LOCUS       V011800                 6314 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V011800
VERSION     V011800
KEYWORDS    pCTcon2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD
  TITLE     Isolating and engineering human antibodies using yeast surface
            display.
  JOURNAL   Nat Protoc. 2006;1(2):755-68.
   PUBMED   17406305
REFERENCE   2  (bases 1 to 6314)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6314)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Protoc."; date: "2006"; volume: "1(2)"; pages: "755-68"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..442
                     /label="GAL1 promoter"
                     /note="inducible promoter, regulated by Gal4"
     CDS             489..749
                     /gene="AGA2"
                     /label="A-agglutinin-binding subunit"
                     /note="A-agglutinin-binding subunit from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P32781"
     CDS             768..779
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             780..806
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1059..1088
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     promoter        complement(1422..1440)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1447..1463)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      1604..2059
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(2165..2836)
                     /label="TRP1"
                     /note="phosphoribosylanthranilate isomerase, required for
                     tryptophan biosynthesis"
     promoter        complement(2837..3118)
                     /label="TRP1 promoter"
     primer_bind     complement(3138..3157)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     3257..3279
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3317..3335)
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     misc_feature    complement(3376..3879)
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        3916..4020
                     /label="AmpR promoter"
     CDS             4021..4878
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5052..5640
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     5794..5811
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     protein_bind    5928..5949
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5964..5994
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6002..6018
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6026..6042
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6063..6081
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6111..6314
                     /label="GAL1,10 promoter"
                     /note="divergent inducible promoter, regulated by Gal4"
ORIGIN
        1 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc
       61 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
      121 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
      181 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat
      241 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
      301 aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg
      361 gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc
      421 tctatacttt aacgtcaagg agaaaaaacc ccggatcgaa ttccctactt catacatttt
      481 caattaagat gcagttactt cgctgttttt caatattttc tgttattgct tcagttttag
      541 cacaggaact gacaactata tgcgagcaaa tcccctcacc aactttagaa tcgacgccgt
      601 actctttgtc aacgactact attttggcca acgggaaggc aatgcaagga gtttttgaat
      661 attacaaatc agtaacgttt gtcagtaatt gcggttctca cccctcaaca actagcaaag
      721 gcagccccat aaacacacag tatgttttta aggacaatag ctcgacgatt gaaggtagat
      781 acccatacga cgttccagac tacgctctgc aggctagtgg tggaggaggc tctggtggag
      841 gcggtagcgg aggcggaggg tcggctagct gcggtggcgg cggtactagc aaaatttctc
      901 attttttgaa aatggaatct ttgaatttta ttagagctca tactccatat attaatattt
      961 acaattgtga accagctaat ccatctgaaa aaaattctcc atctactcaa tattgttatt
     1021 ctattcaatc ttcccaggtc gactgcgggg gcggatccga acaaaagctt atttctgaag
     1081 aggacttgta atagctcgag atctgataac aacagtgtag atgtaacaaa atcgactttg
     1141 ttcccactgt acttttagct cgtacaaaat acaatatact tttcatttct ccgtaaacaa
     1201 catgttttcc catgtaatat ccttttctat ttttcgttcc gttaccaact ttacacatac
     1261 tttatatagc tattcacttc tatacactaa aaaactaaga caattttaat tttgctgcct
     1321 gccatatttc aatttgttat aaattcctat aatttatcct attagtagct aaaaaaagat
     1381 gaatgtgaat cgaatcctaa gagaattgag ctccaattcg ccctatagtg agtcgtatta
     1441 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     1501 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     1561 cgatcgcctt tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
     1621 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     1681 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     1741 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     1801 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     1861 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     1921 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     1981 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     2041 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     2101 cacaccgcat agatcggcaa gtgcacaaac aatacttaaa taaatactac tcagtaataa
     2161 cctatttctt agcatttttg acgaaatttg ctattttgtt agagtctttt acaccatttg
     2221 tctccacacc tccgcttaca tcaacaccaa taacgccatt taatctaagc gcatcaccaa
     2281 cattttctgg cgtcagtcca ccagctaaca taaaatgtaa gctttcgggg ctctcttgcc
     2341 ttccaaccca gtcagaaatc gagttccaat ccaaaagttc acctgtccca cctgcttctg
     2401 aatcaaacaa gggaataaac gaatgaggtt tctgtgaagc tgcactgagt agtatgttgc
     2461 agtcttttgg aaatacgagt cttttaataa ctggcaaacc gaggaactct tggtattctt
     2521 gccacgactc atctccatgc agttggacga tatcaatgcc gtaatcattg accagagcca
     2581 aaacatcctc cttaggttga ttacgaaaca cgccaaccaa gtatttcgga gtgcctgaac
     2641 tatttttata tgcttttaca agacttgaaa ttttccttgc aataaccggg tcaattgttc
     2701 tctttctatt gggcacacat ataataccca gcaagtcagc atcggaatct agagcacatt
     2761 ctgcggcctc tgtgctctgc aagccgcaaa ctttcaccaa tggaccagaa ctacctgtga
     2821 aattaataac agacatactc caagctgcct ttgtgtgctt aatcacgtat actcacgtgc
     2881 tcaatagtca ccaatgccct ccctcttggc cctctccttt tcttttttcg accgaattaa
     2941 ttcttaatcg gcaaaaaaag aaaagctccg gatcaagatt gtacgtaagg tgacaagcta
     3001 tttttcaata aagaatatct tccactactg ccatctggcg tcataactgc aaagtacaca
     3061 tatattacga tgctgttcta ttaaatgctt cctatattat atatatagta atgtcgtgat
     3121 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     3181 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     3241 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     3301 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3361 atggtttctt agacggatcg cttgcctgta acttacacgc gcctcgtatc ttttaatgat
     3421 ggaataattt gggaatttac tctgtgttta tttattttta tgttttgtat ttggatttta
     3481 gaaagtaaat aaagaaggta gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt
     3541 taaaaaattt caacaaaaag cgtactttac atatatattt attagacaag aaaagcagat
     3601 taaatagata tacattcgat taacgataag taaaatgtaa aatcacagga ttttcgtgtg
     3661 tggtcttcta cacagacaag atgaaacaat tcggcattaa tacctgagag caggaagagc
     3721 aagataaaag gtagtatttg ttggcgatcc ccctagagtc ttttacatct tcggaaaaca
     3781 aaaactattt tttctttaat ttcttttttt actttctatt tttaatttat atatttatat
     3841 taaaaaattt aaattataat tatttttata gcacgtgatg aaaaggaccc aggtggcact
     3901 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3961 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4021 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4081 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4141 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4201 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4261 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4321 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4381 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4441 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4501 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4561 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4621 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4681 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4741 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4801 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4861 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4921 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4981 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5041 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5101 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5161 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5221 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5281 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5341 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5401 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5461 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5521 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5581 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5641 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5701 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5761 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5821 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     5881 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     5941 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     6001 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     6061 gaaattaacc ctcactaaag ggaacaaaag ctggtaccaa ttccttgaat tttcaaaaat
     6121 tcttactttt tttttggatg gacgcaaaga agtttaataa tcatattaca tggcattacc
     6181 accatataca tatccatatc taatcttact tatatgttgt ggaaatgtaa agagccccat
     6241 tatcttagcc taaaaaaacc ttctctttgg aactttcagt aatacgctta actgctcatt
     6301 gctatattga agta
//
