LOCUS       40924_13905        8083 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCWP1Fluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Dawson SC.
  TITLE     Giardia colonizes and encysts in high density foci in the murine 
            small intestine
  JOURNAL   mSphere (2017) In press
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Dawson SC.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2017) Department of Microbiology and Molecular 
            Genetics, Uc Davis, One Shields Avenue, Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 8083)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8083)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere 
            (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-MAY-2017) Department of Microbiology and Molecular Genetics, Uc 
            Davis, One Shields Avenue, Davis, CA 95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    893..914
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        929..959
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    967..983
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     991..1007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1025..1043
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           1122..1264
                     /gene="CWP1"
     gene            1122..1264
                     /gene="CWP1"
                     /label=CWP1
     CDS             1271..2920
                     /label=luciferase
                     /note="firefly luciferase"
     3'UTR           2934..3403
                     /gene="CWP2"
     gene            2934..3403
                     /gene="CWP2"
                     /label=CWP2
     5'UTR           3604..3803
                     /gene="gamma giardin"
     gene            3604..3803
                     /gene="gamma giardin"
                     /label=gamma giardin
     CDS             3813..4406
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     3'UTR           4416..4534
                     /gene="GDH"
     gene            4416..4534
                     /gene="GDH"
                     /label=GDH
     misc_feature    complement(4539..5255)
                     /label=similar to aurora kinase
                     /note="similar to aurora kinase"
     CDS             complement(5987..5998)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     promoter        complement(6249..6267)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6274..6290)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6431..6886
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6964..7068
                     /label=AmpR promoter
     CDS             7069..7926
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      841 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      901 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      961 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     1021 agctatttag gtgacactat agaatactca agctatgcat ccaacgcgtt gggagctctc
     1081 ccatatggtc gacctgcagg cggccgcact agtgattata agcttactaa atcattctct
     1141 tgttctagcc acgcatgggc tgtctgcatt ttttctggct aacagtctac agtctacaat
     1201 ttacagtata caatctacag tttacaactc tcagatcaag aaacacagaa ataaaatatc
     1261 agggatgacc atggaagatg ccaaaaacat taagaagggc ccagcgccat tctacccact
     1321 cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg ccctggtgcc
     1381 cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg ccgagtactt
     1441 cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata caaaccatcg
     1501 gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg gtgccctgtt
     1561 catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc tgctgaacag
     1621 catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct
     1681 caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata gcaagaccga
     1741 ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac ccggcttcaa
     1801 cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc tgatcatgaa
     1861 cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca ccgcttgtgt
     1921 ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg acaccgctat
     1981 cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg gctacttgat
     2041 ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct tgcgcagctt
     2101 gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct tcttcgctaa
     2161 gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca gcggcggggc
     2221 gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac caggcatccg
     2281 ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg aaggggacga
     2341 caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tggacttgga
     2401 caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat
     2461 catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca aggacggctg
     2521 gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg
     2581 gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagagcat
     2641 cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc
     2701 cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga
     2761 gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt
     2821 gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca agatccgcga
     2881 gattctcatt aaggccaaga agggcggcaa gatcgccgtg taataattct agagatgtga
     2941 caaagctcat cctgtttgct gctttgatcc agaagtaaac atgcaggttt ctcttagcta
     3001 ctcctgttga agctaaaatt ataaaaggac tcagcaggga tggaatgcga gctgcagccg
     3061 cttttcgacg tagaagaaac tggggtgcat gcgtatctgc tgaccataaa cgacactctt
     3121 cttcttgtcg actgtggcct ggctgcaccc tacgagacat atctccgagt gtatgatgac
