LOCUS       40924_13850        5332 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCV3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   VanDrisse CM, Escalante-Semerena JC.
  TITLE     New high-cloning-efficiency vectors for complementation studies and 
            recombinant protein overproduction in Escherichia coli and 
            Salmonella enterica
  JOURNAL   Plasmid (2016) In press
  PUBMED    27234933
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   VanDrisse CM, Escalante-Semerena JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2016) Microbiology, University of Georgia, 120 
            Cedar St., Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 5332)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5332)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 
            (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAR-2016) Microbiology, University of Georgia, 120 Cedar St., 
            Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      240..326
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      418..445
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        464..555
                     /label=AmpR promoter
     rep_origin      1009..1464
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(2022..2678)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2679..2781)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(3307..3852)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(4126..5001)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        5028..5312
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 gctagcgaat tcggaagagc gctcttcgta aaagcttggc tgttttggcg gatgagagaa
       61 gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt
      121 gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg
      181 ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc
      241 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      301 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac
      361 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga
      421 aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa
      481 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      541 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      601 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      661 gatcagttgg ggcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
      721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
      781 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
      841 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
      901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
      961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttac gcgccctgta
     1021 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     1081 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     1141 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     1201 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     1261 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     1321 aaacttgaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
     1381 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     1441 acaaaatatt aacgtttaca atttaaaagg atctaggtga agatcctttt tgataatctc
     1501 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1561 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1621 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1681 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     1741 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1801 ttaccagtca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg
     1861 taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt
     1921 cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca
     1981 ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca
     2041 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca
     2101 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     2161 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     2221 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     2281 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     2341 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     2401 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
     2461 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     2521 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     2581 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     2641 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     2701 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     2761 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg
     2821 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt
     2881 tattcggcgc aaagtgcgtc gggtgatgct gccaacttac tgatttagtg tatgatggtg
     2941 tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga
     3001 cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctagcgga gtgtatactg
     3061 gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa
     3121 aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca
     3181 ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg
     3241 gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa
     3301 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
     3361 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct
     3421 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     3481 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     3541 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
     3601 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
     3661 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
     3721 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
     3781 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
     3841 aaacgatctc aagaagatca tcttattaat cagataaaat atttgctcat gagcccgaag
     3901 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
     3961 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatctgctc atgtttgaca
     4021 gcttatcatc gatgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct
     4081 atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc
     4141 tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca
     4201 ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt
     4261 ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc
     4321 gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg
     4381 cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc
     4441 gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt
     4501 aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga
     4561 atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg
     4621 ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag
     4681 ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc
     4741 ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg
     4801 tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt
     4861 gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc
     4921 ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg
     4981 atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg
     5041 tccatattgc atcagacatt gccgtcactg cgtcttttac tggcwcwtct cgctaaccaa
     5101 accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa
     5161 aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg
     5221 cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg
     5281 ctttttatcg caactctcta ctgtttctcc atacccgttt ttttggagga gg
//
