LOCUS       pCXB-EBNA1        6410 bp DNA     circular SYN 20-JUL-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 6410)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6410)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6410)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(83..102)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    148..203
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(202..221)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also
                     called rb-glob-pA-term-R"
     primer_bind     complement(564..580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(588..604)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(612..642)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(657..678)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(803..820)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(974..1562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1736..2593)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2594..2698)
                     /label=AmpR promoter
     enhancer        2729..3108
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3110..3385
                     /label=chicken beta-actin promoter
     intron          3386..4402
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4410..4429
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids,
                     forward primer"
     CDS             4472..6394
                     /codon_start=1
                     /label=EBNA1
                     /note="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
ORIGIN
        1 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
       61 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
      121 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
      181 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
      241 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
      301 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
      361 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
      421 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
      481 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
      541 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      601 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      721 tcgtgccagc ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      781 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1381 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1621 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1681 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1741 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1801 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1861 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1921 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1981 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2041 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2101 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2161 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2221 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2281 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2341 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2401 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2461 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2521 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2581 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2641 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2701 catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta ttaatagtaa
     2761 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     2821 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2881 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     2941 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     3001 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     3061 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
     3121 cccacgttct gcttcactct ccccatctcc ccccctcccc acccccaatt ttgtatttat
     3181 ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggc gcgcgccagg
     3241 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa
     3301 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
     3361 taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg
     3421 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     3481 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     3541 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     3601 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     3661 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     3721 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     3781 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc
     3841 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     3901 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     3961 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg
     4021 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     4081 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     4141 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     4201 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     4261 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     4321 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     4381 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     4441 ttttggcaaa gaattcgccc ttgtcgacac catgtctgac gaggggccag gtacaggacc
     4501 tggaaatggc ctaggagaga agggagacac atctggacca gaaggctccg gcggcagtgg
     4561 acctcaaaga agagggggtg ataaccatgg acgaggacgg ggaagaggac gaggacgagg
     4621 aggcggaaga ccaggagccc cgggcggctc aggatcaggg ccaagacata gagatggtgt
     4681 ccggagaccc caaaaacgtc caagttgcat tggctgcaaa gggacccacg gtggaacagg
     4741 agcaggagca ggagcgggag gggcaggagc aggaggggca ggagcaggag gaggggcagg
     4801 agcaggagga ggggcaggag gggcaggagg ggcaggaggg gcaggagcag gaggaggggc
     4861 aggagcagga ggaggggcag gaggggcagg aggggcagga gcaggaggag gggcaggagc
     4921 aggaggaggg gcaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg
     4981 agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggaggggc
     5041 aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggagggg caggaggggc
     5101 aggaggggca ggagcaggag gggcaggagc aggaggaggg gcaggagggg caggaggggc
     5161 aggagcagga ggggcaggag caggaggggc aggagcagga ggggcaggag caggaggggc
     5221 aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag gaggggcagg
     5281 agcaggagga ggggcaggag gggcaggagc aggaggaggg gcaggagggg caggagcagg
     5341 aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag gggcaggagg
     5401 ggcaggagca ggaggagggg caggagcagg aggggcagga gcaggaggtg gaggccgggg
     5461 tcgaggaggc agtggaggcc ggggtcgagg aggtagtgga ggccggggtc gaggaggtag
     5521 tggaggccgc cggggtagag gacgtgaaag agccaggggg ggaagtcgtg aaagagccag
     5581 ggggagaggt cgtggacgtg gagaaaagag gcccaggagt cccagtagtc agtcatcatc
     5641 atccgggtct ccaccgcgca ggccccctcc aggtagaagg ccatttttcc accctgtagg
     5701 ggaagccgat tattttgaat accaccaaga aggtggccca gatggtgagc ctgacgtgcc
     5761 cccgggagcg atagagcagg gccccgcaga tgacccagga gaaggcccaa gcactggacc
     5821 ccggggtcag ggtgatggag gcaggcgcaa aaaaggaggg tggtttggaa agcatcgtgg
     5881 tcaaggaggt tccaacccga aatttgagaa cattgcagaa ggtttaagag ctctcctggc
     5941 taggagtcac gtagaaagga ctaccgacga aggaacttgg gtcgccggtg tgttcgtata
     6001 tggaggtagt aagacctccc tttacaacct aaggcgagga actgcccttg ctattccaca
     6061 atgtcgtctt acaccattga gtcgtctccc ctttggaatg gcccctggac ccggcccaca
     6121 acctggcccg ctaagggagt ccattgtctg ttatttcatg gtctttttac aaactcatat
     6181 atttgctgag gttttgaagg atgcgattaa ggaccttgtt atgacaaagc ccgctcctac
     6241 ctgcaatatc agggtgactg tgtgcagctt tgacgatgga gtagatttgc ctccctggtt
     6301 tccacctatg gtggaagggg ctgccgcgga gggtgatgac ggagatgacg gagatgaagg
     6361 aggtgatgga gatgagggtg aggaagggca ggagtgagga tccaagggcg
//
