LOCUS       V015901                 9540 bp    DNA     circular SYN 06-JUL-2021
DEFINITION  Exported.
ACCESSION   V015901
VERSION     V015901
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Wang C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2020) Sichuan University, West China School of
            Public Health, Renmin Nan Road, Chengdu, Sichuan 610041, China
REFERENCE   2  (bases 1 to 9540)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (13-MAY-2020) Sichuan University, West China School of Public
            Health, Renmin Nan Road, Chengdu, Sichuan 610041, China"
FEATURES             Location/Qualifiers
     source          1..9540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             515..703
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     primer_bind     complement(700..722)
                     /label="pGEX_3 primer"
                     /note="pGEX_3 primer"
     misc_feature    808..948
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1134..1722)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1896..2753)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2754..2858)
                     /label="AmpR promoter"
     CDS             2908..3966
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase"
                     /label="zinc metalloproteinase"
                     /protein_id="QXF31294.1"
                     /translation="MTLTYKNRRITRPFRLQEFPAWSHTTVGGLPITEWISEDEQGAMD
                     TIFVSVRDAAYEIINKKGATFYGVAAALARITKAILNNENAILPLSVYLDGHYGMNDIY
                     IGAPAVVNRQGVRHIVEMNLNDKEKEQMKNSADTLKKVLDKGGEIDSFVHYGLNCNNAF
                     WDGQEILYGDGDKKNFKPFSCAKTIVGHELTHAVIQYSAGLEYEGQSGALNESFADVFG
                     YFIAPNHWLIGEDVCVRGSRDGRIRSIKDPDKYNQAAHMKDYESLPITEEGDWGGVHYN
                     SGIPNKAAYNTITKLGKEKTEQRRFRALKYYLTKKAQFTDAKKALQQAAKDLYGEDASK
                     KVAEAWEAVGVN"
     CDS             4344..4370
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             5334..5366
                     /label="VSV-G tag"
                     /note="epitope tag from vesicular stomatitis virus G
                     protein"
     CDS             complement(5783..6514)
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /label="DNA alkylation repair protein"
                     /protein_id="QXF31296.1"
                     /translation="MRLRMLFLLYLLYSRGVTRMITFDQLDTELQALENPNTIKIFRNH
                     GCPDSLDLYGLKIGDLKKIIRREKLTKNHELAVKLIESNNSDLIYLGLLAINPNKITTE
                     QIEKWNIAFRETWSQLTFGLASIVSKRDDALLFAKTWIESDYDLTKSMGWQIYSEHINN
                     LPEAETLLQRAKETLQTETNRTRYSMNGFIITCGIYKDDLHEKAMEAAKSVGKVHVNLG
                     NTACKVPDAISYIEKARNRTK"
     CDS             7220..7951
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
ORIGIN
        1 tcatgtttga cagcttatca tcgtcgggca gcgttgggtc ctggccacgg gtgcgcatga
       61 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
      121 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
      181 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
      241 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg
      301 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt
      361 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt
      421 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca
