LOCUS       40924_13985       10640 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCXGFP-P, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang GL.
  TITLE     A versatile zero background T-vector system for gene cloning and 
            functional genomics
  JOURNAL   Plant Physiol. 150 (3), 1111-1121 (2009)
  PUBMED    19403729
REFERENCE   2  (bases 1 to 10640)
  AUTHORS   Chen S, Songkumarn P, Liu J, Wang G-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2009) Department of Plant Pathology, The Ohio 
            State University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 
            43210, USA
REFERENCE   3  (bases 1 to 10640)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10640)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2009"; volume: "150"; issue: "3"; pages: 
            "1111-1121"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-APR-2009) Department of Plant Pathology, The Ohio State 
            University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(9..261)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(281..994)
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTMGYGVLCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1345..1647
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1745..1761)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1964..1988
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3287..3913
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4350..5414
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5483..5677
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6021..6161
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6347..6935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7025..7816)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8241..8265
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8343..8517)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8560..9582)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9649..10326)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10517..10538
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10553..10583
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10591..10607
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10615..10631
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
       61 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
      121 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
      181 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
      241 ttgccaaatg tttgaacgat cggggaaatt cgagctctca tttgtatagt tcatccatgc
      301 catgtgtaat cccagcagct gttacaaact caagaaggac catgtggtct ctcttttcgt
      361 tgggatcttt cgaaagggca gattgtgtgg acaggtaatg gttgtctggt aaaaggacag
      421 ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg cttccatctt
      481 caatgttgtg tctaattttg aagttaactt tgattccatt cttttgtttg tctgccatga
      541 tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg tccaagaatg tttccatctt
      601 ctttaaaatc aatacctttt aactcgattc tattaacaag ggtatcacct tcaaacttga
      661 cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat
      721 aaccttcggg catggcactc ttgaaaaagt catgccgttt catatgatct gggtatcttg
      781 aaaagcatag aacaccataa cccatagtag tgacaagtgt tggccatgga acaggtagtt
      841 ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca ccttcaccct
      901 ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca agaattggga
      961 caactccagt gaaaagttct tctcctttac ccatggatcc ccaatacttg tatggggctt
     1021 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     1081 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     1141 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     1201 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     1261 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     1321 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     1381 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     1441 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     1501 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     1561 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     1621 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     1681 gcaggtcgac ccatacaagt attggggatc ctctagagtc gacctgcagg catgcaagct
     1741 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     1801 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     1861 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
     1921 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
     1981 gggtaaacct aagagaaaag agcgtttatt agaataacgg atatttaaaa gggcgtgaaa
     2041 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
     2101 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
     2161 gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
     2221 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
     2281 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
     2341 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
     2401 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
     2461 caggatgctt gaccacctag ccctggcgac gttgtgacag tgaccaggct agaccgcctg
     2521 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
     2581 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
     2641 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
     2701 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
     2761 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
     2821 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
     2881 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
     2941 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
     3001 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
     3061 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     3121 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     3181 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     3241 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     3301 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     3361 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     3421 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     3481 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     3541 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     3601 gcagccaagc ccttacgaca tatgggcaac cgccgacctg gtggagctgg ttaagcagcg
     3661 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     3721 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     3781 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     3841 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     3901 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     3961 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     4021 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     4081 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     4141 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     4201 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     4261 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     4321 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     4381 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     4441 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     4501 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     4561 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     4621 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     4681 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     4741 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     4801 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     4861 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     4921 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     4981 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     5041 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     5101 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     5161 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     5221 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     5281 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     5341 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     5401 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     5461 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     5521 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     5581 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     5641 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     5701 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     5761 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     5821 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     5881 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     5941 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     6001 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     6061 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     6121 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     6181 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6241 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6301 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6541 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6781 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
     7021 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
     7081 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
     7141 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
     7201 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
     7261 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
     7321 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
     7381 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
     7441 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
     7501 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
     7561 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
     7621 atagcatcat gtccttttcc cgttccacat cataggttgt ccctttatac cggctgtccg
     7681 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
     7741 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
     7801 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
     7861 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
     7921 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
     7981 ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca
     8041 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
     8101 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
     8161 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
     8221 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
     8281 caacttaata acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg
     8341 gggatctgga ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag
     8401 tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac
     8461 cctataggaa ccctaattcc cttatctggg aactactcac acattattat ggagaaactc
     8521 gagcttgtcg atcgacagat ccggtcggca tctactctat ttctttgccc tcggacgagt
     8581 gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc
     8641 cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc
     8701 gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata
     8761 gagttggtca agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc
     8821 cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca
     8881 gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat
     8941 gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac
     9001 gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc
     9061 gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc
     9121 aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg
     9181 gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac
     9241 cggttgtaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc
     9301 acggcgggag atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg
     9361 aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc
     9421 ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg
     9481 agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag
     9541 aaacttctcg acagacgtcg cggtgagttc aggctttttc atatctcatt gccccccgga
     9601 tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct
     9661 ctccaaatga aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt
     9721 gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt
     9781 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     9841 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
     9901 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
     9961 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
    10021 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat
    10081 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
    10141 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
    10201 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
    10261 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
    10321 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
    10381 gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg
    10441 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    10501 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    10561 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10621 acagctatga ccatgattac
//
