LOCUS       40924_13910        8457 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCWP2Fluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Dawson SC.
  TITLE     Giardia colonizes and encysts in high density foci in the murine 
            small intestine
  JOURNAL   mSphere (2017) In press
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   Dawson SC.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2017) Department of Microbiology and Molecular 
            Genetics, Uc Davis, One Shields Avenue, Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 8457)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8457)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere 
            (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-MAY-2017) Department of Microbiology and Molecular Genetics, Uc 
            Davis, One Shields Avenue, Davis, CA 95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    893..914
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        929..959
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    967..983
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     991..1007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1025..1043
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           1144..1639
                     /gene="CWP2"
     CDS             1645..3294
                     /label=luciferase
                     /note="firefly luciferase"
     3'UTR           3308..3413
                     /gene="CWP2"
     5'UTR           3978..4177
                     /gene="gamma giardin"
     gene            3978..4177
                     /gene="gamma giardin"
                     /label=gamma giardin
     CDS             4187..4780
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     3'UTR           4790..4906
                     /gene="GDH"
     gene            4790..4906
                     /gene="GDH"
                     /label=GDH
     misc_feature    complement(4913..5629)
                     /label=similar to aurora kinase
                     /note="similar to aurora kinase"
     CDS             complement(6361..6372)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     promoter        complement(6623..6641)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6648..6664)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6805..7260
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7338..7442
                     /label=AmpR promoter
     CDS             7443..8300
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      841 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      901 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      961 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     1021 agctatttag gtgacactat agaatactca agctatgcat ccaacgcgtt gggagctctc
     1081 ccatatggtc gacctgcagg cggccgccag atcttccgga tggctcgagt ttttcagcaa
     1141 gatacagttt attaatgatc ctctcaaaag tcatctccag aggctataca agtgcgacga
     1201 aggcagtaga aagaagcagt agaaagagaa gcaaagccat ttttcttttg gccgccaatt
     1261 cacacagctg tgcgctctat ggcaaacatg ggatgtgatc gatgtgacac gaaaaagtct
     1321 caaagaaatc tcaaagaagt ctcaaaggat ggtggtttac tactccacag atgtcttagt
     1381 ccactataaa ggatacccct tgggccttga agcacaactg tgaaccttag aaccacaagg
     1441 tgtttctaag taccagagtc acaagaggaa cacagagcca aaagcatcta caacccactt
     1501 tgatgagagc atgggttctg acaaatagtg actgtgaacg agaaaacctt aacttaagat
     1561 atgaactaaa aagaaacagt agactgatgc tgtagaggtc aacagataac agcagctcaa
     1621 cagtggctgg gaaaataaaa tgccatggaa gatgccaaaa acattaagaa gggcccagcg
     1681 ccattctacc cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc
     1741 tacgccctgg tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc
     1801 tacgccgagt acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg
     1861 aatacaaacc atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg
     1921 ttgggtgccc tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc
     1981 gagctgctga acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg
     2041 ctgcaaaaga tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg
     2101 gatagcaaga ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg
     2161 ccacccggct tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc
     2221 gccctgatca tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac
     2281 cgcaccgctt gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc
     2341 cccgacaccg ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg
     2401 ctgggctact tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta
     2461 ttcttgcgca gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt
     2521 agcttcttcg ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc
     2581 gccagcggcg gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac
     2641 ctaccaggca tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc
     2701 cccgaagggg acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag
     2761 gtggtggact tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc
     2821 cgtggcccca tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc
     2881 gacaaggacg gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc
     2941 ttcatcgtgg accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc
     3001 gaactggaga gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg
     3061 cccgacgacg atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc
     3121 atgaccgaga aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg
     3181 cgcggtggtg ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc
     3241 cgcaagatcc gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtgtaataa
     3301 ttctagagat gtgacaaagc tcatcctgtt tgctgctttg atccagaagt aaacatgcag
     3361 gtttctctta gctactcctg ttgaagctaa aattataaaa ggactcagca gggatggaat