      481 tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa aaaccgccct
      541 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct
      601 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta
      661 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
      721 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
      781 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
      841 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
      901 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
      961 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1561 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1681 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1741 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1801 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1861 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1921 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1981 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2041 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2101 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2161 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     2221 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2281 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2341 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2401 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2461 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     2521 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2581 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2641 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2701 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2881 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2941 aggccctttc gtcttcaaga attcccagca tggagccaca caactgtcgg cggccttcca
     3001 attactgaat ggattagcga agatgaacaa ggtgcaatgg atactatttt cgtaagtgtt
     3061 cgtgatgcag cttatgaaat tattaataaa aaaggcgcta cattctacgg cgttgctgca
     3121 gctcttgctc gtattacaaa agcaattcta aataacgaaa atgcgatttt gccactttct
     3181 gtttatttag atggccatta cggtatgaac gatatttata taggtgcacc agcagtcgtt
     3241 aaccgtcaag gcgttcgcca tattgttgaa atgaacttaa atgacaaaga aaaagaacaa
     3301 atgaaaaact ccgcagatac acttaaaaaa gttctagata aaggtggaga aattgattcg
     3361 tttgtccatt atggcttgaa ttgcaataat gccttttggg atggccaaga aattctttat
     3421 ggagatgggg acaaaaagaa tttcaaacca ttttcatgcg ccaaaactat tgttggtcat
     3481 gaactaacgc atgcagttat ccagtattcg gcgggattgg aatacgaagg gcaatcaggt
     3541 gcgctaaacg agtcgttcgc cgatgttttt ggttatttta ttgcgccaaa tcattggttg
     3601 attggtgagg atgtctgtgt gcgtgggtcg cgagatgggc gaataagaag cattaaagat
     3661 cctgacaaat ataatcaagc ggctcatatg aaggattacg aatcgcttcc aatcacagag
     3721 gaaggcgact ggggcggagt tcattataat agtggtatcc cgaataaagc agcctataat
     3781 actatcacta aacttggaaa agaaaaaaca gaacagcgac gatttcgcgc cttaaagtac
     3841 tatttaacga aaaaagccca gtttaccgat gcgaaaaaag cgcttcaaca agcagcgaaa
     3901 gatttatatg gtgaagatgc ttctaaaaaa gttgctgaag cttgggaagc ggtaggagtt
     3961 aactgagcgg ccgccagtgt gatggatatc tgcagaatta attcggcttt ctagagtgac
     4021 ttttatgttg aggcattaac atttgttaac gacgataaag ggacagcagg actagaataa
     4081 agctataaag caagcatata atattgcgtt tcatctttag aagcgaattt cgccaatatt
     4141 ataattatca aaagagaggg gtggcaaacg gtatttggca ttattaggtt aaaaaatgta
