LOCUS       Exported               11261 bp DNA     circular SYN 21-JUL-2025
DEFINITION  Exported.
ACCESSION   V008167
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Tong Y, Charusanti P, Zhang L, Weber T, Lee SY.
  TITLE     CRISPR-Cas9 based engineering of actinomycetal genomes
  JOURNAL   ACS Synth Biol (2015) In press
  PUBMED    25806970
REFERENCE   2  (bases 1 to 11261)
  AUTHORS   Tong Y, Charusanti P, Zhang L, Weber T, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2015) The Novo Nordisk Foundation Center for 
            Biosustainability, Kogle Alle 6, Horsholm 2970, Denmark
REFERENCE   3  (bases 1 to 11261)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11261)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11261)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2015) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-MAR-2015) The Novo Nordisk Foundation Center for 
            Biosustainability, Kogle Alle 6, Horsholm 2970, Denmark"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
            SGRef: number: 1; type: ""Journal Article""; journalName: ""ACS 
            Synth Biol (2015) In press""
            SGRef: number: 2; type: ""Journal Article""; journalName: 
            ""Submitted (23-MAR-2015) The Novo Nordisk Foundation Center for 
            Biosustainability, Kogle Alle 6, Horsholm 2970, Denmark""
            SGRef: number: 3; type: ""Journal Article""
FEATURES             Location/Qualifiers
     source          1..11261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(466..575)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             891..1691
                     /codon_start=1
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDSFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELLAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      1993..2580
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2868..2889
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2904..2934
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2942..2958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2966..2982
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        3120..3195
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      complement(3365..3399)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             3567..7670
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGY"
     terminator      complement(7860..7908)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             8251..9057
                     /gene="tsnR"
                     /label=23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     CDS             9623..11062
                     /codon_start=1
                     /label=pSG5 Rep
                     /note="replication initiator protein from the Streptomyces 
                     ghanaensis plasmid pSG5"
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
ORIGIN
        1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
       61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
      121 cgaagcggta cgcgacccgt gccgggccgc agcggatcgc ggcagcagga gctccccgcc
      181 cgccacggct ggctggatgg tcgcctcacg cgcccgcagg ggtgacggcg tgggtgcggc
      241 ggtcagcccc gtgatcgggc gtcagacggc cgctgaggga ccgcgaccac ccctcccttc
      301 ccgcctgccg tctttcaccg cccggccggt ccgcagacgc cgtacggcca ggccacggag
