LOCUS       V016245                 8713 bp    DNA     circular SYN 20-JUN-2020
DEFINITION  Exported.
ACCESSION   V016245
VERSION     V016245
KEYWORDS    UNVERIFIED
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Yuzhen Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2019) Department of Public Health Laboratory
            Sciences, West China School of Public HealthSichuan University, No.
            17, Section 3, Renmin South Road, Wuhou District, Chengdu, Sichuan
            610041, China
REFERENCE   2  (bases 1 to 8713)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (02-OCT-2019) Department of Public Health Laboratory Sciences, West
            China School of Public HealthSichuan University, No. 17, Section 3,
            Renmin South Road, Wuhou District, Chengdu, Sichuan 610041, China"
            GenBank staff is unable to verify sequence and/or annotation
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..8713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             515..703
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    808..948
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1134..1722)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1896..2753)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2754..2858)
                     /label="AmpR promoter"
     CDS             6393..7124
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
ORIGIN
        1 tcatgtttga cagcttatca tcgtcgggca gcgttgggtc ctggccacgg gtgcgcatga
       61 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
      121 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
      181 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
      241 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg
      301 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt
      361 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt
      421 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca
      481 tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa aaaccgccct
      541 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct
      601 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta
      661 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
      721 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
      781 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
      841 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
      901 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
      961 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1561 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1681 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1741 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1801 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1861 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1921 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1981 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2041 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2101 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2161 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     2221 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2281 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2341 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2401 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2461 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     2521 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2581 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2641 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2701 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2881 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2941 aggccctttc gtcttcaaga attcccagca tggagccaca caactgtcgg cggccttcca