     3181 cacatcgata acatcagcgc aatactcctc acatcggctg ccttggaaca cgcaggtgcc
     3241 tgcctgtata ttttgagcag aaaggagatc aagacattta tacccaccaa tcttgttccc
     3301 gcgctccacg ctactgtgtt gaatgttttc ttcaatttat gtcttgatat gaaaggtcat
     3361 cagccagttc tggacttcat cctcttagat tacctcaagc ttggaattct ttccgtcgac
     3421 ctttgcatca tcgttgctcg gtcgtctgcc tcgtttacga cctgcttgtt cctgggcctc
     3481 aaatggttag tggttttcct tcttccaacg gcgttggtcc atcctcggcc gcgtgatcaa
     3541 atgccttcga gcgtatgtct gacctgtttg tgaatgacga tccgtggctt tactcagatt
     3601 cctgtgtttg tgaataagaa atccgtgaat ttgttggggt ggaacggcga agtggcctct
     3661 gctacaataa gaactggaga gtgtgttcag aggcgaatgc gcaacgaact ttgtcgttac
     3721 acgacgtgtg cgcctgtggt cttcgtgcgg tttccgcgcg gaattttgcg ccttttctaa
     3781 aaaactaacc ctaaaacaag aaaatcatgg gcaccgagta caagcccacg gtgcgcctcg
     3841 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     3901 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     3961 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     4021 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     4081 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     4141 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     4201 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     4261 ccgagcgcgc cggggtgcct gccttcctgg agacctccgc gccccgcaac ctccccttct
     4321 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     4381 ggtgcatgac ccgcaagccc ggtgccctcg agtgagggct gaagtgaata tttacctttt
     4441 ccgggttcga cgggcgtgct tgacgatgcg gaagtcgctg tccgttccaa cggctatctg
     4501 ccccaggacc cccatcccgg cgccgatcag ctggtaccga gctcatagca cagggctccg
     4561 agtgaccaga tatcgcaagc cttatcataa gattgtccga gcatgacctc cgggggaagg
     4621 tagtcgagag ttccgcactg ggtgcgtcgt cggccaccgt gggttggatg gtgcacggac
     4681 catccaaagt ctgcaatctt gtatgtgtag tgtgccattc ggactagctc gtgttcatgc
     4741 acagaaatgg agtcatctga attgtgcgca tctgtgtgct ctttggtctc tttccccttg
     4801 caaatcaaga tgttttctgc cttaaggtcc ctatggaaga tgttctttct gtgaagatac
     4861 tgtatcgcct gtgccacgtc tcgaataatc ttgcttacaa cgggtagagg aaacttcccg
     4921 aactgctgta acctttggta aagctcgccg tacggagcca cttcgaggac caaaaagact
     4981 cgggtctcgt cctggaacca agtatacaag cgaattatgt ttatgtgcct cacattaaga
     5041 tgtatatcca attccctgcg cacctggccc tcaacacgct ccgatttgat gtaggattta
     5101 tacagcactt taagggcaac caccaacttg gaggactgtt cacgtgctag gtaaaccaag
     5161 ccatactttc cacgaccaag aaagcgtcca atctcaaagt cctctattgt agtgcgcttt
     5221 cctgtccctg tgtggggtac caagtgctgc ggcatttttt aagttattta cttggagtgc
     5281 gtcttgttct tcttatcgtc atccttgctc ttatgctttt ccttgtcttt ttctctgtct
     5341 ttatccctgt gcttgtcctt gtctttatgt ttttccttat ctctttcttt atccttatcc
     5401 ttgtccctgt gcttctcttt atccttctcc ttctccttgt gcttttcttt ttctttgtcc
     5461 ttgtctctgt gcttttcctt gctcttctcc ttatgcttct ccttagcgtc cttggtgtca
     5521 ttggtgtctt ccgcatccac ctcaattggt gccacgggct cgtctttctt cgcagcactc
     5581 gataaaggct ctaagggcgc tggcatagca tcgtcttgtc gttcttgtgc attctcctta
     5641 tttcttgagc ccttatcctg tgaggccttg gtctgcgtga acccagtgtc ttcctcaaac
     5701 gcctccgtca ttcgagtctt tttgggctca gcagcagggg ctgtttcctt cgaaagcttc
     5761 atgtccccct tcccttcata tcctgttgcc actccgcgcc tgtatgcttc catcttctgt
     5821 gaagcaatag attttctgtt tgagcgagct tgcttgtccc cttcatagaa gcgcagagac
     5881 gcgtttatct tctcaagctt tcctttagca aagctggtgt tagaggactc cgagagagcg
     5941 tccacacgag cagcaagaga tgcgtttgtt gctacgacac gtgccatctt ccctcgattg
     6001 gttgcagatg cttttcccac aagcgcagcg tgatagataa acccatattt gggcgtctgc
     6061 ttggatcgca ttttcttggc acggaaaagt gcacgttctg agccaagaat ctgcaaggtg
     6121 gatgcgggga gcttggcaag gttgagcaaa cttcccgctt tggccagaag acggacgccg
     6181 acgatggacc cgacaatgcg ggtgaaattg ggtgcaacag caagcatgcg acgtcgggcc
     6241 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
     6301 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     6361 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     6421 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6481 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6541 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6601 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6661 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6721 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6781 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6841 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt
     6901 ttctccttac gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat
     6961 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7021 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7081 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7141 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7201 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7261 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7321 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7381 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7441 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7501 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7561 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7621 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7681 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7741 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7801 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7861 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7921 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7981 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8041 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaa
//
