LOCUS       40924_13215        5162 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCR-Zeo, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Yan MY, Yan HQ, Ren GX, Zhao JP, Guo XP, Sun YC.
  TITLE     CRISPR-Cas12a-assisted recombineering in bacteria
  JOURNAL   Appl. Environ. Microbiol. (2017) In press
  PUBMED    28646112
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Yan M-Y., Yan H-Q., Ren G-X., Zhao J-P., Guo X-P., Sun Y-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2017) Moh Key Laboratory of Systems Biology of 
            Pathogens, Chinese Academy of Medical Sciences and Peking Union 
            Medical College Institute of Pathogen Biology, 9, Dongdan Santiao, 
            Dongcheng District, Beijing 100730, China
REFERENCE   3  (bases 1 to 5162)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5162)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JUN-2017) Moh Key Laboratory of Systems Biology of Pathogens, 
            Chinese Academy of Medical Sciences and Peking Union Medical College
            Institute of Pathogen Biology, 9, Dongdan Santiao, Dongcheng 
            District, Beijing 100730, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      564..610
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      747..903
                     /label=pHsp70
                     /note="pHsp70"
                     /regulatory_class="promoter"
     CDS             904..1275
                     /label=BleoR
                     /note="antibiotic-binding protein"
     rep_origin      1465..4218
                     /gene="PAL5000ts"
                     /label=oriM
                     /note="oriM"
     gene            1465..4218
                     /gene="PAL5000ts"
                     /label=PAL5000ts
     rep_origin      4357..4945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 caacgcgccc gctttgatcg gggacgtctg cggccgacca tttacgggtc ttgttgtcgt
       61 tggcggtcat gggccgaaca tactcacccg gatcggaggg ccgaggacaa ggtcgaacga
      121 ggggcatgac ccggtgcggg gcttcttgca ctcggcatag gcgagtgcta agaataacgt
      181 tggcactcgc gaccggtgag tgctaggtcg ggacggtgag gccaggcccg tcgtcgcagc
      241 gagtggcagc gaggacaact tgagccgtcc gtcgcgggca ctgcgcccgg ccagcgtaag
      301 tagcggggtt gccgtcaccc ggtgaccccc gtttcatccc cgatccggag gaatcacttc
      361 gcaatggcca agacaattgc ggatccagct gcagaatttg gcgccagagg cacttcgagg
      421 ctgatttagg caaaaacggg tctaagaact ttaaataatt tctactgttg tagattccgt
      481 ctccggcctc cagtagtatt cggcgaagct tgtctaagaa ctttaaataa tttctactgt
      541 tgtagatatc gactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      601 ttcgttttat gccatcatgg ccgcgggcta gcttatcacc agcccgtcat cgtcaacgcc
      661 tgatccgcgg tgcggacagg ccgtgtcgcg accggccgtg cggaattaag ccggcccgta
      721 ccctgtgaat agaggtccgc tcataggaat tccgacccgc acgaccagcg ttagcatgct
      781 cagtaagttg agtgcatcag gctcagctct gaattgacag cacaccgccg tcgaggcaag
      841 cttgagcggg gtgcactcat catagtgcag gaaagaagct ctacatattc aggaggattc
      901 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
      961 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     1021 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     1081 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     1141 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     1201 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     1261 gccgaggagc aggactgacc gacgccgacc aacaccgccg gtccgacggc ggcccacggg
     1321 tcccaggggg gtcgacctcg aaacttgtcc aagtaccgcc acctaacata cgttgcgtcg
     1381 agagacgaag gcaccacgat taccggcgac gatgatctat tgccggtcta caccgacgct
     1441 ggggtgattg cgtttgctcc gggaacaccg atcgcttcgt tcaaaatcgg cggaagatac
     1501 cccgtgcgtg cggtgctgaa ggaccgcaaa gatgttccgt acggcaccga ggtctgcaag
     1561 tacgggatga aatccggcga gacctgtggg ccggtcgttg tggctgccaa gtacacgatc
     1621 accgctcagc tgcgcaccgt acatggcgac tcaggcgcgc cgctgtaccg caagaaccgc
     1681 gacggcacag ccgatttgat cggcatcgct tcgagcgtta acgaggacag tcgcacgacg
     1741 aagttcttct ggatcgcgcc cgtgctggaa gcactcaacc tcgaagcgtg tggttgcgga
