LOCUS       40924_13310        4972 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cre-lox Univector acceptor vector pCR703, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Jia F, Gampala SS, Mittal A, Luo Q, Rock CD.
  TITLE     Cre-lox univector acceptor vectors for functional screening in 
            protoplasts: analysis of Arabidopsis donor cDNAs encoding ABSCISIC 
            ACID INSENSITIVE1-like protein phosphatases
  JOURNAL   Plant Mol. Biol. 70 (6), 693-708 (2009)
  PUBMED    19499346
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Rock CD, Jia F, Gampala SSL., Mittal A, Luo Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2009) Biological Science, Texas Tech University, 
            2500 Broadway, Lubbock, TX 79409, USA
REFERENCE   3  (bases 1 to 4972)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4972)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2009"; volume: "70"; issue: "6"; pages: "693-708"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2009) Biological Science, Texas Tech University, 2500 
            Broadway, Lubbock, TX 79409, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        411..2402
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2429..2455
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2456..2482
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     protein_bind    complement(2483..2516)
                     /label=loxH
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021). The loxH variant 
                     was optimized for expression constructs but remains 
                     compatible with loxP."
     regulatory      2517..2736
                     /label=Cauliflower mosaic virus (CaMV) 35S terminator
                     /note="Cauliflower mosaic virus (CaMV) 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2751..2767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2775..2791)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2799..2829)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2844..2865)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3153..3741)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3915..4772)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4773..4877)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagtgca
      421 gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa
      481 aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca
      541 tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt
      601 ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga gtattttgac
      661 aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat
      721 agcttcacct atataatact tcatccattt tattagtaca tccatttagg gtttagggtt
      781 aatggttttt atagactaat ttttttagta catctatttt attctatttt agcctctaaa
      841 ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata taaaatagaa
      901 taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa
      961 acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac
     1021 ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca
     1081 tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg
     1141 ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc
     1201 ctcctcctcc tctcacggca cggcagctac gggggattcc tttcccaccg ctccttcgct
     1261 ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct
     1321 cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac
     1381 ctccgcttca aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc
     1441 ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat
     1501 ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag
     1561 acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag
     1621 ccgttccgca gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt
     1681 tgcccttttc ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg
     1741 cttttttttg tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt
     1801 agaattctgt ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca
     1861 tacatattca tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat
     1921 acatgttgat gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt
     1981 gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt
     2041 caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata
     2101 gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg
     2161 ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga
     2221 gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg
     2281 gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt
     2341 tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc
     2401 aggtcgactc tagaggatcc aaatggctta cccttacgat gtgcctgatt acgcttatcc
     2461 ttatgatgtt cctgattatg ctattacctc atatagcata cattatacga agttatgaat
     2521 tcccatgccc gctgaaatca ccagtctctc tctacaaatc tatctctctc tataataatg
     2581 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
     2641 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     2701 taattcctaa aaccaaaatc caggggtacc gagctcaaat tcgtaatcat gtcatagctg
     2761 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     2821 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     2881 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     2941 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     3001 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3061 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3121 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3181 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3241 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3301 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3361 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3421 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3481 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3541 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     3601 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3661 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3721 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3781 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3841 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3901 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3961 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4021 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4081 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4141 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4201 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4261 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4321 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4381 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4441 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4501 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4561 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4621 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4681 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4741 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4801 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4861 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     4921 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