     4201 gaaggagagt gaaacccatg aaaaaaataa tgctagtttt tattacactt atattagtta
     4261 gtctaccaat tgcgcaacaa actgaagcaa aggatgcatc ggatcttaaa gctgtttata
     4321 attttgctac tatgaaggat ccatatccat atgatgttcc agattatgca tcattgaatt
     4381 caatgagaag tgaaagacca caagctttgc atcaaaaacg tacagcaatg ttccaagatc
     4441 cacaagaatc tggtcgtaaa ttaccacaat tatgtacaga attacaaaca acaattcatg
     4501 atattatttt agaatgtgtt tattgtaaac aacaattatt acgtcgtgaa gtttatgatt
     4561 tcgcattccg tgatttatgt attgtttatc gtgatggtaa cccatatgca gtttgtgata
     4621 aatgtttaaa attctattct aaaatttctg aatatcgtca tcatggtgat acaccaacat
     4681 tacatgaata tatgttagat ttacaaccag aaacaacaga tttatatggt tatggtcaat
     4741 taaacgattc ttctgaagaa gaagatgaaa ttgatggtcc agcaggtcaa gcagaaccag
     4801 atcgtgcaca ttataacatt gttacattct gttgtaaatg tgattctaca ttagataaat
     4861 gtttaaaatt ctattctaaa atttctgaat atcgtcatta ttgttattct gtttatggta
     4921 caacattaga acaacaatat aacaaaccat tatgtgattt attaattcgt tgtattaact
     4981 gtcaaaaacc attatgtcca gaagaaaaac aacgtcattt agataaaaaa caacgtttcc
     5041 ataacattcg tggtcgttgg acaggtcgtt gtatgtcttg ttgtcgttct tctcgtacac
     5101 gtcgtgaaac acaattacat tataacattg ttacattctg ttgtaaatgt gattctacat
     5161 tacgtttatg tgttcaatct acacatgttg atattcgtac attagaagat ttattaatgg
     5221 gtacattagg tattgtttgt ccaatttgtt ctcaaaaacc actcgaggga ttaaatggac
     5281 cagatattta taaaggagtt tatcaattta aaagtgttga atttgatgtc gagtatacag
     5341 atattgaaat gaatagatta ggaaaatgat aagtcggcgg ccgctaataa tcttgcgctt
     5401 cgatgacaac agctgtacca gatgcagtga ccattagcat tccgttatca gctccaagca
     5461 cttcataatc aatatcaaca ccgataacgg catttgcgcc aatatctttc gcacgttgtt
     5521 ccatttcacg aattgcttcc tcgcgagcat taataagttc atcttcatag ccttgcgaac
     5581 ggcccccgaa gaaatttcga agtccagccc caatatcttt cataaagtta acgccagtga
     5641 tgacttcgcc gaaaacgatt tttttatatt cgataatttg tttgccttca atatttggtg
     5701 aagttgttac aatcatgagt tatccctaca gtttttcttt tatcatacct cttagtactt
     5761 tttctagtca aaggatatcc ggttatttcg tacgatttcg cgctttttct atataagaaa
     5821 tagcatctgg aactttacaa gctgtatttc caaggtttac atgaactttc ccgactgatt
     5881 tcgcggcttc catcgctttt tcgtgcaaat cgtctttgta aattccacaa gtgataataa
     5941 aaccgttcat tgaatagcgg gttctattgg tttctgtttg cagcgtttct ttcgcgcgtt
     6001 gaagtaatgt ctctgcttca ggcaaattgt taatatgttc gctgtaaatc tgccagccca
     6061 tggatttagt taaatcataa tcactttcaa tccatgtttt ggcgaaaagg agtgcatcat
     6121 cacgtttgct tacaatcgat gctagtccaa aagttagttg tgaccatgtt tcgcgaaaag
     6181 cgatattcca tttttcgatt tgttcggtgg ttattttgtt tggattaatt gctagcaaac
     6241 cgagataaat caagtcacta ttatttgatt caatcagttt tacggcgagt tcgtgatttt
     6301 ttgtcagttt ttctcggcgg atgattttct ttaaatcacc aatttttagt ccgtaaagat
     6361 ctaatgaatc cggacaaccg tgattacgaa aaattttgat cgtattgggg ttttctaagg
     6421 cttgtagctc ggtgtcaagt tggtcaaaag taatcatacg cgtcactcct ctcgaataaa
     6481 gtaagtataa caaaaaaagc atgcgaaggc gcatgcttta ggatttaaga atattagtct
     6541 atttgtttca ttgcgtcgta ctagtcccgg ggcgaattgc atctgaccga attttacgtt
     6601 tccctgaata attctcatca atcgtttcat caattttatc tttatacttt atattttgtg
     6661 cgttaatcaa atcataattt ttatatgttt cctcatgatt tatgtcttta ttattatagt
     6721 ttttattctc tctttgatta tgtctttgta tcccgtttgt attacttgat cctttaactc
     6781 tggcaaccct caaaattgaa tgagacatgc tacacctccg gataataaat atatataaac
     6841 gtataaagat ttcataaagt ctaacacact agacttattt acttcgtaat taagtcgtta
