LOCUS       V008200                 5771 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008200
VERSION     V008200
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Pirtskhalaishvili G, Shurin GV, Gambotto A, Esche C, Wahl M,
            Yurkovetsky ZR, Robbins PD, Shurin MR.
  TITLE     Transduction of dendritic cells with Bcl-xL increases their
            resistance to prostate cancer-induced apoptosis and antitumor effect
            in mice
  JOURNAL   J. Immunol. 165 (4), 1956-1964 (2000)
   PUBMED   10925278
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Gambotto A, Pagliano O, Shurin M, Robbins PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1998) Vector Core Facility, University of
            Pittsburgh, 300 Technology Drive, Pittsburgh, PA 15219, USA
REFERENCE   3  (bases 1 to 5771)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5771)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Immunol."; date: "2000"; volume: "165"; issue: "4"; pages:
            "1956-1964"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-APR-1998) Vector Core Facility, University of Pittsburgh, 300
            Technology Drive, Pittsburgh, PA 15219, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        638..656
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             747..1445
                     /gene="Bcl2l1"
                     /label="Bcl-2-like protein 1"
                     /note="Bcl-2-like protein 1 from Mus musculus. Accession#:
                     Q64373"
     polyA_signal    1530..1754
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1886..2474)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    complement(2803..2850)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(3085..3870)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(3905..4262)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             complement(4325..5182)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5183..5287)
                     /label="AmpR promoter"
     rep_origin      5314..5769
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca ctagtccagt
      721 gtggtggaat tcggctttca gagctcatgt ctcagagcaa ccgggagctg gtggtcgact
      781 ttctctccta caagctttcc cagaaaggat acagctggag tcagtttagt gatgtcgaag
      841 agaataggac tgaggcccca gaagaaactg aagcagagag ggagaccccc agtgccatca
      901 atggcaaccc atcctggcac ctggcggata gcccggccgt gaatggagcc actggccaca
      961 gcagcagttt ggatgcgcgg gaggtgattc ccatggcagc agtgaagcaa gcgctgagag
     1021 aggcaggcga tgagtttgaa ctgcggtacc ggagagcgtt cagtgatcta acatcccagc
     1081 ttcacataac cccagggacc gcgtatcaga gctttgagca ggtagtgaat gaactctttc
     1141 gggatggagt aaactggggt cgcatcgtgg cctttttctc ctttggcggg gcactgtgcg
     1201 tggaaagcgt agacaaggag atgcaggtat tggtgagtcg gattgcaagt tggatggcca
     1261 cctatctgaa tgaccaccta gagccttgga tccaggagaa cggcggctgg gacacttttg
     1321 tggatctcta cgggaacaat gcagcagccg agagccggaa aggccaggag cgcttcaacc
     1381 gctggttcct gacgggcatg actgtggctg gtgtggttct gctgggctca ctcttcagtc
     1441 ggaagtgaaa gccgaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg
     1501 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     1561 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     1621 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     1681 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     1741 ggtgggctct atggcttctg aggcggaaag aaccagtggc ggtaatacgg ttatccacag
     1801 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1861 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1921 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1981 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2041 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2101 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2161 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2221 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2281 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2341 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2401 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2461 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2521 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2581 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta
     2641 tggcagggcc tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg
     2701 ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg
     2761 tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac
     2821 cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
     2881 ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc
     2941 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     3001 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
     3061 tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     3121 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     3181 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     3241 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     3301 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct
     3361 ggcgaacagt tcggctggcg cgagcccctg atgctcttga tcatcctgat cgacaagacc
     3421 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     3481 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     3541 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     3601 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     3661 cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg acaggtcggt
     3721 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     3781 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     3841 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
     3901 gatctttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
     3961 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     4021 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     4081 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     4141 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     4201 agcctgggga ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc
     4261 aggactcttc ctttttcaat aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4381 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4441 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4501 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4561 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4621 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4681 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4741 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4801 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4861 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4921 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4981 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5041 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5101 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5161 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5281 ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg
     5341 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5401 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5461 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5521 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5581 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5641 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5701 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     5761 ttacaattta c
//
