LOCUS       V014313                 8324 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014313
VERSION     V014313
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             91..4260
                     /gene="MET"
                     /label="Hepatocyte growth factor receptor"
                     /note="Hepatocyte growth factor receptor from Homo sapiens.
                     Accession#: P08581"
     promoter        complement(4350..4368)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    complement(4373..4507)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(4620..4638)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4659..4675)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4683..4699)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4707..4737)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4752..4773)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5061..5649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5823..6680)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6681..6785)
                     /label="AmpR promoter"
     rep_origin      complement(6811..7266)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7340..8324
                     /label="CMV IE94 promoter"
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccgccacc atgaaggccc ccgctgtgct tgcacctggc
      121 atcctcgtgc tcctgtttac cttggtgcag aggagcaatg gggagtgtaa agaggcacta
      181 gcaaagtccg agatgaatgt gaatatgaag tatcagcttc ccaacttcac cgcggaaaca
      241 cccatccaga atgtcattct acatgagcat cacattttcc ttggtgccac taactacatt
      301 tatgttttaa atgaggaaga ccttcagaag gttgctgagt acaagactgg gcctgtgctg
      361 gaacacccag attgtttccc atgtcaggac tgcagcagca aagccaattt atcaggaggt
      421 gtttggaaag ataacatcaa catggctcta gttgtcgaca cctactatga tgatcaactc
      481 attagctgtg gcagcgtcaa cagagggacc tgccagcgac atgtctttcc ccacaatcat
      541 actgctgaca tacagtcgga ggttcactgc atattctccc cacagataga agagcccagc
      601 cagtgtcctg actgtgtggt gagcgccctg ggagccaaag tcctttcatc tgtaaaggac
      661 cggttcatca acttctttgt aggcaatacc ataaattctt cttatttccc agatcatcca
      721 ttgcattcga tatcagtgag aaggctaaag gaaacgaaag atggttttat gtttttgacg
      781 gaccagtcct acattgatgt tttacctgag ttcagagatt cttaccccat taagtatgtc
      841 catgcctttg aaagcaacaa ttttatttac ttcttgacgg tccaaaggga aactctagat
      901 gctcagactt ttcacacaag aataatcagg ttctgttcca taaactctgg attgcattcc
      961 tacatggaaa tgcctctgga gtgtattctc acagaaaaga gaaaaaagag atccacaaag
     1021 aaggaagtgt ttaatatact tcaggctgcg tatgtcagca agcctggggc ccagcttgct
     1081 agacaaatag gagccagcct gaatgatgac attcttttcg gggtgttcgc acaaagcaag
     1141 ccagattctg ccgaaccaat ggatcgatct gccatgtgtg cattccctat caaatatgtc
     1201 aacgacttct tcaacaagat cgtcaacaaa aacaatgtga gatgtctcca gcatttttac
     1261 ggacccaatc atgagcactg ctttaatagg acacttctga gaaattcatc aggctgtgaa
     1321 gcgcgccgtg atgaatatcg aacagagttt accacagctt tgcagcgcgt tgacttattc
     1381 atgggtcaat tcagcgaagt cctcttaaca tctatatcca ccttcattaa aggagacctc
     1441 accatagcta atcttgggac atcagagggt cgcttcatgc aggttgtggt ttctcgatca
     1501 ggaccatcaa cccctcatgt gaattttctc ctggactccc atccagtgtc tccagaagtg
     1561 attgtggagc atacattaaa ccaaaatggc tacacactgg ttatcactgg gaagaagatc
     1621 acgaagatcc cattgaatgg cttgggctgc agacatttcc agtcctgcag tcaatgcctc
     1681 tctgccccac cctttgttca gtgtggctgg tgccacgaca aatgtgtgcg atcggaggaa
     1741 tgcctgagcg ggacatggac tcaacagatc tgtctgcctg caatctacaa ggttttccca
     1801 aatagtgcac cccttgaagg agggacaagg ctgaccatat gtggctggga ctttggattt
     1861 cggaggaata ataaatttga tttaaagaaa actagagttc tccttggaaa tgagagctgc
     1921 accttgactt taagtgagag cacgatgaat acattgaaat gcacagttgg tcctgccatg
     1981 aataagcatt tcaatatgtc cataattatt tcaaatggcc acgggacaac acaatacagt
     2041 acattctcct atgtggatcc tgtaataaca agtatttcgc cgaaatacgg tcctatggct
     2101 ggtggcactt tacttacttt aactggaaat tacctaaaca gtgggaattc tagacacatt
     2161 tcaattggtg gaaaaacatg tactttaaaa agtgtgtcaa acagtattct tgaatgttat
     2221 accccagccc aaaccatttc aactgagttt gctgttaaat tgaaaattga cttagccaac
     2281 cgagagacaa gcatcttcag ttaccgtgaa gatcccattg tctatgaaat tcatccaacc
     2341 aaatctttta ttagtggtgg gagcacaata acaggtgttg ggaaaaacct gaattcagtt
     2401 agtgtcccga gaatggtcat aaatgtgcat gaagcaggaa ggaactttac agtggcatgt
     2461 caacatcgct ctaattcaga gataatctgt tgtaccactc cttccctgca acagctgaat
     2521 ctgcaactcc ccctgaaaac caaagccttt ttcatgttag atgggatcct ttccaaatac
     2581 tttgatctca tttatgtaca taatcctgtg tttaagcctt ttgaaaagcc agtgatgatc
     2641 tcaatgggca atgaaaatgt actggaaatt aagggaaatg atattgaccc tgaagcagtt
     2701 aaaggtgaag tgttaaaagt tggaaataag agctgtgaga atatacactt acattctgaa
     2761 gccgttttat gcacggtccc caatgacctg ctgaaattga acagcgagct aaatatagag
     2821 tggaagcaag caatttcttc aaccgtcctt ggaaaagtaa tagttcaacc agatcagaat
     2881 ttcacaggat tgattgctgg tgttgtctca atatcaacag cactgttatt actacttggg
     2941 tttttcctgt ggctgaaaaa gagaaagcaa attaaagatc tgggcagtga attagttcgc
     3001 tacgatgcaa gagtacacac tcctcatttg gataggcttg taagtgcccg aagtgtaagc
     3061 ccaactacag aaatggtttc aaatgaatct gtagactacc gagctacttt tccagaagat
     3121 cagtttccta attcatctca gaacggttca tgccgacaag tgcagtatcc tctgacagac
     3181 atgtccccca tcctaactag tggggactct gatatatcca gtccattact gcaaaatact
     3241 gtccacattg acctcagtgc tctaaatcca gagctggtcc aggcagtgca gcatgtagtg
     3301 attgggccca gtagcctgat tgtgcatttc aatgaagtca taggaagagg gcattttggt
     3361 tgtgtatatc atgggacttt gttggacaat gatggcaaga aaattcactg tgctgtgaaa
