LOCUS       62056_8130       11686 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     CDS             669..4769
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     promoter        4827..5337
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             5349..5744
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5745..5798
                     /codon_start=1
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5799..6542
                     /codon_start=1
                     /label=rtTA-Advanced
                     /note="improved tetracycline-controlled transactivator"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7003..7014)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     LTR             7219..7452
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7524..7658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7685..7820
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7821..7891
                     /label=AmpR promoter
     CDS             7892..8749
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8923..9511
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9799..9820
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9835..9865
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        9934..9952
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             10207..10387
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10434..10559
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    11052..11285
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11470..11514
                     /codon_start=1
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat ggactataag gaccacgacg gagactacaa ggatcatgat attgattaca
      601 aagacgatga cgataagatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc
      661 cagcagccga caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg
      721 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg
      781 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag
      841 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga
      901 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc
      961 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct
     1021 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga
     1081 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg
     1141 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca
     1201 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa
     1261 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga
     1321 gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg
     1381 gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg
     1441 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc
     1501 tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg
     1561 ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg
     1621 cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg
     1681 tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct
     1741 acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca
     1801 tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc
     1861 tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc
     1921 tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa
     1981 agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa
     2041 acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg
     2101 aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg
     2161 ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca
     2221 ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct
     2281 tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag
     2341 tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg
     2401 aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga
     2461 aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata
     2521 tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct
     2581 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct
     2641 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa
     2701 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg
     2761 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata
     2821 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc
     2881 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca
     2941 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg
     3001 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac
     3061 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg
     3121 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg
     3181 accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca
     3241 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga
     3301 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca
     3361 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga
     3421 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga
     3481 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga
     3541 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca
     3601 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca
     3661 aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc
     3721 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct
     3781 acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga
     3841 agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg
     3901 attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg
     3961 aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc
     4021 tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg
     4081 tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga
     4141 gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca
     4201 tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc
     4261 ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg
     4321 aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg
     4381 ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg
     4441 tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga
     4501 gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg
     4561 ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg
     4621 gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca
     4681 ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac
     4741 ggatcgacct gtctcagctg ggaggcgaca aaaggccggc ggccacgaaa aaggccggcc
     4801 aggcaaaaaa gaaaaagtaa ggatccgggg ttggggttgc gccttttcca aggcagccct
     4861 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
     4921 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
     4981 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
     5041 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
     5101 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
     5161 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
     5221 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
     5281 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
     5341 gtaccaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     5401 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     5461 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     5521 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     5581 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     5641 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     5701 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggcgaaggt agaggttctc
     5761 tcctcacttg tggtgatgtt gaagaaaacc ctggtccaat gtctagactg gacaagagca
     5821 aagtcataaa cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa
     5881 ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca
     5941 agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct
     6001 gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct
     6061 gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga
     6121 aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg
     6181 agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg
     6241 aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc
     6301 cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt
     6361 tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc
     6421 cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact
     6481 ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg
     6541 ggtaactaag taaggatcga tccaagatat cgtattctta actatgttgc tccttttacg
     6601 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6661 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6721 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6781 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6841 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6901 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     6961 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7021 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7081 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtttcgcc tcggcgtccg
     7141 gactagaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7201 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     7261 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7321 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7381 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7441 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     7501 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     7561 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7621 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     7681 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7741 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7801 aggaggcttt tttggaggcc tttcaaatat gtatccgctc atgagacaat aaccctgata
     7861 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7921 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7981 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8041 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8101 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8161 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8221 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8281 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8341 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8401 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8461 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8521 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8581 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8641 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8701 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8761 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8821 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8881 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8941 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9001 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9061 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9121 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9181 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9241 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9301 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9361 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9421 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9481 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9541 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9601 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9661 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9721 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     9781 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     9841 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9901 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     9961 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
    10021 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
    10081 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
    10141 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
    10201 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    10261 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    10321 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10381 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
    10441 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
    10501 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
    10561 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
    10621 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
    10681 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
    10741 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
    10801 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
    10861 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
    10921 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
    10981 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
    11041 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
    11101 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
    11161 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
    11221 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
    11281 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
    11341 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
    11401 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
    11461 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
    11521 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
    11581 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
    11641 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggac
//