      361 gattacgggc agatggcgcc cacccggcac ccgggctggg gcaccctgga cagcacgaag
      421 gtccgcccca ctcaccgggg cgggccttcg cgttgagcta gagttcccgc cagcctcgca
      481 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
      541 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
      601 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
      661 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaat gcagctcacg
      721 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
      781 atgccggcct ttgaatgggt tcatgtgcag ctccataagc aaaaggggat gataagttta
      841 tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg atgtcatcag
      901 cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc
      961 tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc
     1021 ggcccctcga agatgggcca cttggactga tcgaggccct gcgtgctgcg ctgggtccgg
     1081 gagggacgct cgtcatgccc tcgtggtcag gtctggacga cgagccgttc gatcctgcca
     1141 cgtcgcccgt tacaccggac cttggagttg tctctgactc attctggcgc ctgccaaatg
     1201 taaagcgcag cgcccatcca tttgcctttg cggcagcggg gccacaggca gagcagatca
     1261 tctctgatcc attgcccctg ccacctcact cgcctgcaag cccggtcgcc cgtgtccatg
     1321 aactcgatgg gcaggtactt ctcctcggcg tgggacacga tgccaacacg acgctgcatc
     1381 ttgccgagtt gttggcaaag gttccctatg gggtgccgag acactgcacc attcttcagg
     1441 atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag cgctttgcct
     1501 tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc ggtcatgcct
     1561 ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa ctgggccgag
     1621 atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat gccgctcgcc
     1681 agtcgattgg ctgagctcat aagttcctat tccgaagttc ctattcgaaa tgaccgacca
     1741 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     1801 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     1861 gctggagttc ttcgcccacc ccaaaaggat ctaggtgaag atcctttttg ataatctcat
     1921 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1981 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2041 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2101 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2161 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2221 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2281 gttaccggat aaggcgcagc ggtcgggctg aacggggggt cgtgcacaca gcccagcttg
     2341 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2401 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2461 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2521 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2581 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2641 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     2701 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     2761 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     2821 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     2881 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     2941 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaattgtacg
     3001 cggtcgatct tgacggctgg cgagaggtgc ggggaggatc tgaccgacgc ggtccacacg
     3061 tggcaccgcg atgctgttgt gggcacaatc gtgccggttg gtaggatcga cggccatggg
     3121 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     3181 gcaccgagtc ggtgcttttt ttacgtaggc gtcagctacg atgttccggg gactgctgat
     3241 ccggtcagca ggtggaagag ggactggatt ccaaagttct caatgctgct tgctgttctt