     3421 gcgagctgca gccgcttttc gacgtagaag aaactggggt gcatgcgtat ctgctgacca
     3481 taaacgacac tcttcttctt gtcgactgtg gcctggctgc accctacgag acatatctcc
     3541 gagtgtatga tgaccacatc gataacatca gcgcaatact cctcacatcg gctgccttgg
     3601 aacacgcagg tgcctgcctg tatattttga gcagaaagga gatcaagaca tttataccca
     3661 ccaatcttgt tcccgcgctc cacgctactg tgttgaatgt tttcttcaat ttatgtcttg
     3721 atatgaaagg tcatcagcca gttctggact tcatcctctt agattacctc aagcttggaa
     3781 ttctttccgt cgacctttgc atcatcgttg ctcggtcgtc tgcctcgttt acgacctgct
     3841 tgttcctggg cctcaaatgg ttagtggttt tccttcttcc aacggcgttg gtccatcctc
     3901 ggccgcgtga tcaaatgcct tcgagcgtat gtctgacctg tttgtgaatg acgatccgtg
     3961 gctttactca gattcctgtg tttgtgaata agaaatccgt gaatttgttg gggtggaacg
     4021 gcgaagtggc ctctgctaca ataagaactg gagagtgtgt tcagaggcga atgcgcaacg
     4081 aactttgtcg ttacacgacg tgtgcgcctg tggtcttcgt gcggtttccg cgcggaattt
     4141 tgcgcctttt ctaaaaaact aaccctaaaa caagaaaatc atgggcaccg agtacaagcc
     4201 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     4261 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     4321 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4381 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4441 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4501 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4561 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     4621 cggagtggag gcggccgagc gcgccggggt gcctgccttc ctggagacct ccgcgccccg
     4681 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4741 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc ctcgagtgag ggctgaagtg
     4801 aatatttacc ttttccgggt tcgacgggcg tgcttgacga tgcggaagtc gctgtccgtt
     4861 ccaacggcta tctgccccag gacccccatc ccggcgccga tcagctggta ccgagctcat
     4921 agcacagggc tccgagtgac cagatatcgc aagccttatc ataagattgt ccgagcatga
     4981 cctccggggg aaggtagtcg agagttccgc actgggtgcg tcgtcggcca ccgtgggttg
     5041 gatggtgcac ggaccatcca aagtctgcaa tcttgtatgt gtagtgtgcc attcggacta
     5101 gctcgtgttc atgcacagaa atggagtcat ctgaattgtg cgcatctgtg tgctctttgg
     5161 tctctttccc cttgcaaatc aagatgtttt ctgccttaag gtccctatgg aagatgttct
     5221 ttctgtgaag atactgtatc gcctgtgcca cgtctcgaat aatcttgctt acaacgggta
     5281 gaggaaactt cccgaactgc tgtaaccttt ggtaaagctc gccgtacgga gccacttcga
     5341 ggaccaaaaa gactcgggtc tcgtcctgga accaagtata caagcgaatt atgtttatgt
     5401 gcctcacatt aagatgtata tccaattccc tgcgcacctg gccctcaaca cgctccgatt
     5461 tgatgtagga tttatacagc actttaaggg caaccaccaa cttggaggac tgttcacgtg
     5521 ctaggtaaac caagccatac tttccacgac caagaaagcg tccaatctca aagtcctcta
     5581 ttgtagtgcg ctttcctgtc cctgtgtggg gtaccaagtg ctgcggcatt ttttaagtta
     5641 tttacttgga gtgcgtcttg ttcttcttat cgtcatcctt gctcttatgc ttttccttgt
     5701 ctttttctct gtctttatcc ctgtgcttgt ccttgtcttt atgtttttcc ttatctcttt
     5761 ctttatcctt atccttgtcc ctgtgcttct ctttatcctt ctccttctcc ttgtgctttt
     5821 ctttttcttt gtccttgtct ctgtgctttt ccttgctctt ctccttatgc ttctccttag
     5881 cgtccttggt gtcattggtg tcttccgcat ccacctcaat tggtgccacg ggctcgtctt
     5941 tcttcgcagc actcgataaa ggctctaagg gcgctggcat agcatcgtct tgtcgttctt
     6001 gtgcattctc cttatttctt gagcccttat cctgtgaggc cttggtctgc gtgaacccag
     6061 tgtcttcctc aaacgcctcc gtcattcgag tctttttggg ctcagcagca ggggctgttt
     6121 ccttcgaaag cttcatgtcc cccttccctt catatcctgt tgccactccg cgcctgtatg
     6181 cttccatctt ctgtgaagca atagattttc tgtttgagcg agcttgcttg tccccttcat
     6241 agaagcgcag agacgcgttt atcttctcaa gctttccttt agcaaagctg gtgttagagg
     6301 actccgagag agcgtccaca cgagcagcaa gagatgcgtt tgttgctacg acacgtgcca
     6361 tcttccctcg attggttgca gatgcttttc ccacaagcgc agcgtgatag ataaacccat
     6421 atttgggcgt ctgcttggat cgcattttct tggcacggaa aagtgcacgt tctgagccaa
     6481 gaatctgcaa ggtggatgcg gggagcttgg caaggttgag caaacttccc gctttggcca
     6541 gaagacggac gccgacgatg gacccgacaa tgcgggtgaa attgggtgca acagcaagca
     6601 tgcgacgtcg ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
     6661 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     6721 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     6781 ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6841 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6901 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6961 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7021 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7081 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7141 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7201 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     7261 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca
     7321 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7381 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7441 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7501 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7561 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7621 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7681 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7741 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7801 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7861 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7921 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7981 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8041 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8101 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8161 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8221 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8281 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8341 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8401 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaa
//