     3421 tccttgaaca gaatcactga cataggagaa gtttcccaat ttctgaccga gggaatcatc
     3481 atgaaagatt ttagtcatcc caatgtcctc tcgctcctgg gaatctgcct gcgaagtgaa
     3541 gggtctccgc tggtggtcct accatacatg aaacatggag atcttcgaaa tttcattcga
     3601 aatgagactc ataatccaac tgtaaaagat cttattggct ttggtcttca agtagccaaa
     3661 ggcatgaaat atcttgcaag caaaaagttt gtccacagag acttggctgc aagaaactgt
     3721 atgctggatg aaaaattcac agtcaaggtt gctgattttg gtcttgccag agacatgtat
     3781 gataaagaat actatagtgt acacaacaaa acaggtgcaa agctgccagt gaagtggatg
     3841 gctttggaaa gtctgcaaac tcaaaagttt accaccaagt cagatgtgtg gtcctttggc
     3901 gtgctcctct gggagctgat gacaagagga gccccacctt atcctgacgt aaacaccttt
     3961 gatataactg tttacttgtt gcaagggaga agactcctac aacccgaata ctgcccagac
     4021 cccttatatg aagtaatgct aaaatgctgg caccctaaag ccgaaatgcg cccatccttt
     4081 tctgaactgg tgtcccggat atcagcgatc ttctctactt tcattgggga gcactatgtc
     4141 catgtgaacg ctacttatgt gaacgtaaaa tgtgtcgctc cgtatccttc tctgttgtca
     4201 tcagaagata acgctgatga tgaggtggac acacgaccag cctccttctg ggagacatca
     4261 tagtgctagt actatgtcaa agcaacagtc cacactttgt ccagaattca ggcctctcga
     4321 gagatcttca tgactagata gctagcctcc ctatagtgag tcgtattacg tagatccaga
     4381 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     4441 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     4501 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     4561 ggttttttaa ttcgcggccg cggcgccaat gcattgggcc cggtacccag cttttgttcc
     4621 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     4681 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     4741 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     4801 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     4861 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     4921 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     4981 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5041 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5101 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5161 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5221 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     5281 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5341 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     5401 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     5461 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     5521 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     5581 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5641 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5701 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     5761 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5821 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     5881 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     5941 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6001 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6061 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6121 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6181 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6241 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     6301 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     6361 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     6421 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     6481 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     6541 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     6601 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     6661 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     6721 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     6781 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     6841 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     6901 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     6961 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     7021 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     7081 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     7141 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     7201 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     7261 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     7321 cttcgctatt acgccagtcg accatagcca attcaatatg gcgtatatgg actcatgcca
     7381 attcaatatg gtggatctgg acctgtgcca attcaatatg gcgtatatgg actcgtgcca
     7441 attcaatatg gtggatctgg accccagcca attcaatatg gcggacttgg caccatgcca
     7501 attcaatatg gcggacttgg cactgtgcca actggggagg ggtctacttg gcacggtgcc
     7561 aagtttgagg aggggtcttg gccctgtgcc aagtccgcca tattgaattg gcatggtgcc
     7621 aataatggcg gccatattgg ctatatgcca ggatcaatat ataggcaata tccaatatgg
     7681 ccctatgcca atatggctat tggccaggtt caatactatg tattggccct atgccatata
     7741 gtattccata tatgggtttt cctattgacg tagatagccc ctcccaatgg gcggtcccat
     7801 ataccatata tggggcttcc taataccgcc catagccact cccccattga cgtcaatggt
     7861 ctctatatat ggtctttcct attgacgtca tatgggcggt cctattgacg tatatggcgc
     7921 ctcccccatt gacgtcaatt acggtaaatg gcccgcctgg ctcaatgccc attgacgtca
     7981 ataggaccac ccaccattga cgtcaatggg atggctcatt gcccattcat atccgttctc
     8041 acgcccccta ttgacgtcaa tgacggtaaa tggcccactt ggcagtacat caatatctat
     8101 taatagtaac ttggcaagta cattactatt ggaaggacgc cagggtacat tggcagtact
     8161 cccattgacg tcaatggcgg taaatggccc gcgatggctg ccaagtacat ccccattgac
     8221 gtcaatgggg aggggcaatg acgcaaatgg gcgttccatt gacgtaaatg ggcggtaggc
     8281 gtgcctaatg ggaggtctat ataagcaatg ctcgtttagg gaac
//