     3301 gaatgggggg tcgttgacga cgacatggct cgattggcgc gacaagttgc tgcgattctc
     3361 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     3421 ggtcattact ggaccggatc ggggatctgg gctgagggag ccgacggcac gcggcggctc
     3481 acggcgtggc acgcggaacg tccgggcttg cacctcacgt cacgtgagga ggcagcgtgg
     3541 acggcgtcag agaagggagc ggacatatgg acaagaagta ctccatcggc ctcgacatcg
     3601 gcaccaactc cgtgggctgg gcggtcatca ccgacgagta caaggtcccc tccaagaagt
     3661 tcaaggtcct gggcaacacc gaccggcact cgatcaagaa gaacctgatc ggcgccctgc
     3721 tcttcgacag cggcgagacc gccgaggcga cccgcctgaa gcggaccgcg cgtcgccgct
     3781 acacccggcg caagaaccgc atctgctacc tgcaggaaat cttctccaac gagatggcca
     3841 aggtggacga ctcgttcttc caccgcctgg aggagagctt cctggtggag gaggacaaga
     3901 agcacgagcg ccacccgatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt
     3961 accccaccat ctaccacctc cgcaagaagc tggtggactc gaccgacaag gcggacctgc
     4021 ggctcatcta cctggccctc gcgcacatga tcaagttccg cggccacttc ctcatcgagg
     4081 gcgacctgaa cccggacaac tccgacgtgg acaagctctt catccagctg gtgcagacct
     4141 acaaccagct gttcgaggag aaccccatca acgccagcgg cgtggacgcc aaggcgatcc
     4201 tctccgcgcg cctgagcaag tcccggcgcc tggagaacct catcgcccag ctgccgggcg
     4261 agaagaagaa cggcctcttc ggcaacctga tcgcgctgtc gctcggcctg acccccaact
     4321 tcaagagcaa cttcgacctg gccgaggacg cgaagctcca gctgtccaag gacacctacg
     4381 acgacgacct ggacaacctg ctcgcccaga tcggcgacca gtacgcggac ctcttcctgg
     4441 ccgcgaagaa cctctcggac gccatcctgc tcagcgacat cctgcgggtc aacaccgaga
     4501 tcaccaaggc cccgctgtcg gcgagcatga tcaagcggta cgacgagcac caccaggacc
     4561 tgaccctgct caaggccctc gtgcgccagc agctgcccga gaagtacaag gaaatcttct
     4621 tcgaccagtc caagaacggc tacgccggct acatcgacgg cggcgcgtcg caggaggagt
     4681 tctacaagtt catcaagccg atcctggaga agatggacgg caccgaggag ctgctcgtca
     4741 agctgaaccg cgaggacctg ctccgcaagc agcggacctt cgacaacggc tccatcccgc
     4801 accagatcca cctgggcgag ctccacgcca tcctccggcg ccaggaggac ttctacccct
     4861 tcctgaagga caaccgcgag aagatcgaga agatcctgac cttccgcatc ccgtactacg
     4921 tcggccccct ggcccgcggc aactcccggt tcgcgtggat gacccggaag tcggaggaga
     4981 ccatcacccc gtggaacttc gaggaggtcg tggacaaggg cgcgtccgcg cagtcgttca
     5041 tcgagcgcat gaccaacttc gacaagaacc tcccgaacga gaaggtcctg cccaagcact
     5101 ccctgctcta cgagtacttc accgtgtaca acgagctgac caaggtcaag tacgtgaccg
     5161 agggcatgcg gaagccggcc ttcctgtcgg gcgagcagaa gaaggcgatc gtggacctgc
     5221 tcttcaagac caaccgcaag gtcaccgtga agcagctgaa ggaggactac ttcaagaaga
     5281 tcgagtgctt cgactccgtc gagatcagcg gcgtggagga ccgcttcaac gcctccctgg
     5341 gcacctacca cgacctgctc aagatcatca aggacaagga cttcctcgac aacgaggaga
     5401 acgaggacat cctggaggac atcgtcctca ccctgaccct cttcgaggac cgcgagatga
     5461 tcgaggagcg gctcaagacc tacgcccacc tgttcgacga caaggtgatg aagcagctga
     5521 agcgtcgccg ctacaccggc tggggccgcc tctcccggaa gctgatcaac ggcatccggg
     5581 acaagcagag cggcaagacc atcctggact tcctcaagtc cgacggcttc gccaaccgca
     5641 acttcatgca gctcatccac gacgacagcc tgaccttcaa ggaggacatc cagaaggccc
     5701 aggtctcggg ccagggcgac agcctccacg agcacatcgc caacctggcg ggctccccgg
     5761 cgatcaagaa gggcatcctc cagaccgtca aggtcgtgga cgagctggtc aaggtgatgg
     5821 gccgccacaa gcccgagaac atcgtgatcg agatggcccg ggagaaccag accacccaga
     5881 agggccagaa gaactcgcgc gagcggatga agcggatcga ggagggcatc aaggagctcg