     1801 gccatctagc aaccacacga aacatgcgca acgaaccgcg caacgaacaa cgcctagaac
     1861 tggccctaga tgagctgact cgtatcgttg gtaaacctag tttgaccagc atgttttaac
     1921 tacgttcggt gagctgtcaa cggggcctgt aacggcacaa cgaaccgtgc aacgagagtg
     1981 gccacggatg ccaccacagg cactacaacg gagttcgcca cgtacatcac cacaaccacc
     2041 gattctggcg gtgagctccc cgatattcag cggaaatggc ttggtatcga ccaagattcg
     2101 tagaaccccg tctcgtctgg ctggtattca aaacgggcgc aacgaaacac gcaacgagac
     2161 aggcatggcc caaaccagaa aactagcgtc taccaggact tttacctgtc cgacccgttg
     2221 caacggaacc ccccacggaa cccccgcgac acccgctccc caattgcgtt agaacagcgg
     2281 tggattgtcg gcttcgttgt gggccttttg agccgcttcc tgttctgccg cacgctcttt
     2341 cctcgcccga tagccgagtc gcttaacggt gtccagatgc agcccgaaat gtttggccgt
     2401 ttgcggccaa gagtggccct cgtcgtcgtg ataggcgcgg atgcgttcgc ggcgtgcagc
     2461 ctgctcggcg agccactcgc tgcgttcctg cgccacgagc cggacgacgt ggcgttcgga
     2521 tagtccggtg attcgagcgc cttcggcggc ggtcacgcgc cgctttttgc ggacagtcgg
     2581 ctgccggttg tagccgtcgc tgtagccgtc gctgtagccg tcgctcatag caatgcctcc
     2641 atggctgacg cggactttgc gcgccgcgca actgtgctcg ccgccgtgcg cgctgctgcg
     2701 cccttccgcg agatggccga ctggcgcgca ctgagtgtgg cctcgtagac cacgatcccg
     2761 tccgcccaaa tgcgcgactt ggttgtgatc caacgccaaa tgctgttggc gatggcgcgg
     2821 acctcgctgt ccggtagcgg tccgggacac acgtcgttgc acgggaattc ggcgtttcgc
     2881 gcgtggcact cggcatagat cgcgcggccg agtccgtcca cgttccgggt cggcaggtag
     2941 atccgcatga gggcgggacg ataggcccac aacctgacgg aatcgaacag tgcgcaattc
     3001 cgccctagcg gcgtcggagc cgctttgtac gtggtctgct gacgccagcg cggcggtggc
     3061 atgttcgcgc cgagctcggc ctcgatgtgg ctgagtgtgt agagatctga gtggagccat
     3121 tccgtttccc aggcgatgtg gccggggttt ttggtcatga ggcctgagta actgcggtcg
     3181 ccgtcgacgg cgcgccgaag gccttcggcg cacgccgcca tgtatgcgag cggcttacgc
     3241 cgcgcgtatt cggtgcgtgg aacaggggcg ttgagtgccc acactgcgtg tgcgtggccg
     3301 ttggcgcgat tgcccacgat cgcgttgggc agcggatggg acccccgggc gctgagcgct
     3361 cggagcgctg cgtctggatg gtctacgtcc acgaccagca ggtttgccag cgctgttggg
     3421 ttcgcctcga tgtaccggcg gcctagggcc gacgcgcggc tttggcggta gatcccctcg
     3481 agcagatcgt cgcttgccag cggccagtac ggcagccaga gctgctcaaa ttcgtcggcg
     3541 acgtggctca cgcttggtag tagaccacga ttaatcaccg gtgtatggtc cgacacgagc
     3601 tccaagtcag atatttcgct gaggggccac cccacaactg cacactcccc cgctctcccg
     3661 tcgagccctg gtggtggaac accagcgaca gccgagcacc cccaaccacc tgtaccaacc
     3721 aggaggaaca catgcgtcgt ttcgaggacg tttccgggcc gctgagagcc gctgtggcgg
     3781 ccgtacacgc cgccttagac ccgttagacc ccctgccgcc tgaatgcgcg ggtacgagcc
     3841 acacagcgcc cgaacttacg gagctggtgg gctcacctgg ctttatggcg tacgaatcgg
     3901 ctgtgtgcga cctgttgggc gaggtgaggt acgcgctact cacgctggca agggcgacac
     3961 agccgcccca ccgagcccgc acggccgcgc gcggtgtcaa caaccgggtg agtcgtgcac
     4021 accagcaggt gttcgaggct tggctcgaag tgcaggacat cgtggcgaac gccgcccgat
     4081 gagccgcgcc ttacgctggc tgccagccgt tcgcgggctg gttggtgcag cgcgtcgagc
     4141 ggttagaggc cctgcggtgt tccaccaccg caggcctcgc cctttttaag gctgaatttg
     4201 cttgtctccg aatccaactg gcttgtccaa gggtgtatct acgcttaatc caaagttcaa
     4261 acgaggggat tacacatgac caacttcgat aacgttctcg gctcgatact cgttccactg
     4321 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4381 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4441 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4501 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4561 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4621 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4681 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4741 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4801 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4861 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4921 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4981 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     5041 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     5101 cgagcggccc tagttggcat agcggccgca atcatggtac ccggcattct agacggtgac
     5161 ca
//
