LOCUS       62056_8025        4157 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        52..1036
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
     promoter        1071..1089
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             1136..1201
                     /codon_start=1
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    complement(1242..1376)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(1489..1507)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1528..1544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1552..1568)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1576..1606)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1621..1642)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1930..2518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2692..3549)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3550..3654)
                     /label=AmpR promoter
     rep_origin      complement(3680..4135)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt cgaccatagc
       61 caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc
      121 caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc
      181 caattcaata tggcggactt ggcaccatgc caattcaata tggcggactt ggcactgtgc
      241 caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg
      301 ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc
      361 caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct attggccagg
      421 ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga
      481 cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg
      541 cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt
      601 catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa
      661 tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg
      721 ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta
      781 aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag tacattacta
      841 ttggaaggac gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc
      901 ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat
      961 gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa
     1021 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
     1081 cactatagaa tacaagctac ttgttctttt tgcaggatcc catcgattcg aattcgacta
     1141 caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa
     1201 gtgactcgag cctctagaac tatagtgagt cgtattacgt agatccagac atgataagat
     1261 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     1321 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     1381 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaat
     1441 tcgcggccgc ggcgccaatg cattgggccc ggtacccagc ttttgttccc tttagtgagg
     1501 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     1561 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1621 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1681 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1741 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1801 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1861 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1921 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1981 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2041 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2101 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2161 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2221 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2281 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2341 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     2401 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2461 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2521 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2581 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2641 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2701 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2761 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2821 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2881 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2941 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3001 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3061 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3121 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3181 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3241 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3301 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3361 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3421 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3481 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3541 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3601 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3661 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     3721 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     3781 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     3841 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     3901 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     3961 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     4021 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     4081 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     4141 tcgccattca ggctgcg
//
