LOCUS       V012900                 8780 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012900
VERSION     V012900
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(564..1220)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1221..1323)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      1875..2097
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2145..3092
                     /label="Rep101(Ts)"
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
     CDS             complement(4016..4360)
                     /gene="mbeC"
                     /label="Mobilization protein MbeC"
                     /note="Mobilization protein MbeC from Escherichia coli.
                     Accession#: P13657"
     CDS             complement(4560..5417)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5418..5522)
                     /label="AmpR promoter"
     CDS             complement(5800..6828)
                     /label="Cre"
                     /note="site-specific recombinase"
     protein_bind    complement(6854..6870)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6878..6908)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     promoter        7219..7296
                     /label="lacI promoter"
     CDS             7297..8376
                     /label="lacI"
                     /note="lac repressor"
ORIGIN
        1 gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca
       61 cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact
      121 ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg
      181 gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga cctcagcgcc
      241 acaggtgcgg ttgctggcgc taaccgtttt tatcaggctc tgggaggcag aataaatgat
      301 catatcgtca attattacct ccacggggag agcctgagca aactggcctc aggcatttga
      361 gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata
      421 agcggctatt taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt
      481 ctgccattca tccgcttatt atcacttatt caggcgtagc aaccaggcgt ttaagggcac
      541 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
      601 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
      661 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
      721 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
      781 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
      841 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
      901 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
      961 ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc
     1021 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     1081 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     1141 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     1201 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     1261 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga
     1321 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca
     1381 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg
     1441 tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
     1501 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
     1561 caaaagcacc gccggacatc agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     1621 ccgggggact gttgggcgcc tgtagtgcca tttaccccca ttcactgcca gagccgtgag
     1681 cgcagcgaac tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg tcggagacaa
     1741 aaggaatatt cagcgatttg cccgagcttg cgagggtgct acttaagcct ttagggtttt
     1801 aaggtctgtt ttgtagagga gcaaacagcg tttgcgacat ccttttgtaa tactgcggaa
     1861 ctgactaaag tagtgagtta tacacagggc tgggatctat tctttttatc tttttttatt
     1921 ctttctttat tctataaatt ataaccactt gaatataaac aaaaaaaaca cacaaaggtc
     1981 tagcggaatt tacagagggt ctagcagaat ttacaagttt tccagcaaag gtctagcaga
     2041 atttacagat acccacaact caaaggaaaa ggactagtaa ttatcattga ctagcccatc
     2101 tcaattggta tagtgattaa aatcacctag accaattgag atgtatgtct gaattagttg
     2161 ttttcaaagc aaatgaacta gcgattagtc gctatgactt aacggagcat gaaaccaagc
     2221 taattttatg ctgtgtggca ctactcaacc ccacgattga aaaccctaca aggaaagaac
     2281 ggacggtatc gttcacttat aaccaatacg ttcagatgat gaacatcagt agggaaaatg
     2341 cttatggtgt attagctaaa gcaaccagag agctgatgac gagaactgtg gaaatcagga
     2401 atcctttggt taaaggcttt gagattttcc agtggacaaa ctatgccaag ttctcaagcg
     2461 aaaaattaga attagttttt agtgaagaga tattgcctta tcttttccag ttaaaaaaat
     2521 tcataaaata taatctggaa catgttaagt cttttgaaaa caaatactct atgaggattt
     2581 atgagtggtt attaaaagaa ctaacacaaa agaaaactca caaggcaaat atagagatta
     2641 gccttgatga atttaagttc atgttaatgc ttgaaaataa ctaccatgag tttaaaaggc
     2701 ttaaccaatg ggttttgaaa ccaataagta aagatttaaa cacttacagc aatatgaaat
     2761 tggtggttga taagcgaggc cgcccgactg atacgttgat tttccaagtt gaactagata
     2821 gacaaatgga tctcgtaacc gaacttgaga acaaccagat aaaaatgaat ggtgacaaaa
     2881 taccaacaac cattacatca gattcctacc tacataacgg actaagaaaa acactacacg
     2941 atgctttaac tgcaaaaatt cagctcacca gttttgaggc aaaatttttg agtgacatgc
     3001 aaagtaagta tgatctcaat ggttcgttct catggctcac gcaaaaacaa cgaaccacac
     3061 tagagaacat actggctaaa tacggaagga tctgaggttc ttatggctct tgtatctatc
     3121 agtgaagcat caagactaac aaacaaaagt agaacaactg ttcaccgtta catatcaaag
     3181 ggaaaactgt ccatatgcac agatgaaaac ggtgtaaaaa agatagatac atcagagctt
     3241 ttacgagttt ttggtgcatt taaagctgtt caccatgaac agatcgacaa tgtaacagat
     3301 gaacagcatg taacacctaa tagaacaggt gaaaccagta aaacaaagca actagaacat
     3361 gaaattgaac acctgagaca acttgttaca gctcaacagt cacacataga cagcctgaaa
     3421 caggcgatgc tgcttatcga atcaaagctg ccgacaacac gggagccagt gacgcctccc
     3481 gtggggaaaa aatcatggca attctggaag aaatagcgcc tgtttcgttt caggcaggtt
     3541 atcagggagt gtcagcgtcc tgcggttctc cggggcgttc gggtcatgca gcccgtaatg
     3601 gtgatttacc agcgtctgcc aggcatcaat tctaggcctg tctgcgcggt cgtagtacgg
