LOCUS pCRE-EGFP 4077 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046791 VERSION V046791 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers CDS 288..1007 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 1047..1168 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(1587..2175) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(2346..3206) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(3207..3311) /gene="bla" /label="AmpR promoter" rep_origin 3338..3793 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" polyA_signal 3924..3972 /note="synthetic polyadenylation signal" /label="poly(A) signal" misc_feature 3986..4077 /note="RNA polymerase II transcriptional pause signal from the human α2 globin gene" /label="pause site" ORIGIN 1 atcgataggt accgagctct tacgcgtgct agcgcaccag acagtgacgt cagctgccag 61 atcccatggc cgtcatactg tgacgtcttt cagacacccc attgacgtca atgggagaac 121 agatctgccg ccccgactgc atctgcgtgt tcgaattcgc caatgacaag acgctgggcg 181 gggtttgtgt catcatagaa ctaaagacat gcaaatatat ttcttccggg gacaccgcca 241 gcaaacgcga gcaacgggcc acggggatga agcagaagct tgccaccatg gtgagcaagg 301 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 361 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 421 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 481 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 541 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 601 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 661 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 721 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 781 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 841 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 901 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 961 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtagtct agagtcgggg 1021 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac 1081 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt 1141 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca 1201 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa 1261 aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc 1321 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac 1381 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 1441 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 1501 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 1561 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 1621 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 1681 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 1741 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 1801 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1861 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1921 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1981 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 2041 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 2101 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 2161 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 2221 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 2281 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 2341 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 2401 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 2461 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 2521 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 2581 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 2641 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 2701 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 2761 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 2821 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 2881 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 2941 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 3001 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 3061 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 3121 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 3181 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 3241 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 3301 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta 3361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 3421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 3481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 3541 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 3601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 3661 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 3721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 3781 acgcttacaa tttgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 3841 cgggcctctt cgctattacg ccagcccaag ctaccatgat aagtaagtaa tattaaggta 3901 cgggaggtac ttggagcggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg 3961 gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa 4021 acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctct //