LOCUS       40924_13840        4517 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCV1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   VanDrisse CM, Escalante-Semerena JC.
  TITLE     New high-cloning-efficiency vectors for complementation studies and 
            recombinant protein overproduction in Escherichia coli and 
            Salmonella enterica
  JOURNAL   Plasmid (2016) In press
  PUBMED    27234933
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   VanDrisse CM, Escalante-Semerena JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2016) Microbiology, University of Georgia, 120 
            Cedar St., Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 4517)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4517)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 
            (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAR-2016) Microbiology, University of Georgia, 120 Cedar St., 
            Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      245..331
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      423..450
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        469..560
                     /label=AmpR promoter
     CDS             561..1418
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1463..1918
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      2029..2617
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3317..4192)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4219..4503
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 gctagcagga ggaattcgga agagcgctct tcgtaaaagc ttggctgttt tggcggatga
       61 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
      121 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
      181 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
      241 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      301 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
      361 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
      421 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
      481 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      541 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      601 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      661 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      721 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      781 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
      841 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      901 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      961 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1021 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1081 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     1141 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     1201 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     1261 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     1321 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1381 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1441 catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     1501 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     1561 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     1621 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     1681 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     1741 gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact caaccctatc
     1801 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     1861 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa
     1921 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1981 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2041 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2101 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2161 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2221 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2281 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2341 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2401 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2461 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2521 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2581 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2641 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2701 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     2761 gaccgagcgc agcgagtcag tgagcgagga agcggawgwg cgcctgatgc ggtattttct
     2821 ccttacgcat ctgtgcggta tttcacaccg catagggtca tggctgcgcc ccgacacccg
     2881 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     2941 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     3001 gcgaggcagc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
     3061 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     3121 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
     3181 tccggcgtag aggatctgct catgtttgac agcttatcat cgatgcataa tgtgcctgtc
     3241 aaatggacga agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct
     3301 gattcgttac caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg
     3361 cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt
     3421 gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa
     3481 gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact
     3541 gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg
     3601 cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc
     3661 gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt
     3721 gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa
     3781 tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc
     3841 ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa
     3901 agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct
     3961 ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt
     4021 tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc
     4081 ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat
     4141 gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca
     4201 tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact
     4261 gcgtctttta ctggcwcwtc tcgctaacca aaccggtaac cccgcttatt aaaagcattc
     4321 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg
     4381 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt
     4441 tatccataag attagcggat cctacctgac gctttttatc gcaactctct actgtttctc
     4501 catacccgtt tttttgg
//
