LOCUS       40924_13290        6734 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCR3VSV-CED-4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6734)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6734)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             691..723
                     /label=VSV-G tag
                     /note="epitope tag from vesicular stomatitis virus G
                     protein"
     CDS             736..2385
                     /codon_start=1
                     /note="unnamed protein product; CED-4"
                     /protein_id="SJL88336.1"
                     /translation="MLCEIECRALSTAHTRLIHDFEPRDALTYLEGKNIFTEDHSELIS
                     KMSTRLERIANFLRIYRRQASELGPLIDFFNYNNQSHLADFLEDYIDFAINEPDLLRPV
                     VIAPQFSRQMLDRKLLLGNVPKQMTCYIREYHVDRVIKKLDEMCDLDSFFLFLHGRAGS
                     GKSVIASQALSKSDQLIGINYDSIVWLKDSGTAPKSTFDLFTDILLMLKSEDDLLNFPS
                     VEHVTSVVLKRMICNALIDRPNTLFVFDDVVQEETIRWAQELRLRCLVTTRDVEISNAA
                     SQTCEFIEVTSLEIDECYDFLEAYGMPMPVGEKEEDVLNKTIELSSGNPATLMMFFKSC
                     EPKTFEKMAQLNNKLESRGLVGVECITPYSYKSLAMALQRCVEVLSDEDRSALAFAVVM
                     PPGVDIPVKLWSCVIPVDICSNEEEQLDDEVADRLKRLSKRGALLSGKRMPVLTFKIDH
                     IIHMFLKHVVDAQTIANGISILEQRLLEIGNNNVSVPERHIPSHFQKFRRSSASEMYPK
                     TTEETVIRPEDFPKFMQLHQKFYDSLKNFACC"
     promoter        complement(2449..2467)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2493..2717
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2849..3437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    complement(3766..3813)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4048..4833)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4868..5225)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             complement(5288..6145)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6146..6250)
                     /label=AmpR promoter
     rep_origin      6277..6732
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccggatc cgccaccatg tacaccgaca tcgagatgaa ccggttgggc
      721 aaggaattgg gtcagatgct ctgcgaaatc gaatgccgcg ctttgagcac ggcacacacg
      781 aggctcatcc acgactttga accacgtgac gcattgactt atttagaagg caaaaacatt
      841 ttcacagaag atcattctga acttatcagt aaaatgtcaa ctcgcctcga gaggatcgcc
      901 aattttcttc gaatctatcg acgtcaagct tctgaacttg gaccactcat cgactttttc
      961 aactacaaca atcaaagtca ccttgctgat ttcctcgaag actacatcga ttttgcgata
     1021 aatgagccag atctacttcg tccagtagtg attgctccac aattttcccg acaaatgctc
     1081 gataggaaac tattgcttgg gaatgttcca aaacaaatga catgctatat tcgagagtat
     1141 cacgtggatc gagtgatcaa aaagctcgac gagatgtgtg atttagactc cttttttctg
     1201 tttctacacg gccgagctgg atccggaaaa tcagtaattg catcacaagc tctttcgaaa
     1261 tctgaccaac ttattggaat aaattatgat tcaatcgttt ggctcaaaga tagtggaaca
     1321 gctccaaaat ctacattcga tttatttacg gatattttgc tgatgctaaa aagcgaagac
     1381 gatcttctca atttcccatc ggtggagcat gtcacgtcag ttgtactcaa aaggatgatc
     1441 tgcaacgcac tcattgatcg tccaaatact ttattcgtat ttgatgacgt agttcaagaa
     1501 gaaacaattc gttgggctca ggagctacgt cttcgatgtc ttgtaactac tcgtgacgtg
     1561 gaaatatcaa atgctgcttc tcaaacatgc gaattcattg aagtgacatc attggaaatc
     1621 gatgaatgtt atgattttct agaagcttat ggaatgccga tgcctgttgg agaaaaagaa
     1681 gaagatgtgc ttaataaaac aatcgaacta agcagtggaa atccagcaac gcttatgatg
     1741 tttttcaagt cttgtgaacc gaaaacattt gaaaaaatgg cacagcttaa taacaaattg
     1801 gaaagtcgag gattagtcgg tgttgaatgt atcacccctt actcgtacaa gtcactcgca
     1861 atggctcttc aaagatgtgt tgaagttttg tcagatgagg atcgaagtgc tcttgctttc
     1921 gcagttgtga tgcctcctgg agttgatata cccgtcaagc tatggtcatg tgttattcca
     1981 gttgatattt gttcaaatga agaagaacaa ttggatgatg aagttgcgga tcggttgaaa
     2041 agactcagca aacgtggagc tcttctcagt ggaaaacgaa tgcccgtttt gacattcaaa
     2101 attgatcata ttatccatat gttcttgaaa cacgtcgttg atgcacaaac tatcgccaat
     2161 ggaatctcaa ttctcgagca gcgtcttctt gaaataggaa acaataatgt atcagtaccg
     2221 gagcgacata taccatcaca tttccaaaaa ttccgtcgtt catcagccag tgagatgtat
     2281 ccaaaaacta cagaagaaac tgtgatccgt cctgaagact tcccaaagtt catgcaattg
     2341 caccagaaat tctatgactc cctcaaaaat tttgcatgct gttaannnct gcagatatcc
     2401 atcacactgg cggccgctcg agtagatgac tagtctagag ggccctattc tatagtgtca
     2461 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     2521 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     2581 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     2641 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     2701 tgcggtgggc tctatggctt ctgaggcgga aagaaccagt ggcggtaata cggttatcca
     2761 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2821 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2881 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2941 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3001 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3061 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3121 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3181 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3241 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     3301 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3361 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3421 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3481 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3541 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taacctgagg
     3601 ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg
     3661 gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg
     3721 gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa
     3781 caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg
     3841 tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat
     3901 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
     3961 catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg
     4021 tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
     4081 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     4141 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     4201 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     4261 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag
     4321 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tgatcatcct gatcgacaag
     4381 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     4441 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     4501 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     4561 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     4621 ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc
     4681 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     4741 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     4801 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     4861 atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     4921 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     4981 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     5041 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     5101 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     5161 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc
     5221 ctcaggactc ttcctttttc aataaatcaa tctaaagtat atatgagtaa acttggtctg
     5281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5581 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6241 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     6301 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6361 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6421 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6481 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6541 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6601 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6661 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6721 cgcttacaat ttac
//
