341 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
    11401 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
    11461 ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca
    11521 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    11581 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    11641 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    11701 aatttcacac aggaaacagc tatgaccatg attac
//