     3001 attactgaat ggattagcga agatgaacaa ggtgcaatgg atactatttt cgtaagtgtt
     3061 cgtgatgcag cttatgaaat tattaataaa aaaggcgcta cattctacgg cgttgctgca
     3121 gctcttgctc gtattacaaa agcaattcta aataacgaaa atgcgatttt gccactttct
     3181 gtttatttag atggccatta cggtatgaac gatatttata taggtgcacc agcagtcgtt
     3241 aaccgtcaag gcgttcgcca tattgttgaa atgaacttaa atgacaaaga aaaagaacaa
     3301 atgaaaaact ccgcagatac acttaaaaaa gttctagagc ttatagagcc acttttatct
     3361 ggtatttatg caggagatat ttataaaatg agcttgcggg caacatttcc gcaatttgaa
     3421 gaagcagtgg ctaaacacgg tagtttgatg gaaggcctgc aacaaaacca gctgaacacg
     3481 accggaacca aagcaactat tggtgcattc aggacgctag atggcgggct agaagcttta
     3541 ccaaaagcag ttgaacaagc tcttcctaag aaccatttgc acacaggaaa acaagcaacg
     3601 caaattttga aaaaaggtca ttcttatgaa attttttttg aaaatggaga taaaatatct
     3661 gctgatggcg tcattattgc tgctactcac gatacattaa ttgatttact tggagccagg
     3721 gtaacagaac cgtttgccaa ccagccatta accagccttg caacagtctc attagcttat
     3781 aatgaaagtg atgtacccat tttaccggat ggaacgggtt atttagttgc tagaactgct
     3841 ccttatcgaa ccactgcttg tacatgggtg caaaaaaaat ggccacatat ggtacctgaa
     3901 aataaaatgc tactacgagg ttttgttggt aaagcaggtg aaacttggtt agaagaggca
     3961 agtgatgaag caattatttc tgctgtttta gcagattatg ccgaaatgat ggatatcgaa
     4021 agagcgccac ttttttatga agtaagtcgg atgaagtctg caatgccaca gtatttagtg
     4081 acacaccaag ctagactaaa acaactaaaa gaaaatattg cgacagacta cccgggaatt
     4141 tattttgcag gaatgagtta tcaaggtgtt ggaattcctg attgtattgc tggggcgaaa
     4201 ctagctgtta atgacgttac tgattttttg aaagaggtgt aagatgatta aaggtattgg
     4261 gcttgatatg attgatttag cacgagtaaa acaagtttta gaaaagaatc cccgttttat
     4321 ggagcggatt ttaacagaaa aagaaattag ccaatttgaa aaatatgaag gtagtcgtaa
     4381 aattgaattt ttagcggggc gttttgctgc caaagaagct tatgcaaaag cgaacggaac
     4441 tggatttgga aaacatttaa gctttacgga tgtagaaatt ttacaagtag aagatggcag
     4501 acctcacgtg acaatgccga tcaaacctgg tgagtcggtg tttgtgagta ttacccacac
     4561 ggcgagatct gctgcggcac aagtgattat tgaagtttag aaaggaatgt ggaaattaag
     4621 gtaatattcg tgcttttttg aggagaagcg tttataatct aacatatgaa actttatagc
     4681 tacatctagg acttaaggga ggatgttgga taaaactata acaatcttaa caagtaaatg
     4741 ctaaataact cttttcttca tggcttgata atggtacgat atagttgtta cttacaggta
     4801 cgaggctttg ggggtgtgac acgtgttaga gaaagaaaag cggatgataa tatccgtaga
     4861 actgacacaa gaaatggtac aggaactcga cgtagttgta gaaaaagaaa aaatggggcg
     4921 gagtgaagtt ataatggaag caacgcaaca atttttgcaa gagaaaaggg cacgtgagtt
     4981 gagagacgag atggagcgcg gctatgcgga aatggcgaca attaactttg ctatcgcatg
     5041 cgagtgtacc catgtcgaat cagaagcaga agacagaaat attagtattt taggaggtta
     5101 atggctgatg gtgaagcgtg gtgacgtata ctacgcggac ctttcccccg tggtcggaag
     5161 cgagcaaggg ggaatacggc ctgttctcat cattcaaaac gacattggta atagattcag
     5221 cccaactgtg attgtggcag caataaccgc aaagattcaa aaagcaaagc tgccaacaca
     5281 tgtggaagct actcgtaaag atggctttga aagagattct gtcattcttt tagaacaaat
     5341 tagaacaatt gacaaacaac gcctgacaga taagattaca catttggacg aggaactaat
     5401 ggtcaaggta aaccaggcgc tggaagtcag tctaggagta gtagaattct aaacaaccta
     5461 aaaatataac atagaatgat gaacgcagga tagtaaataa gtccgacgta gaagaatacc
     5521 aaatacataa tgaactaatt aaccacttta caagatttaa gggacatcag aaacctacaa
     5581 acaatgcgta tgattcatcc ttcgctaaag ttgtttctaa tgtctttttt taaaaagtag
     5641 ttaatcaaat cgcagaaaaa aagagagatc tttaaggtgg agaaaggttg gaaaataatc
     5701 aatgtataaa gattttgcaa acttcatccg gaactagtcc cggggcgaat tgcatctgac
     5761 cgaattttac gtttccctga ataattctca tcaatcgttt catcaatttt atctttatac
