LOCUS       V008197                 6593 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008197
VERSION     V008197
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6593)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6593)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        638..656
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             700..723
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             739..2247
                     /gene="ced-3"
                     /label="Cell death protein 3"
                     /note="Cell death protein 3 from Caenorhabditis elegans.
                     Accession#: P42573"
     promoter        complement(2308..2326)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2352..2576
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2708..3296)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    complement(3625..3672)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(3907..4692)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4727..5084)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             complement(5147..6004)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6005..6109)
                     /label="AmpR promoter"
     rep_origin      6136..6591
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccggatc ccccgggaat caaaacatgg attacaaaga cgatgacgat
      721 aaagaattca ctgcagagat gatgcgtcaa gatagaagga gcttgctaga gaggaacatt
      781 atgatgttct ctagtcatct aaaagtcgat gaaattctcg aagttctcat cgcaaaacaa
      841 gtgttgaata gtgataatgg agatatgatt aattcatgtg gaacggttcg cgagaagaga
      901 cgggagatcg tgaaagcagt gcaacgacgg ggagatgtgg cgttcgacgc gttttatgat
      961 gctcttcgct ctacgggaca cgaaggactt gctgaagttc ttgaacctct cgccagatct
     1021 gttgactcga atgctgtcga attcgagtgt ccaatgtcac cggcaagcca tcgtcggagc
     1081 cgcgcattga gccccgccgg ctacacttca ccgacccgag ttcaccgtga cagcgtctct
     1141 tcagtgtcat cattcacttc ttatcaggat atctactcaa gagcaagatc tcgttctcga
     1201 tcgcgtgcac ttcattcatc ggatcgacac aattattcat ctcctccagt caacgcattt
     1261 cccagccaac cttcatccgc caactcttca ttcaccggat gctcttctct cggatacagt
     1321 tcaagtcgta atcgctcatt cagcaaagct tctggaccaa ctcaatacat attccatgaa
     1381 gaggatatga actttgtcga tgcaccaacc ataagccgtg ttttcgacga gaaaaccatg
     1441 tacagaaact tctcgagtcc tcgtggaatg tgcctcatca taaataatga acactttgag
     1501 cagatgccaa cacggaatgg taccaaggcc gacaaggaca atcttaccaa tttgttcaga
     1561 tgcatgggct atacggttat ttgcaaggac aatctgacgg gaaggggaat gctcctgaca
     1621 attcgagact ttgccaaaca cgaatcacac ggagattctg cgatactcgt gattctatca
     1681 cacggagaag agaatgtgat tattggagtt gatgatatac cgattagtac acacgagata
     1741 tatgatcttc tcaacgcggc aaatgctccc cgtctggcga ataagccgaa aatcgttttt
     1801 gtgcaggctt gtcgaggcga acgtcgtgac aatggattcc cagtcttgga ttctgtcgac
     1861 ggagttcctg catttcttcg tcgtggatgg gacaatcgag acgggccatt gttcaatttt
     1921 cttggatgtg tgcggccgca agttcagcaa gtgtggagaa agaagccgag ccaagctgac
     1981 attctgattc gatacgcaac gacagctcaa tatgtttcgt ggagaaacag tgctcgtgga
     2041 tcatggttca ttcaagccgt ctgtgaagtg ttctcgacac acgcaaagga tatggatgtt
     2101 gttgagctgc tgactgaagt caataagaag gtcgcttgtg gatttcagac atcacaggga
     2161 tcgaatattt tgaaacagat gccagagatg acatcccgcc tgctcaaaaa gttctacttt
     2221 tggccggaag cacgaaactc tgccgtctaa aattcactgc agagatctgc ggccgctcga
     2281 gtagatgact agtctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg
     2341 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     2401 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     2461 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     2521 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     2581 tgaggcggaa agaaccagtg gcggtaatac ggttatccac agaatcaggg gataacgcag
     2641 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2701 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2761 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2821 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2881 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2941 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3001 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3061 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3121 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     3181 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3241 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3301 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3361 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3421 gttttaaatc aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc
     3481 cgacgttggc tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg
     3541 aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc
     3601 cctgggaccg aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc
     3661 tttttattgc cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta
     3721 gcctccccct agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     3781 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     3841 cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc
     3901 cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     3961 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     4021 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     4081 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
     4141 cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg
     4201 cgcgagcccc tgatgctctt gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     4261 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     4321 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     4381 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     4441 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     4501 tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     4561 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     4621 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     4681 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcgatctttg caaaagccta
     4741 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
     4801 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
     4861 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     4921 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
     4981 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     5041 accctaactg acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca
     5101 ataaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5161 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5221 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5281 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5341 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5401 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5461 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5521 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5581 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5641 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5701 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5761 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5821 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5881 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5941 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6001 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6061 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6121 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6181 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6241 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6301 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6361 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6421 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6481 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6541 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tac
//
