LOCUS       40924_13405        7032 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCRII-tra4R-3xP3CFP-tra4L, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Karaminejadranjbar M, Eckermann KN, Ahmed HMM., Dippel S, Wimmer EA.
  TITLE     Instant induction of resistance evolution in CRISPR/Cas9 gene drive
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Karaminejadranjbar M, Eckermann KN, Ahmed HMM., Dippel S, Wimmer EA.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-NOV-2016) Developmental Biology, 
            Georg-August-University Goettingen, Justus-von-Liebig-Weg 11, 
            Goettingen 37077, Germany
REFERENCE   3  (bases 1 to 7032)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7032)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-NOV-2016) Developmental Biology, Georg-August-University 
            Goettingen, Justus-von-Liebig-Weg 11, Goettingen 37077, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Geneious v. 8.0.5
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..1033
                     /label=left homologous arm tra g4
                     /note="left homologous arm tra g4"
     protein_bind    1034..1083
                     /label=attP binding site
                     /bound_moiety="attP"
     polyA_signal    complement(1117..1198)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1324..2040)
                     /label=ECFP
                     /note="enhanced CFP"
     regulatory      complement(2194..2282)
                     /label=3xP3
                     /note="3xP3"
                     /regulatory_class="promoter"
     protein_bind    complement(2288..2337)
                     /label=attP binding site
                     /bound_moiety="attP"
     promoter        complement(3201..3219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3226..3242)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3383..3811
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             4155..4946
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             4967..5824
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5998..6586
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6874..6895
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6910..6940
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6948..6964
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6972..6988
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7006..7024
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gctatgcatc aagctcagcc gacagcgaga gaaactcaca tcgccgatgc tggacagcat
       61 ttggggcgaa tttccggacg agcagccagt gcgcaacatc ggtcagctga acgaaccgcc
      121 agagtttatt ctggacttgg tcacagtagt gcggattatt gatgttgcgc ttaccctcaa
      181 tcgggacttt gatgaatcgt ctgagagttc cctgatgttc ctgccggagg ccgacgaaac
      241 cgtgctattc cgcaccatat gtgtggccaa ttcgccgacg ccaacgcaga gccatctatt
      301 ggagccagtg gatgagctat tggaggcgga caccacaacg ttgacgcccg tcgagcaaaa
      361 ctggtgtgtg cagccaggtt tgctggaatt cggagtgggc tgttcgccac tggccacttt
      421 gtccgtaacc aacagcttct gctccaggca gctcatcgag gtcacatgca atgtgcccga
      481 tttggtgcga atcagtccca gtgagtgcta cgtatccccg gatggcggcc acgcggagat
      541 cgatgtgcgc ctgctccgct cacccaggca ggatttgctc gaaagggagc cgctcattgt
      601 ggtctccatg gaaaacgagc gcatcagtgt cccgattagt tttaaatttt aaaatttgtt
      661 ttatttgtgc ctgctaattc tgctttccct atgtttgtgt agttagttta aacaaaaatt
      721 gtattgtgta cttgtgaaat gaaaccgtgt ttgcagagta tttgtaagga aaacgcataa
      781 ccctaaagtt aaaatactcg cttgtttcct aaaagttgaa tttagcttaa ataaaaacta
      841 ataaagtata tgagttaatt ttaaattttg atatgttgct ctcgaatatt caaaaagtaa
      901 cgtatatgta gcgatagttc gtcgaaaagt gagttggcgg gcacattgca aggtgcaagc
      961 cgagtacggt cacactgagg aaagtgcatc atttaatttc cagcattttc cgatgaaaat
     1021 ggatgccgac agagtagtgc cccaactggg gtaacctttg agttctctca gttgggggcg
     1081 tagggatgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     1141 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     1201 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     1261 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccgct
     1321 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
     1381 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta
     1441 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
     1501 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc
     1561 gttcttctgc ttgtcggcgg tgatatagac gttgtggctg atgtagttgt actccagctt
     1621 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
     1681 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
     1741 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
     1801 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccc caggtcaggg tggtcacgag
     1861 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
     1921 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
     1981 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
     2041 ggtggcgacc ggtggatcgt accgtcgact ctagcggtac cccgattgtt tagcttgttc
     2101 agctgcgctt gtttatttgc ttagctttcg cttagcgacg tgttcacttt gcttgtttga
     2161 attgaattgt cgctccgtag acgaagcgcc tctatttata ctccggcggt cgagggttcg
     2221 aaatcgataa gcttggatcc taattgaatt agctctaatt gaattagtct ctaatgaata
     2281 atccccccta cgcccccaac tgagagaact caaaggttac tccagttggg gcactactgc
     2341 agtggagccc agcatcgagg tacccactat atagtataac cgatctgatc agattctgat
     2401 ccagtgccat ctttttgttg ttttttttct agtgtcatat tgtgtgaaat gtgaaatgga
     2461 tgagaatgaa cgctggacga ccagacagag aaagagaagc taggacaata ggactctcaa
     2521 ctgcgcatta cgtggattcc gtctccgacg atgcgccaaa cactatgcgt tagatgcatt
     2581 gactaaccgc acactctttt cacatagatt cccgtggctc aaggtctcga tcccggcgag
     2641 aaagagaata ccatgggcga tcaagcgaga gggacagcag aaagaaggag cacaagattc
     2701 cgtactttgc agacgaggtt cgagaacagg atcggttgag aagattgcgc caaagagcgc
     2761 accaatccac cagacgcact cgctccagat ccagatcaca gtcgtccatc agagagagca
     2821 ggcacagaag gcatcgccag cgctctagga gccgcaatcg cagccgaagt cgcagcagtg
     2881 aacgaaaacg ccgtcaacgg agccgaagtc gcagcagtga acgaagacgc cgtcaacgga
     2941 gcccgcatcg gtataatcct ccgccaaaga tcatcaacta ctatgtgcaa gtgccaccac
     3001 aggatttcta cgttagtagt tatttgtatt gaaacaaata acaaatctaa cttaaatcga
     3061 ttttgcaggg gatgtctggt atgcagcaaa gttttggata ccaaaggcta ccacgtcctc
     3121 cgccgtttcc accggccccc tacagatacc gccagcgacc gccgttcatt ggagttccgc
     3181 gatttggcta cacaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
     3241 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3301 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3361 gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3421 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     3481 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     3541 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     3601 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     3661 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     3721 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     3781 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa ttcagggcgc aagggctgct
     3841 aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
     3901 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
     3961 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
     4021 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     4081 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat
     4141 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4201 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4261 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4321 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4381 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4441 aagtgccggg gcaggatctc ctgtcatccc accttgctcc tgccgagaaa gtatccatca
     4501 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4561 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4621 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4681 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4741 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4801 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4861 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4921 tctatcgcct tcttgacgag ttcttctgaa ttgaaaaagg aagagtatga gtattcaaca
     4981 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5041 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5101 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5161 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5221 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5281 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5341 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5401 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5461 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5521 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5581 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5641 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5701 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5761 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5821 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5881 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5941 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6001 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6061 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6121 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6181 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6241 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6301 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6361 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6421 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6481 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6541 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6601 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6661 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6721 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6781 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     6841 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     6901 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     6961 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctattta ggtgacacta
     7021 tagaatactc aa
//
