LOCUS       V008338                 8429 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008338
VERSION     V008338
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R,
            King S, Hazbon MH.
  TITLE     Sequencing of a set of cassette-based shuttle vectors for
            Gram-positive bacteria deposited at BEI resources from the NARSA
            collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8429)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R,
            King S, Hazbon MH.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2015) BEI Bacteriology, BEI Resources/American
            Type and Culture Collection (ATCC), 10801 University Boulevard,
            Manassas, VA 20110-2209, USA
REFERENCE   3  (bases 1 to 8429)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8429)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0.2
            Coverage              :: 25047
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-MAY-2015) BEI Bacteriology, BEI Resources/American Type and
            Culture Collection (ATCC), 10801 University Boulevard, Manassas, VA
            20110-2209, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..2089
                     /note="pT181copwt repC; Staphylococcus replication
                     cassette; derived from J04764"
     misc_feature    159..392
                     /label="RS-B site"
                     /note="RS-B site"
     rep_origin      656..823
                     /label="derived from Staphylococcus aureus"
                     /note="derived from Staphylococcus aureus"
     CDS             688..1632
                     /codon_start=1
                     /product="RepC protein"
                     /label="RepC protein"
                     /note="polypeptide A"
                     /protein_id="AMR43675.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
     variation       1132..1134
                     /label="E149D"
                     /note="E149D"
     misc_feature    1794..2089
                     /label="truncated polypeptide B"
                     /note="truncated polypeptide B"
     misc_feature    2408..2467
                     /label="leader peptide"
                     /note="leader peptide"
     CDS             2527..3258
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     misc_feature    3364..4503
                     /note="phi11 EcoRI-K; derived from AF42478"
     misc_feature    complement(3364..3483)
                     /label="truncated RinA"
                     /note="truncated RinA"
     CDS             complement(3654..3842)
                     /codon_start=1
                     /product="RinB"
                     /label="RinB"
                     /protein_id="AMR43677.1"
                     /translation="MGCLVVVKEILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSD
                     FAKLSDQSDLMRAEVSE"
     CDS             complement(4190..4363)
                     /codon_start=1
                     /product="phi SLT ORF 81b-like protein"
                     /label="phi SLT ORF 81b-like protein"
                     /protein_id="AMR43676.1"
                     /translation="MVLYKELDLFTCRIGMLLVLIVIVDFAKQKNMLATLSVVLILLFV
                     EKLRIIQRSDKK"
     promoter        4758..4862
                     /label="AmpR promoter"
     CDS             4863..5720
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5894..6482
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             6834..7199
                     /gene="cadC"
                     /label="Cadmium resistance transcriptional regulatory
                     protein CadC"
                     /note="Cadmium resistance transcriptional regulatory
                     protein CadC from Staphylococcus aureus. Accession#:
                     P20047"
     misc_feature    7205..7249
                     /note="polylinker; derived from M13mp19"
     CDS             7279..8121
                     /gene="blaZ"
                     /label="Beta-lactamase"
                     /note="Beta-lactamase from Staphylococcus aureus.
                     Accession#: P00807"
     misc_feature    8288..8425
                     /label="TT"
                     /note="TT"
ORIGIN
        1 cctttgcgga aagagttaat aagttaacag aagatgaacc aaaactaaat ggtttagcag
       61 gaaacttaga taaaaaaatg aatccagaat tatattcaga acaggaacag caacaagaac
      121 aacaaaagaa tcaaaaacga gatagaggta tgcacttata gaacatgcat ttatgccgag
      181 aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt tggttggact
      241 tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc ctgtaggttc
      301 cgaccaataa ggaaattgga ataaagcaat aaaaggagtt gaagaaatga aattcagaga
      361 agcctttgag aattttataa caagtaagta tgtacttggt gttttagtag ttttaactgt
