LOCUS       Exported                5438 bp DNA     circular SYN 10-SEP-2025
DEFINITION  Exported.
ACCESSION   V001682
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5438)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5438)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(346..5438,1..345)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          989..994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1471..1476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..52
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        67..97
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    105..121
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        176..204
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    212..228
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    271..299
                     /label=tac promoter
                     /note="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    307..323
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             366..752
                     /gene="cheY"
                     /label=Chemotaxis protein CheY
                     /note="Chemotaxis protein CheY from Escherichia coli
                     (strain K12). Accession#: P0AE67"
     primer_bind     complement(810..826)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1013..1468
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1501..1605
                     /label=AmpR promoter
     CDS             1606..2463
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2637..3225
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3411..3552)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3961..4134)
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
     protein_bind    complement(4154..4170)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4178..4208)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4223..4244)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(4260..5339)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(5340..5417)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc attaggcacc
       61 ccaggcttta cactttatgc ttccggctcg tataatgtgt ggaattgtga gcggataaca
      121 atttcacaca ggaaacagga tcgatccatc gatgagctta ctccccatcc ccctgttgac
      181 aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt tcacacagga
      241 aacaggatca gcttactccc catccccctg ttgacaatta atcatcggct cgtataatgt
      301 gtggaattgt gagcggataa caatttcaca caggaaacag gatccatcga tgcttaggag
      361 gtcatatggc ggataaagaa cttaaatttt tggttgtgga tgacttttcc accatgcgac
      421 gcatagtgcg taacctgctg aaagagctgg gattcaataa tgttgaggaa gcggaagatg
      481 gcgtcgacgc tctcaataag ttgcaggcag gcggttatgg atttgttatc tccgactgga
      541 acatgcccaa tatggatggc ctggaattgc tgaaaacaat tcgtgcggat ggcgcgatgt
      601 cggcattgcc agtgttaatg gtgactgcag aagcgaagaa agagaacatc attgctgcgg
      661 cgcaagcggg ggccagtggc tatgtggtga agccatttac cgccgcgacg ctggaggaaa
      721 aactcaacaa aatctttgag aaactgggca tgtgaggatg cgactatgat gcaaccatca
      781 atcaaacctg ctgacgagca ttcagggggg atccactagt tctagagtcg acctgcagcc
      841 caagcttatc gatgataagc tgtcaaacat gagcagatct gagcccgcct aatgagcggg
      901 cttttttttc agatctgctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
      961 taatgtcatg ataataatgg tttcttagac gatgcgtcaa agcaaccata gtacgcgccc
     1021 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     1081 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     1141 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagagcttta
     1201 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     1261 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     1321 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
     1381 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     1441 tttaacaaaa tattaacgtt tacaatttca tcgtcaggtg gcacttttcg gggaaatgtg
     1501 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1561 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     1621 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1681 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1741 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1801 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     1861 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1921 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1981 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2041 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2101 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     2161 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2221 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2281 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2341 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2401 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2461 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2521 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2581 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2641 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2701 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2761 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     2821 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2881 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2941 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3001 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3061 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3121 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3181 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3241 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3301 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3361 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     3421 tattttctcc ttacgcatct gtgcggtatt tcacaccgca taatggtgca ctctcagtac
     3481 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     3541 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3601 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     3661 ttttcaccgt catcaccgaa acgcgcgagg cagaacgcca tcaaaaataa ttcgcgtctg
     3721 gccttcctgt agccagcttt catcaacatt aaatgtgagc gagtaacaac ccgtcggatt
     3781 ctccgtggga acaaacggcg gattgaccgt aatgggatag gtcacgttgg tgtagatggg
     3841 cgcatcgtaa ccgtgcatct gccagtttga ggggacgacg acagtatcgg cctcaggaag
     3901 atcgcactcc agccagcttt ccggcaccgc ttctggtgcc ggaaaccagg caaagcgcca
     3961 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     4021 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     4081 ttcccagtca cgacgttgta aaacgacggc cagtgaatcc gtaatcatgg tcatagctgt
     4141 ttcctgtgtg aaattgttat ccgctcacaa ttccacacat tatacgagcc ggaagcataa
     4201 agtgtaaagc ctggggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac
     4261 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4321 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
     4381 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     4441 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     4501 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     4561 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     4621 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     4681 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     4741 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     4801 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     4861 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     4921 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     4981 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     5041 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     5101 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     5161 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     5221 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     5281 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     5341 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     5401 caccattcga tggtgtcctg gcacgacagg tttcccga
//
