LOCUS       40924_13380        5688 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pCRE-Luc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Zheng C-F.
  TITLE     pCRE-Luc plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines, San Diego, CA 92037, USA
REFERENCE   3  (bases 1 to 5688)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines, San Diego, CA 92037, USA
REFERENCE   4  (bases 1 to 5688)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-1999) Technical Services, Stratagene, 11011 N. 
            Torrey Pines, San Diego, CA 92037, USA
REFERENCE   5  (bases 1 to 5688)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 5688)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-1998) Technical Services, Stratagene, 11011 N. Torrey Pines,
            San Diego, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (29-OCT-1998) Technical Services, Stratagene, 11011 N. Torrey Pines,
            San Diego, CA 92037, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (26-JAN-1999) Technical Services, Stratagene, 11011 N. Torrey Pines,
            San Diego, CA 92037, USA"
COMMENT     SGRef: number: 5; type: "Journal Article"
COMMENT     On Jan 26, 1999 this sequence version replaced gi:3808229.
FEATURES             Location/Qualifiers
     source          1..5688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..136
                     /label=AmpR promoter
     CDS             137..994
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1168..1755
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1934..2074)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     polyA_signal    2313..2445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2539..2671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2750..2781
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2808..4457
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          4591..4656
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    5226..5360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
     1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
     1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
     2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
     2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttcact gcattctagt
     2401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcagcca tatcacattt
     2461 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     2521 atgaatgcaa ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2581 agcatcacaa atttcacaaa taaagcaatt tttcactgca ttctattgtg gtttgtccaa
     2641 actcatcaat gtatcttatc atgtctggat ccaagctagc cagcctgacg tcagaggcct
     2701 gacgtcagag agcctgacgt cagagagcct gacgtcagag cggagactct agagggtata
     2761 taatggaagc tcgaattcca gcttggcatt ccggtactgt tggtaaaatg gaagacgcca
     2821 aaaacataaa gaaaggcccg gcgccattct atcctctaga ggatggaacc gctggagagc
     2881 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
     2941 cacatatcga ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt cggttggcag
     3001 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
     3061 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
     3121 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gaacatttcg cagcctaccg
     3181 tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa
     3241 taatccagaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
     3301 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtaccagagt
     3361 cctttgatcg tgacaaaaca attgcactga taatgaattc ctctggatct actgggttac
     3421 ctaagggtgt ggcccttccg catagaactg cctgcgtcag attctcgcat gccagagatc
     3481 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
     3541 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
     3601 tgtatagatt tgaagaagag ctgtttttac gatcccttca ggattacaaa attcaaagtg
     3661 cgttgctagt accaacccta ttttcattct tcgccaaaag cactctgatt gacaaatacg
     3721 atttatctaa tttacacgaa attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg
     3781 aagcggttgc aaaacgcttc catcttccag ggatacgaca aggatatggg ctcactgaga
     3841 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     3901 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     3961 atcagagagg cgaattatgt gtcagaggac ctatgattat gtccggttat gtaaacaatc
     4021 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     4081 actgggacga agacgaacac ttcttcatag ttgaccgctt gaagtcttta attaaataca
     4141 aaggatatca ggtggccccc gctgaattgg aatcgatatt gttacaacac cccaacatct
     4201 tcgacgcggg cgtggcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     4261 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     4321 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     4381 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     4441 gcggaaagtc caaattgtaa aatgtaactg tattcagcga tgacgaaatt cttagctatt
     4501 gtaatactct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     4561 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     4621 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aacttatgaa
     4681 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     4741 tctagtgatg atgaggctac tgctgactct caacattcta ctctcaaaag aagagaaagg
     4801 tagagaccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     4861 aatagaactc ttgcttgctt tgctatttac aaccacaaag gaaaaagctg cactgctata
     4921 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
     4981 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
     5041 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
     5101 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
     5161 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     5221 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5281 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5341 tatcttatca tgtctggatc cccaggaagc tcctctgtgt cctcataaac cctaacctcc
     5401 tctacttgag aggacattcc aatcataggc tgcccatcca ccctctgtgt cctcctgtta
     5461 attaggtcac ttaacaaaaa ggaaattggg taggggtttt tcacagaccg ctttctaagg
     5521 ggtaatttta aaatatctgg gaagtccctt ccactgctgt gttccagaag tgttggtaaa
     5581 cagcccacaa atgtcaacag cagaaacata caagctgtca ctttgcacaa agggcctcgt
     5641 gatacgccta tttttatagg ttaatgtcat gataataatg gtttctta
//