      421 ttaccagata atacaaatgc ttaaataaaa aaagacttga tctgattaga ccaaatcttt
      481 tgatagtgtt atattaataa caaaataaaa aggagtcgct cacgccctga ccaaagtttg
      541 tgaacgacat cattcaaaga aaaaaacact gagttgtttt tataatcttg tatatttaga
      601 tattaaacga tatttaaata tacatcaaga tatatatttg ggtgagcgat tccttaaacg
      661 aaattgagat taaggagtcg attttttatg tataaaaaca atcatgcaaa tcattcaaat
      721 catttggaaa atcacgattt agacaatttt tctaaaaccg gctactctaa tagccggttg
      781 gacgcacata ctgtgtgcat atctgatcca aaattaagtt ttgatgcaat gacgatcgtt
      841 ggaaatctca accgagacaa cgctcaagcc ctttctaaat ttatgagtgt agagccccaa
      901 ataagacttt gggatattct tcaaacaaag tttaaagcta aagcacttca agaaaaagtt
      961 tatattgaat atgacaaagt gaaagcagat agttgggata gacgtaatat gcgtattgaa
     1021 tttaatccaa acaaacttac acgagatgaa atgatttggt taaaacaaaa tataataagc
     1081 tacatggaag atgacggttt tacaagatta gatttagcct ttgattttga agatgatttg
     1141 agtgactact atgcaatgtc tgataaagca gttaagaaaa ctatttttta tggtcgtaat
     1201 ggtaagccag aaacaaaata ttttggcgtg agagatagta atagatttat tagaatttat
     1261 aataaaaagc aagaacgtaa agataatgca gatgctgaag ttatgtctga acatttatgg
     1321 cgtgtagaaa tcgaacttaa aagagatatg gtggattact ggaatgattg ctttagtgat
     1381 ttacatatct tgcaaccaga ttggaaaact atccaacgca ctgcggatag agcaatagtt
     1441 tttatgttat tgagtgatga agaagaatgg ggaaagcttc acagaaattc tagaacaaaa
     1501 tataagaatt tgataaaaga aatttcgcca gtcgatttaa cggacttaat gaaatcgact
     1561 ttaaaagcga acgaaaaaca attgcaaaaa caaatcgatt tttggcaaca tgaatttaaa
     1621 ttttggaaat agtgtacata ttaatattac tgaacaaaaa tgatatattt aaactattct
     1681 aatttaggag gattttttta tgaagtgtct atttaaaaat ttggggaatt tatatgaggt
     1741 gaaagaataa tttaccccta taaactttag tcacctcaag taaagaggta aaattgttta
     1801 gtttatataa aaaatttaaa ggtttgtttt atagcgtttt attttggctt tgtattcttt
     1861 cattttttag tgtattaaat gaaatggttt taaatgtttc tttacctgat attgcaaatc
     1921 attttaatac tactcctgga attacaaact gggtaaacac tgcatatatg ttaacttttt
     1981 cgataggaac agcagtatat ggaaaattat ctgattatat aaatataaaa aaattgttaa
     2041 ttattggtat tagtttgagc tgtcttggtt cattgattgc ttttattggg cccacctagg
     2101 aattgaatga gacatgctac acctccggat aataaatata tataaacgta tatagatttc
     2161 ataaagtcta acacactaga cttatttact tcgtaattaa gtcgttaaac cgtgtgctct
     2221 acgaccaaaa ctataaaacc tttaagaact ttcttttttt acaagaaaaa agaaattaga
     2281 taaatctctc atatctttta ttcaataatc gcatccgatt gcagtataaa tttaacgatc
     2341 actcatcatg ttcatattta tcagagctcg tgctataatt atactaattt tataaggagg
     2401 aaaaaatatg ggcattttta gtatttttgt aatcagcaca gttcattatc aaccaaacaa
     2461 aaaataagtg gttataatga atcgttaata agcaaaattc atataaccaa attaaagagg
     2521 gttataatga acgagaaaaa tataaaacac agtcaaaact ttattacttc aaaacataat
     2581 atagataaaa taatgacaaa tataagatta aatgaacatg ataatatctt tgaaatcggc
     2641 tcaggaaaag gccattttac ccttgaatta gtaaagaggt gtaatttcgt aactgccatt
     2701 gaaatagacc ataaattatg caaaactaca gaaaataaac ttgttgatca cgataatttc
     2761 caagttttaa acaaggatat attgcagttt aaatttccta aaaaccaatc ctataaaata
     2821 tatggtaata taccttataa cataagtacg gatataatac gcaaaattgt ttttgatagt
     2881 atagctaatg agatttattt aatcgtggaa tacgggtttg ctaaaagatt attaaataca
     2941 aaacgctcat tggcattact tttaatggca gaagttgata tttctatatt aagtatggtt
     3001 ccaagagaat attttcatcc taaacctaaa gtgaatagct cacttatcag attaagtaga
     3061 aaaaaatcaa gaatatcaca caaagataaa caaaagtata attatttcgt tatgaaatgg
     3121 gttaacaaag aatacaagaa aatatttaca aaaaatcaat ttaacaattc cttaaaacat
     3181 gcaggaattg acgatttaaa caatattagc tttgaacaat tcttatctct tttcaatagc
     3241 tataaattat ttaataagta agttaaggga tgcataaact gcatccctta acttgttttt
     3301 cgtgtgccta ttttttgtga atcgattatg tcttttgcgc agtcggctta aaccagtttt
     3361 ccgcggtgtc attatttcac ttcttaaatc ttcaagttgt ttatgataat taggataatc
     3421 acacaactca tcttctaact ttcgaactgt tgataatttt aatccgtatt tctttttagt
     3481 catgaatacc ctccgtacaa atatgtttaa tcttcaaagt gtctcaatct acttcttaat
     3541 atctctatct ctcgctcttt aacttttaca tcacctttta actgttccgc ttgtaacatc
     3601 acaccaaaca ataagatgac tagtaatata attgctatga ttaaccacat catctactcc
     3661 gacacctccg ccctcatcaa atcagactga tcactcaact ttgcgaagtc acttggcgcc
     3721 tctacatcat cattagccgt catcataata tatacttgct cagttacata cttacctaac
     3781 tcatacatcg ctagtaagaa taatagtctc aaaatttctt taaccaccac taaacacccc
     3841 atgttaattt atcgataatt tgtatagctt gttttaatgc gtctcttttt tctttgatat
     3901 ctctattatc gccatcttca tcagctgaca ttaactcact gtcatattca tataatagtt
     3961 ctgatatttc attactagct actactaata agttttcatc tacatcaatc gttaccgttt
     4021 tctttggcat ctccatctct ccttatctta acttgtgcct cgtatttgcg ctcagcttct
     4081 tctttactct ctgcctcaac aactgtaaac gtctgattat ctctagcagt agtaaaatgt
     4141 tcatgtggtt gtcctgttga atctttgaat gttgtgacta agtattgcgt cacttcttat
