LOCUS       pCRE-EGFP               4077 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046791
VERSION     V046791
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             288..1007
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    1047..1168
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(1587..2175)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(2346..3206)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(3207..3311)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      3338..3793
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     polyA_signal    3924..3972
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    3986..4077
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 atcgataggt accgagctct tacgcgtgct agcgcaccag acagtgacgt cagctgccag
       61 atcccatggc cgtcatactg tgacgtcttt cagacacccc attgacgtca atgggagaac
      121 agatctgccg ccccgactgc atctgcgtgt tcgaattcgc caatgacaag acgctgggcg
      181 gggtttgtgt catcatagaa ctaaagacat gcaaatatat ttcttccggg gacaccgcca
      241 gcaaacgcga gcaacgggcc acggggatga agcagaagct tgccaccatg gtgagcaagg
      301 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      361 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      421 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      481 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      541 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      601 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      661 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      721 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
      781 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      841 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      901 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      961 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtagtct agagtcgggg
     1021 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     1081 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     1141 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     1201 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     1261 aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     1321 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     1381 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1441 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1501 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1561 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1621 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1681 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1741 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1801 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1861 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1921 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1981 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2041 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2101 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2161 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2221 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2281 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2341 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2401 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2461 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2521 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2581 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2641 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2701 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2761 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2821 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2881 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2941 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3001 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3061 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3121 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3181 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3241 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3301 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     3361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3541 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3661 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     3721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3781 acgcttacaa tttgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     3841 cgggcctctt cgctattacg ccagcccaag ctaccatgat aagtaagtaa tattaaggta
     3901 cgggaggtac ttggagcggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
     3961 gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa
     4021 acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctct
//
