LOCUS       40924_13190        3931 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Parental bacterial expression vector for TOPO(R) TA cloning of PCR 
            products.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(365..383)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(390..406)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      547..975
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             1319..2110
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2131..2988
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3162..3750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttaagggc
      301 gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag agggcccaat
      361 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      601 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      661 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      721 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      781 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      841 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      901 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      961 aatttaacgc gaattttaac aaaattcagg gcgcaagggc tgctaaagga agcggaacac
     1021 gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat
     1081 ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg
     1141 gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc
     1201 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag
     1261 gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat
     1321 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     1381 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     1441 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     1501 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     1561 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     1621 tctcctgtca tcccaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     1681 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     1741 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     1801 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     1861 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1921 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1981 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     2041 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     2101 cgagttcttc tgaattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     2161 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2221 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2281 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2341 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     2401 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2461 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2521 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2581 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2641 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     2701 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     2761 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     2821 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     2881 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2941 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     3001 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3061 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3181 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3241 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3301 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3361 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3421 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3481 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3541 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3601 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3661 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3721 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3781 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3841 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3901 cgcagcgagt cagtgagcga ggaagcggaa g
//