     4201 cactcctttg aatgattcta agtttttcta cgaataaaag tattagtaca acactcaatg
     4261 tagccaacat atttttttgc tttgcaaaat ctactataac gattaagact aataacattc
     4321 caattctgca tgtaaataaa tctaattctt tgtacaaaac catatatctg ttaagtaaat
     4381 tgttaaatat tactatgaat acaagtattg gaactaatgt aatgatgtaa ctcacttccc
     4441 caaaacctcc ttgactcgat ctaagatgtc tttacactcc gctacttccg aagcctttta
     4501 ctcgagcggc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     4561 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     4621 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     4681 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     4741 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4801 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4861 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4921 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4981 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5041 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5101 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5161 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5221 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5281 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5341 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5401 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5461 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5521 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5581 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5641 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5701 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5761 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5821 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5881 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5941 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6001 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6061 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6121 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6181 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6241 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6301 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6361 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6421 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6481 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6541 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6601 ctggcggccg ctgcatgcgc acttattcaa gtgtattttt taataaatta ttttacttat
     6661 tgaaatgtat tattttctaa tgtcataccc tggtcaaaac cgttcgtttt tgagactaga
     6721 attttatgcc ctacttactt cttttatttt cattcaaata tttgcttgca tgatgagtcg
     6781 aaaatggtta taatacactc aaataaatat ttgaatgaag atgggatgat aatatgaaaa
     6841 agaaagatac ttgtgaaatt ttttgttatg acgaagaaaa ggttaatcga atacaagggg
     6901 atttacaaac agttgatatt tctggtgtta gccaaatttt aaaggctatt gccgatgaaa
     6961 atagagcaaa aattacttac gctctgtgtc aggatgaaga gttgtgtgtt tgtgatatag
     7021 caaatatctt aggtgttacg atagcaaatg catctcatca tttacgtacg ctttataagc
     7081 aaggggtggt caactttaga aaagaaggaa aactagcttt atattcttta ggtgatgaac
     7141 atatcaggca gataatgatg atcgccctag cacataagaa agaagtgaag gtcaatgtct
     7201 gaacctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattcg ttaactaatt
     7261 aatatcggag ggtttatttt gaaaaagtta atatttttaa ttgtaattgc tttagtttta
     7321 agtgcatgta attcaaacag ttcacatgcc aaagagttaa atgatttaga aaaaaaatat
     7381 aatgctcata ttggtgttta tgctttagat actaaaagtg gtaaggaagt aaaatttaat
     7441 tcagataaga gatttgccta tgcttcaact tcaaaagcga taaatagtgc tattttgtta
     7501 gaacaagtac cttataataa gttaaataaa aaagtacata ttaacaaaga tgatatagtt
     7561 gcttattctc ctattttaga aaaatatgta ggaaaagata tcactttaaa agcacttatt
     7621 gaggcttcaa tgacatatag tgataataca gcaaacaata aaattataaa agaaatcggt
     7681 ggaatcaaaa aagttaaaca acgtctaaaa gaactaggag ataaagtaac aaatccagtt
     7741 agatatgaga tagaattaaa ttactattca ccaaagagca aaaaagatac ttcaacacct
     7801 gctgctttcg gtaagacttt aaataaactt atcgcaaatg gaaaattaag caaagaaaac
     7861 aaaaaattct tacttgattt aatgttaaat aataaaagcg gagatacttt aattaaagac
     7921 ggtgttccaa aagactataa ggttgctgat aaaagtggtc aagcaataac atatgcttct
     7981 agaaatgatg ttgcttttgt ttatcctaag ggccaatctg aacctattgt tttagtcatt
     8041 tttacgaata aagacaataa aagtgataag ccaaatgata agttgataag tgaaaccgcc
     8101 aagagtgtaa tgaaggaatt ttaatattct aaatgcataa taaatactga taacatctta
     8161 tattttgtat tatattttgt attatcgttg acatgtataa ttttgatatc aaaaactgat
     8221 tttccctcta ttattttcga gatttatttt cttaattctc tttaacaaac tagaaatatt
     8281 gtatatacaa aaaattataa ataatagatg aatagtttaa ttataggtgt tcatcaatcg
     8341 aaaaagcaac gtatcttatt taaagtgcgt tgcttttttc tcatttataa ggttaaataa
     8401 ttctcatata tcaagcaaag tgacaggcg
//