     6901 aaccgtgtgc tctacgacca aaactataaa acctttaaga actttctttt tttacaagaa
     6961 aaaagaaatt agataaatct ctcatatctt ttattcaata atcgcatccg attgcagtat
     7021 aaatttaacg atcactcatc atgttcatat ttatcagagc tcgtgctata attatactaa
     7081 ttttataagg aggaaaaaat atgggcattt ttagtatttt tgtaatcagc acagttcatt
     7141 atcaaccaaa caaaaaataa gtggttataa tgaatcgtta ataagcaaaa ttcatataac
     7201 caaattaaag agggttataa tgaacgagaa aaatataaaa cacagtcaaa actttattac
     7261 ttcaaaacat aatatagata aaataatgac aaatataaga ttaaatgaac atgataatat
     7321 ctttgaaatc ggctcaggaa aaggccattt tacccttgaa ttagtaaaga ggtgtaattt
     7381 cgtaactgcc attgaaatag accataaatt atgcaaaact acagaaaata aacttgttga
     7441 tcacgataat ttccaagttt taaacaagga tatattgcag tttaaatttc ctaaaaacca
     7501 atcctataaa atatatggta atatacctta taacataagt acggatataa tacgcaaaat
     7561 tgttcttgat agtatagcta atgagattta tttaatcgtg gaatacgggt ttgctaaaag
     7621 attattaaat acaaaacgct cattggcatt acttttaatg gcagaagttg atatttctat
     7681 attaagtatg gttccaagag aatattttca tcctaaacct aaagtgaata gctcacttat
     7741 cagattaagt agaaaaaaat caagaatatc acacaaagat aaacaaaagt ataattattt
     7801 cgttctgaaa tgggttaaca aagaatacaa gaaaatattt acaaaaaatc aatttaacaa
     7861 ttccttaaaa catgcaggaa ttgacgattt aaacaatatt agctttgaac aattcttatc
     7921 tcttgtcaat agctataaat tatttaataa gtaagttaag ggatgcataa actgcatccc
     7981 ttaacttgtt tttcgtgtgc ctattttttg tgaatcgtcg acaagcttta atgcggtagt
     8041 ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc
     8101 atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta
     8161 ctgccgggcc tcttgcggga tcgatagaaa gcgtgagaaa cagcgtacag acgatttaga
     8221 gatgtagagg tacttttatg ccgagaaaac tttttgcgtg tgacagtcct taaaatatac
     8281 ttagagcgta agcgaaagta gtagcgacag ctattaactt tcggttgcaa agctctagga
     8341 tttttaatgg acgcagcgca tcacacgcaa aaaggaaatt ggaataaatg cgaaatttga
     8401 gatgttaatt aaagaccttt ttgaggtctt tttttcttag atttttgggg ttatttaggg
     8461 gagaaaacat aggggggtac tacgacctcc cccctaggtg tccattgtcc attgtccaaa
     8521 caaataaata aatattgggt ttttaatgtt aaaaggttgt tttttatgtt aaagtgaaaa
     8581 aaacagatgt tgggaggtac agtgatagtt gtagatagaa aagaagagaa aaaagttgct
     8641 gttactttaa gacttacaac agaagaaaat gagatattaa atagaatcaa agaaaaatat
     8701 aatattagca aatcagatgc aaccggtatt ctaataaaaa aatatgcaaa ggaggaatac
     8761 ggtgcatttt aaacaaaaaa agatagacag cactggcatg ctgcctatct atgactaaat
     8821 tttgttaagt gtattagcac cgttattata tcatgagcga aaatgtaata aaagaaactg
     8881 aaaacaagaa aaattcaaga ggacgtaatt ggacatttgt tttatatcca gaatcagcaa
     8941 agccgagtgg ttagagtatt taaaagagtt acacattcaa tttgtagtgc tccattacat
     9001 gatagggata ctgatacaga aggtaggatg aaaaaagagc attatcatat tctagtgatg
     9061 tatgagggta ataaatctta tgaacagata aaaataataa cagaagaatt gaatgcgact
     9121 attccgcaga ttgcaggaag tgtgaaaggt cttgtgagat atatgcttca catggacgat
     9181 cctaataaat ttaaatatca aaaagaagat atgatagttt atggcggtgt agatgttgat
     9241 gaattattaa agaaaacaac aacagataga tataaattaa ttaaagaaat gattgagttt
     9301 attgatgaac aaggaatcgt agaatttaag agtttaatgg attatgcaat gaagtttaaa
     9361 tttgatgatt ggttcccgct tttatgtgat aactcggcgt atgttattca agaatatata
     9421 aaatcaaatc ggtataaatc tgaccgatag attttgaatt taggtgtcac aagacactct
     9481 tttttcgcac cagcgaaaac tggtttaagc cgagtgcgca aaagacataa tcgggaattc
//
