LOCUS       40924_13335        5536 bp DNA     circular SYN 17-DEC-2018
DEFINITION  C.glutamicum-E.coli shuttle vector pCRB12i, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Oide S, Gunji W, Moteki Y, Yamamoto S, Suda M, Jojima T, Yukawa H, 
            Inui M.
  TITLE     Thermal and Solvent Stress Cross-Tolerance Conferred to 
            Corynebacterium glutamicum by Adaptive Laboratory Evolution
  JOURNAL   Appl. Environ. Microbiol. 81 (7), 2284-2298 (2015)
  PUBMED    25595768
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Gunji W, Oide S, Inui M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2015) Microbiology Group, Research Institute of 
            Innovative Technology for the Earth, 9-2 Kizugawadai, Kyoto 
            619-0292, Japan
REFERENCE   3  (bases 1 to 5536)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5536)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2015"; volume: "81"; issue: "7"; pages:
            "2284-2298"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-JAN-2015) Microbiology Group, Research Institute of Innovative 
            Technology for the Earth, 9-2 Kizugawadai, Kyoto 619-0292, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             71..1150
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1166..1187
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      1621..2209
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2757..3569
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    3635..5528
                     /note="derived from Corynebacterium glutamicum plasmid
                     pCG1"
ORIGIN
        1 ttaattaagc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
       61 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag gcaccgggat
     1201 ctgaattcga gctcggtacc cggggatcct ctagagtcga caacacccct tgtattactg
     1261 tttatgtaag cagacagttt tattgttcat gatgatatat ttctatcttg tgcaatgtaa
     1321 catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg
     1381 aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc
     1441 aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc cctcactctc
     1501 tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc
     1561 agacctctcg acggagttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1621 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1681 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1741 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1801 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1861 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1921 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1981 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2041 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2101 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc aggtttcgcc acctctgact
     2161 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2221 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2281 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     2341 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag aagctcgcac
     2401 attcagcagc gtttttcagc gcgttttcga tcaacgtttc aatgttggta tcaacaccag
     2461 gtttaacttt gaacttatcg gcactgacgg ttactgattt tgaacttttg ctttgccacg
     2521 gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc aaaagttcgc
     2581 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgagct ggcacgctcg
     2641 agcaacaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     2701 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     2761 gccatattca acgggaaacg tcttgctcga agccgcgatt aaattccaac atggatgctg
     2821 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     2881 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     2941 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     3001 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     3061 ccgggaaaac agcattccag gtattagaag gatatcctga ttcaggtgaa aatattgttg
     3121 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     3181 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     3241 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     3301 tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     3361 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     3421 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     3481 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     3541 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact
     3601 ggcagagcat tacgctgact tgacgggact cgagtctagc atggtcgtca cagagctgga
     3661 agcggcagcg agaattatcc gcgatcgtgg cgcggtgccc gcaggcatga caaacatcgt
     3721 aaatgccgcg tttcgtgtgg ccgtggccgc ccaggacgtg tcagcgccgc caccacctgc
     3781 accgaatcgg cagcagcgtc gcgcgtcgaa aaagcgcaca ggcggcaaga agcgataagc
     3841 tgcacgaata cctgaaaaat gttgaacgcc ccgtgagcgg taactcacag ggcgtcggct
     3901 aacccccagt ccaaacctgg gagaaagcgc tcaaaaatga ctctagcgga ttcacgagac
     3961 attgacacac cggcctggaa attttccgct gatctgttcg acacccatcc cgagctcgcg
     4021 ctgcgatcac gtggctggac gagcgaagac cgccgcgaat tcctcgctca cctgggcaga
     4081 gaaaatttcc agggcagcaa gacccgcgac ttcgccagcg cttggatcaa agacccggac
     4141 acgggagaaa cacagccgaa gttataccga gttggttcaa aatcgcttgc ccggtgccag
     4201 tatgttgctc tgacgcacgc gcagcacgca gccgtgcttg tcctggacat tgatgtgccg
     4261 agccaccagg ccggcgggaa aatcgagcac gtaaaccccg aggtctacgc gattttggag
     4321 cgctgggcac gcctggaaaa agcgccagct tggatcggcg tgaatccact gagcgggaaa
     4381 tgccagctca tctggctcat tgatccggtg tatgccgcag caggcatgag cagcccgaat
     4441 atgcgcctgc tggctgcaac gaccgaggaa atgacccgcg ttttcggcgc tgaccaggct
     4501 ttttcacata ggctgagccg gtggccactg cacgtctccg acgatcccac cgcgtaccgc
     4561 tggcatgccc agcacaatcg cgtggatcgc ctagctgatc ttatggaggt tgctcgcatg
     4621 atctcaggca cagaaaaacc taaaaaacgc tatgagcagg agttttctag cggacgggca
     4681 cgtatcgaag cggcaagaaa agccactgcg gaagcaaaag cacttgccac gcttgaagca
     4741 agcctgccga gcgccgctga agcgtctgga gagctgatcg acggcgtccg tgtcctctgg
     4801 actgctccag ggcgtgccgc ccgtgatgag acggcttttc gccacgcttt gactgtggga
     4861 taccagttaa aagcggctgg tgagcgccta aaagacacca agatcatcga cgcctacgag
     4921 cgtgcctaca ccgtcgctca ggcggtcgga gcagacggcc gtgagcctga tctgccgccg
     4981 atgcgtgacc gccagacgat ggcgcgacgt gtgcgcggct acgtcgctaa aggccagcca
     5041 gtcgtccctg ctcgtcagac agagacgcag agcagccgag ggcgaaaagc tctggccact
     5101 atgggaagac gtggcggtaa aaaggccgca gaacgctgga aagacccaaa cagtgagtac
     5161 gcccgagcac agcgagaaaa actagctaag tccagtcaac gacaagctag gaaagctaaa
     5221 ggaaatcgct tgaccattgc aggttggttt atgactgttg agggagagac tggctcgtgg
     5281 ccgacaatca atgaagctat gtctgaattt agcgtgtcac gtcagaccgt gaatagagca
     5341 cttaagtctg cgggcattga acttccacga ggacgccgta aagcttccca gtaaatgtgc
     5401 catctcgtag gcagataacg gttccccccg taggggtctc tctcttggcc tcctttctag
     5461 gtcgggctga ttgctcttga agctctctag gggggctcac accataggca gataacggtt
     5521 ccagatctag gtttcc
//