     5941 gcagccagat cctgaaggag cacccggtcg agaacaccca gctgcagaac gagaagctgt
     6001 acctctacta cctgcagaac ggccgcgaca tgtacgtgga ccaggagctc gacatcaacc
     6061 ggctgtccga ctacgacgtg gaccacatcg tgccgcagtc cttcctgaag gacgactcga
     6121 tcgacaacaa ggtcctgacc cgctcggaca agaaccgggg caagtccgac aacgtgccct
     6181 cggaggaggt cgtgaagaag atgaagaact actggcgcca gctgctcaac gccaagctca
     6241 tcacccagcg caagttcgac aacctgacca aggccgagcg gggcggcctg agcgagctcg
     6301 acaaggcggg cttcatcaag cgccagctgg tcgagacccg gcagatcacc aagcacgtgg
     6361 cccagatcct ggactcccgg atgaacacca agtacgacga gaacgacaag ctgatccgcg
     6421 aggtcaaggt gatcaccctc aagagcaagc tggtctccga cttccgcaag gacttccagt
     6481 tctacaaggt ccgggagatc aacaactacc accacgccca cgacgcgtac ctgaacgccg
     6541 tcgtgggcac cgcgctgatc aagaagtacc cgaagctgga gtccgagttc gtctacggcg
     6601 actacaaggt ctacgacgtg cgcaagatga tcgccaagag cgagcaggag atcggcaagg
     6661 ccaccgcgaa gtacttcttc tactccaaca tcatgaactt cttcaagacc gagatcaccc
     6721 tggccaacgg cgagatccgc aagcggcccc tgatcgagac caacggcgag accggcgaga
     6781 tcgtctggga caagggccgc gacttcgcca ccgtccggaa ggtgctgtcg atgccgcagg
     6841 tcaacatcgt gaagaagacc gaggtgcaga ccggcggctt cagcaaggag tccatcctcc
     6901 ccaagcgcaa cagcgacaag ctgatcgccc ggaagaagga ctgggacccg aagaagtacg
     6961 gcggcttcga cagccccacc gtcgcctact ccgtgctggt cgtggcgaag gtcgagaagg
     7021 gcaagagcaa gaagctgaag tccgtgaagg agctgctcgg catcaccatc atggagcgct
     7081 cctcgttcga gaagaacccg atcgacttcc tggaggccaa gggctacaag gaggtcaaga
     7141 aggacctcat catcaagctg cccaagtaca gcctgttcga gctggagaac ggccgcaagc
     7201 ggatgctcgc ctccgcgggc gagctgcaga agggcaacga gctggccctc ccgtcgaagt
     7261 acgtcaactt cctgtacctc gcgtcccact acgagaagct gaagggctcg cccgaggaca
     7321 acgagcagaa gcagctcttc gtggagcagc acaagcacta cctggacgag atcatcgagc
     7381 agatcagcga gttcagcaag cgcgtcatcc tggccgacgc gaacctcgac aaggtgctgt
     7441 ccgcctacaa caagcaccgc gacaagccga tccgggagca ggcggagaac atcatccacc
     7501 tgttcaccct caccaacctg ggcgcccccg ccgcgttcaa gtacttcgac accaccatcg
     7561 accgcaagcg gtacacctcc accaaggagg tcctcgacgc gaccctgatc caccagagca
     7621 tcaccggcct gtacgagacc cgcatcgacc tgtcccagct cggcggctac tgaaagcttt
     7681 ctagaggatc cccgggtacc gagctcgaat tccccagatc taaagttttg tcgtctttcc
     7741 agacgttagt aaatgaattt tctgtatgag gttttgctaa acaactttca acagtttcag
     7801 cggagtgaga atagaaagga acaactaaag gaattgcgaa taataatttt ttcacgttga
     7861 aaatctccaa aaaaaaaggc tccaaaagga gcctttaatt gtatcggttt atcagcttgc
     7921 tttcgaggtg aatttcttaa acagcttgat accgatagtt gcgccgacaa tgacaacaac
     7981 catcgcccac gcataaccga tatattcggt cgctgaggct tgcagggagt caaaggccgc
     8041 ttttgcggga tctcgtcgaa ggcactagag gcctatatgc ggggatcgac cgcgcgggtc
     8101 ccggacgggg aagagcgggg agctttgcca gagagcgacg acttcccctt gcgttggtga
     8161 ttgccggtca gggcagccat ccgccatcgt cgcgtagggt gtcacacccc aggaatcgcg
     8221 tcactgaaca cagcagccgg taggacgacc atgactgagt tggacaccat cgcaaatccg
     8281 tccgatcccg cggtgcagcg gatcatcgat gtcaccaagc cgtcgcgatc caacataaag
     8341 acaacgttga tcgaggacgt cgagcccctc atgcacagca tcgcggccgg ggtggagttc
     8401 atcgaggtct acggcagcga cagcagtcct tttccatctg agttgctgga tctgtgcggg
     8461 cggcagaaca taccggtccg cctcatcgac tcctcgatcg tcaaccagtt gttcaagggg
     8521 gagcggaagg ccaagacatt cggcatcgcc cgcgtccctc gcccggccag gttcggcgat
     8581 atcgcgagcc ggcgtgggga cgtcgtcgtt ctcgacgggg tgaagatcgt cgggaacatc
