LOCUS       40924_13305        4937 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cre-lox Univector acceptor vector pCR702, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Jia F, Gampala SS, Mittal A, Luo Q, Rock CD.
  TITLE     Cre-lox univector acceptor vectors for functional screening in 
            protoplasts: analysis of Arabidopsis donor cDNAs encoding ABSCISIC 
            ACID INSENSITIVE1-like protein phosphatases
  JOURNAL   Plant Mol. Biol. 70 (6), 693-708 (2009)
  PUBMED    19499346
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Rock CD, Jia F, Gampala SSL., Mittal A, Luo Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2009) Biological Science, Texas Tech University, 
            2500 Broadway, Lubbock, TX 79409, USA
REFERENCE   3  (bases 1 to 4937)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4937)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2009"; volume: "70"; issue: "6"; pages: "693-708"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2009) Biological Science, Texas Tech University, 2500 
            Broadway, Lubbock, TX 79409, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        411..2402
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2430..2447
                     /label=6xHis
                     /note="6xHis affinity tag"
     protein_bind    complement(2448..2481)
                     /label=loxH
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021). The loxH variant 
                     was optimized for expression constructs but remains 
                     compatible with loxP."
     regulatory      2482..2701
                     /label=Cauliflower mosaic virus (CaMV) 35S terminator
                     /note="Cauliflower mosaic virus (CaMV) 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2716..2732)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2740..2756)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2764..2794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2809..2830)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3118..3706)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3880..4737)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4738..4842)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagtgca
      421 gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa
      481 aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca
      541 tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt
      601 ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga gtattttgac
      661 aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat
      721 agcttcacct atataatact tcatccattt tattagtaca tccatttagg gtttagggtt
      781 aatggttttt atagactaat ttttttagta catctatttt attctatttt agcctctaaa
      841 ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata taaaatagaa
      901 taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa
      961 acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac
     1021 ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca
     1081 tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg
     1141 ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc
     1201 ctcctcctcc tctcacggca cggcagctac gggggattcc tttcccaccg ctccttcgct
     1261 ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct
     1321 cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac
     1381 ctccgcttca aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc
     1441 ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat
     1501 ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag
     1561 acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag
     1621 ccgttccgca gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt
     1681 tgcccttttc ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg
     1741 cttttttttg tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt
     1801 agaattctgt ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca
     1861 tacatattca tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat
     1921 acatgttgat gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt
     1981 gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt
     2041 caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata
     2101 gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg
     2161 ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga
     2221 gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg
     2281 gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt
     2341 tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc
     2401 aggtcgactc tagaggatcc aaaatggctc atcatcatca ccatcatatt acctcatata
     2461 gcatacatta tacgaagtta tgaattccca tgcccgctga aatcaccagt ctctctctac
     2521 aaatctatct ctctctataa taatgtgtga gtagttccca gataagggaa ttagggttct
     2581 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     2641 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccaggg gtaccgagct
     2701 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     2761 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2821 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2881 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2941 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3001 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3061 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3121 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3181 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3241 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3301 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3361 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3421 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3481 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3541 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3601 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3661 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3721 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3781 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3841 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3901 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3961 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4021 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4081 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4141 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4201 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4261 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4321 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4381 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4441 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4501 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4561 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4621 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4681 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4741 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4801 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4861 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     4921 cacgaggccc tttcgtc
//
