LOCUS       V016124                 8159 bp    DNA     circular SYN 20-JUN-2020
DEFINITION  Exported.
ACCESSION   V016124
VERSION     V016124
KEYWORDS    UNVERIFIED
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Yuzhen Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2019) Department of Public Health Laboratory
            Sciences, West China School of Public HealthSichuan University, No.
            17, Section 3, Renmin South Road, Wuhou District, Chengdu, Sichuan
            610041, China
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (02-OCT-2019) Department of Public Health Laboratory Sciences, West
            China School of Public HealthSichuan University, No. 17, Section 3,
            Renmin South Road, Wuhou District, Chengdu, Sichuan 610041, China"
            GenBank staff is unable to verify sequence and/or annotation
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..8159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             515..703
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    808..948
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1134..1722)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1896..2753)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2754..2858)
                     /label="AmpR promoter"
     CDS             5839..6570
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
ORIGIN
        1 tcatgtttga cagcttatca tcgtcgggca gcgttgggtc ctggccacgg gtgcgcatga
       61 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
      121 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
      181 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
      241 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg
      301 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt
      361 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt
      421 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca
      481 tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa aaaccgccct
      541 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct
      601 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta
      661 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
      721 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
      781 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
      841 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
      901 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
      961 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1561 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1681 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1741 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1801 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1861 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1921 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1981 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2041 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2101 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2161 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     2221 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2281 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2341 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2401 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2461 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     2521 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2581 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2641 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2701 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2881 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2941 aggccctttc gtcttcaaga attcccagca tggagccaca caactgtcgg cggccttcca
     3001 attactgaat ggattagcga agatgaacaa ggtgcaatgg atactatttt cgtaagtgtt
     3061 cgtgatgcag cttatgaaat tattaataaa aaaggcgcta cattctacgg cgttgctgca
     3121 gctcttgctc gtattacaaa agcaattcta aataacgaaa atgcgatttt gccactttct
     3181 gtttatttag atggccatta cggtatgaac gatatttata taggtgcacc agcagtcgtt
     3241 aaccgtcaag gcgttcgcca tattgttgaa atgaacttaa atgacaaaga aaaagaacaa
     3301 atgaaaaact ccgcagatac acttaaaaaa gttctagaca ttatcgctgc cacacatgat
     3361 gcgttaattc atttattagc agaaacaacg acagaaccat tcgcgggaca accgcttacg
     3421 acccttgcga ccgtttcgtt agcatataat gaacaagacg taccgatttt acctgatgga
     3481 acaggctatc tagttgccag aaccgcacca tataaaacca ctgcttgtac atgggtgcag
     3541 aaaaaatggc cgcatatggt accaaagaac aaaatgttac tacgaggctt tgttgggaaa
     3601 gcaggagaaa cttggttaga gcaagcaagc gatgaagcaa tcgtctcagc tgttttgaga
     3661 gattatgcgg aaattatgga ccttcacgcc gcgccacttt tttatgaagt aagccgaatg
     3721 aaatccgcga tgcctcaata cttagttaat caccaggacc gactaaaaca attgaaaaag
     3781 aacatcaaaa cagattaccc aggtgtttat cttgcaggaa tgagctacga aggtgtgggt
     3841 attcctgatt gcatcgcagg ggctcaaaca gccgctaagg aattagtaga ttacttggaa
     3901 gaggtgtaac atgattaaag gaattggtct agatatgatt gatttagaac gtgtaaaaca
     3961 agtcgtggaa aagaatccgc gctttatcga acgcgtttta acagaaaaag aaattaagca
     4021 atttgaaaaa tacgaaggta atcgcaaaat cgaattttta gctgggcgtt ttgcagcaaa