     8641 ggcgcgatag tacgcacgtc gctcgcgctc ggagcgtcgg ggatcatcct ggtcgacagt
     8701 gacatcacca gcatcgcgga ccggcgtctc caaagggcca gccgaggtta cgtcttctcc
     8761 cttcccgtcg ttctctccgg tcgcgaggag gccatcgcct tcattcggga cagcggtatg
     8821 cagctgatga cgctcaaggc ggatggcgac atttccgtga aggaactcgg ggacaatccg
     8881 gatcggctgg ccttgctgtt cggcagcgaa aagggtgggc cttccgacct gttcgaggag
     8941 gcgtcttccg cctcggtttc catccccatg atgagccaga ccgagtctct caacgtttcc
     9001 gtttccctcg gaatcgcgct gcacgagagg atcgacagga atctcgcggc caaccgataa
     9061 gcgcctctgt tcctcggacg ctcggttgct cgacctcgat tcgtcagtga tgatcaacag
     9121 gacccagaaa tggcacgagc ccgggattgg cctcccgggc tcgccgcccc cgaaccggcg
     9181 cagcatgccg agtgaggacg tttgctctgt gacgagcgta cgagagaaca gcgcgtcgac
     9241 accaccgcgc cccgccggac cgaagtgcca ctgcggcacc ccgatcgtcc acatccccgg
     9301 gaagcgcccc aaaatctact gctcggacgc ctgccgaaag cgcgcaaagc gtctcattgc
     9361 ggaccagtca cgcagcgtag caagcacgac tacagcgggt aatccggacg cgggctttag
     9421 tagtaaggga aaatctccgt ccactgcagg tcagagcgct gtaactggaa cgggttctac
     9481 cccaaacgag ggtgccgcga cagccgggcg ggaggacccg cgcgacgagc ggttcgcccg
     9541 gcgggaccgg caccagacgg tgtcgctgaa cgaggcgttc aagggctgcg ggacccggct
     9601 cactgccggg acggccgagc tgatgtggaa gccgggcgag gcgacgtggg gcaacacgtg
     9661 ccgctgcaac aacgtccaca cctgcccgtg gtgcatgagc cgcatcctgg ccgtacgggg
     9721 ctcgaacgtg cagctcgccg ccgacggtct cgcggacgcc gggtacgggc tgcaccttgg
     9781 cacgaacacg ttgcgccact tcgagcgcat ggcgttcggc accgtccgca agggaatgcg
     9841 gcacggcctg gtcgcggtct tgcacgacgg ctggaagggc gcgtacggct cgtccggccg
     9901 ccgctggcgc acgatgcgcg acgacttcgg gatcatcggc tacgagcggg ccttcgagga
     9961 caccttcggc tggggctccg gctggcacct gcactggcac acgctctggg tcacccgcga
    10021 ggtgctcggc ccggacgccc aggccgcgtt ccgcgacgca ctcgccggag cctgggccgc
    10081 cggggtcgaa tccgcgggcg gctacaccgt cagcgagacg tgcgaccggc ccggttgctc
    10141 ctgtgagggc aagggccacg gcaccgacgt gcgcccactc aacggcgcgg acgccgctga
    10201 cggcgatgcg ggcaagcagg cccgctacct ctacaaggac ggcgacaaga ccaagggcgg
    10261 cgtcgccaag atcggcctgg agctggccgg acagaacttc aaggccggtc gcggcgacga
    10321 ccgcatggga ccgctcgacc tgggcgacgc ggcggccgcc gagctgcagc ggcttcgtcg
    10381 gcccggcccg ttcgtcgaga agtaccgcga gcgcgagttc ggcgtcttcc aggtccgcaa
    10441 gcactaccgg tcgcagaacc tgaaccggct gatcaaggaa ctcggcatcc agcaggacgt
    10501 ccgcaccgag gaggagatca ccgacgacac cgagggcctg gtcgccatcg cggtcatccc
    10561 ggcctacatc tggtaccgat acatcgcccg cgtcgccgga cgtcgcctcg acctcatcaa
    10621 ggtcgccgag acgtacggcc taccgggcgt ccggcggctc gtcgagtcct ggggcctggt
    10681 gtggggcaag gacgtcctgg acccgcccgc cccggaagcg ccggccgcgc cgggcgacct
    10741 tgacgctgac cagatgaggt tcgaggtcat gtccgaggag gaagctgctt tccgcgaggc
    10801 gcgccggaag gccaacgagg cccgcacgga ggagctggcc gcttcgctcg accgagtgcg
    10861 tcagccgaag aaggaggcga tccgcccgac gatcagcctt cggaagcgcc tgaagccgaa
    10921 gccggtcacc gtcgacgtca agactccccc gcccggcgcc gcgtcgcccg tgtgccgccg
    10981 ctgcaagggc aagctcgccc cggtactcca gccctggggc cagcaccccg gagactgcct
    11041 ccgcgtcgac acagcggtcg cgtagggccg ctgagggacc gccatgagac cgccaccccg
    11101 gacatccggg tggcggtctc atggcggtcc cggcgcgaaa gccgagggtc tgcctgccgt
    11161 gaggtggccg gcggacctgg gttactgctg gaatcccgcg cccagtccga gcgtccgccg
    11221 gcgcttttca cctggctccg ggtgctcgtc gatgagcacc a
//