     3661 ctggaggcgt tttccggtct gtagctccat gttcggaatg acaaaattca gctcaagccg
     3721 tcccttgtcc tggtgctcca cccacaggat gctgtactga tttttttcga gaccgggcat
     3781 cagtacacgc tcaaagctcg ccatcacttt ttcacgtcct cccggcggca gctccttctc
     3841 cgcgaacgac agaacaccgg acgtgtattt cttcgcaaat ggcgtggcat cgatgagttc
     3901 ccggacttct tccggattac cctgaagcac cgttgcgcct tcgcggttac gctccctccc
     3961 cagcaggtaa tcaaccggac cactgccacc accttttccc ctggcatgaa atttaactat
     4021 catcccgcgc cccctgttcc ctgacagcca gacgcagccg gcgcagctca tccccgatgg
     4081 ccatcagtgc ggccaccacc tgaacccggt caccggaaga ccactgcccg ctgttcacct
     4141 tacgggctgt ctgattcagg ttatttccga tggcggccag ctgacgcagt aacggcggtg
     4201 ccagtgtcgg cagttttccg gaacgggcaa ccggctcccc caggcagacc cgccgcatcc
     4261 ataccgccag ttgtttaccc tcacagcgtt caagtaaccg ggcatgttca tcatcagtaa
     4321 cccgtattgt gagcatcctc tcgcgtttca tcggtatcat taccccatga acagaaatcc
     4381 cccttacacg gaggcatcag tgactaaacg gggtctgacg ctcagtggaa cgaaaactca
     4441 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4501 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4561 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4621 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4681 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4741 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4801 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4861 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4921 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4981 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5041 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5101 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5161 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5221 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5281 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5341 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5401 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5461 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5521 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     5581 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaatttta taaaccgtgg
     5641 agcgggcaat actgagctga tgagcaattt ccgttgcacc agtgcccttc tgatgaagcg
     5701 tcagcacgac gttcctgtcc acggtacgcc tgcggccaaa tttgattcct ttcagctttg
     5761 cttcctgtcg gccctcattc gtgcgctcta ggatccctaa tcgccatctt ccagcaggcg
     5821 caccattgcc cctgtttcac tatccagggt acggatatag ttcatgacaa tatttacatt
     5881 ggtccagcca ccagcttgca tgatctccgg tattgaaact ccagcgcggg ccatatctcg
     5941 cgcggctccg acacgggcac tgtgtccaga ccaggccagg tatctctgac cagagtcatc
     6001 cttagcgccg taaatcaatc gatgagttgc ttcaaaaatc ccttccaggg cgcgagttga
     6061 tagctggctg gtggcagatg gcgcggcaac accatttttt ctgacccggc aaaacaggta
     6121 gttattcgga tcatcagcta caccagagac ggaaatccat cgctcgacca gtttagttac
     6181 ccccaggcta agtgccttct ctacacctgc ggtgctaacc agcgttttcg ttctgccaat
     6241 atggattaac attctcccac cgtcagtacg tgagatatct ttaaccctga tcctggcaat
     6301 ttcggctata cgtaacaggg tgttataagc aatccccaga aatgccagat tacgtatatc
     6361 ctggcagcga tcgctatttt ccatgagtga acgaacctgg tcgaaatcag tgcgttcgaa
     6421 cgctagagcc tgttttgcac gttcaccggc atcaacgttt tcttttcgga tccgccgcat
     6481 aaccagtgaa acagcattgc tgtcacttgg tcgtggcagc ccggaccgac gatgaagcat
     6541 gtttagctgg cccaaatgtt gctggatagt ttttactgcc agaccgcgcg cctgaagata
     6601 tagaagataa tcgcgaacat cttcaggttc tgcgggaaac catttccggt tattcaactt
     6661 gcaccatgcc gcccacgacc ggcaaacgga cagaagcatt ttccaggtat gctcagaaaa
     6721 cgcctggcga tccctgaaca tgtccatcag gttcttgcga acctcatcac tcgttgcatc
     6781 gaccggtaat gcaggcaaat tttggtgtac ggtcagtaaa ttggacatgg tgaattccag
     6841 cacacccgcg gccttgttat ccgctcacaa ttccacacat tatacgagcc ggaagcataa
     6901 agtgtaaagc ctggggtgcc taaacgatct cgatcctcta cgccggacgc atcgtggccg
     6961 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     7021 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     7081 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     7141 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     7201 gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga
     7261 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat
     7321 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc
     7381 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt
     7441 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc
     7501 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat
     7561 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa
     7621 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg
     7681 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt
     7741 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga
     7801 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca
     7861 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat
     7921 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa
     7981 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag
     8041 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggacatc
     8101 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc
     8161 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     8221 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     8281 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8341 ctggcacgac aggtttcccg actggaaagc gggcagtgac ccggggatcg cagtggtgag
     8401 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     8461 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     8521 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
     8581 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     8641 atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa caccccttgt
     8701 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
     8761 aatgtaacat cagagatttt
//