     4081 agaagcatat gctaaggcga atggaactgg ttttggcaaa catttaagtt ttacggatgt
     4141 cgaaattttg caagtagaag atggtcgccc ccatgttact ttaccagtca aatcaggcga
     4201 aaacgttttt gttagcatca cccacacagc tagatcagcc gcagctcaag tgataattga
     4261 aatttagaaa ggaaagtgaa caatcaaggt aatattcgtg ctttttttag aagaagcgtt
     4321 tataatctaa catatgaaac tttatagcta tatataggac ttaagggagt atatcagcta
     4381 gaactgtaat attcttaaca ataaaatgcg aaataactct tttcttcaag gcgtgataat
     4441 ggtacgatat agttgttact tacaggtacg aggctttggg ggtgtgacac gtgttagaga
     4501 aagaaaatcg gatgataata tccgtagaac tgacacagga aatgatacaa gaactcgacg
     4561 tagttgtaga aaaagaaaaa atggggcgga gtgaagttat aatggaagca acgcaacagt
     4621 ttttacaaga gaaaagggct cgcgaattaa gagacgagat ggaacgcggc tatgcagaga
     4681 tggcgacaat taattttgct atcgcatgcg agtgtaccca tgtcgaagca gaagcagaag
     4741 acaggaatat tagtatttta ggaggttaat ggctgatggt gaagcgtggt gacgtatact
     4801 acgcggacct ttcccccgtg gtcggaagcg agcaaggggg aatacggcct gttctcatca
     4861 ttcaaaatga tattggtaat agattcagtc caactgtgat cgtggcagca ataactgcaa
     4921 aaattcaaaa agcaaaattg ccaacgcacg tggaagctac tcgtaaagat ggctttgaga
     4981 gagattctgt cattctttta gaacagatta gaacgattga taaacagcgc ctgacagata
     5041 aaattacgca tttggacgaa gatttaatgg ccaaggtaaa caaggcactt gaagtcagtc
     5101 taggagtagt agaattttaa tccatcctaa aaaaaataac ataaaatgat gaatgcaggg
     5161 caaaagggtc tgacgtaaac tagtcccggg gcgaattgca tctgaccgaa ttttacgttt
     5221 ccctgaataa ttctcatcaa tcgtttcatc aattttatct ttatacttta tattttgtgc
     5281 gttaatcaaa tcataatttt tatatgtttc ctcatgattt atgtctttat tattatagtt
     5341 tttattctct ctttgattat gtctttgtat cccgtttgta ttacttgatc ctttaactct
     5401 ggcaaccctc aaaattgaat gagacatgct acacctccgg ataataaata tatataaacg
     5461 tataaagatt tcataaagtc taacacacta gacttattta cttcgtaatt aagtcgttaa
     5521 accgtgtgct ctacgaccaa aactataaaa cctttaagaa ctttcttttt ttacaagaaa
     5581 aaagaaatta gataaatctc tcatatcttt tattcaataa tcgcatccga ttgcagtata
     5641 aatttaacga tcactcatca tgttcatatt tatcagagct cgtgctataa ttatactaat
     5701 tttataagga ggaaaaaata tgggcatttt tagtattttt gtaatcagca cagttcatta
     5761 tcaaccaaac aaaaaataag tggttataat gaatcgttaa taagcaaaat tcatataacc
     5821 aaattaaaga gggttataat gaacgagaaa aatataaaac acagtcaaaa ctttattact
     5881 tcaaaacata atatagataa aataatgaca aatataagat taaatgaaca tgataatatc
     5941 tttgaaatcg gctcaggaaa aggccatttt acccttgaat tagtaaagag gtgtaatttc
     6001 gtaactgcca ttgaaataga ccataaatta tgcaaaacta cagaaaataa acttgttgat
     6061 cacgataatt tccaagtttt aaacaaggat atattgcagt ttaaatttcc taaaaaccaa
     6121 tcctataaaa tatatggtaa tataccttat aacataagta cggatataat acgcaaaatt
     6181 gttcttgata gtatagctaa tgagatttat ttaatcgtgg aatacgggtt tgctaaaaga
     6241 ttattaaata caaaacgctc attggcatta cttttaatgg cagaagttga tatttctata
     6301 ttaagtatgg ttccaagaga atattttcat cctaaaccta aagtgaatag ctcacttatc
     6361 agattaagta gaaaaaaatc aagaatatca cacaaagata aacaaaagta taattatttc
     6421 gttctgaaat gggttaacaa agaatacaag aaaatattta caaaaaatca atttaacaat
     6481 tccttaaaac atgcaggaat tgacgattta aacaatatta gctttgaaca attcttatct
     6541 cttgtcaata gctataaatt atttaataag taagttaagg gatgcataaa ctgcatccct
     6601 taacttgttt ttcgtgtgcc tattttttgt gaatcgtcga caagctttaa tgcggtagtt
     6661 tatcacagtt aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca
     6721 tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac
     6781 tgccgggcct cttgcgggat cgatagaaag cgtgagaaac agcgtacaga cgatttagag
     6841 atgtagaggt acttttatgc cgagaaaact ttttgcgtgt gacagtcctt aaaatatact
     6901 tagagcgtaa gcgaaagtag tagcgacagc tattaacttt cggttgcaaa gctctaggat
     6961 ttttaatgga cgcagcgcat cacacgcaaa aaggaaattg gaataaatgc gaaatttgag
     7021 atgttaatta aagacctttt tgaggtcttt ttttcttaga tttttggggt tatttagggg
     7081 agaaaacata ggggggtact acgacctccc ccctaggtgt ccattgtcca ttgtccaaac
     7141 aaataaataa atattgggtt tttaatgtta aaaggttgtt ttttatgtta aagtgaaaaa
     7201 aacagatgtt gggaggtaca gtgatagttg tagatagaaa agaagagaaa aaagttgctg
     7261 ttactttaag acttacaaca gaagaaaatg agatattaaa tagaatcaaa gaaaaatata
     7321 atattagcaa atcagatgca accggtattc taataaaaaa atatgcaaag gaggaatacg
     7381 gtgcatttta aacaaaaaaa gatagacagc actggcatgc tgcctatcta tgactaaatt
     7441 ttgttaagtg tattagcacc gttattatat catgagcgaa aatgtaataa aagaaactga
     7501 aaacaagaaa aattcaagag gacgtaattg gacatttgtt ttatatccag aatcagcaaa
     7561 gccgagtggt tagagtattt aaaagagtta cacattcaat ttgtagtgct ccattacatg
     7621 atagggatac tgatacagaa ggtaggatga aaaaagagca ttatcatatt ctagtgatgt
     7681 atgagggtaa taaatcttat gaacagataa aaataataac agaagaattg aatgcgacta
     7741 ttccgcagat tgcaggaagt gtgaaaggtc ttgtgagata tatgcttcac atggacgatc
     7801 ctaataaatt taaatatcaa aaagaagata tgatagttta tggcggtgta gatgttgatg
     7861 aattattaaa gaaaacaaca acagatagat ataaattaat taaagaaatg attgagttta
     7921 ttgatgaaca aggaatcgta gaatttaaga gtttaatgga ttatgcaatg aagtttaaat
     7981 ttgatgattg gttcccgctt ttatgtgata actcggcgta tgttattcaa gaatatataa
     8041 aatcaaatcg gtataaatct gaccgataga ttttgaattt aggtgtcaca agacactctt
     8101 ttttcgcacc agcgaaaact ggtttaagcc gagtgcgcaa aagacataat cgggaattc
//