     5821 tttatatttt gtgcgttaat caaatcataa tttttatatg tttcctcatg atttatgtct
     5881 ttattattat agtttttatt ctctctttga ttatgtcttt gtatcccgtt tgtattactt
     5941 gatcctttaa ctctggcaac cctcaaaatt gaatgagaca tgctacacct ccggataata
     6001 aatatatata aacgtataaa gatttcataa agtctaacac actagactta tttacttcgt
     6061 aattaagtcg ttaaaccgtg tgctctacga ccaaaactat aaaaccttta agaactttct
     6121 ttttttacaa gaaaaaagaa attagataaa tctctcatat cttttattca ataatcgcat
     6181 ccgattgcag tataaattta acgatcactc atcatgttca tatttatcag agctcgtgct
     6241 ataattatac taattttata aggaggaaaa aatatgggca tttttagtat ttttgtaatc
     6301 agcacagttc attatcaacc aaacaaaaaa taagtggtta taatgaatcg ttaataagca
     6361 aaattcatat aaccaaatta aagagggtta taatgaacga gaaaaatata aaacacagtc
     6421 aaaactttat tacttcaaaa cataatatag ataaaataat gacaaatata agattaaatg
     6481 aacatgataa tatctttgaa atcggctcag gaaaaggcca ttttaccctt gaattagtaa
     6541 agaggtgtaa tttcgtaact gccattgaaa tagaccataa attatgcaaa actacagaaa
     6601 ataaacttgt tgatcacgat aatttccaag ttttaaacaa ggatatattg cagtttaaat
     6661 ttcctaaaaa ccaatcctat aaaatatatg gtaatatacc ttataacata agtacggata
     6721 taatacgcaa aattgttctt gatagtatag ctaatgagat ttatttaatc gtggaatacg
     6781 ggtttgctaa aagattatta aatacaaaac gctcattggc attactttta atggcagaag
     6841 ttgatatttc tatattaagt atggttccaa gagaatattt tcatcctaaa cctaaagtga
     6901 atagctcact tatcagatta agtagaaaaa aatcaagaat atcacacaaa gataaacaaa
     6961 agtataatta tttcgttctg aaatgggtta acaaagaata caagaaaata tttacaaaaa
     7021 atcaatttaa caattcctta aaacatgcag gaattgacga tttaaacaat attagctttg
     7081 aacaattctt atctcttgtc aatagctata aattatttaa taagtaagtt aagggatgca
     7141 taaactgcat cccttaactt gtttttcgtg tgcctatttt ttgtgaatcg tcgacaagct
     7201 ttaatgcggt agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc
     7261 taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt
     7321 ggttatgccg gtactgccgg gcctcttgcg ggatcgatag aaagcgtgag aaacagcgta
     7381 cagacgattt agagatgtag aggtactttt atgccgagaa aactttttgc gtgtgacagt
     7441 ccttaaaata tacttagagc gtaagcgaaa gtagtagcga cagctattaa ctttcggttg
     7501 caaagctcta ggatttttaa tggacgcagc gcatcacacg caaaaaggaa attggaataa
     7561 atgcgaaatt tgagatgtta attaaagacc tttttgaggt ctttttttct tagatttttg
     7621 gggttattta ggggagaaaa catagggggg tactacgacc tcccccctag gtgtccattg
     7681 tccattgtcc aaacaaataa ataaatattg ggtttttaat gttaaaaggt tgttttttat
     7741 gttaaagtga aaaaaacaga tgttgggagg tacagtgata gttgtagata gaaaagaaga
     7801 gaaaaaagtt gctgttactt taagacttac aacagaagaa aatgagatat taaatagaat
     7861 caaagaaaaa tataatatta gcaaatcaga tgcaaccggt attctaataa aaaaatatgc
     7921 aaaggaggaa tacggtgcat tttaaacaaa aaaagataga cagcactggc atgctgccta
     7981 tctatgacta aattttgtta agtgtattag caccgttatt atatcatgag cgaaaatgta
     8041 ataaaagaaa ctgaaaacaa gaaaaattca agaggacgta attggacatt tgttttatat
     8101 ccagaatcag caaagccgag tggttagagt atttaaaaga gttacacatt caatttgtag
     8161 tgctccatta catgataggg atactgatac agaaggtagg atgaaaaaag agcattatca
     8221 tattctagtg atgtatgagg gtaataaatc ttatgaacag ataaaaataa taacagaaga
     8281 attgaatgcg actattccgc agattgcagg aagtgtgaaa ggtcttgtga gatatatgct
     8341 tcacatggac gatcctaata aatttaaata tcaaaaagaa gatatgatag tttatggcgg
     8401 tgtagatgtt gatgaattat taaagaaaac aacaacagat agatataaat taattaaaga
     8461 aatgattgag tttattgatg aacaaggaat cgtagaattt aagagtttaa tggattatgc
     8521 aatgaagttt aaatttgatg attggttccc gcttttatgt gataactcgg cgtatgttat
     8581 tcaagaatat ataaaatcaa atcggtataa atctgaccga tagattttga atttaggtgt
     8641 cacaagacac tcttttttcg caccagcgaa aactggttta agccgagtgc gcaaaagaca
     8701 taatcgggaa ttc
//
